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The map label for this gene is leuO [H]

Identifier: 59713217

GI number: 59713217

Start: 35233

End: 36129

Strand: Reverse

Name: leuO [H]

Synonym: VF_A0034

Alternate gene names: 59713217

Gene position: 36129-35233 (Counterclockwise)

Preceding gene: 59713219

Following gene: 59713216

Centisome position: 2.72

GC content: 41.92

Gene sequence:

>897_bases
ATGGTTTCAAATATAGATCTAAACTTATTAAAAGTGTTTGTGGTGGTTTATCGCCACCAGTCGATTACTTTAGCCGCAGA
AGAAATGGGATTAACGCAACCGGGAGTAAGCGGTTTACTTAAGCGTTTACAACAACAGGTGGGAAGTCAGCTGTTTATTC
GTTCAGGACGAGGGATTATTCCAACACAGCATGCTCAAGAATTAATTCGTCATGTTGAGCCTGCATTAGCTCAAATCAAC
AACGCATTAGAAGGGTTAGAGGGCTTTTCGACCCAGTATCCACGCAAGTTCGTTATATATACCTCAGAGCCTGTAATGTT
AATGCTGCTACCGAAGTTGGAAGCAGACAGAAGCTTGGGTAATGTCACTATTGAGTTGCACCCGACGCACTCTAATGAAG
AAGCGCTAATTCAAGCACTCAATCAACATCAAGCTGATCTCGCTATTGAAATATCCAACTATTCAACAGGCTCATTTTTT
ACCGAACATTTATTTGATGATCAGCTCCGGCTCATTGCTAGGAAAAAGCATCCAAGATTGACAGGTGGCGTTACTCGTGA
GCAGTTTTATGCCGAGAAACACATTACGTTAAAGTTACGTCGTGAAGATACCTACATGGCAGATTATTTTACCGAGGAAC
GACTAAGCGAACGACAAGTAGCCGCAGAGTGCACCTCTCTTGTGTCATTAATGTCGATGGTCGCGACAAGTGATTGCATT
GCGATGGTGACCGAAAGTATCGCAACCGCATTTGCTGATAAACTGGATATACAAATGTTAGATTGCCCATTTACATCTAA
ACCAGTTAAGTATCGTTTATTAACCCATAATCGAGAGCGACAAAGACCGGCAAATATGTGGTTGCGAGAAAAAATAAAGT
CCTACTTTTCAGGCTAA

Upstream 100 bases:

>100_bases
ATTCGTATTGGTATGGAGCTAATTTAGCGCATTGACACATAGCGATACATGCATGAAACTGTATTTAACACATCACGAAA
TGTTATGTTTGAGGGAAAGG

Downstream 100 bases:

>100_bases
TAACTCTATTAATATAGTATTTTCCTCAAAAACGCGCATAATCTTGCGATACAACACTCAAAAGAGAAATGACCTATGAC
TAATTTTCAATACTACTTTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MVSNIDLNLLKVFVVVYRHQSITLAAEEMGLTQPGVSGLLKRLQQQVGSQLFIRSGRGIIPTQHAQELIRHVEPALAQIN
NALEGLEGFSTQYPRKFVIYTSEPVMLMLLPKLEADRSLGNVTIELHPTHSNEEALIQALNQHQADLAIEISNYSTGSFF
TEHLFDDQLRLIARKKHPRLTGGVTREQFYAEKHITLKLRREDTYMADYFTEERLSERQVAAECTSLVSLMSMVATSDCI
AMVTESIATAFADKLDIQMLDCPFTSKPVKYRLLTHNRERQRPANMWLREKIKSYFSG

Sequences:

>Translated_298_residues
MVSNIDLNLLKVFVVVYRHQSITLAAEEMGLTQPGVSGLLKRLQQQVGSQLFIRSGRGIIPTQHAQELIRHVEPALAQIN
NALEGLEGFSTQYPRKFVIYTSEPVMLMLLPKLEADRSLGNVTIELHPTHSNEEALIQALNQHQADLAIEISNYSTGSFF
TEHLFDDQLRLIARKKHPRLTGGVTREQFYAEKHITLKLRREDTYMADYFTEERLSERQVAAECTSLVSLMSMVATSDCI
AMVTESIATAFADKLDIQMLDCPFTSKPVKYRLLTHNRERQRPANMWLREKIKSYFSG
>Mature_298_residues
MVSNIDLNLLKVFVVVYRHQSITLAAEEMGLTQPGVSGLLKRLQQQVGSQLFIRSGRGIIPTQHAQELIRHVEPALAQIN
NALEGLEGFSTQYPRKFVIYTSEPVMLMLLPKLEADRSLGNVTIELHPTHSNEEALIQALNQHQADLAIEISNYSTGSFF
TEHLFDDQLRLIARKKHPRLTGGVTREQFYAEKHITLKLRREDTYMADYFTEERLSERQVAAECTSLVSLMSMVATSDCI
AMVTESIATAFADKLDIQMLDCPFTSKPVKYRLLTHNRERQRPANMWLREKIKSYFSG

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=294, Percent_Identity=27.2108843537415, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1786820, Length=257, Percent_Identity=29.5719844357977, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI87081766, Length=265, Percent_Identity=29.4339622641509, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1790147, Length=306, Percent_Identity=25.1633986928105, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788297, Length=262, Percent_Identity=25.5725190839695, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787601, Length=295, Percent_Identity=22.0338983050847, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33926; Mature: 33926

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSNIDLNLLKVFVVVYRHQSITLAAEEMGLTQPGVSGLLKRLQQQVGSQLFIRSGRGII
CCCCCHHHHHHHHHHHHHCCCEEEEHHHCCCCCCCHHHHHHHHHHHHCCCEEHHCCCCCC
PTQHAQELIRHVEPALAQINNALEGLEGFSTQYPRKFVIYTSEPVMLMLLPKLEADRSLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCC
NVTIELHPTHSNEEALIQALNQHQADLAIEISNYSTGSFFTEHLFDDQLRLIARKKHPRL
CEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCC
TGGVTREQFYAEKHITLKLRREDTYMADYFTEERLSERQVAAECTSLVSLMSMVATSDCI
CCCCHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMVTESIATAFADKLDIQMLDCPFTSKPVKYRLLTHNRERQRPANMWLREKIKSYFSG
HHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEECCCCHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVSNIDLNLLKVFVVVYRHQSITLAAEEMGLTQPGVSGLLKRLQQQVGSQLFIRSGRGII
CCCCCHHHHHHHHHHHHHCCCEEEEHHHCCCCCCCHHHHHHHHHHHHCCCEEHHCCCCCC
PTQHAQELIRHVEPALAQINNALEGLEGFSTQYPRKFVIYTSEPVMLMLLPKLEADRSLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCC
NVTIELHPTHSNEEALIQALNQHQADLAIEISNYSTGSFFTEHLFDDQLRLIARKKHPRL
CEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCC
TGGVTREQFYAEKHITLKLRREDTYMADYFTEERLSERQVAAECTSLVSLMSMVATSDCI
CCCCHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMVTESIATAFADKLDIQMLDCPFTSKPVKYRLLTHNRERQRPANMWLREKIKSYFSG
HHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEECCCCHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3519576 [H]