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The map label for this gene is ilvA [H]

Identifier: 59713167

GI number: 59713167

Start: 2882341

End: 2883873

Strand: Direct

Name: ilvA [H]

Synonym: VF_2560

Alternate gene names: 59713167

Gene position: 2882341-2883873 (Clockwise)

Preceding gene: 59713166

Following gene: 59713168

Centisome position: 99.48

GC content: 40.9

Gene sequence:

>1533_bases
ATGAGCAACTCTTCATATCAAACTGGCGCAGAATATCTGCGTCAGATCTTACGAGCACCAATCTATGAAGTGGCAACAGT
AACACCACTTCAAGATATGCCTCGTTTATCTGAGCGCATTAACAACCGAGTTCAGTTAAAGCGAGAAGACCGTCAACCGG
TTCACTCCTTTAAACTTCGTGGTGCTTACAATATGGTTGCAAATCTAACCCAAGAGCAAAAAAATGCTGGGGTTATTGCT
GCATCTGCTGGTAATCACGCTCAAGGTATGGCGCTTTCAGGAACCAAGCTGGGCATTCAAACGACAATAGTGATGCCCAA
AACGACACCGGATATCAAAGTTGAAGCCGTAAAAGGTTTTGGTGGAAATGTGGTTCTACATGGCAGTAATTTCGATGAAG
CCAAAGCAGAAGCAGAACGTTTATCACAACTCCACGGTTATACTTTTGTACCTCCGTTTGATCACCCACTGGTGATTGCA
GGTCAAGGAACAATAGGAATGGAGATGCTGCAACAAAATGGTCATCTCGATTATATCTTTGTACCTGTTGGTGGTGGTGG
TTTAGCTGCTGGTATTGCAGTGCTAATAAAACAACTCATGCCTGAAATAAAAGTTATCGCTGTTGAGCCTGAAGACTCTT
CTTGTTTAAAAGCGGCTTTAGATGCTGGTGAGCCTGTCATTCTTGATCAAGTGAGTATGTTTGCCGATGGTGTCGCTGTT
AAACGCATTGGTGATGAAACTTTCCGTCTATGTCAAAAATACCTTGATGGCCATATCAGCGTATCAAGTGATGAAATTTG
TGCAGCGGTAAAAGATATTTTTGAAGATACAAGAGCGATTGCAGAACCCTCTGGTGCATTGGCATTAGCTGGTCTGAAAA
AATTTGCAGATCAACATCAATTAGAAAACAAACAACTAGGAACCGTACTTTCTGGTGCAAATACTAACTTCCATGGTTTA
CGTTATGTATCTGAGCGCTGTGAGTTAGGTGAAAAACGAGAAGGGCTATTGGCTGTAACGATTCCGGAACGTCAGGGCGC
TTTCTTTGAATTTTGTAATCTAATTGGTGGCAGAGCTGTTACTGAATTTAACTACCGCTATAACGACGATAAGCTAGCTA
ACATTTTTGTTGGTGTTCGCTTACAAGAAGGGCAAGAAGAGCTTGATAACATCATTAATGATCTACAAAAGGGAGAATAC
CCGGTTGTTGATTTATCTGATGATGAAATGGCTAAGCTGCATATACGTTATATGATTGGTGGTAAACCATCAAAAACAAT
TCAAGAACGTTTATACAGTTTTGAATTTCCAGAATACCCTGGAGCGCTCGTTCGCTTCTTAAGTACTCTAGGTACTCACT
GGAATATCAGTCTCTTCAATTATCGTAACCACGGTGCAGATTATGGACGTGTGTTATGTGGTTTTGAGCTAACTGATTCT
GATCTTGCTAGTTTTTCAAAACACCTTGAAGAGCTTGGTTATCAATGGAAAGATGAGACAGATAATCTAGCTTATCGATT
CTTTTTATCCTAA

Upstream 100 bases:

>100_bases
CAACGTGAAGTCTCTTTTGCATTAAAAGCCTATGCAAGTATGGCAACAAGTGCAGATAAAGGTGCTGTTCGTGATAAATC
TAAGTTAGAAGGGTAAGCCT

Downstream 100 bases:

>100_bases
GACCGTACGGCGTAATAAGGCTAATTAAAATTTAGCCTTATTTTCATTCAAATTTGAAATTATGCTATACAATCGGTTAA
AAAATGCTATTATTTTGCTC

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MSNSSYQTGAEYLRQILRAPIYEVATVTPLQDMPRLSERINNRVQLKREDRQPVHSFKLRGAYNMVANLTQEQKNAGVIA
ASAGNHAQGMALSGTKLGIQTTIVMPKTTPDIKVEAVKGFGGNVVLHGSNFDEAKAEAERLSQLHGYTFVPPFDHPLVIA
GQGTIGMEMLQQNGHLDYIFVPVGGGGLAAGIAVLIKQLMPEIKVIAVEPEDSSCLKAALDAGEPVILDQVSMFADGVAV
KRIGDETFRLCQKYLDGHISVSSDEICAAVKDIFEDTRAIAEPSGALALAGLKKFADQHQLENKQLGTVLSGANTNFHGL
RYVSERCELGEKREGLLAVTIPERQGAFFEFCNLIGGRAVTEFNYRYNDDKLANIFVGVRLQEGQEELDNIINDLQKGEY
PVVDLSDDEMAKLHIRYMIGGKPSKTIQERLYSFEFPEYPGALVRFLSTLGTHWNISLFNYRNHGADYGRVLCGFELTDS
DLASFSKHLEELGYQWKDETDNLAYRFFLS

Sequences:

>Translated_510_residues
MSNSSYQTGAEYLRQILRAPIYEVATVTPLQDMPRLSERINNRVQLKREDRQPVHSFKLRGAYNMVANLTQEQKNAGVIA
ASAGNHAQGMALSGTKLGIQTTIVMPKTTPDIKVEAVKGFGGNVVLHGSNFDEAKAEAERLSQLHGYTFVPPFDHPLVIA
GQGTIGMEMLQQNGHLDYIFVPVGGGGLAAGIAVLIKQLMPEIKVIAVEPEDSSCLKAALDAGEPVILDQVSMFADGVAV
KRIGDETFRLCQKYLDGHISVSSDEICAAVKDIFEDTRAIAEPSGALALAGLKKFADQHQLENKQLGTVLSGANTNFHGL
RYVSERCELGEKREGLLAVTIPERQGAFFEFCNLIGGRAVTEFNYRYNDDKLANIFVGVRLQEGQEELDNIINDLQKGEY
PVVDLSDDEMAKLHIRYMIGGKPSKTIQERLYSFEFPEYPGALVRFLSTLGTHWNISLFNYRNHGADYGRVLCGFELTDS
DLASFSKHLEELGYQWKDETDNLAYRFFLS
>Mature_509_residues
SNSSYQTGAEYLRQILRAPIYEVATVTPLQDMPRLSERINNRVQLKREDRQPVHSFKLRGAYNMVANLTQEQKNAGVIAA
SAGNHAQGMALSGTKLGIQTTIVMPKTTPDIKVEAVKGFGGNVVLHGSNFDEAKAEAERLSQLHGYTFVPPFDHPLVIAG
QGTIGMEMLQQNGHLDYIFVPVGGGGLAAGIAVLIKQLMPEIKVIAVEPEDSSCLKAALDAGEPVILDQVSMFADGVAVK
RIGDETFRLCQKYLDGHISVSSDEICAAVKDIFEDTRAIAEPSGALALAGLKKFADQHQLENKQLGTVLSGANTNFHGLR
YVSERCELGEKREGLLAVTIPERQGAFFEFCNLIGGRAVTEFNYRYNDDKLANIFVGVRLQEGQEELDNIINDLQKGEYP
VVDLSDDEMAKLHIRYMIGGKPSKTIQERLYSFEFPEYPGALVRFLSTLGTHWNISLFNYRNHGADYGRVLCGFELTDSD
LASFSKHLEELGYQWKDETDNLAYRFFLS

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia. Deaminates L-threonine, but also L-serine to a lesser extent [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=296, Percent_Identity=33.445945945946, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI19923959, Length=273, Percent_Identity=32.2344322344322, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI33469958, Length=325, Percent_Identity=28, Blast_Score=100, Evalue=6e-21,
Organism=Escherichia coli, GI1790207, Length=502, Percent_Identity=69.9203187250996, Blast_Score=709, Evalue=0.0,
Organism=Escherichia coli, GI1789505, Length=295, Percent_Identity=39.3220338983051, Blast_Score=187, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI71991565, Length=329, Percent_Identity=33.1306990881459, Blast_Score=155, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17537387, Length=321, Percent_Identity=35.5140186915888, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17508781, Length=292, Percent_Identity=34.9315068493151, Blast_Score=144, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6320930, Length=514, Percent_Identity=49.0272373540856, Blast_Score=469, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6322631, Length=306, Percent_Identity=36.2745098039216, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6319788, Length=264, Percent_Identity=25.3787878787879, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21355833, Length=310, Percent_Identity=34.5161290322581, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24645328, Length=251, Percent_Identity=30.6772908366534, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 56409; Mature: 56278

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSSYQTGAEYLRQILRAPIYEVATVTPLQDMPRLSERINNRVQLKREDRQPVHSFKLR
CCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHEECC
GAYNMVANLTQEQKNAGVIAASAGNHAQGMALSGTKLGIQTTIVMPKTTPDIKVEAVKGF
CHHHHHHHHHHHHCCCCEEEECCCCCCCCEEECCCEECEEEEEEECCCCCCEEEEEEECC
GGNVVLHGSNFDEAKAEAERLSQLHGYTFVPPFDHPLVIAGQGTIGMEMLQQNGHLDYIF
CCCEEEECCCCCHHHHHHHHHHHHCCCEECCCCCCCEEEECCCCHHHHHHHCCCCEEEEE
VPVGGGGLAAGIAVLIKQLMPEIKVIAVEPEDSSCLKAALDAGEPVILDQVSMFADGVAV
EEECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCEEHHHHHHHHCCHHH
KRIGDETFRLCQKYLDGHISVSSDEICAAVKDIFEDTRAIAEPSGALALAGLKKFADQHQ
HHCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LENKQLGTVLSGANTNFHGLRYVSERCELGEKREGLLAVTIPERQGAFFEFCNLIGGRAV
HCCHHHHHEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEE
TEFNYRYNDDKLANIFVGVRLQEGQEELDNIINDLQKGEYPVVDLSDDEMAKLHIRYMIG
EEECCEECCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEEEEC
GKPSKTIQERLYSFEFPEYPGALVRFLSTLGTHWNISLFNYRNHGADYGRVLCGFELTDS
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEEECCHH
DLASFSKHLEELGYQWKDETDNLAYRFFLS
HHHHHHHHHHHCCCCCCCCCCCEEEEEEEC
>Mature Secondary Structure 
SNSSYQTGAEYLRQILRAPIYEVATVTPLQDMPRLSERINNRVQLKREDRQPVHSFKLR
CCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHEECC
GAYNMVANLTQEQKNAGVIAASAGNHAQGMALSGTKLGIQTTIVMPKTTPDIKVEAVKGF
CHHHHHHHHHHHHCCCCEEEECCCCCCCCEEECCCEECEEEEEEECCCCCCEEEEEEECC
GGNVVLHGSNFDEAKAEAERLSQLHGYTFVPPFDHPLVIAGQGTIGMEMLQQNGHLDYIF
CCCEEEECCCCCHHHHHHHHHHHHCCCEECCCCCCCEEEECCCCHHHHHHHCCCCEEEEE
VPVGGGGLAAGIAVLIKQLMPEIKVIAVEPEDSSCLKAALDAGEPVILDQVSMFADGVAV
EEECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCEEHHHHHHHHCCHHH
KRIGDETFRLCQKYLDGHISVSSDEICAAVKDIFEDTRAIAEPSGALALAGLKKFADQHQ
HHCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LENKQLGTVLSGANTNFHGLRYVSERCELGEKREGLLAVTIPERQGAFFEFCNLIGGRAV
HCCHHHHHEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEE
TEFNYRYNDDKLANIFVGVRLQEGQEELDNIINDLQKGEYPVVDLSDDEMAKLHIRYMIG
EEECCEECCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEEEEC
GKPSKTIQERLYSFEFPEYPGALVRFLSTLGTHWNISLFNYRNHGADYGRVLCGFELTDS
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEEECCHH
DLASFSKHLEELGYQWKDETDNLAYRFFLS
HHHHHHHHHHHCCCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3315862; 3550695; 1379743; 9278503; 3003115; 2473940; 9562556 [H]