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The map label for this gene is ilvE [H]

Identifier: 59713165

GI number: 59713165

Start: 2879530

End: 2880477

Strand: Direct

Name: ilvE [H]

Synonym: VF_2558

Alternate gene names: 59713165

Gene position: 2879530-2880477 (Clockwise)

Preceding gene: 59713164

Following gene: 59713166

Centisome position: 99.38

GC content: 42.62

Gene sequence:

>948_bases
ATGTCAAAGACAGCAGATTTTATTTGGTTTAATGGTGAGATGGTTCCTTGGGCAGACGCAAATGTGCACGTTCTGACTCA
CGCAATGCATTATGGAACATCCGTTTTTGAAGGGGTGCGTTGTTATAACACGCCAAATGGTCCAATTATTTTCCGCCACC
CAGAGCACGCAAAACGTTTAAAAAATTCCGCTAAAATTTATCGCTTCCCAATTCCATATACTGAAGAAGAAATTATGGAA
GCAACACGTGAAACCTTACGTCAAAACAAACTCGATTCTGCTTATATTCGTCCTTTGGGCTTTGTAGGAAATGTTGGTTT
AGGTGTTTGCCCACCAGAAGATACGGAAATGGAACTCATTATTGCTGCATTCCCATGGGGATCTTACCTAGGAGAAGAAG
CATTAGAAAATGGGGTTGATGCCATGATTTCAAGTTGGAACCGTGCTGCACCAAATACCATTCCAACCGCAGCAAAAGCA
GGTGGTAACTATTTATCTTCTTTATTGGTTGGTGGTGAAGCCCGTCGTCATGGTTACGATGAAGGTATTGCTTTAAGTGT
TGATGGCTATCTTTCTGAAGGAGCTGGTGAAAACCTATTTGTTGTTCGTGATGGTATTCTTTCTACGCCACCTGCAACCA
GTGCCATCTTACCGGGAATAACTCGAGATTCTATCATGACGCTTGCAAAAGATATGGGGTATGAAATTCGTGAAGAAAAC
ATCGCACGCGAAGCGCTATATCTTGCAGACGAAGTATTTATGACAGGCACAGCGGCTGAAATCGTTCCAGTACGCTCTGT
TGACAAGATTACCGTTGGTGAAGGTAAACGTGGTCCTATCACAAAAGAGATCCAAGCCGCTTACTTTGGTCTGTTCAATG
GAACAACCGAAGATAAATGGGGTTGGTTAGATTACGTATACCCAGAAAATGCACCATCAGCTAAATAA

Upstream 100 bases:

>100_bases
TGACCGACCAATTAGCCTTCTTACTAATCAAATAGAAAAGCTTTGGGATATTACCGCCGTTAACGTTTCTACATTACATC
GCGCATAAGGATTAGTGACA

Downstream 100 bases:

>100_bases
TTTATAAGGATATAAGAATGCCAACTTATCGTTCAGCCACCACCACTCACGGACGTAACATGGCCGGAGCACGCGCTTTA
TGGCGTGCAACTGGCGTTAA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSKTADFIWFNGEMVPWADANVHVLTHAMHYGTSVFEGVRCYNTPNGPIIFRHPEHAKRLKNSAKIYRFPIPYTEEEIME
ATRETLRQNKLDSAYIRPLGFVGNVGLGVCPPEDTEMELIIAAFPWGSYLGEEALENGVDAMISSWNRAAPNTIPTAAKA
GGNYLSSLLVGGEARRHGYDEGIALSVDGYLSEGAGENLFVVRDGILSTPPATSAILPGITRDSIMTLAKDMGYEIREEN
IAREALYLADEVFMTGTAAEIVPVRSVDKITVGEGKRGPITKEIQAAYFGLFNGTTEDKWGWLDYVYPENAPSAK

Sequences:

>Translated_315_residues
MSKTADFIWFNGEMVPWADANVHVLTHAMHYGTSVFEGVRCYNTPNGPIIFRHPEHAKRLKNSAKIYRFPIPYTEEEIME
ATRETLRQNKLDSAYIRPLGFVGNVGLGVCPPEDTEMELIIAAFPWGSYLGEEALENGVDAMISSWNRAAPNTIPTAAKA
GGNYLSSLLVGGEARRHGYDEGIALSVDGYLSEGAGENLFVVRDGILSTPPATSAILPGITRDSIMTLAKDMGYEIREEN
IAREALYLADEVFMTGTAAEIVPVRSVDKITVGEGKRGPITKEIQAAYFGLFNGTTEDKWGWLDYVYPENAPSAK
>Mature_314_residues
SKTADFIWFNGEMVPWADANVHVLTHAMHYGTSVFEGVRCYNTPNGPIIFRHPEHAKRLKNSAKIYRFPIPYTEEEIMEA
TRETLRQNKLDSAYIRPLGFVGNVGLGVCPPEDTEMELIIAAFPWGSYLGEEALENGVDAMISSWNRAAPNTIPTAAKAG
GNYLSSLLVGGEARRHGYDEGIALSVDGYLSEGAGENLFVVRDGILSTPPATSAILPGITRDSIMTLAKDMGYEIREENI
AREALYLADEVFMTGTAAEIVPVRSVDKITVGEGKRGPITKEIQAAYFGLFNGTTEDKWGWLDYVYPENAPSAK

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI50658084, Length=293, Percent_Identity=28.3276450511945, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI296010904, Length=329, Percent_Identity=26.7477203647416, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI296010906, Length=329, Percent_Identity=26.7477203647416, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI38176287, Length=329, Percent_Identity=26.7477203647416, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI258614015, Length=260, Percent_Identity=29.2307692307692, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI48994963, Length=305, Percent_Identity=71.1475409836066, Blast_Score=473, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI17565728, Length=282, Percent_Identity=28.3687943262411, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17568601, Length=286, Percent_Identity=27.972027972028, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322608, Length=331, Percent_Identity=27.4924471299094, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6322002, Length=287, Percent_Identity=27.5261324041812, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24641779, Length=319, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34589; Mature: 34458

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTADFIWFNGEMVPWADANVHVLTHAMHYGTSVFEGVRCYNTPNGPIIFRHPEHAKRL
CCCCCCEEEECCEECCCCCCCCEEEHHHHHHCHHHHCCEEEEECCCCCEEEECCHHHHHH
KNSAKIYRFPIPYTEEEIMEATRETLRQNKLDSAYIRPLGFVGNVGLGVCPPEDTEMELI
HCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEE
IAAFPWGSYLGEEALENGVDAMISSWNRAAPNTIPTAAKAGGNYLSSLLVGGEARRHGYD
EEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHCCCHHHCCCC
EGIALSVDGYLSEGAGENLFVVRDGILSTPPATSAILPGITRDSIMTLAKDMGYEIREEN
CCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCHHHHHH
IAREALYLADEVFMTGTAAEIVPVRSVDKITVGEGKRGPITKEIQAAYFGLFNGTTEDKW
HHHHHHHHHHHHHCCCCCHHEEECCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCC
GWLDYVYPENAPSAK
CEEEEECCCCCCCCC
>Mature Secondary Structure 
SKTADFIWFNGEMVPWADANVHVLTHAMHYGTSVFEGVRCYNTPNGPIIFRHPEHAKRL
CCCCCEEEECCEECCCCCCCCEEEHHHHHHCHHHHCCEEEEECCCCCEEEECCHHHHHH
KNSAKIYRFPIPYTEEEIMEATRETLRQNKLDSAYIRPLGFVGNVGLGVCPPEDTEMELI
HCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEE
IAAFPWGSYLGEEALENGVDAMISSWNRAAPNTIPTAAKAGGNYLSSLLVGGEARRHGYD
EEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHCCCHHHCCCC
EGIALSVDGYLSEGAGENLFVVRDGILSTPPATSAILPGITRDSIMTLAKDMGYEIREEN
CCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCHHHHHH
IAREALYLADEVFMTGTAAEIVPVRSVDKITVGEGKRGPITKEIQAAYFGLFNGTTEDKW
HHHHHHHHHHHHHCCCCCHHEEECCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCC
GWLDYVYPENAPSAK
CEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]