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The map label for this gene is ilvB [H]

Identifier: 59713163

GI number: 59713163

Start: 2877566

End: 2879272

Strand: Direct

Name: ilvB [H]

Synonym: VF_2556

Alternate gene names: 59713163

Gene position: 2877566-2879272 (Clockwise)

Preceding gene: 172087786

Following gene: 59713164

Centisome position: 99.31

GC content: 43.64

Gene sequence:

>1707_bases
ATGGGGGCACATGATGTGCGAGAGAAAACAGACAACCACTCACGCAGGGAGCGAAAGAGTATGAATGGCTCACAGTTAGT
AGTAAAAGCCTTAAGAGACGAAGGAATTGAAACCGTCTTTGGTTATCCTGGTGGGGCTATCATGCCTATTTATGATGCAT
TATATGATGGTGGCGTTGAGCACATATTATGTCGTCATGAACAAGGTGCAGCAATGGCTGCAATAGGAATGGCAAGAGCA
AGCAAAGATGTTGCTGTATGCCTTGCAACATCAGGTCCAGGAGCAACAAACCTAATTACGGGTCTTGCTGATGCCATGTT
AGATTCCATTCCTCTTGTTGCTATCACAGGTCAAGTGGCGAGTAATTTAATCGGTACTGATGCTTTCCAAGAAATGGATG
TCATCGGTATGTCTCTCTCATGTACTAAACACAGTTATTTGGTTACTGATATCAATGAACTTGCACCTGTGATTGCTGAA
GCATTTGAGGTAGCAAAATCAGGACGTCCCGGCCCTGTATTAATTGATATAGCCAAAGACGTACAACTCGCTGAAGCTCC
TGTTACTTCTCTTCCATATTTTGAAGCACCTAGTATCCCTGTTGTTGTTCCTGAAGCTATTGAAGCGGCTGAGGCATTAC
TAAGAGCCAGCAACAAACCGGTATTGTATGTGGGTGGCGGTGTTCAACTGGCACACGCAACCGACTCTGTTCGTGATTTT
TTAACCAATAATCCGATGCCTTCAGTAAGTACATTAAAAGGTTTAGGTTCAATAGGAAGACACGATCCCCATTATCTTGG
AATGGTAGGTATGCACGGCACTAAAGCTGCCAACCTAATTGTTCAAGAATCTGATTTACTTATTGTTGTTGGTGCTCGTT
TTGATGATCGTGTGACAGGAAAATTAGAAAGCTTTGCTCCTCATGCGAAAGTAATCCATATCGATATTGATGCAGCAGAA
TTCAATAAGCTTCGCCACGCTCATTCAACACTACGCGGTGACATAAAGGTTATCCTTCCTCAATTGAAACTAGAGCAGGA
CCTTACCCCTTGGTTAGAACATGTTTCTTCTCTTCGCTCTGAATTTAAATGGCGCTACGATCACCCGGGTGAATTAATTT
ATGCCCCTCTGCTACTAAAGCAACTTTCTGACATGATGCCAGACAGCGCTATCGTATCGACAGATGTAGGCCAACATCAA
ATGTGGGCAGCTCAACATATCCAACCTCGAGCTCCTGAAAATTACCTCACTTCCGCAGGTCTTGGCACCATGGGGTTCGG
TTTACCCGCTGCAATGGGAGCAAAAGTGGCTCGTCCTGATGATCAATCTATTCTGATTTCAGGTGATGGTTCTTTCATGA
TGAATGTACAAGAACTCGGTACGATTAAGCGTAAACAAATCCCTGTGAAGATGGTTCTTATTAACAACCAACGTTTAGGC
ATGGTTCGCCAATGGCAATCTTTGTTTTTTGATGGCCGCCACAGTGAAACCATTCTTGATGATAATCCTGATTTTGTTGC
TCTTGCTGCCGCCTTTGGTATTCCTGGAAAAACCATCACAATAAAAGAAGAAGTAGAACCCGCTTTAAAAGAAATGCTTG
AAAGCGACACGGCTTATCTGCTGCATGTTCTTATTTCAGAAGAAGAAAACGTATGGCCTTTGGTTCCGCCAGGCGCTGCA
AACCAAGATATGGTGGAGGGAGAATAA

Upstream 100 bases:

>100_bases
CACCGCTCGGGGATGTGTGAGCAAAAAGTAGAGTTACAAGCACACTAAAAACCCCCAAGCCAACAAGCAAGGGGGTTTTT
TTATAAGTTCAGGAAGAGAA

Downstream 100 bases:

>100_bases
TGCAACGTTATATTTTAGAAATTGAAGCTAACGATAGACCGGTTTTATTAGAACGTGTATTACGCGTTATTCGTCACCGT
GGTTTTATCATCAAGCAAGT

Product: acetolactate synthase 2 catalytic subunit

Products: NA

Alternate protein names: AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit [H]

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MGAHDVREKTDNHSRRERKSMNGSQLVVKALRDEGIETVFGYPGGAIMPIYDALYDGGVEHILCRHEQGAAMAAIGMARA
SKDVAVCLATSGPGATNLITGLADAMLDSIPLVAITGQVASNLIGTDAFQEMDVIGMSLSCTKHSYLVTDINELAPVIAE
AFEVAKSGRPGPVLIDIAKDVQLAEAPVTSLPYFEAPSIPVVVPEAIEAAEALLRASNKPVLYVGGGVQLAHATDSVRDF
LTNNPMPSVSTLKGLGSIGRHDPHYLGMVGMHGTKAANLIVQESDLLIVVGARFDDRVTGKLESFAPHAKVIHIDIDAAE
FNKLRHAHSTLRGDIKVILPQLKLEQDLTPWLEHVSSLRSEFKWRYDHPGELIYAPLLLKQLSDMMPDSAIVSTDVGQHQ
MWAAQHIQPRAPENYLTSAGLGTMGFGLPAAMGAKVARPDDQSILISGDGSFMMNVQELGTIKRKQIPVKMVLINNQRLG
MVRQWQSLFFDGRHSETILDDNPDFVALAAAFGIPGKTITIKEEVEPALKEMLESDTAYLLHVLISEEENVWPLVPPGAA
NQDMVEGE

Sequences:

>Translated_568_residues
MGAHDVREKTDNHSRRERKSMNGSQLVVKALRDEGIETVFGYPGGAIMPIYDALYDGGVEHILCRHEQGAAMAAIGMARA
SKDVAVCLATSGPGATNLITGLADAMLDSIPLVAITGQVASNLIGTDAFQEMDVIGMSLSCTKHSYLVTDINELAPVIAE
AFEVAKSGRPGPVLIDIAKDVQLAEAPVTSLPYFEAPSIPVVVPEAIEAAEALLRASNKPVLYVGGGVQLAHATDSVRDF
LTNNPMPSVSTLKGLGSIGRHDPHYLGMVGMHGTKAANLIVQESDLLIVVGARFDDRVTGKLESFAPHAKVIHIDIDAAE
FNKLRHAHSTLRGDIKVILPQLKLEQDLTPWLEHVSSLRSEFKWRYDHPGELIYAPLLLKQLSDMMPDSAIVSTDVGQHQ
MWAAQHIQPRAPENYLTSAGLGTMGFGLPAAMGAKVARPDDQSILISGDGSFMMNVQELGTIKRKQIPVKMVLINNQRLG
MVRQWQSLFFDGRHSETILDDNPDFVALAAAFGIPGKTITIKEEVEPALKEMLESDTAYLLHVLISEEENVWPLVPPGAA
NQDMVEGE
>Mature_567_residues
GAHDVREKTDNHSRRERKSMNGSQLVVKALRDEGIETVFGYPGGAIMPIYDALYDGGVEHILCRHEQGAAMAAIGMARAS
KDVAVCLATSGPGATNLITGLADAMLDSIPLVAITGQVASNLIGTDAFQEMDVIGMSLSCTKHSYLVTDINELAPVIAEA
FEVAKSGRPGPVLIDIAKDVQLAEAPVTSLPYFEAPSIPVVVPEAIEAAEALLRASNKPVLYVGGGVQLAHATDSVRDFL
TNNPMPSVSTLKGLGSIGRHDPHYLGMVGMHGTKAANLIVQESDLLIVVGARFDDRVTGKLESFAPHAKVIHIDIDAAEF
NKLRHAHSTLRGDIKVILPQLKLEQDLTPWLEHVSSLRSEFKWRYDHPGELIYAPLLLKQLSDMMPDSAIVSTDVGQHQM
WAAQHIQPRAPENYLTSAGLGTMGFGLPAAMGAKVARPDDQSILISGDGSFMMNVQELGTIKRKQIPVKMVLINNQRLGM
VRQWQSLFFDGRHSETILDDNPDFVALAAAFGIPGKTITIKEEVEPALKEMLESDTAYLLHVLISEEENVWPLVPPGAAN
QDMVEGE

Specific function: Catalyzes the first step in the biosynthesis of branched-chain amino acids [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=26.3250883392226, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI21361361, Length=523, Percent_Identity=25.0478011472275, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1790104, Length=555, Percent_Identity=46.4864864864865, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=41.5194346289753, Blast_Score=433, Evalue=1e-122,
Organism=Escherichia coli, GI1786717, Length=552, Percent_Identity=30.4347826086957, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1787096, Length=542, Percent_Identity=30.0738007380074, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI1788716, Length=546, Percent_Identity=25.4578754578755, Blast_Score=136, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=580, Percent_Identity=27.0689655172414, Blast_Score=168, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17531301, Length=580, Percent_Identity=27.0689655172414, Blast_Score=168, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17542570, Length=602, Percent_Identity=24.7508305647841, Blast_Score=145, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=576, Percent_Identity=41.6666666666667, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6320816, Length=495, Percent_Identity=24.4444444444444, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI19922626, Length=580, Percent_Identity=24.8275862068966, Blast_Score=158, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61411; Mature: 61280

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAHDVREKTDNHSRRERKSMNGSQLVVKALRDEGIETVFGYPGGAIMPIYDALYDGGVE
CCCCHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCHH
HILCRHEQGAAMAAIGMARASKDVAVCLATSGPGATNLITGLADAMLDSIPLVAITGQVA
HEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEHHHH
SNLIGTDAFQEMDVIGMSLSCTKHSYLVTDINELAPVIAEAFEVAKSGRPGPVLIDIAKD
HHHHCCHHHHHHHHHCEEEECCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
VQLAEAPVTSLPYFEAPSIPVVVPEAIEAAEALLRASNKPVLYVGGGVQLAHATDSVRDF
CCHHCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHCCCCCEEEECCCEEEECCHHHHHHH
LTNNPMPSVSTLKGLGSIGRHDPHYLGMVGMHGTKAANLIVQESDLLIVVGARFDDRVTG
HHCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCEEEEEECCCCCCHHC
KLESFAPHAKVIHIDIDAAEFNKLRHAHSTLRGDIKVILPQLKLEQDLTPWLEHVSSLRS
CHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEECHHHHHCCCHHHHHHHHHHHH
EFKWRYDHPGELIYAPLLLKQLSDMMPDSAIVSTDVGQHQMWAAQHIQPRAPENYLTSAG
HCCCCCCCCCCEEHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCCCCCHHHHHHCC
LGTMGFGLPAAMGAKVARPDDQSILISGDGSFMMNVQELGTIKRKQIPVKMVLINNQRLG
CCCCCCCCHHHHCCCCCCCCCCEEEEECCCCEEEEHHHHCCHHHHCCCEEEEEECCCCCC
MVRQWQSLFFDGRHSETILDDNPDFVALAAAFGIPGKTITIKEEVEPALKEMLESDTAYL
HHHHHHHHHCCCCCCCCEECCCCCEEEEEEHHCCCCCEEEEHHHHHHHHHHHHCCCHHHE
LHVLISEEENVWPLVPPGAANQDMVEGE
EHEEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GAHDVREKTDNHSRRERKSMNGSQLVVKALRDEGIETVFGYPGGAIMPIYDALYDGGVE
CCCHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCHH
HILCRHEQGAAMAAIGMARASKDVAVCLATSGPGATNLITGLADAMLDSIPLVAITGQVA
HEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEHHHH
SNLIGTDAFQEMDVIGMSLSCTKHSYLVTDINELAPVIAEAFEVAKSGRPGPVLIDIAKD
HHHHCCHHHHHHHHHCEEEECCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
VQLAEAPVTSLPYFEAPSIPVVVPEAIEAAEALLRASNKPVLYVGGGVQLAHATDSVRDF
CCHHCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHCCCCCEEEECCCEEEECCHHHHHHH
LTNNPMPSVSTLKGLGSIGRHDPHYLGMVGMHGTKAANLIVQESDLLIVVGARFDDRVTG
HHCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCEEEEEECCCCCCHHC
KLESFAPHAKVIHIDIDAAEFNKLRHAHSTLRGDIKVILPQLKLEQDLTPWLEHVSSLRS
CHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEECHHHHHCCCHHHHHHHHHHHH
EFKWRYDHPGELIYAPLLLKQLSDMMPDSAIVSTDVGQHQMWAAQHIQPRAPENYLTSAG
HCCCCCCCCCCEEHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCCCCCHHHHHHCC
LGTMGFGLPAAMGAKVARPDDQSILISGDGSFMMNVQELGTIKRKQIPVKMVLINNQRLG
CCCCCCCCHHHHCCCCCCCCCCEEEEECCCCEEEEHHHHCCHHHHCCCEEEEEECCCCCC
MVRQWQSLFFDGRHSETILDDNPDFVALAAAFGIPGKTITIKEEVEPALKEMLESDTAYL
HHHHHHHHHCCCCCCCCEECCCCCEEEEEEHHCCCCCEEEEHHHHHHHHHHHHCCCHHHE
LHVLISEEENVWPLVPPGAANQDMVEGE
EHEEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3550695; 1379743; 9278503; 1995430; 6154938; 7015336; 3897211 [H]