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The map label for this gene is btuB [H]

Identifier: 59713042

GI number: 59713042

Start: 2734183

End: 2736030

Strand: Reverse

Name: btuB [H]

Synonym: VF_2435

Alternate gene names: 59713042

Gene position: 2736030-2734183 (Counterclockwise)

Preceding gene: 59713044

Following gene: 59713041

Centisome position: 94.43

GC content: 36.47

Gene sequence:

>1848_bases
ATGAAGAAAACCGCCTTAGCGACAGCTGTAGTGTCGCTGCTTTCACACGCCTATACATCCCCTGTATTTGCTCAAAATAC
CACAACAGAAACCGATGAAACAATGGTTGTTACTGCGAACAGATTTGAGCAGTCAACAGAAGATGTTATTGCACCAATGA
GTGTAGTCACCAAAGAAGAGATTGTTCAGATTCAAGCAAAAACGATGACGGACATTTTAAGGTTGCAACCTGGGATAGAA
GTCGCAAGCAATGGTGGTATTGGTCAAAATGCCTCTATTTTTATGAGAGGAACTAACTCTGATCATGTATTGATTTTAAT
TGATGGTTTTAGAATGAACAGTTCCATTAAAAGTGGAATGAACATTAATAAGATCCCTGTAAACCAAGTTGAAAGAATTG
AAATAATTCGTGGTTCTGGAGCTGTAATGTATGGCTCCGATGCTGTTGGTGGTGTGATTAATATCATCACTAGAACGTCT
TCTGATAATCAGTCGAAATCAGCAACAATTGGTGCTGGTAGTGATAGATATAAAGAAGGAAACTTTGCAGCTAACACTAA
GGTAGGTGAAAACGGACATTTAAAAATGGCTGCCGGATTCACCCAAACAGAAGGCTACAATGTTAATCCTCAGCCGGGAT
TAAATGATGGAGATAAACATGGCTTTGAAAATAGCCAGTTTATGTTGAACTACGAGCACCAGCTTTCTTCTTATGTAAGT
CTTTTTACTGGTGTTAGATGGTTTAATAGTACATCAGAATATAACCATTATACAAATACGAAATCTCGCTCAGAATCAGA
ATCTACGGTTTATTCTTCAAAATTATCTTTTCATAAAGATGCGGTTTCATCAAATCTAAGTATTAGTTATCAAGATGACC
AAACTTTGGAATACCATGAGAATGGAGATCTCGATTCAGAGCAAAAAATTGATCAGTTAAATATGCAATGGGCTAATCAA
ATCAAATTAACGAATGATTGGTTAGTTAATGCTGGTGTTGATTGGACTGATGCGAAAGTGAAAGCTGACATTACTGATTG
GTCAAATAAGGTAATTATTAATGAAGGTGAAAGTCGCACGTCGACTGGCGTCTATCTTGGAACTCAATATCAACTAGAGC
AATTAAAGTTAGAAGCAAATACTCGATTTGATAAGCACGATAAATATGATGAATATACAACGTGGGGTGTTGGTGCGAGT
TATCAAATTAATGATATTTACAAAGTAAGTACTGCTTATAATACAGCGTTTAAAGCGCCATCATTTTATGACTTATCGAC
AGCTCCAGATTTAGAGCCGGAAACGTCAAAAAATATTGATGTTAGTCTTGCAGCAGTATATAGCCTAGTTAATGTTAATT
TATCTCTTTATAAGAACCGTGTAGATAATTTAATTGTATATTATGACGCTCCAAATTTTGCAGGCTATAGTGCGAATGTT
GATGCTGATATTAAAGGTGCTGAGTTAGAGTTGTTATTTGATACTGGAGTTATTTCTCATACATTGATCGCTGAGTATAA
AGATCATGAGGATACAGAAGGTCAACAACTAGCAAGAAGAGCAAAACAGAATTATAAATGGATGGGTAATGTAAGTTTAG
GAGATTTAGACCTGAACCTTTCTTATATTTATACAGGAAAGCGCTCTGATTTACCTTCAGCCCCAGGTGTTGAAACCGCT
TATCTTGATCCATATTCATTGTGGAGTGTTGCTGCTGCTTATTGGGTAACGTCAGATCTTGCTGTTCGAGGTCGAGTTGA
AAACTTATTTGATGAAGAATATGAAACGGCGGGTGGCTATAAACCTGCAGAGCGCTCATATTATGTTAGTTTAGATTATC
AATTTTAA

Upstream 100 bases:

>100_bases
ACTCTAGGTTAACTGCTCTCAAGTCCGAAGACCTGCCAGCAATAAGTAATACATTACGTATTGCTATATGGACTTAACGC
GATTTTAGGAAACAACGGAA

Downstream 100 bases:

>100_bases
TTTGATTTAAGACCGCCTTCATTGGCGGTTTTTCATTTTGAGGTATGAATGAAAAAGAAAGTTATTATTAGTTGGTCATC
AGGAAAGGATTCAACACTCA

Product: vitamin B12/cobalamin outer membrane transporter

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MKKTALATAVVSLLSHAYTSPVFAQNTTTETDETMVVTANRFEQSTEDVIAPMSVVTKEEIVQIQAKTMTDILRLQPGIE
VASNGGIGQNASIFMRGTNSDHVLILIDGFRMNSSIKSGMNINKIPVNQVERIEIIRGSGAVMYGSDAVGGVINIITRTS
SDNQSKSATIGAGSDRYKEGNFAANTKVGENGHLKMAAGFTQTEGYNVNPQPGLNDGDKHGFENSQFMLNYEHQLSSYVS
LFTGVRWFNSTSEYNHYTNTKSRSESESTVYSSKLSFHKDAVSSNLSISYQDDQTLEYHENGDLDSEQKIDQLNMQWANQ
IKLTNDWLVNAGVDWTDAKVKADITDWSNKVIINEGESRTSTGVYLGTQYQLEQLKLEANTRFDKHDKYDEYTTWGVGAS
YQINDIYKVSTAYNTAFKAPSFYDLSTAPDLEPETSKNIDVSLAAVYSLVNVNLSLYKNRVDNLIVYYDAPNFAGYSANV
DADIKGAELELLFDTGVISHTLIAEYKDHEDTEGQQLARRAKQNYKWMGNVSLGDLDLNLSYIYTGKRSDLPSAPGVETA
YLDPYSLWSVAAAYWVTSDLAVRGRVENLFDEEYETAGGYKPAERSYYVSLDYQF

Sequences:

>Translated_615_residues
MKKTALATAVVSLLSHAYTSPVFAQNTTTETDETMVVTANRFEQSTEDVIAPMSVVTKEEIVQIQAKTMTDILRLQPGIE
VASNGGIGQNASIFMRGTNSDHVLILIDGFRMNSSIKSGMNINKIPVNQVERIEIIRGSGAVMYGSDAVGGVINIITRTS
SDNQSKSATIGAGSDRYKEGNFAANTKVGENGHLKMAAGFTQTEGYNVNPQPGLNDGDKHGFENSQFMLNYEHQLSSYVS
LFTGVRWFNSTSEYNHYTNTKSRSESESTVYSSKLSFHKDAVSSNLSISYQDDQTLEYHENGDLDSEQKIDQLNMQWANQ
IKLTNDWLVNAGVDWTDAKVKADITDWSNKVIINEGESRTSTGVYLGTQYQLEQLKLEANTRFDKHDKYDEYTTWGVGAS
YQINDIYKVSTAYNTAFKAPSFYDLSTAPDLEPETSKNIDVSLAAVYSLVNVNLSLYKNRVDNLIVYYDAPNFAGYSANV
DADIKGAELELLFDTGVISHTLIAEYKDHEDTEGQQLARRAKQNYKWMGNVSLGDLDLNLSYIYTGKRSDLPSAPGVETA
YLDPYSLWSVAAAYWVTSDLAVRGRVENLFDEEYETAGGYKPAERSYYVSLDYQF
>Mature_615_residues
MKKTALATAVVSLLSHAYTSPVFAQNTTTETDETMVVTANRFEQSTEDVIAPMSVVTKEEIVQIQAKTMTDILRLQPGIE
VASNGGIGQNASIFMRGTNSDHVLILIDGFRMNSSIKSGMNINKIPVNQVERIEIIRGSGAVMYGSDAVGGVINIITRTS
SDNQSKSATIGAGSDRYKEGNFAANTKVGENGHLKMAAGFTQTEGYNVNPQPGLNDGDKHGFENSQFMLNYEHQLSSYVS
LFTGVRWFNSTSEYNHYTNTKSRSESESTVYSSKLSFHKDAVSSNLSISYQDDQTLEYHENGDLDSEQKIDQLNMQWANQ
IKLTNDWLVNAGVDWTDAKVKADITDWSNKVIINEGESRTSTGVYLGTQYQLEQLKLEANTRFDKHDKYDEYTTWGVGAS
YQINDIYKVSTAYNTAFKAPSFYDLSTAPDLEPETSKNIDVSLAAVYSLVNVNLSLYKNRVDNLIVYYDAPNFAGYSANV
DADIKGAELELLFDTGVISHTLIAEYKDHEDTEGQQLARRAKQNYKWMGNVSLGDLDLNLSYIYTGKRSDLPSAPGVETA
YLDPYSLWSVAAAYWVTSDLAVRGRVENLFDEEYETAGGYKPAERSYYVSLDYQF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=636, Percent_Identity=33.6477987421384, Blast_Score=309, Evalue=3e-85,
Organism=Escherichia coli, GI1788478, Length=165, Percent_Identity=38.1818181818182, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1786798, Length=207, Percent_Identity=29.951690821256, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 68391; Mature: 68391

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTALATAVVSLLSHAYTSPVFAQNTTTETDETMVVTANRFEQSTEDVIAPMSVVTKEE
CCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHCHHH
IVQIQAKTMTDILRLQPGIEVASNGGIGQNASIFMRGTNSDHVLILIDGFRMNSSIKSGM
HHEEHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEEECCCCEEEEEEECCEECCHHHCCC
NINKIPVNQVERIEIIRGSGAVMYGSDAVGGVINIITRTSSDNQSKSATIGAGSDRYKEG
CCCCCCCCCCEEEEEEECCCEEEECCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCCC
NFAANTKVGENGHLKMAAGFTQTEGYNVNPQPGLNDGDKHGFENSQFMLNYEHQLSSYVS
CEEECCCCCCCCCEEEEECCEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHH
LFTGVRWFNSTSEYNHYTNTKSRSESESTVYSSKLSFHKDAVSSNLSISYQDDQTLEYHE
HHHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECC
NGDLDSEQKIDQLNMQWANQIKLTNDWLVNAGVDWTDAKVKADITDWSNKVIINEGESRT
CCCCCHHHHHHHHCHHHHCEEEEECCEEEECCCCCCCCEEEEEECCCCCEEEEECCCCCC
STGVYLGTQYQLEQLKLEANTRFDKHDKYDEYTTWGVGASYQINDIYKVSTAYNTAFKAP
CCEEEEECCEEEEEEEEECCCCCCCCCCCCCCEEECCCCEEEECCEEEEEEHHCHHCCCC
SFYDLSTAPDLEPETSKNIDVSLAAVYSLVNVNLSLYKNRVDNLIVYYDAPNFAGYSANV
CCEECCCCCCCCCCCCCCCCEEHHHHHHHHHCCHHHHHCCCCCEEEEECCCCCCCCCCCC
DADIKGAELELLFDTGVISHTLIAEYKDHEDTEGQQLARRAKQNYKWMGNVSLGDLDLNL
CCCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCEEEECCEEEEEEEEE
SYIYTGKRSDLPSAPGVETAYLDPYSLWSVAAAYWVTSDLAVRGRVENLFDEEYETAGGY
EEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHCCCC
KPAERSYYVSLDYQF
CCCCCEEEEEEEECC
>Mature Secondary Structure
MKKTALATAVVSLLSHAYTSPVFAQNTTTETDETMVVTANRFEQSTEDVIAPMSVVTKEE
CCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHCHHH
IVQIQAKTMTDILRLQPGIEVASNGGIGQNASIFMRGTNSDHVLILIDGFRMNSSIKSGM
HHEEHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEEECCCCEEEEEEECCEECCHHHCCC
NINKIPVNQVERIEIIRGSGAVMYGSDAVGGVINIITRTSSDNQSKSATIGAGSDRYKEG
CCCCCCCCCCEEEEEEECCCEEEECCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCCC
NFAANTKVGENGHLKMAAGFTQTEGYNVNPQPGLNDGDKHGFENSQFMLNYEHQLSSYVS
CEEECCCCCCCCCEEEEECCEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHH
LFTGVRWFNSTSEYNHYTNTKSRSESESTVYSSKLSFHKDAVSSNLSISYQDDQTLEYHE
HHHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECC
NGDLDSEQKIDQLNMQWANQIKLTNDWLVNAGVDWTDAKVKADITDWSNKVIINEGESRT
CCCCCHHHHHHHHCHHHHCEEEEECCEEEECCCCCCCCEEEEEECCCCCEEEEECCCCCC
STGVYLGTQYQLEQLKLEANTRFDKHDKYDEYTTWGVGASYQINDIYKVSTAYNTAFKAP
CCEEEEECCEEEEEEEEECCCCCCCCCCCCCCEEECCCCEEEECCEEEEEEHHCHHCCCC
SFYDLSTAPDLEPETSKNIDVSLAAVYSLVNVNLSLYKNRVDNLIVYYDAPNFAGYSANV
CCEECCCCCCCCCCCCCCCCEEHHHHHHHHHCCHHHHHCCCCCEEEEECCCCCCCCCCCC
DADIKGAELELLFDTGVISHTLIAEYKDHEDTEGQQLARRAKQNYKWMGNVSLGDLDLNL
CCCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCEEEECCEEEEEEEEE
SYIYTGKRSDLPSAPGVETAYLDPYSLWSVAAAYWVTSDLAVRGRVENLFDEEYETAGGY
EEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHCCCC
KPAERSYYVSLDYQF
CCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA