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The map label for this gene is hcaT [H]

Identifier: 59712987

GI number: 59712987

Start: 2668481

End: 2669746

Strand: Reverse

Name: hcaT [H]

Synonym: VF_2380

Alternate gene names: 59712987

Gene position: 2669746-2668481 (Counterclockwise)

Preceding gene: 59712999

Following gene: 59712986

Centisome position: 92.14

GC content: 39.1

Gene sequence:

>1266_bases
ATGAATATGAAACCAAAGTCACATTTTAAAAGGTGCATTTTTCTTCTTGTTCTATTTTCCCTTTTTATAAATCAATACGA
TGCCATAATAACCGCCTCTTTTTACTTCCTTTTGGACATCATCATGTTACGGGTTTCACCTTTTGCTTGGATCTCTCAAT
ATTTTGGCGGATTCTTTTTTGCTTATGGGGTTTATTTACCTTTTTGGGCGTTATGGTTTGAAGAACAAGGTGTTTCTGCC
GGAGATATTGGTGTTCTTATTGGGTTAGGTTTTGCAACTCGTTGTGTTGCTAATTTGGTTATTACACCAAGGTTGCATCG
CGTTGAACACATAGTACCTGCATTGCGATTATTAACCTTAGCTTCTTTGATAGCCCTTGCTCTCTTTTTAGTTAATGGGG
CGAGCTTTTGGTGGCTTGCTATCGTTACTGTTTTATTCAATTTATCTTGTGGTCCAGCGGTTCCGTTATCCGATGCGATG
ACGAACTATTACGCGAAAAATAACTTATTAGATTATGGGCGTACGCGTTTGTGGGGCTCTGTTAGTTTTATTGCAGGTTC
CACTGTTGTTGGTTATTTGGTATCGGGTTTTGGTACCGATATGATCGTCTACACGGCGATTGCCGGTATATTAGCGGCGT
TAGTGTTAGTTATGCGAAATCCTAATCCAATGCCAGTATCGGTATCAGATGAAGATAATGAACGACCAAAATTATTAGCT
CTATTAACTGAAGCTCCAATTGTGAAATTCATTGTGTTGGTGGCATTAATTCAAGGTAGTCATGCAGCGTATTACAGTTT
TAGTTCAATTTATTGGAAAGAATCTGGTTACCAAGAAAGTACCATTGGTTATTTATGGAGCTTAGGTGTTGTTGCTGAAG
TGATGGTATTTGCGTTTAGTAAGCAATTATTTGCAGGTTGGAAAACATCAACCTTATTTAAAGTTGCTGCTATTGGTGTT
GTGATTCGTTGGGGATTAACTGCTGCAACGACAGAATTATTTGCTTTGGTATTAGTGCAAGCCTTACATGGCGTGACATT
CGCAATGGCTCACCTTGCAGCAATTCAATACATTCAAAAATTCCCACAAAATAAAATGGTGGCATTACAAGCGTTATATA
ATGCGATTCCGCTTGGTGCATTTATCGCGTTAATGACAACCTTAAGTGGTTGGGGGTACGAAGCGTGGGGAGCGAATATA
TTCTGGTTGATGGCAGCGATGGGGGTATTCGCTTTGTTGATTAAACTCGATAAAGTGAAGTCTTAA

Upstream 100 bases:

>100_bases
TTAATGTCATTTGGCATGGCCCCATCCTTAGTCAGCAATTTCCCTTATTTTCATGAAAAAGTGAGCAAGAATGAAGACGA
CTTTAGTCTAGGAGATTGGT

Downstream 100 bases:

>100_bases
TCACATTTGTCAGCTTGTATAAAAACGAAGCAATAATTAAACGCTAGAATGGTGCTACAGAATAAATGCACCTTCTGGCG
TTTTTTATATCGCTAGAATG

Product: putative 3-phenylpropionic acid transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MNMKPKSHFKRCIFLLVLFSLFINQYDAIITASFYFLLDIIMLRVSPFAWISQYFGGFFFAYGVYLPFWALWFEEQGVSA
GDIGVLIGLGFATRCVANLVITPRLHRVEHIVPALRLLTLASLIALALFLVNGASFWWLAIVTVLFNLSCGPAVPLSDAM
TNYYAKNNLLDYGRTRLWGSVSFIAGSTVVGYLVSGFGTDMIVYTAIAGILAALVLVMRNPNPMPVSVSDEDNERPKLLA
LLTEAPIVKFIVLVALIQGSHAAYYSFSSIYWKESGYQESTIGYLWSLGVVAEVMVFAFSKQLFAGWKTSTLFKVAAIGV
VIRWGLTAATTELFALVLVQALHGVTFAMAHLAAIQYIQKFPQNKMVALQALYNAIPLGAFIALMTTLSGWGYEAWGANI
FWLMAAMGVFALLIKLDKVKS

Sequences:

>Translated_421_residues
MNMKPKSHFKRCIFLLVLFSLFINQYDAIITASFYFLLDIIMLRVSPFAWISQYFGGFFFAYGVYLPFWALWFEEQGVSA
GDIGVLIGLGFATRCVANLVITPRLHRVEHIVPALRLLTLASLIALALFLVNGASFWWLAIVTVLFNLSCGPAVPLSDAM
TNYYAKNNLLDYGRTRLWGSVSFIAGSTVVGYLVSGFGTDMIVYTAIAGILAALVLVMRNPNPMPVSVSDEDNERPKLLA
LLTEAPIVKFIVLVALIQGSHAAYYSFSSIYWKESGYQESTIGYLWSLGVVAEVMVFAFSKQLFAGWKTSTLFKVAAIGV
VIRWGLTAATTELFALVLVQALHGVTFAMAHLAAIQYIQKFPQNKMVALQALYNAIPLGAFIALMTTLSGWGYEAWGANI
FWLMAAMGVFALLIKLDKVKS
>Mature_421_residues
MNMKPKSHFKRCIFLLVLFSLFINQYDAIITASFYFLLDIIMLRVSPFAWISQYFGGFFFAYGVYLPFWALWFEEQGVSA
GDIGVLIGLGFATRCVANLVITPRLHRVEHIVPALRLLTLASLIALALFLVNGASFWWLAIVTVLFNLSCGPAVPLSDAM
TNYYAKNNLLDYGRTRLWGSVSFIAGSTVVGYLVSGFGTDMIVYTAIAGILAALVLVMRNPNPMPVSVSDEDNERPKLLA
LLTEAPIVKFIVLVALIQGSHAAYYSFSSIYWKESGYQESTIGYLWSLGVVAEVMVFAFSKQLFAGWKTSTLFKVAAIGV
VIRWGLTAATTELFALVLVQALHGVTFAMAHLAAIQYIQKFPQNKMVALQALYNAIPLGAFIALMTTLSGWGYEAWGANI
FWLMAAMGVFALLIKLDKVKS

Specific function: Probable permease involved in the uptake of 3- phenylpropionic acid [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family. HcaT subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788886, Length=374, Percent_Identity=38.7700534759358, Blast_Score=258, Evalue=6e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR016196
- InterPro:   IPR005276 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 46491; Mature: 46491

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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C
>Mature Secondary Structure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C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9603882; 9205837; 9278503 [H]