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The map label for this gene is gpsA

Identifier: 59712955

GI number: 59712955

Start: 2635620

End: 2636651

Strand: Reverse

Name: gpsA

Synonym: VF_2348

Alternate gene names: 59712955

Gene position: 2636651-2635620 (Counterclockwise)

Preceding gene: 59712956

Following gene: 172087765

Centisome position: 91.0

GC content: 42.73

Gene sequence:

>1032_bases
ATGGTCACAATGACAACACAAACTGATTACTCTGATATTTCAATGACCGTACTTGGTGCTGGTTCATACGGAACGTCTCT
GGCTATTTCCCTCGCTCGTAATGGCGCAAAAGTGATTTTATGGGGCCATGAAGAAGCACATATGAATCGTCTTGAAGCCG
ATCGCGCAAATGAAGAGTTTTTACCTGGTGTTGCTTTTCCACCATCATTAATCATGTCGACTGATCTTGAAAAATCAGTT
CAAGCGAGCCGTGATTTACTTGTTGTAGTTCCAAGTCATGTGTTTGGTTTGGTTCTTGGTAATGTAAAACCATTTTTACG
TGATGATTCTCGTATCTGCTGGGCAACAAAAGGGCTTGAGCCTGAAACAGGTCGTTTATTAAAAGAAGTGGCAGTTGAGG
CGTTAGGTGAATCGCATTCACTGGCTGTGCTTTCTGGACCTACTTTTGCTAAAGAACTTGCTTCTGGTATGCCAACGGCG
ATCGCTGTAGCGTCTCCTGATAAAGAGTTTGTTAAAGACCTACAAGAAAAAATCCACTGTTCTAAAACATTCCGTGTTTA
TGCAAATAATGATTTTACGGGAATGCAATTAGGTGGCGCGGTGAAAAACGTAATCGCTATTGGTGCGGGTATGTCTGATG
GTATTGGTTTTGGCGCTAATGCTCGTACGGCATTAATCACTCGTGGCTTAGCTGAAATGACTCGATTAGGTGTGGCTTTA
GGTGCTGAAGCTGAAACTTTCATGGGCATGGCAGGTTTAGGTGACTTAGTATTAACTTGTACTGATAACCAGTCTCGTAA
CCGTCGTTTTGGTTTGGCTTTAGGCCAAGGTGGCGATGTAGATAGCGCTCAAGAAGAAATTGGTCAAGTGGTTGAAGGTT
ATCGCAATACCAAAGAAGTTTGGCTGCTTGCGAATCGTATGGGCGTTGAAATGCCTATAGTTGAGCAAATTTATCAAGTA
TTGTATCAAGGAAAAGATGCAAGAGTCGCTGCGCAGGACTTATTAGCGCGAGACAAAAAATCAGAATCGTAA

Upstream 100 bases:

>100_bases
TTTTATCTAATATGGTTTCTATACGCTTTATAGTTTCTGTATTTTGGTAGAAAGTGCATTTTGCTTTTAATATAACTGTA
TCTCTTGATACTTGTTTTGG

Downstream 100 bases:

>100_bases
ATCAAGGATGGTATTAAGATGAATGAATGTAAGAAACACAAAGTATGGGAATGTATTGTTCGAGAAGCGCGTGAGCAATC
AGAACAAGAACCCATGCTTG

Product: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MVTMTTQTDYSDISMTVLGAGSYGTSLAISLARNGAKVILWGHEEAHMNRLEADRANEEFLPGVAFPPSLIMSTDLEKSV
QASRDLLVVVPSHVFGLVLGNVKPFLRDDSRICWATKGLEPETGRLLKEVAVEALGESHSLAVLSGPTFAKELASGMPTA
IAVASPDKEFVKDLQEKIHCSKTFRVYANNDFTGMQLGGAVKNVIAIGAGMSDGIGFGANARTALITRGLAEMTRLGVAL
GAEAETFMGMAGLGDLVLTCTDNQSRNRRFGLALGQGGDVDSAQEEIGQVVEGYRNTKEVWLLANRMGVEMPIVEQIYQV
LYQGKDARVAAQDLLARDKKSES

Sequences:

>Translated_343_residues
MVTMTTQTDYSDISMTVLGAGSYGTSLAISLARNGAKVILWGHEEAHMNRLEADRANEEFLPGVAFPPSLIMSTDLEKSV
QASRDLLVVVPSHVFGLVLGNVKPFLRDDSRICWATKGLEPETGRLLKEVAVEALGESHSLAVLSGPTFAKELASGMPTA
IAVASPDKEFVKDLQEKIHCSKTFRVYANNDFTGMQLGGAVKNVIAIGAGMSDGIGFGANARTALITRGLAEMTRLGVAL
GAEAETFMGMAGLGDLVLTCTDNQSRNRRFGLALGQGGDVDSAQEEIGQVVEGYRNTKEVWLLANRMGVEMPIVEQIYQV
LYQGKDARVAAQDLLARDKKSES
>Mature_343_residues
MVTMTTQTDYSDISMTVLGAGSYGTSLAISLARNGAKVILWGHEEAHMNRLEADRANEEFLPGVAFPPSLIMSTDLEKSV
QASRDLLVVVPSHVFGLVLGNVKPFLRDDSRICWATKGLEPETGRLLKEVAVEALGESHSLAVLSGPTFAKELASGMPTA
IAVASPDKEFVKDLQEKIHCSKTFRVYANNDFTGMQLGGAVKNVIAIGAGMSDGIGFGANARTALITRGLAEMTRLGVAL
GAEAETFMGMAGLGDLVLTCTDNQSRNRRFGLALGQGGDVDSAQEEIGQVVEGYRNTKEVWLLANRMGVEMPIVEQIYQV
LYQGKDARVAAQDLLARDKKSES

Specific function: De novo phospholipid biosynthesis; glycerol-3 phosphate formation. [C]

COG id: COG0240

COG function: function code C; Glycerol-3-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI33695088, Length=337, Percent_Identity=27.0029673590504, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI24307999, Length=338, Percent_Identity=25.7396449704142, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1790037, Length=331, Percent_Identity=71.2990936555891, Blast_Score=484, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI32564399, Length=341, Percent_Identity=26.099706744868, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32564403, Length=350, Percent_Identity=25.7142857142857, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI193210136, Length=342, Percent_Identity=25.7309941520468, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17507425, Length=342, Percent_Identity=23.9766081871345, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI193210134, Length=187, Percent_Identity=28.8770053475936, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320181, Length=343, Percent_Identity=28.8629737609329, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324513, Length=346, Percent_Identity=26.878612716763, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17136202, Length=335, Percent_Identity=27.7611940298507, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17136200, Length=335, Percent_Identity=27.7611940298507, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17136204, Length=335, Percent_Identity=27.7611940298507, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI22026922, Length=334, Percent_Identity=26.3473053892216, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI45551945, Length=265, Percent_Identity=26.0377358490566, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281362270, Length=265, Percent_Identity=26.0377358490566, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24648969, Length=210, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPDA_VIBF1 (Q5E2A3)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205731.1
- ProteinModelPortal:   Q5E2A3
- SMR:   Q5E2A3
- STRING:   Q5E2A3
- GeneID:   3279594
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2348
- NMPDR:   fig|312309.3.peg.2348
- eggNOG:   COG0240
- HOGENOM:   HBG586392
- OMA:   NVAKGIE
- PhylomeDB:   Q5E2A3
- ProtClustDB:   PRK00094
- BioCyc:   VFIS312309:VF2348-MONOMER
- HAMAP:   MF_00394
- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR006168
- InterPro:   IPR006109
- InterPro:   IPR011128
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR11728
- PIRSF:   PIRSF000114
- PRINTS:   PR00077

Pfam domain/function: PF07479 NAD_Gly3P_dh_C; PF01210 NAD_Gly3P_dh_N; SSF48179 6DGDH_C_like

EC number: =1.1.1.94

Molecular weight: Translated: 36755; Mature: 36755

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00957 NAD_G3PDH

Important sites: ACT_SITE 202-202 BINDING 117-117 BINDING 117-117 BINDING 150-150 BINDING 266-266 BINDING 292-292

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTMTTQTDYSDISMTVLGAGSYGTSLAISLARNGAKVILWGHEEAHMNRLEADRANEEF
CEEECCCCCCCCEEEEEEECCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHCCCCCC
LPGVAFPPSLIMSTDLEKSVQASRDLLVVVPSHVFGLVLGNVKPFLRDDSRICWATKGLE
CCCCCCCCHHHHHCCHHHHHHCCCCEEEEECHHHHHHHHCCCCHHHCCCCEEEEEECCCC
PETGRLLKEVAVEALGESHSLAVLSGPTFAKELASGMPTAIAVASPDKEFVKDLQEKIHC
CHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCC
SKTFRVYANNDFTGMQLGGAVKNVIAIGAGMSDGIGFGANARTALITRGLAEMTRLGVAL
CCEEEEEECCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GAEAETFMGMAGLGDLVLTCTDNQSRNRRFGLALGQGGDVDSAQEEIGQVVEGYRNTKEV
CCCHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHE
WLLANRMGVEMPIVEQIYQVLYQGKDARVAAQDLLARDKKSES
EEEEHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVTMTTQTDYSDISMTVLGAGSYGTSLAISLARNGAKVILWGHEEAHMNRLEADRANEEF
CEEECCCCCCCCEEEEEEECCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHCCCCCC
LPGVAFPPSLIMSTDLEKSVQASRDLLVVVPSHVFGLVLGNVKPFLRDDSRICWATKGLE
CCCCCCCCHHHHHCCHHHHHHCCCCEEEEECHHHHHHHHCCCCHHHCCCCEEEEEECCCC
PETGRLLKEVAVEALGESHSLAVLSGPTFAKELASGMPTAIAVASPDKEFVKDLQEKIHC
CHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCC
SKTFRVYANNDFTGMQLGGAVKNVIAIGAGMSDGIGFGANARTALITRGLAEMTRLGVAL
CCEEEEEECCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GAEAETFMGMAGLGDLVLTCTDNQSRNRRFGLALGQGGDVDSAQEEIGQVVEGYRNTKEV
CCCHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHE
WLLANRMGVEMPIVEQIYQVLYQGKDARVAAQDLLARDKKSES
EEEEHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA