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The map label for this gene is pfkA [H]

Identifier: 59712947

GI number: 59712947

Start: 2629060

End: 2630022

Strand: Reverse

Name: pfkA [H]

Synonym: VF_2340

Alternate gene names: 59712947

Gene position: 2630022-2629060 (Counterclockwise)

Preceding gene: 59712948

Following gene: 59712945

Centisome position: 90.77

GC content: 42.78

Gene sequence:

>963_bases
ATGGTCAAGAAGATTGGTGTTTTAACAAGTGGTGGCGATGCGCCGGGTATGAATGCGGCAGTTCGTGGTGTTGTTCGTAC
AGCATTGACGGAAGGCCTAGAAGTATTTGGTATTCATGATGGCTACTTAGGTCTTGTTGAAGATCGTATCGAAAAATTAG
AGCGTCACAGCGTATCTGATATGATCAACCGCGGCGGTACATTCTTAGGTTCAGCACGTTTTCCTGAGTTTAAAGAAGTA
GCTGTTCGTGAAAAAGCGATCGAAAACCTTAAGAAACACGACATTGATGCGCTAATCGTTATCGGTGGTGACGGTTCTTA
CATGGGTGCGAAGAAATTAACTGAAATGGGTTACCCATGTATCGGTCTTCCTGGCACAATCGATAATGATATCGCGGGTA
CTGACTACACAATTGGTTACCTAACAGCATTAAACACTGTTATTGATGCAATTGACCGTCTACGTGACACGTCTTCTTCT
CACCAACGTATTTCTATTGTAGAAGTTATGGGTCGTCACTGTGGTGATTTAACTCTTATGTCTGCAATTGCTGGTGGTTG
TGAGTACGTTATTACTCCTGAAACAGGTTTAAACAAAGAAGCTCTGATTCAAAATATCCAAGATGGTATTGCGAAAGGTA
AAAAACACGCAATCATCGCGATCACTGAGCTGATGACAGATGTTAACCAACTTGCAAAAGAAATCGAAGCTGAAACGGGT
CGTGAAACTCGTGCAACGGTTCTTGGTCACATTCAGCGTGGTGGTCAACCTGGTGCATTCGACCGTATCCTAGCTTCTCG
TATGGGTAACTATGGTGTGAAATTGCTGATTGATGGTCACGGTGGCCGTTGTGTTGGTATTCAAAATGAACAACTTGTTC
ACCACGATATCATCGATGCAATTGAAAACATGCGTCGTCCAGAAAAACTTGAGCTATACAAAGTAGCTGAAGAGTTATTC
TAA

Upstream 100 bases:

>100_bases
GAAAAAAAATTACAGATTATTAATGGTGAGCAGATTAGGGAGTTTGCTTGCTGTGTTCAAAATTGAAATTGAAATTCCCA
ACCATATTTAGAGGGTAACC

Downstream 100 bases:

>100_bases
TTTAGAATTGAATTCTAGACACAAAAAACCGAAGCGAGTGCTTCGGTTTTTTTATGCCTGTAATTATTCCAAATATAAGT
CGAGAGGTGTTTTGCTTTTT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MVKKIGVLTSGGDAPGMNAAVRGVVRTALTEGLEVFGIHDGYLGLVEDRIEKLERHSVSDMINRGGTFLGSARFPEFKEV
AVREKAIENLKKHDIDALIVIGGDGSYMGAKKLTEMGYPCIGLPGTIDNDIAGTDYTIGYLTALNTVIDAIDRLRDTSSS
HQRISIVEVMGRHCGDLTLMSAIAGGCEYVITPETGLNKEALIQNIQDGIAKGKKHAIIAITELMTDVNQLAKEIEAETG
RETRATVLGHIQRGGQPGAFDRILASRMGNYGVKLLIDGHGGRCVGIQNEQLVHHDIIDAIENMRRPEKLELYKVAEELF

Sequences:

>Translated_320_residues
MVKKIGVLTSGGDAPGMNAAVRGVVRTALTEGLEVFGIHDGYLGLVEDRIEKLERHSVSDMINRGGTFLGSARFPEFKEV
AVREKAIENLKKHDIDALIVIGGDGSYMGAKKLTEMGYPCIGLPGTIDNDIAGTDYTIGYLTALNTVIDAIDRLRDTSSS
HQRISIVEVMGRHCGDLTLMSAIAGGCEYVITPETGLNKEALIQNIQDGIAKGKKHAIIAITELMTDVNQLAKEIEAETG
RETRATVLGHIQRGGQPGAFDRILASRMGNYGVKLLIDGHGGRCVGIQNEQLVHHDIIDAIENMRRPEKLELYKVAEELF
>Mature_320_residues
MVKKIGVLTSGGDAPGMNAAVRGVVRTALTEGLEVFGIHDGYLGLVEDRIEKLERHSVSDMINRGGTFLGSARFPEFKEV
AVREKAIENLKKHDIDALIVIGGDGSYMGAKKLTEMGYPCIGLPGTIDNDIAGTDYTIGYLTALNTVIDAIDRLRDTSSS
HQRISIVEVMGRHCGDLTLMSAIAGGCEYVITPETGLNKEALIQNIQDGIAKGKKHAIIAITELMTDVNQLAKEIEAETG
RETRATVLGHIQRGGQPGAFDRILASRMGNYGVKLLIDGHGGRCVGIQNEQLVHHDIIDAIENMRRPEKLELYKVAEELF

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453768, Length=314, Percent_Identity=42.6751592356688, Blast_Score=214, Evalue=8e-56,
Organism=Homo sapiens, GI266453748, Length=314, Percent_Identity=42.6751592356688, Blast_Score=214, Evalue=8e-56,
Organism=Homo sapiens, GI4505749, Length=314, Percent_Identity=42.6751592356688, Blast_Score=214, Evalue=8e-56,
Organism=Homo sapiens, GI266453619, Length=314, Percent_Identity=42.6751592356688, Blast_Score=214, Evalue=9e-56,
Organism=Homo sapiens, GI11321601, Length=312, Percent_Identity=40.7051282051282, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI48762920, Length=311, Percent_Identity=42.1221864951768, Blast_Score=205, Evalue=4e-53,
Organism=Escherichia coli, GI1790350, Length=320, Percent_Identity=75.625, Blast_Score=503, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI25147584, Length=313, Percent_Identity=38.0191693290735, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17558788, Length=308, Percent_Identity=39.2857142857143, Blast_Score=189, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI72003601, Length=290, Percent_Identity=30.6896551724138, Blast_Score=143, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321679, Length=370, Percent_Identity=36.4864864864865, Blast_Score=199, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6323861, Length=341, Percent_Identity=33.7243401759531, Blast_Score=176, Evalue=5e-45,
Organism=Drosophila melanogaster, GI28573326, Length=302, Percent_Identity=41.0596026490066, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24652337, Length=302, Percent_Identity=40.7284768211921, Blast_Score=199, Evalue=1e-51,
Organism=Drosophila melanogaster, GI17647809, Length=302, Percent_Identity=40.7284768211921, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 34691; Mature: 34691

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKIGVLTSGGDAPGMNAAVRGVVRTALTEGLEVFGIHDGYLGLVEDRIEKLERHSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
MINRGGTFLGSARFPEFKEVAVREKAIENLKKHDIDALIVIGGDGSYMGAKKLTEMGYPC
HHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCCC
IGLPGTIDNDIAGTDYTIGYLTALNTVIDAIDRLRDTSSSHQRISIVEVMGRHCGDLTLM
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHH
SAIAGGCEYVITPETGLNKEALIQNIQDGIAKGKKHAIIAITELMTDVNQLAKEIEAETG
HHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCC
RETRATVLGHIQRGGQPGAFDRILASRMGNYGVKLLIDGHGGRCVGIQNEQLVHHDIIDA
CHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHH
IENMRRPEKLELYKVAEELF
HHHCCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MVKKIGVLTSGGDAPGMNAAVRGVVRTALTEGLEVFGIHDGYLGLVEDRIEKLERHSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
MINRGGTFLGSARFPEFKEVAVREKAIENLKKHDIDALIVIGGDGSYMGAKKLTEMGYPC
HHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCCC
IGLPGTIDNDIAGTDYTIGYLTALNTVIDAIDRLRDTSSSHQRISIVEVMGRHCGDLTLM
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHH
SAIAGGCEYVITPETGLNKEALIQNIQDGIAKGKKHAIIAITELMTDVNQLAKEIEAETG
HHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCC
RETRATVLGHIQRGGQPGAFDRILASRMGNYGVKLLIDGHGGRCVGIQNEQLVHHDIIDA
CHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHH
IENMRRPEKLELYKVAEELF
HHHCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA