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The map label for this gene is purA

Identifier: 59712925

GI number: 59712925

Start: 2605159

End: 2606475

Strand: Reverse

Name: purA

Synonym: VF_2318

Alternate gene names: 59712925

Gene position: 2606475-2605159 (Counterclockwise)

Preceding gene: 59712926

Following gene: 59712924

Centisome position: 89.95

GC content: 43.2

Gene sequence:

>1317_bases
ATGGGAAATAACGTTGTCGTTCTGGGCACCCAATGGGGTGACGAAGGTAAAGGTAAAATCGTAGACCTTTTAACTGAAGA
TGCAAAGTATGTAGTTCGCTATCAAGGCGGTCACAATGCAGGCCACACTCTAGTTATCGACGGTGAAAAAACCGTTCTAC
ACTTAATTCCATCAGGTATTTTACGTGATAATGTTGAATGCATTATCGGTAATGGCGTAGTGCTTTCACCTGATGCTTTA
CTAAAAGAAATGGCGCCACTTGAAGCGCGTGGTATTCCAGTTCGTGAACGCCTATTCATTTCTGAAGCTTGTCCTCTAAT
TCTTCCTTATCACGTAGCGCTAGACCAAGCGCGTGAAATCGCACGTGGTAACAAAGCAATTGGTACAACAGGTCGTGGTA
TCGGTCCAGCGTACGAAGATAAAGTATCTCGTCGTGGTCTACGTGTTGGCGACCTATTTGATAAAGTTGCTTTCGCTGAA
AAACTAAAAGAAGTAATGGAATTCCATAACTTCGCATTAGTTAACTACTACAAAGTAGAACCAGTAAGCTACGAAGAAGT
TCTTGAGCAAGCAATGAGCTATGCTGATCTACTAACTTCAATGGTTATCGATGTAACTGATACTCTTGATGCAGCACGTA
AGCGCGGCGACAAGATCATGTTTGAAGGTGCTCAAGGTACTCTTCTAGATATCGACCACGGTACTTACCCTTACGTAACA
TCTTCTAACACTACCGCTGGTGGCGTTGCTGCAGGTTCTGGTTTTGGTCCTCGTCACTTAGGTTACATCCTAGGTATTAC
TAAAGCATACTGTACTCGTGTAGGTTCAGGTCCATTCCCTACTGAGTTATACGATGGTTTAGAGAAGCAAGATCCAATTG
GTAAACACCTAGGCACTGTTGGCCACGAGTTTGGCGCAACAACTGGTCGTCTACGTCGTACTGGTTGGTTTGATGCTGTT
GCGATGCGTCGTGCAATCCAAATCAACTCACTATCTGGTATGTGTCTAACTAAACTAGACGTATTAGATGGCTTAGAAGA
ACTAAAAATCTGTACTGGTTACCAGATGAAAGATGGTTCTTTACTAGAAGTTTCTCCAATGGCTGCTGAAGCGTTTGAAG
AAGCAACTCCAGTATACGAAACAATGCCAGGTTGGTCTGAAAACACATTTGGTGCGAAAACATTAGAAGAGCTTCCACAA
GCTGCTCTAAACTACATCAAGCGTATTGAAGATCTAACAGGCGTGCCAATTGATATCATCTCTACAGGTCCTGACCGTAA
CGAGACAATCATCAAAGTACACCCATTCCAAGCTTAA

Upstream 100 bases:

>100_bases
TAGCGCTTTATAGTGAAAGTGAGATTTCGGTTAAAAATAACTGAAAGATTTTTCTATCAATGCTAGAATCCATTTTTAAC
TAACAAGCAGACTTGGAAAG

Downstream 100 bases:

>100_bases
TCGCTTAGTGAATGAAAGAAAAACCAGCTCATGTAGCTGGTTTTTTTATGTCTGGTATTCCAGTTTCTAGTCCCTAGTTT
CTGATCCCTAGTTTCTGATC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MGNNVVVLGTQWGDEGKGKIVDLLTEDAKYVVRYQGGHNAGHTLVIDGEKTVLHLIPSGILRDNVECIIGNGVVLSPDAL
LKEMAPLEARGIPVRERLFISEACPLILPYHVALDQAREIARGNKAIGTTGRGIGPAYEDKVSRRGLRVGDLFDKVAFAE
KLKEVMEFHNFALVNYYKVEPVSYEEVLEQAMSYADLLTSMVIDVTDTLDAARKRGDKIMFEGAQGTLLDIDHGTYPYVT
SSNTTAGGVAAGSGFGPRHLGYILGITKAYCTRVGSGPFPTELYDGLEKQDPIGKHLGTVGHEFGATTGRLRRTGWFDAV
AMRRAIQINSLSGMCLTKLDVLDGLEELKICTGYQMKDGSLLEVSPMAAEAFEEATPVYETMPGWSENTFGAKTLEELPQ
AALNYIKRIEDLTGVPIDIISTGPDRNETIIKVHPFQA

Sequences:

>Translated_438_residues
MGNNVVVLGTQWGDEGKGKIVDLLTEDAKYVVRYQGGHNAGHTLVIDGEKTVLHLIPSGILRDNVECIIGNGVVLSPDAL
LKEMAPLEARGIPVRERLFISEACPLILPYHVALDQAREIARGNKAIGTTGRGIGPAYEDKVSRRGLRVGDLFDKVAFAE
KLKEVMEFHNFALVNYYKVEPVSYEEVLEQAMSYADLLTSMVIDVTDTLDAARKRGDKIMFEGAQGTLLDIDHGTYPYVT
SSNTTAGGVAAGSGFGPRHLGYILGITKAYCTRVGSGPFPTELYDGLEKQDPIGKHLGTVGHEFGATTGRLRRTGWFDAV
AMRRAIQINSLSGMCLTKLDVLDGLEELKICTGYQMKDGSLLEVSPMAAEAFEEATPVYETMPGWSENTFGAKTLEELPQ
AALNYIKRIEDLTGVPIDIISTGPDRNETIIKVHPFQA
>Mature_437_residues
GNNVVVLGTQWGDEGKGKIVDLLTEDAKYVVRYQGGHNAGHTLVIDGEKTVLHLIPSGILRDNVECIIGNGVVLSPDALL
KEMAPLEARGIPVRERLFISEACPLILPYHVALDQAREIARGNKAIGTTGRGIGPAYEDKVSRRGLRVGDLFDKVAFAEK
LKEVMEFHNFALVNYYKVEPVSYEEVLEQAMSYADLLTSMVIDVTDTLDAARKRGDKIMFEGAQGTLLDIDHGTYPYVTS
SNTTAGGVAAGSGFGPRHLGYILGITKAYCTRVGSGPFPTELYDGLEKQDPIGKHLGTVGHEFGATTGRLRRTGWFDAVA
MRRAIQINSLSGMCLTKLDVLDGLEELKICTGYQMKDGSLLEVSPMAAEAFEEATPVYETMPGWSENTFGAKTLEELPQA
ALNYIKRIEDLTGVPIDIISTGPDRNETIIKVHPFQA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=431, Percent_Identity=42.4593967517401, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI34577063, Length=442, Percent_Identity=42.5339366515837, Blast_Score=316, Evalue=2e-86,
Organism=Homo sapiens, GI40316944, Length=402, Percent_Identity=40.2985074626866, Blast_Score=279, Evalue=4e-75,
Organism=Escherichia coli, GI1790620, Length=438, Percent_Identity=76.027397260274, Blast_Score=683, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25146555, Length=440, Percent_Identity=42.7272727272727, Blast_Score=321, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI25146553, Length=440, Percent_Identity=42.7272727272727, Blast_Score=321, Evalue=6e-88,
Organism=Saccharomyces cerevisiae, GI6324109, Length=438, Percent_Identity=40.8675799086758, Blast_Score=321, Evalue=2e-88,
Organism=Drosophila melanogaster, GI21356033, Length=431, Percent_Identity=41.5313225058005, Blast_Score=327, Evalue=8e-90,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_VIBF1 (Q5E2D3)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205701.1
- ProteinModelPortal:   Q5E2D3
- SMR:   Q5E2D3
- STRING:   Q5E2D3
- GeneID:   3279443
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2318
- NMPDR:   fig|312309.3.peg.2318
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- PhylomeDB:   Q5E2D3
- ProtClustDB:   PRK01117
- BioCyc:   VFIS312309:VF2318-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47693; Mature: 47562

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 14-14 ACT_SITE 42-42 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 310-310 BINDING 312-312

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNNVVVLGTQWGDEGKGKIVDLLTEDAKYVVRYQGGHNAGHTLVIDGEKTVLHLIPSGI
CCCCEEEEEECCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEEECCCCEEEEECCCHH
LRDNVECIIGNGVVLSPDALLKEMAPLEARGIPVRERLFISEACPLILPYHVALDQAREI
HHCCCEEEEECCEEECHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHH
ARGNKAIGTTGRGIGPAYEDKVSRRGLRVGDLFDKVAFAEKLKEVMEFHNFALVNYYKVE
HCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEC
PVSYEEVLEQAMSYADLLTSMVIDVTDTLDAARKRGDKIMFEGAQGTLLDIDHGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVAAGSGFGPRHLGYILGITKAYCTRVGSGPFPTELYDGLEKQDPIGKHLGTV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHH
GHEFGATTGRLRRTGWFDAVAMRRAIQINSLSGMCLTKLDVLDGLEELKICTGYQMKDGS
HHHHCCCCCCHHHCCHHHHHHHHHHHEECCCCCHHHHHHHHHCCHHHHHHHCCCEECCCC
LLEVSPMAAEAFEEATPVYETMPGWSENTFGAKTLEELPQAALNYIKRIEDLTGVPIDII
EEEECHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
STGPDRNETIIKVHPFQA
ECCCCCCCEEEEEECCCC
>Mature Secondary Structure 
GNNVVVLGTQWGDEGKGKIVDLLTEDAKYVVRYQGGHNAGHTLVIDGEKTVLHLIPSGI
CCCEEEEEECCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEEECCCCEEEEECCCHH
LRDNVECIIGNGVVLSPDALLKEMAPLEARGIPVRERLFISEACPLILPYHVALDQAREI
HHCCCEEEEECCEEECHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHH
ARGNKAIGTTGRGIGPAYEDKVSRRGLRVGDLFDKVAFAEKLKEVMEFHNFALVNYYKVE
HCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEC
PVSYEEVLEQAMSYADLLTSMVIDVTDTLDAARKRGDKIMFEGAQGTLLDIDHGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVAAGSGFGPRHLGYILGITKAYCTRVGSGPFPTELYDGLEKQDPIGKHLGTV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHH
GHEFGATTGRLRRTGWFDAVAMRRAIQINSLSGMCLTKLDVLDGLEELKICTGYQMKDGS
HHHHCCCCCCHHHCCHHHHHHHHHHHEECCCCCHHHHHHHHHCCHHHHHHHCCCEECCCC
LLEVSPMAAEAFEEATPVYETMPGWSENTFGAKTLEELPQAALNYIKRIEDLTGVPIDII
EEEECHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
STGPDRNETIIKVHPFQA
ECCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA