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The map label for this gene is hmp [H]

Identifier: 59712923

GI number: 59712923

Start: 2602983

End: 2604167

Strand: Direct

Name: hmp [H]

Synonym: VF_2316

Alternate gene names: 59712923

Gene position: 2602983-2604167 (Clockwise)

Preceding gene: 59712915

Following gene: 59712935

Centisome position: 89.83

GC content: 38.73

Gene sequence:

>1185_bases
ATGCTCAACAAAAACACCATTGATATCGTTAAATCAACAATTCCATTACTTGCTGAAACTGGTCCTGCTTTAACTGCGCA
TTTTTACGATCGCATGTTTACACATAACCCCGAGTTAAAAGATATTTTTAACATGAGCAATCAACGTAATGGCGACCAAC
GTGAAGCATTATTTAATGCTATTTGCGCCTATGCAGGGAATATTGAAAATCTTGAAGTGCTATTACCAGCGGTTGAAAAG
ATTGCTCATAAGCATACGAGCTTTATGATCACAGCTGAGCAGTACAATATTGTTGGTGGTCACTTACTTGCCACTCTTGA
TGAAATGTTTTCCCCTGGACAAGAAGTGTTAGATGCTTGGGGTGAAGCTTATGGCGTTCTCGCAAATGTATTTATTCAAC
GTGAAGAACAAATATATCAAGAGAATAACAACCAAGAAGGCGGCTGGCGTGGTCTACGTGAGTTTGAGCTTATTGCAAAA
GAAAAAGAGAGTGAGCTAATCACAAGCTTTACCTTTATACCTACAGATGACCAACCTGTCTCTCGCTTTAAACCAGGTCA
ATATTTAGGGATTTACTTAACTCCTGAAGAATTTGAAAACCAAGAAATTCGTCAGTACAGTTTATCTTCTGCCCCACAAG
AAAAAACATATCGTATATCAGTAAAACGAGAAGATGGCGGTAAAGCATCAAACTATTTACATGACCAATTGAATATTGGT
GATAAGGTCAATCTTGCTGCCCCTGCTGGTGACTTTTTCTTAGATGTTGAGGCATCAACACCAGTAACACTTATTTCGGC
TGGAGTTGGATTAACACCAACCCTTTCTATGCTTGAAACATTAACAGAGCATAACGCAAACGTGAGCTGGTTACATGCTA
CAGAAAATGGTACTCAACATGCCTTTAGAGAACATGTAAATACGTTAGCAGAAGCCCACTCACATATTTCGACTTACACT
TGGTATAACCAACCGCAAGAAGTAGATAAGCTAAATCAACATTTTGACTTCTCAGGTTTTATGAATTTATCTGAAGTTAA
AGATGAAATCATTACAGACAATGGCCACTACTATTTCTGTGGACCGGTTGGCTTTATGCAACACGTCGCAAAACAATTAG
TTGAGCTAGGTGTTTCCGAAGACCGTATCCATTACGAATGCTTTGGACCACATAAAGTGCTTTAA

Upstream 100 bases:

>100_bases
TCCTTCTCTTTTTTCATTGTAATTCTTCTCTTGACTAAAAGATATATTTTAGATACAACTTATAACATACATTATAAATA
CACCTTTAAAGGTAAACAAT

Downstream 100 bases:

>100_bases
TTTTTATAAAGTAGAAATGCAAAAACGCCCAATGACATAATCATTGGGCGTTTTTTCGAGTTGATGAAACTCAATCGACT
AAAATGAATCTTCTTTCAGG

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MLNKNTIDIVKSTIPLLAETGPALTAHFYDRMFTHNPELKDIFNMSNQRNGDQREALFNAICAYAGNIENLEVLLPAVEK
IAHKHTSFMITAEQYNIVGGHLLATLDEMFSPGQEVLDAWGEAYGVLANVFIQREEQIYQENNNQEGGWRGLREFELIAK
EKESELITSFTFIPTDDQPVSRFKPGQYLGIYLTPEEFENQEIRQYSLSSAPQEKTYRISVKREDGGKASNYLHDQLNIG
DKVNLAAPAGDFFLDVEASTPVTLISAGVGLTPTLSMLETLTEHNANVSWLHATENGTQHAFREHVNTLAEAHSHISTYT
WYNQPQEVDKLNQHFDFSGFMNLSEVKDEIITDNGHYYFCGPVGFMQHVAKQLVELGVSEDRIHYECFGPHKVL

Sequences:

>Translated_394_residues
MLNKNTIDIVKSTIPLLAETGPALTAHFYDRMFTHNPELKDIFNMSNQRNGDQREALFNAICAYAGNIENLEVLLPAVEK
IAHKHTSFMITAEQYNIVGGHLLATLDEMFSPGQEVLDAWGEAYGVLANVFIQREEQIYQENNNQEGGWRGLREFELIAK
EKESELITSFTFIPTDDQPVSRFKPGQYLGIYLTPEEFENQEIRQYSLSSAPQEKTYRISVKREDGGKASNYLHDQLNIG
DKVNLAAPAGDFFLDVEASTPVTLISAGVGLTPTLSMLETLTEHNANVSWLHATENGTQHAFREHVNTLAEAHSHISTYT
WYNQPQEVDKLNQHFDFSGFMNLSEVKDEIITDNGHYYFCGPVGFMQHVAKQLVELGVSEDRIHYECFGPHKVL
>Mature_394_residues
MLNKNTIDIVKSTIPLLAETGPALTAHFYDRMFTHNPELKDIFNMSNQRNGDQREALFNAICAYAGNIENLEVLLPAVEK
IAHKHTSFMITAEQYNIVGGHLLATLDEMFSPGQEVLDAWGEAYGVLANVFIQREEQIYQENNNQEGGWRGLREFELIAK
EKESELITSFTFIPTDDQPVSRFKPGQYLGIYLTPEEFENQEIRQYSLSSAPQEKTYRISVKREDGGKASNYLHDQLNIG
DKVNLAAPAGDFFLDVEASTPVTLISAGVGLTPTLSMLETLTEHNANVSWLHATENGTQHAFREHVNTLAEAHSHISTYT
WYNQPQEVDKLNQHFDFSGFMNLSEVKDEIITDNGHYYFCGPVGFMQHVAKQLVELGVSEDRIHYECFGPHKVL

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=396, Percent_Identity=64.6464646464647, Blast_Score=540, Evalue=1e-155,
Organism=Escherichia coli, GI1788104, Length=241, Percent_Identity=26.1410788381743, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1787658, Length=241, Percent_Identity=24.896265560166, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321673, Length=414, Percent_Identity=35.9903381642512, Blast_Score=229, Evalue=5e-61,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 44536; Mature: 44536

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNKNTIDIVKSTIPLLAETGPALTAHFYDRMFTHNPELKDIFNMSNQRNGDQREALFNA
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHH
ICAYAGNIENLEVLLPAVEKIAHKHTSFMITAEQYNIVGGHLLATLDEMFSPGQEVLDAW
HHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHH
GEAYGVLANVFIQREEQIYQENNNQEGGWRGLREFELIAKEKESELITSFTFIPTDDQPV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCH
SRFKPGQYLGIYLTPEEFENQEIRQYSLSSAPQEKTYRISVKREDGGKASNYLHDQLNIG
HHCCCCCEEEEEECHHHHCCHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCC
DKVNLAAPAGDFFLDVEASTPVTLISAGVGLTPTLSMLETLTEHNANVSWLHATENGTQH
CCEEEECCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHH
AFREHVNTLAEAHSHISTYTWYNQPQEVDKLNQHFDFSGFMNLSEVKDEIITDNGHYYFC
HHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHCCCCHHCCCHHHHHHHHCCCCCCEEEE
GPVGFMQHVAKQLVELGVSEDRIHYECFGPHKVL
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MLNKNTIDIVKSTIPLLAETGPALTAHFYDRMFTHNPELKDIFNMSNQRNGDQREALFNA
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHH
ICAYAGNIENLEVLLPAVEKIAHKHTSFMITAEQYNIVGGHLLATLDEMFSPGQEVLDAW
HHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHH
GEAYGVLANVFIQREEQIYQENNNQEGGWRGLREFELIAKEKESELITSFTFIPTDDQPV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCH
SRFKPGQYLGIYLTPEEFENQEIRQYSLSSAPQEKTYRISVKREDGGKASNYLHDQLNIG
HHCCCCCEEEEEECHHHHCCHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCC
DKVNLAAPAGDFFLDVEASTPVTLISAGVGLTPTLSMLETLTEHNANVSWLHATENGTQH
CCEEEECCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHH
AFREHVNTLAEAHSHISTYTWYNQPQEVDKLNQHFDFSGFMNLSEVKDEIITDNGHYYFC
HHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHCCCCHHCCCHHHHHHHHCCCCCCEEEE
GPVGFMQHVAKQLVELGVSEDRIHYECFGPHKVL
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA