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The map label for this gene is pilQ [H]

Identifier: 59712900

GI number: 59712900

Start: 2575651

End: 2577399

Strand: Reverse

Name: pilQ [H]

Synonym: VF_2293

Alternate gene names: 59712900

Gene position: 2577399-2575651 (Counterclockwise)

Preceding gene: 59712901

Following gene: 59712899

Centisome position: 88.95

GC content: 39.22

Gene sequence:

>1749_bases
ATGTCGCTAGGACGCAATAAACTCATCAATTCATATTGTATTCGGTTATGTGCATTTATTATGTTCAGTTTCGTTCATTT
GAATGCCATTGCTGAAGAGTCAGTGATGAATCAACTTGTTGAAGTAGATTTTCAACTAAGTAAGAAAAAAGAAGGTGTCA
TAACAGTAGAGCTAGCTTCTGCTGCTGCGACGGTGGATTTACGTAAAGAAAAAGATAAGTTGATTATTGAATTGCATGAC
ACCAAAGTCAGCGATGATAAATTGGTTATCTTAGATGTTGAAGATTTTGCCACTCTTGTTTCTCGTATTGAAACATTTAG
AGAGCCAAGTAGTACCGTTTTATCTGTTGATGTTAAAGGTGACTATCAATATGACTACTTCCTGAAAGGACCTTCCTTAC
AAGTGGTGGTATCTCAATTGCCAGAGAGTGAGAAAAAATCGCCGATTAGCTCCAATAATGAAGATAAAAGTAAGCCTATT
TCGATTAACTTCCAAGATATTCCAGTTCGTCATGTATTGCAGCTGATCGCGGATTATAATGATTTTAACTTGGTTGTTGC
TGACTCTGTTACGGGGAATTTAACCTTACGTCTGGATGGTGTGCCATGGCAGCAAGTGCTTGATATTATTCTTCAAGTTA
AAGGGTTAGATAAACGTGTCGAGGGTAATGTTGTTTTGATCGCACCCAAAACAGAGCTTGATGCACAAGAGCAGCAAGTA
TTAGAGCAAGCTCAAATGGCATCTGAGCTGGCTGCGCTATCATCTGAGATCCTTTCTATTAAATACGCTAAAGCGTCAGA
TATTGCAGAGCTGCTTGTTGGTGAAGGTGAAATTACCATGTTATCTAGCCGTGGCAGTATCACGGTAGATGAGCGTACAA
ACTCTTTATTGTTAAGAGATCTTCCAGAGAACATTACGGTAATTAAAGACATCATTGATACGTTAGATATTCCAATTAAA
CAGGTTCAAATTGAAGCTCGTATTGTAACGGTCAATGAAGGTAACTTAGATGAGTTAGGGGTTCGTTGGGGGATAACGAA
TACCAATGGCAGTACAACGGTTGGTGGTTCTATAGAGAGCAATTTAGCCGCTGTGGGGTTGTATGATGGTGGTGGAAGTG
ATGATAAGAAGAGTGGCAATAATAGTCTTCCTGTCGATGATTTCTTAAATGTGAATCTAGGATTAGCGAATCCTGCCGCT
TCAAGTATTGCTTTTCAGGTAGCGAAATTAGGCTCTGATTTATTGCTTGATTTAGAACTTTCGGCACTTCAAGCTGAATC
TAAGGCTGAGATTATCTCTAGTCCACGTTTGATTACCACCAATAAGAAACCTGCGTATATTGAACAAGGTACAGAGATCC
CTTACTTAGAGGCTTCATCCAGTGGGGCAACATCCGTTTCATTTAAAAAAGCGGTATTAAGCTTAATGGTGACGCCACAA
ATTACGCCTGATAATCGATTAGTTTTAGACTTAACGGTAACACAAGATAGACCGGGGCAAGTGGTTAAAACAGGTACCGG
TGAAGCCGTAGCGATTGATACACAACGTATTGGAACCCAAGTTTTGGTAGATAATGGGGAAACGGTTGTTTTAGGTGGTA
TTTATCAACACTCGTTATCAAATAGCGTTGAGAAAGTGCCAGTTCTTGGTGACTTACCGTTACTTGGAGCGTTATTTCGT
CGAAGCTCTGAAAATATAGGTAAAAAAGAATTATTGATATTCGTTACGCCAAAAGTTGTTATACAGTAA

Upstream 100 bases:

>100_bases
CGTTGAAAGAAACCCTGCCAGATGGAATGGGATGCTGGCAACAAAGATATGTAAAACTGGCGTTGAAACATTAATTGAAA
TAAAGGATTAGGAATCTCTC

Downstream 100 bases:

>100_bases
TGAGATAGTATTCAAATTCAGCATGATTTATGGAAAAAAAAGCGGTAAAAAAAAATAATTATGCTGAACTTTTCATCTAG
CTATTGCAATCACTACTAGT

Product: pili ecretion protein PilQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 582; Mature: 581

Protein sequence:

>582_residues
MSLGRNKLINSYCIRLCAFIMFSFVHLNAIAEESVMNQLVEVDFQLSKKKEGVITVELASAAATVDLRKEKDKLIIELHD
TKVSDDKLVILDVEDFATLVSRIETFREPSSTVLSVDVKGDYQYDYFLKGPSLQVVVSQLPESEKKSPISSNNEDKSKPI
SINFQDIPVRHVLQLIADYNDFNLVVADSVTGNLTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPKTELDAQEQQV
LEQAQMASELAALSSEILSIKYAKASDIAELLVGEGEITMLSSRGSITVDERTNSLLLRDLPENITVIKDIIDTLDIPIK
QVQIEARIVTVNEGNLDELGVRWGITNTNGSTTVGGSIESNLAAVGLYDGGGSDDKKSGNNSLPVDDFLNVNLGLANPAA
SSIAFQVAKLGSDLLLDLELSALQAESKAEIISSPRLITTNKKPAYIEQGTEIPYLEASSSGATSVSFKKAVLSLMVTPQ
ITPDNRLVLDLTVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVDNGETVVLGGIYQHSLSNSVEKVPVLGDLPLLGALFR
RSSENIGKKELLIFVTPKVVIQ

Sequences:

>Translated_582_residues
MSLGRNKLINSYCIRLCAFIMFSFVHLNAIAEESVMNQLVEVDFQLSKKKEGVITVELASAAATVDLRKEKDKLIIELHD
TKVSDDKLVILDVEDFATLVSRIETFREPSSTVLSVDVKGDYQYDYFLKGPSLQVVVSQLPESEKKSPISSNNEDKSKPI
SINFQDIPVRHVLQLIADYNDFNLVVADSVTGNLTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPKTELDAQEQQV
LEQAQMASELAALSSEILSIKYAKASDIAELLVGEGEITMLSSRGSITVDERTNSLLLRDLPENITVIKDIIDTLDIPIK
QVQIEARIVTVNEGNLDELGVRWGITNTNGSTTVGGSIESNLAAVGLYDGGGSDDKKSGNNSLPVDDFLNVNLGLANPAA
SSIAFQVAKLGSDLLLDLELSALQAESKAEIISSPRLITTNKKPAYIEQGTEIPYLEASSSGATSVSFKKAVLSLMVTPQ
ITPDNRLVLDLTVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVDNGETVVLGGIYQHSLSNSVEKVPVLGDLPLLGALFR
RSSENIGKKELLIFVTPKVVIQ
>Mature_581_residues
SLGRNKLINSYCIRLCAFIMFSFVHLNAIAEESVMNQLVEVDFQLSKKKEGVITVELASAAATVDLRKEKDKLIIELHDT
KVSDDKLVILDVEDFATLVSRIETFREPSSTVLSVDVKGDYQYDYFLKGPSLQVVVSQLPESEKKSPISSNNEDKSKPIS
INFQDIPVRHVLQLIADYNDFNLVVADSVTGNLTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPKTELDAQEQQVL
EQAQMASELAALSSEILSIKYAKASDIAELLVGEGEITMLSSRGSITVDERTNSLLLRDLPENITVIKDIIDTLDIPIKQ
VQIEARIVTVNEGNLDELGVRWGITNTNGSTTVGGSIESNLAAVGLYDGGGSDDKKSGNNSLPVDDFLNVNLGLANPAAS
SIAFQVAKLGSDLLLDLELSALQAESKAEIISSPRLITTNKKPAYIEQGTEIPYLEASSSGATSVSFKKAVLSLMVTPQI
TPDNRLVLDLTVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVDNGETVVLGGIYQHSLSNSVEKVPVLGDLPLLGALFRR
SSENIGKKELLIFVTPKVVIQ

Specific function: Essential for the formation of pili. Involved in the biogenesis of type 4 fimbriae probably by serving as a "porthole" allowing passage of the fimbrae through the outer membrane [H]

COG id: COG4796

COG function: function code U; Type II secretory pathway, component HofQ

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI1789793, Length=427, Percent_Identity=37.7049180327869, Blast_Score=271, Evalue=1e-73,
Organism=Escherichia coli, GI87082242, Length=347, Percent_Identity=29.971181556196, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR013355
- InterPro:   IPR011662
- InterPro:   IPR004846
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N; PF07660 STN [H]

EC number: NA

Molecular weight: Translated: 63136; Mature: 63005

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLGRNKLINSYCIRLCAFIMFSFVHLNAIAEESVMNQLVEVDFQLSKKKEGVITVELAS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCEEEEEECC
AAATVDLRKEKDKLIIELHDTKVSDDKLVILDVEDFATLVSRIETFREPSSTVLSVDVKG
CEEEEEEECCCCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCEEEEEEECC
DYQYDYFLKGPSLQVVVSQLPESEKKSPISSNNEDKSKPISINFQDIPVRHVLQLIADYN
CEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCC
DFNLVVADSVTGNLTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPKTELDAQEQQV
CCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCEECCCCEEEEECCCCCCHHHHHH
LEQAQMASELAALSSEILSIKYAKASDIAELLVGEGEITMLSSRGSITVDERTNSLLLRD
HHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHCCCCCEEEEECCCCEEEECCCCCEEEEC
LPENITVIKDIIDTLDIPIKQVQIEARIVTVNEGNLDELGVRWGITNTNGSTTVGGSIES
CCCCHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCHHCEEEEEEECCCCCEEECCCCCC
NLAAVGLYDGGGSDDKKSGNNSLPVDDFLNVNLGLANPAASSIAFQVAKLGSDLLLDLEL
CEEEEEEECCCCCCCCCCCCCCCCHHHHEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEH
SALQAESKAEIISSPRLITTNKKPAYIEQGTEIPYLEASSSGATSVSFKKAVLSLMVTPQ
HHHCCCHHHHHHCCCEEEECCCCCCEEECCCCCCEEECCCCCCCCHHHHHHHHHHEECCC
ITPDNRLVLDLTVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVDNGETVVLGGIYQHSLS
CCCCCEEEEEEEECCCCCCCEEEECCCCEEEEEHHHCCEEEEEECCCEEEEECHHHHHHC
NSVEKVPVLGDLPLLGALFRRSSENIGKKELLIFVTPKVVIQ
CCHHCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCEEEEC
>Mature Secondary Structure 
SLGRNKLINSYCIRLCAFIMFSFVHLNAIAEESVMNQLVEVDFQLSKKKEGVITVELAS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCEEEEEECC
AAATVDLRKEKDKLIIELHDTKVSDDKLVILDVEDFATLVSRIETFREPSSTVLSVDVKG
CEEEEEEECCCCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCEEEEEEECC
DYQYDYFLKGPSLQVVVSQLPESEKKSPISSNNEDKSKPISINFQDIPVRHVLQLIADYN
CEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCC
DFNLVVADSVTGNLTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPKTELDAQEQQV
CCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCEECCCCEEEEECCCCCCHHHHHH
LEQAQMASELAALSSEILSIKYAKASDIAELLVGEGEITMLSSRGSITVDERTNSLLLRD
HHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHCCCCCEEEEECCCCEEEECCCCCEEEEC
LPENITVIKDIIDTLDIPIKQVQIEARIVTVNEGNLDELGVRWGITNTNGSTTVGGSIES
CCCCHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCHHCEEEEEEECCCCCEEECCCCCC
NLAAVGLYDGGGSDDKKSGNNSLPVDDFLNVNLGLANPAASSIAFQVAKLGSDLLLDLEL
CEEEEEEECCCCCCCCCCCCCCCCHHHHEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEH
SALQAESKAEIISSPRLITTNKKPAYIEQGTEIPYLEASSSGATSVSFKKAVLSLMVTPQ
HHHCCCHHHHHHCCCEEEECCCCCCEEECCCCCCEEECCCCCCCCHHHHHHHHHHEECCC
ITPDNRLVLDLTVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVDNGETVVLGGIYQHSLS
CCCCCEEEEEEEECCCCCCCEEEECCCCEEEEEHHHCCEEEEEECCCEEEEECHHHHHHC
NSVEKVPVLGDLPLLGALFRRSSENIGKKELLIFVTPKVVIQ
CCHHCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7901733; 10984043 [H]