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The map label for this gene is dam [H]

Identifier: 59712896

GI number: 59712896

Start: 2571338

End: 2572168

Strand: Reverse

Name: dam [H]

Synonym: VF_2289

Alternate gene names: 59712896

Gene position: 2572168-2571338 (Counterclockwise)

Preceding gene: 59712897

Following gene: 59712895

Centisome position: 88.77

GC content: 37.79

Gene sequence:

>831_bases
ATGAAAAAGCATCGCGCATTTCTCAAGTGGGCTGGCGGTAAATATAGCCTAGTAGAAGAAATTCAACGTCATCTCCCTGA
TGCTAGAAAATTGATCGAGCCTTTTGTTGGGGCTGGTTCTGTATTTTTGAATACAGATTATGACCATTATCTATTAGCGG
ATATTAATCCAGACCTTATCAATCTTTATAATCACCTTAAAGATGAACCCGAGCGTTTTATTGAAGACGCTCGAGCGTTA
TTTCAACCAGAATACAATAAAAAAGAAGTGTATTTAGCGTTACGTGTTGAGTTTAATCAGTGTACTGATACTTATCGTCG
TTCGTTATTATTTTTATATATGAACCGCCATGGCTTTAATGGTTTATGCCGTTATAACAAAAAAGGTGGCTTTAATGTAC
CATTTGGTTCGTATAAAAAACCGTATTTCCCAGAGAAAGAAATGGTGTTTTTTGCTGAAAAAGCGAAAAAAGCGACGTTT
GTTTGTGAAGGGTATATGGATACTTTTAAACGTGCTCGTAAAGGTGCGGTTGTTTATTGTGATCCTCCTTATGCACCATT
ATCAACGACGGCTAATTTTACCTCTTATGCAGGAAATGGTTTTTCGTTGGATGATCAAGCGGCATTAGCTGATATTGCTG
AGAAAGCCGCATTTGAGCGTGATATTCCAGTATTGATTTCAAATCACGACACAACACTGACTCGACGCTTATATCATGGT
GCGACACTTAATACGATTAAAGTGAAAAGAACCATTAGTCGAAATGGTGCTGGGCGAAATAAAGTGGATGAGCTTATGGC
TTTGTTTCATGCACCTAAATGCACAGAATAA

Upstream 100 bases:

>100_bases
ATTGATTCTGTAAAATAACCATAAGATAAACAGATCCTAGCTAAAAAATAGGGTACAATCCCCCGCTTTCTGGATTAAGG
TAAAAAGGCGTAAAAGACGT

Downstream 100 bases:

>100_bases
GTTAACTTAAGACTCTTTTTTCTTCGAACGCCAGATTTATGTGATTTGTGTCCCTTTATTCACATAAGACAGCCCCCAAA
ATATCAGGTAGAATTGCGCC

Product: DNA adenine methylase

Products: NA

Alternate protein names: DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase; M.VchADam [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKKHRAFLKWAGGKYSLVEEIQRHLPDARKLIEPFVGAGSVFLNTDYDHYLLADINPDLINLYNHLKDEPERFIEDARAL
FQPEYNKKEVYLALRVEFNQCTDTYRRSLLFLYMNRHGFNGLCRYNKKGGFNVPFGSYKKPYFPEKEMVFFAEKAKKATF
VCEGYMDTFKRARKGAVVYCDPPYAPLSTTANFTSYAGNGFSLDDQAALADIAEKAAFERDIPVLISNHDTTLTRRLYHG
ATLNTIKVKRTISRNGAGRNKVDELMALFHAPKCTE

Sequences:

>Translated_276_residues
MKKHRAFLKWAGGKYSLVEEIQRHLPDARKLIEPFVGAGSVFLNTDYDHYLLADINPDLINLYNHLKDEPERFIEDARAL
FQPEYNKKEVYLALRVEFNQCTDTYRRSLLFLYMNRHGFNGLCRYNKKGGFNVPFGSYKKPYFPEKEMVFFAEKAKKATF
VCEGYMDTFKRARKGAVVYCDPPYAPLSTTANFTSYAGNGFSLDDQAALADIAEKAAFERDIPVLISNHDTTLTRRLYHG
ATLNTIKVKRTISRNGAGRNKVDELMALFHAPKCTE
>Mature_276_residues
MKKHRAFLKWAGGKYSLVEEIQRHLPDARKLIEPFVGAGSVFLNTDYDHYLLADINPDLINLYNHLKDEPERFIEDARAL
FQPEYNKKEVYLALRVEFNQCTDTYRRSLLFLYMNRHGFNGLCRYNKKGGFNVPFGSYKKPYFPEKEMVFFAEKAKKATF
VCEGYMDTFKRARKGAVVYCDPPYAPLSTTANFTSYAGNGFSLDDQAALADIAEKAAFERDIPVLISNHDTTLTRRLYHG
ATLNTIKVKRTISRNGAGRNKVDELMALFHAPKCTE

Specific function: Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=269, Percent_Identity=62.8252788104089, Blast_Score=353, Evalue=9e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31701; Mature: 31701

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHRAFLKWAGGKYSLVEEIQRHLPDARKLIEPFVGAGSVFLNTDYDHYLLADINPDLI
CCCCCCCEEECCCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCEEEEECCHHHH
NLYNHLKDEPERFIEDARALFQPEYNKKEVYLALRVEFNQCTDTYRRSLLFLYMNRHGFN
HHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEECHHHHHHHHHHEEEEEECCCCCC
GLCRYNKKGGFNVPFGSYKKPYFPEKEMVFFAEKAKKATFVCEGYMDTFKRARKGAVVYC
CEEEECCCCCCCCCCCCCCCCCCCCHHEEEEEHHCCCCEEEEHHHHHHHHHHHCCCEEEE
DPPYAPLSTTANFTSYAGNGFSLDDQAALADIAEKAAFERDIPVLISNHDTTLTRRLYHG
CCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCC
ATLNTIKVKRTISRNGAGRNKVDELMALFHAPKCTE
CCEEEEEEEEEHHCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKHRAFLKWAGGKYSLVEEIQRHLPDARKLIEPFVGAGSVFLNTDYDHYLLADINPDLI
CCCCCCCEEECCCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCEEEEECCHHHH
NLYNHLKDEPERFIEDARALFQPEYNKKEVYLALRVEFNQCTDTYRRSLLFLYMNRHGFN
HHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEECHHHHHHHHHHEEEEEECCCCCC
GLCRYNKKGGFNVPFGSYKKPYFPEKEMVFFAEKAKKATFVCEGYMDTFKRARKGAVVYC
CEEEECCCCCCCCCCCCCCCCCCCCHHEEEEEHHCCCCEEEEHHHHHHHHHHHCCCEEEE
DPPYAPLSTTANFTSYAGNGFSLDDQAALADIAEKAAFERDIPVLISNHDTTLTRRLYHG
CCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCC
ATLNTIKVKRTISRNGAGRNKVDELMALFHAPKCTE
CCEEEEEEEEEHHCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11705940 [H]