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The map label for this gene is metJ [H]

Identifier: 59712875

GI number: 59712875

Start: 2548321

End: 2548641

Strand: Direct

Name: metJ [H]

Synonym: VF_2268

Alternate gene names: 59712875

Gene position: 2548321-2548641 (Clockwise)

Preceding gene: 59712866

Following gene: 59712876

Centisome position: 87.95

GC content: 41.74

Gene sequence:

>321_bases
ATGGCTGATTGGAATGGTGACTATTTAAGTCCTTATGCCGAACATGGTAAAAAAAGCGAACAAGTAAAAAAAATTACTGT
TTCTATTCCAACAAAGGTACTTCAAATCCTTACTGACGAGCGTACTCGTCGCCAGGTAAGCAACCTACGTCATGCGACAA
ACAGTGAATTATTGTGTGAAGCATTCCTACACGCATACACTGGCCAGCCTTTACCAACAGATGAAGATCTACGTAAAGAT
CGCCCAGATGATATTCCAGCTGAAGCAAAAGCATTAATGACGGCAATGGGGATCGAATTCGAAGCGTTTGACGAAGAGTA
A

Upstream 100 bases:

>100_bases
ACACTTCTAGACGTCTATATGTCTTTACTTTTAGCGGTAAATATCGCTAAAATCGCCTATTCTACTTATATACATCAATA
ATTCTGAACGAAGGTGAACA

Downstream 100 bases:

>100_bases
ATACGTTTCATTCCTCCTCCTTTTGTCTCCTCCTTCTTTTAATAAAATTAGGAGGATTACATTCCCTCTTGGTTAATAAA
TAATCACAGTGCCCTACTTA

Product: transcriptional repressor protein MetJ

Products: NA

Alternate protein names: Met regulon regulatory protein metJ [H]

Number of amino acids: Translated: 106; Mature: 105

Protein sequence:

>106_residues
MADWNGDYLSPYAEHGKKSEQVKKITVSIPTKVLQILTDERTRRQVSNLRHATNSELLCEAFLHAYTGQPLPTDEDLRKD
RPDDIPAEAKALMTAMGIEFEAFDEE

Sequences:

>Translated_106_residues
MADWNGDYLSPYAEHGKKSEQVKKITVSIPTKVLQILTDERTRRQVSNLRHATNSELLCEAFLHAYTGQPLPTDEDLRKD
RPDDIPAEAKALMTAMGIEFEAFDEE
>Mature_105_residues
ADWNGDYLSPYAEHGKKSEQVKKITVSIPTKVLQILTDERTRRQVSNLRHATNSELLCEAFLHAYTGQPLPTDEDLRKDR
PDDIPAEAKALMTAMGIEFEAFDEE

Specific function: This regulatory protein, when combined with SAM (S- adenosylmethionine) represses the expression of the methionine regulon and of enzymes involved in SAM synthesis [H]

COG id: COG3060

COG function: function code KE; Transcriptional regulator of met regulon

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metJ family [H]

Homologues:

Organism=Escherichia coli, GI1790373, Length=104, Percent_Identity=76.9230769230769, Blast_Score=172, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002084
- InterPro:   IPR010985
- ProDom:   PD020365 [H]

Pfam domain/function: PF01340 MetJ [H]

EC number: NA

Molecular weight: Translated: 12049; Mature: 11917

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADWNGDYLSPYAEHGKKSEQVKKITVSIPTKVLQILTDERTRRQVSNLRHATNSELLCE
CCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHH
AFLHAYTGQPLPTDEDLRKDRPDDIPAEAKALMTAMGIEFEAFDEE
HHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ADWNGDYLSPYAEHGKKSEQVKKITVSIPTKVLQILTDERTRRQVSNLRHATNSELLCE
CCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHH
AFLHAYTGQPLPTDEDLRKDRPDDIPAEAKALMTAMGIEFEAFDEE
HHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA