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The map label for this gene is ilvI [H]

Identifier: 59712870

GI number: 59712870

Start: 2539012

End: 2540733

Strand: Reverse

Name: ilvI [H]

Synonym: VF_2263

Alternate gene names: 59712870

Gene position: 2540733-2539012 (Counterclockwise)

Preceding gene: 59712871

Following gene: 59712869

Centisome position: 87.69

GC content: 41.35

Gene sequence:

>1722_bases
ATGGAAATGTTATCTGGTTCAGAGATGATCGTACAATCTCTGATTGATGAAGGTGTTGAACACATATTTGGCTACCCTGG
TGGCTCTGTGTTGGATATATATGATGCGTTGCACATGAAAAGTGATGTGATTCAGCATGTACTTGTAAGACATGAGCAAG
CAGCTACGCATATGGCTGATGGCTATGCAAGAGCAACAGGAAAGCCAGGTGTCGTTCTTGTGTGTTCTGGTCCTGGTGCT
ACCAATACCATTACGGGTATCGCTACCGCTTATATGGACTCAATACCAATGGTTGTTCTTTCTGGTAACGTTCCTACTAA
TATGATCGGTAACGATGCTTTCCAAGAGTGTGACATGGTTGGAGTCTCTCGACCTGTAGTCAAGCACAGCTTCTTAGTTA
AGAAAGCCGAAGATATCCCTGAAACCCTTAAAAAAGCCTTTTATATTGCTTCTACTGGCCGTCCAGGTCCCGTGGTTATT
GATATCCCGAAAGATGTAATGAACCCTGCTATTAAGTTTTCATATCAATACCCTGAATCCATGAAGATGCGTTCGTATAA
TCCAACAACATCTGGTCATAAAGGACAGATTAAAAAAGGATTAAAAACATTATTAGAGGCTAAGAAGCCTGTTTTGTATG
TTGGCGGTGGCGCGATAATTTCTTCTGCAGAAAAACAAATACTAAAATTAGCTGAGACTCTTAATTTACCTGTTGTAAGT
ACCTTGATGGGATTGGGAGCTTTTCCTGGCACTCATAAAAATGCACTTGGCATGTTAGGTATGCATGGTAAGTATGAAGC
TAATATGGCTATGCATAACGCAGATCTTATTTTTGGTGTTGGGGTTCGTTTTGATGATAGAACCACCAATAACTTAGAAA
AATACTGCCCGAACGCCACGATCATGCATATCGACATTGATCCCTCGTCGATTTCAAAGAATGTTCGAGTTGATGTTCCA
ATTGTTGGCTCTGCGGAGATTGTTCTTGATTCTATGCTTAAGCTGTTAGTGGAGCAGGGAGGAACAAACGATGAGGCAGC
CTTAGCAACATGGTGGGAAGAAATTGGAGTTTGGCAAGAGCGTAATTGCTTGGCTTATGAAACCTCTCCTGATCGTATTA
AACCACAGCAGGTTATCGAAACGCTTCATAAATTAACCAATGGTGATGCTTATGTCGCTTCCGATGTTGGACAGCATCAG
ATGTTTGCTGCTTTGTATTACCCATTTAATAAGCCACGTCGTTGGATTAATTCTGGTGGACTAGGAACCATGGGGTTCGG
TTTACCAGCAGCTCTAGGGGTTAAATTTACTCATCCTGATGAAGTGGTGGTTTGTGTGACTGGTGATGGCAGTATTCAGA
TGAATATTCAGGAGCTATCTACCGCATTGCAATACGATATCCCTGTGAAAATCATTAATTTGAATAACCGTTTCTTAGGA
ATGGTGAAGCAGTGGCAAGATATTATTTACCAAGGTCGCCATTCCAATTCTTATATGAGTTCAGTTCCTGATTTTGCCGC
AATTGCTGAGGCATATGGTCATGTAGGAATTCGTATTAATCACCCTGATGAGCTGGAGTCAGGTCTAGAGAAGGCTCTTT
CTCTAAAAGATCGCCTTGTGTTTGTGGATATTAATGTTGATGAAACAGAGCACGTTTACCCAATGCAAATTAAAGGGGAA
GCAATGGATAAAATGTGGCTAAGTAAAACGGAGAGAACCTAA

Upstream 100 bases:

>100_bases
TTTCAGTTTAGGCTAAAAATAAGCCCTAACTATGTAAAACCGTTAATTTAGCGATAATTCACGTCGACTGCTTTAAGGTT
TTCAGTGTCTGGAGAACGAT

Downstream 100 bases:

>100_bases
TGAGACATATTATTTCACTATTATTAGAGAATGAACCGGGTTCTTTATCACGTGTTGTCGGCCTATTTTCTCAGCGTGGA
TACAACATTGAATCTTTAAC

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MEMLSGSEMIVQSLIDEGVEHIFGYPGGSVLDIYDALHMKSDVIQHVLVRHEQAATHMADGYARATGKPGVVLVCSGPGA
TNTITGIATAYMDSIPMVVLSGNVPTNMIGNDAFQECDMVGVSRPVVKHSFLVKKAEDIPETLKKAFYIASTGRPGPVVI
DIPKDVMNPAIKFSYQYPESMKMRSYNPTTSGHKGQIKKGLKTLLEAKKPVLYVGGGAIISSAEKQILKLAETLNLPVVS
TLMGLGAFPGTHKNALGMLGMHGKYEANMAMHNADLIFGVGVRFDDRTTNNLEKYCPNATIMHIDIDPSSISKNVRVDVP
IVGSAEIVLDSMLKLLVEQGGTNDEAALATWWEEIGVWQERNCLAYETSPDRIKPQQVIETLHKLTNGDAYVASDVGQHQ
MFAALYYPFNKPRRWINSGGLGTMGFGLPAALGVKFTHPDEVVVCVTGDGSIQMNIQELSTALQYDIPVKIINLNNRFLG
MVKQWQDIIYQGRHSNSYMSSVPDFAAIAEAYGHVGIRINHPDELESGLEKALSLKDRLVFVDINVDETEHVYPMQIKGE
AMDKMWLSKTERT

Sequences:

>Translated_573_residues
MEMLSGSEMIVQSLIDEGVEHIFGYPGGSVLDIYDALHMKSDVIQHVLVRHEQAATHMADGYARATGKPGVVLVCSGPGA
TNTITGIATAYMDSIPMVVLSGNVPTNMIGNDAFQECDMVGVSRPVVKHSFLVKKAEDIPETLKKAFYIASTGRPGPVVI
DIPKDVMNPAIKFSYQYPESMKMRSYNPTTSGHKGQIKKGLKTLLEAKKPVLYVGGGAIISSAEKQILKLAETLNLPVVS
TLMGLGAFPGTHKNALGMLGMHGKYEANMAMHNADLIFGVGVRFDDRTTNNLEKYCPNATIMHIDIDPSSISKNVRVDVP
IVGSAEIVLDSMLKLLVEQGGTNDEAALATWWEEIGVWQERNCLAYETSPDRIKPQQVIETLHKLTNGDAYVASDVGQHQ
MFAALYYPFNKPRRWINSGGLGTMGFGLPAALGVKFTHPDEVVVCVTGDGSIQMNIQELSTALQYDIPVKIINLNNRFLG
MVKQWQDIIYQGRHSNSYMSSVPDFAAIAEAYGHVGIRINHPDELESGLEKALSLKDRLVFVDINVDETEHVYPMQIKGE
AMDKMWLSKTERT
>Mature_573_residues
MEMLSGSEMIVQSLIDEGVEHIFGYPGGSVLDIYDALHMKSDVIQHVLVRHEQAATHMADGYARATGKPGVVLVCSGPGA
TNTITGIATAYMDSIPMVVLSGNVPTNMIGNDAFQECDMVGVSRPVVKHSFLVKKAEDIPETLKKAFYIASTGRPGPVVI
DIPKDVMNPAIKFSYQYPESMKMRSYNPTTSGHKGQIKKGLKTLLEAKKPVLYVGGGAIISSAEKQILKLAETLNLPVVS
TLMGLGAFPGTHKNALGMLGMHGKYEANMAMHNADLIFGVGVRFDDRTTNNLEKYCPNATIMHIDIDPSSISKNVRVDVP
IVGSAEIVLDSMLKLLVEQGGTNDEAALATWWEEIGVWQERNCLAYETSPDRIKPQQVIETLHKLTNGDAYVASDVGQHQ
MFAALYYPFNKPRRWINSGGLGTMGFGLPAALGVKFTHPDEVVVCVTGDGSIQMNIQELSTALQYDIPVKIINLNNRFLG
MVKQWQDIIYQGRHSNSYMSSVPDFAAIAEAYGHVGIRINHPDELESGLEKALSLKDRLVFVDINVDETEHVYPMQIKGE
AMDKMWLSKTERT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=603, Percent_Identity=26.865671641791, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI21361361, Length=510, Percent_Identity=25.4901960784314, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI87081685, Length=575, Percent_Identity=72.1739130434783, Blast_Score=889, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=563, Percent_Identity=40.4973357015986, Blast_Score=428, Evalue=1e-121,
Organism=Escherichia coli, GI1786717, Length=535, Percent_Identity=34.2056074766355, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1787096, Length=555, Percent_Identity=29.1891891891892, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788716, Length=576, Percent_Identity=24.1319444444444, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17531299, Length=553, Percent_Identity=26.5822784810127, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17531301, Length=553, Percent_Identity=26.5822784810127, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17542570, Length=569, Percent_Identity=25.1318101933216, Blast_Score=142, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6323755, Length=582, Percent_Identity=41.5807560137457, Blast_Score=442, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6320816, Length=547, Percent_Identity=24.6800731261426, Blast_Score=133, Evalue=9e-32,
Organism=Drosophila melanogaster, GI19922626, Length=602, Percent_Identity=24.4186046511628, Blast_Score=182, Evalue=6e-46,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62815; Mature: 62815

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMLSGSEMIVQSLIDEGVEHIFGYPGGSVLDIYDALHMKSDVIQHVLVRHEQAATHMAD
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYARATGKPGVVLVCSGPGATNTITGIATAYMDSIPMVVLSGNVPTNMIGNDAFQECDMV
CHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHC
GVSRPVVKHSFLVKKAEDIPETLKKAFYIASTGRPGPVVIDIPKDVMNPAIKFSYQYPES
CCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCHHHCCCEEEEEECCCCC
MKMRSYNPTTSGHKGQIKKGLKTLLEAKKPVLYVGGGAIISSAEKQILKLAETLNLPVVS
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHH
TLMGLGAFPGTHKNALGMLGMHGKYEANMAMHNADLIFGVGVRFDDRTTNNLEKYCPNAT
HHHHCCCCCCCCCCCHHCCCCCCCEECCEEEECCCEEEEECEEECCCCCCCHHHHCCCCE
IMHIDIDPSSISKNVRVDVPIVGSAEIVLDSMLKLLVEQGGTNDEAALATWWEEIGVWQE
EEEEECCHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCC
RNCLAYETSPDRIKPQQVIETLHKLTNGDAYVASDVGQHQMFAALYYPFNKPRRWINSGG
CCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHEEEEEECCCCCCHHHHCCCC
LGTMGFGLPAALGVKFTHPDEVVVCVTGDGSIQMNIQELSTALQYDIPVKIINLNNRFLG
CCCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHEECCCEEEEECCCHHHH
MVKQWQDIIYQGRHSNSYMSSVPDFAAIAEAYGHVGIRINHPDELESGLEKALSLKDRLV
HHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHCCEEE
FVDINVDETEHVYPMQIKGEAMDKMWLSKTERT
EEEECCCCCCCEEEEEECCCHHHHHHHHHCCCC
>Mature Secondary Structure
MEMLSGSEMIVQSLIDEGVEHIFGYPGGSVLDIYDALHMKSDVIQHVLVRHEQAATHMAD
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYARATGKPGVVLVCSGPGATNTITGIATAYMDSIPMVVLSGNVPTNMIGNDAFQECDMV
CHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHC
GVSRPVVKHSFLVKKAEDIPETLKKAFYIASTGRPGPVVIDIPKDVMNPAIKFSYQYPES
CCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCHHHCCCEEEEEECCCCC
MKMRSYNPTTSGHKGQIKKGLKTLLEAKKPVLYVGGGAIISSAEKQILKLAETLNLPVVS
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHH
TLMGLGAFPGTHKNALGMLGMHGKYEANMAMHNADLIFGVGVRFDDRTTNNLEKYCPNAT
HHHHCCCCCCCCCCCHHCCCCCCCEECCEEEECCCEEEEECEEECCCCCCCHHHHCCCCE
IMHIDIDPSSISKNVRVDVPIVGSAEIVLDSMLKLLVEQGGTNDEAALATWWEEIGVWQE
EEEEECCHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCC
RNCLAYETSPDRIKPQQVIETLHKLTNGDAYVASDVGQHQMFAALYYPFNKPRRWINSGG
CCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHEEEEEECCCCCCHHHHCCCC
LGTMGFGLPAALGVKFTHPDEVVVCVTGDGSIQMNIQELSTALQYDIPVKIINLNNRFLG
CCCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHEECCCEEEEECCCHHHH
MVKQWQDIIYQGRHSNSYMSSVPDFAAIAEAYGHVGIRINHPDELESGLEKALSLKDRLV
HHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHCCEEE
FVDINVDETEHVYPMQIKGEAMDKMWLSKTERT
EEEECCCCCCCEEEEEECCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]