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The map label for this gene is cca

Identifier: 59712851

GI number: 59712851

Start: 2520537

End: 2521760

Strand: Direct

Name: cca

Synonym: VF_2244

Alternate gene names: 59712851

Gene position: 2520537-2521760 (Clockwise)

Preceding gene: 59712848

Following gene: 59712855

Centisome position: 86.99

GC content: 42.32

Gene sequence:

>1224_bases
GTGAAAATTTATTTAGTTGGCGGTGCAGTTCGAGATTCACTTCTCAATATTGATGTAAAAGACAAAGACTGGGTTGTTGT
TGGTTCGACTCCACAGAAAATGGATTCTCTCGGTTATCAAACCGTTGGACAAGATTTCCCTGTGTTTCTGAACCCGAAAA
CTAAAGAAGAATACGCACTCGCCCGAACGGAACGAAAAAGCGGTCAAGGTTATAAAGGATTTACTTGCTATGCAGAGCCA
GATGTCACATTAGAAGAAGATTTATTACGCCGAGACCTAACAATTAATGCCATCGCTCAAGCTGATAATGGTGAGCTCAT
CGACCCTTATAATGGCCAACAAGACATTATAGACCGCACACTTCGCCATGTATCTGATGCATTTACTGAAGATCCCTTGC
GAGTTCTGCGTGTCGCTCGTTTTGCTGCTCGTTTTCATCATTTAGGCTTTACGGTTGCCCCTGAAACCATGCATCTAATG
AAAGTATTAGTGGATAGTGGTGAACTCTCTCATTTAACAGCTGAGCGAGTGTGGCAAGAGTGGCAAAAATCATTATCTAG
CCAGCACCCTGAAATCTTTCTTTCTACGCTAAAAGAGTGTGGTGCTCTTGCTATTGTGCTACCTGAACTCAATGCTTTAT
TTGGTATACCACAACCAGAAAAGTGGCACCCTGAAATTGATACGGGAATTCACACCCTAATGGTCGCTCAGCAAGCAGCT
CTATTAAGCCAAGACTTACCGACTCGCTTTGCAGCGCAAGTGCATGACCTTGGGAAAGGTGTCACGCCAGAATCAGAATG
GCCAAGCCATAAACTGCATTGCCATACAGGGATAAAACTGATTAAAAGATTGTGTGATCGTGTTCGTGTACCAAATGACT
ATCGAGATCTTGCTCTATTAGTATGTGAGCATCACTCTAATATCCACAGAGCAGCAGAATTACGAGCACAAACCTTCATT
AAAATATTCGATAAAATGGATGTGTGGCGTAAACCAGAGCGCTTAGCTCCTATACTACTTTGCTGCCAAGCTGATCATGC
AGGAAGATTGGGGTTAGAAACTCAACCTTACCCACAAAAAAAACGTTTTGAAGCGGCATTTGATGCTGCAAAAAATGTGG
AAGTGAAAGATGTGGTTGCAGCAGGATTTAAAGGACCTGAGATCAGAGAAGAGTTAAGCAAAAGACGAATTGAAGCGGTG
AAAGATAAGTTGAATATCAAATAA

Upstream 100 bases:

>100_bases
GCATGGGAATAAAAAGTCGAAAAACTCTATTTGCACGGTATCATTGGATGTAAGAAAGTAAAGAAAGTTCTCTTTATCTT
GGCAGCGATTAGGACAAAAC

Downstream 100 bases:

>100_bases
GCTATACGCACGCTAACTAGAACGCTTCGCTCTAGAAACTATAAAAGCCAAGGTTTCAGGTTTCAGGTTTCAGGTTTCAG
GTTTCAGGTTTCAGGTTTCA

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MKIYLVGGAVRDSLLNIDVKDKDWVVVGSTPQKMDSLGYQTVGQDFPVFLNPKTKEEYALARTERKSGQGYKGFTCYAEP
DVTLEEDLLRRDLTINAIAQADNGELIDPYNGQQDIIDRTLRHVSDAFTEDPLRVLRVARFAARFHHLGFTVAPETMHLM
KVLVDSGELSHLTAERVWQEWQKSLSSQHPEIFLSTLKECGALAIVLPELNALFGIPQPEKWHPEIDTGIHTLMVAQQAA
LLSQDLPTRFAAQVHDLGKGVTPESEWPSHKLHCHTGIKLIKRLCDRVRVPNDYRDLALLVCEHHSNIHRAAELRAQTFI
KIFDKMDVWRKPERLAPILLCCQADHAGRLGLETQPYPQKKRFEAAFDAAKNVEVKDVVAAGFKGPEIREELSKRRIEAV
KDKLNIK

Sequences:

>Translated_407_residues
MKIYLVGGAVRDSLLNIDVKDKDWVVVGSTPQKMDSLGYQTVGQDFPVFLNPKTKEEYALARTERKSGQGYKGFTCYAEP
DVTLEEDLLRRDLTINAIAQADNGELIDPYNGQQDIIDRTLRHVSDAFTEDPLRVLRVARFAARFHHLGFTVAPETMHLM
KVLVDSGELSHLTAERVWQEWQKSLSSQHPEIFLSTLKECGALAIVLPELNALFGIPQPEKWHPEIDTGIHTLMVAQQAA
LLSQDLPTRFAAQVHDLGKGVTPESEWPSHKLHCHTGIKLIKRLCDRVRVPNDYRDLALLVCEHHSNIHRAAELRAQTFI
KIFDKMDVWRKPERLAPILLCCQADHAGRLGLETQPYPQKKRFEAAFDAAKNVEVKDVVAAGFKGPEIREELSKRRIEAV
KDKLNIK
>Mature_407_residues
MKIYLVGGAVRDSLLNIDVKDKDWVVVGSTPQKMDSLGYQTVGQDFPVFLNPKTKEEYALARTERKSGQGYKGFTCYAEP
DVTLEEDLLRRDLTINAIAQADNGELIDPYNGQQDIIDRTLRHVSDAFTEDPLRVLRVARFAARFHHLGFTVAPETMHLM
KVLVDSGELSHLTAERVWQEWQKSLSSQHPEIFLSTLKECGALAIVLPELNALFGIPQPEKWHPEIDTGIHTLMVAQQAA
LLSQDLPTRFAAQVHDLGKGVTPESEWPSHKLHCHTGIKLIKRLCDRVRVPNDYRDLALLVCEHHSNIHRAAELRAQTFI
KIFDKMDVWRKPERLAPILLCCQADHAGRLGLETQPYPQKKRFEAAFDAAKNVEVKDVVAAGFKGPEIREELSKRRIEAV
KDKLNIK

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=199, Percent_Identity=28.643216080402, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1789436, Length=400, Percent_Identity=61.5, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI87081691, Length=264, Percent_Identity=28.7878787878788, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_VIBF1 (Q5E2K7)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205627.1
- ProteinModelPortal:   Q5E2K7
- STRING:   Q5E2K7
- GeneID:   3278424
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2244
- NMPDR:   fig|312309.3.peg.2243
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   GHGQKGL
- PhylomeDB:   Q5E2K7
- ProtClustDB:   PRK10885
- BioCyc:   VFIS312309:VF2244-MONOMER
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 46085; Mature: 46085

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYLVGGAVRDSLLNIDVKDKDWVVVGSTPQKMDSLGYQTVGQDFPVFLNPKTKEEYAL
CEEEEECCHHHHHHEECEECCCCEEEECCCHHHHHHCCHHHCCCCCCEEECCCCHHHHHH
ARTERKSGQGYKGFTCYAEPDVTLEEDLLRRDLTINAIAQADNGELIDPYNGQQDIIDRT
HHHHHHCCCCCCCEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCEECCCCCHHHHHHHH
LRHVSDAFTEDPLRVLRVARFAARFHHLGFTVAPETMHLMKVLVDSGELSHLTAERVWQE
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHCCCCHHHHHHHHHHHH
WQKSLSSQHPEIFLSTLKECGALAIVLPELNALFGIPQPEKWHPEIDTGIHTLMVAQQAA
HHHHHHCCCHHHHHHHHHHCCCEEEEECCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
LLSQDLPTRFAAQVHDLGKGVTPESEWPSHKLHCHTGIKLIKRLCDRVRVPNDYRDLALL
HHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHH
VCEHHSNIHRAAELRAQTFIKIFDKMDVWRKPERLAPILLCCQADHAGRLGLETQPYPQK
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEECCCCCCCCCCCCCCCCHH
KRFEAAFDAAKNVEVKDVVAAGFKGPEIREELSKRRIEAVKDKLNIK
HHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIYLVGGAVRDSLLNIDVKDKDWVVVGSTPQKMDSLGYQTVGQDFPVFLNPKTKEEYAL
CEEEEECCHHHHHHEECEECCCCEEEECCCHHHHHHCCHHHCCCCCCEEECCCCHHHHHH
ARTERKSGQGYKGFTCYAEPDVTLEEDLLRRDLTINAIAQADNGELIDPYNGQQDIIDRT
HHHHHHCCCCCCCEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCEECCCCCHHHHHHHH
LRHVSDAFTEDPLRVLRVARFAARFHHLGFTVAPETMHLMKVLVDSGELSHLTAERVWQE
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHCCCCHHHHHHHHHHHH
WQKSLSSQHPEIFLSTLKECGALAIVLPELNALFGIPQPEKWHPEIDTGIHTLMVAQQAA
HHHHHHCCCHHHHHHHHHHCCCEEEEECCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
LLSQDLPTRFAAQVHDLGKGVTPESEWPSHKLHCHTGIKLIKRLCDRVRVPNDYRDLALL
HHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHH
VCEHHSNIHRAAELRAQTFIKIFDKMDVWRKPERLAPILLCCQADHAGRLGLETQPYPQK
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEECCCCCCCCCCCCCCCCHH
KRFEAAFDAAKNVEVKDVVAAGFKGPEIREELSKRRIEAVKDKLNIK
HHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA