Click here to switch to the map view.

The map label for this gene is pitA [C]

Identifier: 59712847

GI number: 59712847

Start: 2516041

End: 2517321

Strand: Direct

Name: pitA [C]

Synonym: VF_2240

Alternate gene names: 59712847

Gene position: 2516041-2517321 (Clockwise)

Preceding gene: 59712846

Following gene: 59712848

Centisome position: 86.83

GC content: 43.79

Gene sequence:

>1281_bases
ATGGATATTCTTGCTAACTACGGCACGACCCTTATTTTAATCGCAGCCTTCTTTGGCTTCCTTATGGCTATTGGTATTGG
TGCAAATGATGTTGCCAATGCAATGGGCACATCTGTAGGGTCTAAAGCACTAACAGTAAAACAAGCAATCATCATTGCTA
TGATCTTCGAATTTGCAGGGGCATACCTTGCAGGTGGTGAGGTTACAGATACAATCCGCAAAGGGGTAATTGATACCTCT
CTATTCGCTAGCCAGCCTGAAGTGTTAGTTTACGGTATGAGGTCAGCACTAGTTGCTGCTGGTACTTGGCTATTACTTGC
TTCTTACATGGGCTGGCCAGTATCAACAACTCACTCAATCATTGGTGCTATCATTGGTTTCGCGTGTGTTGCTGTTGGTA
CTGAAGCGGTTGATTGGAGCTCAGTTCAAGGTATCGTTGGTAGTTGGTTAATTACCCCGCTTATCTCTGGTATCTTTGCT
TATATGATATTTATCAGTGCTCAACGACTGATATTCGATACAGATAAGCCACTGATGAATGCGAAACGTTTTGTTCCTGT
TTACATGTTCATTACGACTATGGTTATTGCTCTTGTTACGATCAAAAAAGGTCTAAAACATGTTGGCTTACATCTAAGTA
ATGGTGAGGCATGGGTTGCTTCTGCAGGTATTTCTGCATTAGTAATGGTTGGTGGTTACATGTATATTTCTCGTAAGTTT
GCAAACACGGTAAACGATGAGAAAGACAGCCGTGGTTTTACTGGCGTAGAAAGTATCTTCAGTACACTTATGGTTATCAC
TGCGTGTGCGATGGCTTTCGCTCACGGTTCAAACGATGTTGCTAATGCGATCGGTCCTCTATCTGCTGTTGTTTCAACAG
TTGAACACATGGGCGAAATCACAGCGAAAAGTACAATTGCTTGGTGGATCCTTCCTCTAGGTGGTATTGGTATCGTTGTA
GGTCTTGCAACATTAGGTCACAAAGTGATGGCAACAGTTGGTACTGGTATTACAGAACTGACTCCTAGCCGTGGTTTTGC
TGCTCAACTAGCAACAGCCTCAACGGTTGTATTAGCATCAGGTACTGGTCTTCCAATCTCTACAACCCAAACACTTGTTG
GTGCGGTTCTAGGTGTTGGTTTTGCTCGTGGTATTGGCGCACTAAACTTAGGTGTTGTTCGTAACATTGTGGCATCTTGG
GTTGTTACCCTGCCTGCTGGTGCGTTATTAGCCGTTGTTTTCTACTACGGAATGCAAGCGCTCTTCGGTATCGGCGCATA
A

Upstream 100 bases:

>100_bases
GTGGGTTGGCGGCATTGCCGACCAAGCACAACGTGTTGGTTCTCGTTTAGAACTAATGCTATCTCGCTCATAAGTTTGTG
ATTCAGTAACAGGTAATACA

Downstream 100 bases:

>100_bases
GCGTAATCAATTTTGCAACAATGGCACAAAGAGGAGAGTAATATCTCCTCTTTTCTTGCATCTGGGCCTAATTATTTTTA
GTATCTATACCTTCGTTTGA

Product: phosphate transporter, low-affinity

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MDILANYGTTLILIAAFFGFLMAIGIGANDVANAMGTSVGSKALTVKQAIIIAMIFEFAGAYLAGGEVTDTIRKGVIDTS
LFASQPEVLVYGMRSALVAAGTWLLLASYMGWPVSTTHSIIGAIIGFACVAVGTEAVDWSSVQGIVGSWLITPLISGIFA
YMIFISAQRLIFDTDKPLMNAKRFVPVYMFITTMVIALVTIKKGLKHVGLHLSNGEAWVASAGISALVMVGGYMYISRKF
ANTVNDEKDSRGFTGVESIFSTLMVITACAMAFAHGSNDVANAIGPLSAVVSTVEHMGEITAKSTIAWWILPLGGIGIVV
GLATLGHKVMATVGTGITELTPSRGFAAQLATASTVVLASGTGLPISTTQTLVGAVLGVGFARGIGALNLGVVRNIVASW
VVTLPAGALLAVVFYYGMQALFGIGA

Sequences:

>Translated_426_residues
MDILANYGTTLILIAAFFGFLMAIGIGANDVANAMGTSVGSKALTVKQAIIIAMIFEFAGAYLAGGEVTDTIRKGVIDTS
LFASQPEVLVYGMRSALVAAGTWLLLASYMGWPVSTTHSIIGAIIGFACVAVGTEAVDWSSVQGIVGSWLITPLISGIFA
YMIFISAQRLIFDTDKPLMNAKRFVPVYMFITTMVIALVTIKKGLKHVGLHLSNGEAWVASAGISALVMVGGYMYISRKF
ANTVNDEKDSRGFTGVESIFSTLMVITACAMAFAHGSNDVANAIGPLSAVVSTVEHMGEITAKSTIAWWILPLGGIGIVV
GLATLGHKVMATVGTGITELTPSRGFAAQLATASTVVLASGTGLPISTTQTLVGAVLGVGFARGIGALNLGVVRNIVASW
VVTLPAGALLAVVFYYGMQALFGIGA
>Mature_426_residues
MDILANYGTTLILIAAFFGFLMAIGIGANDVANAMGTSVGSKALTVKQAIIIAMIFEFAGAYLAGGEVTDTIRKGVIDTS
LFASQPEVLVYGMRSALVAAGTWLLLASYMGWPVSTTHSIIGAIIGFACVAVGTEAVDWSSVQGIVGSWLITPLISGIFA
YMIFISAQRLIFDTDKPLMNAKRFVPVYMFITTMVIALVTIKKGLKHVGLHLSNGEAWVASAGISALVMVGGYMYISRKF
ANTVNDEKDSRGFTGVESIFSTLMVITACAMAFAHGSNDVANAIGPLSAVVSTVEHMGEITAKSTIAWWILPLGGIGIVV
GLATLGHKVMATVGTGITELTPSRGFAAQLATASTVVLASGTGLPISTTQTLVGAVLGVGFARGIGALNLGVVRNIVASW
VVTLPAGALLAVVFYYGMQALFGIGA

Specific function: Potential transporter for phosphate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family [H]

Homologues:

Organism=Homo sapiens, GI5803173, Length=191, Percent_Identity=40.8376963350785, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI31543630, Length=191, Percent_Identity=41.8848167539267, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25146401, Length=461, Percent_Identity=36.0086767895879, Blast_Score=257, Evalue=9e-69,
Organism=Caenorhabditis elegans, GI71981576, Length=443, Percent_Identity=35.8916478555305, Blast_Score=250, Evalue=8e-67,
Organism=Caenorhabditis elegans, GI17536725, Length=497, Percent_Identity=26.7605633802817, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17539280, Length=208, Percent_Identity=42.7884615384615, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI32566716, Length=236, Percent_Identity=36.0169491525424, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17557328, Length=507, Percent_Identity=26.0355029585799, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6319773, Length=179, Percent_Identity=40.7821229050279, Blast_Score=122, Evalue=8e-29,
Organism=Drosophila melanogaster, GI21356511, Length=259, Percent_Identity=37.4517374517375, Blast_Score=172, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 44150; Mature: 44150

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDILANYGTTLILIAAFFGFLMAIGIGANDVANAMGTSVGSKALTVKQAIIIAMIFEFAG
CCCHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
AYLAGGEVTDTIRKGVIDTSLFASQPEVLVYGMRSALVAAGTWLLLASYMGWPVSTTHSI
CHHCCCHHHHHHHHCCHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
IGAIIGFACVAVGTEAVDWSSVQGIVGSWLITPLISGIFAYMIFISAQRLIFDTDKPLMN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHH
AKRFVPVYMFITTMVIALVTIKKGLKHVGLHLSNGEAWVASAGISALVMVGGYMYISRKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHH
ANTVNDEKDSRGFTGVESIFSTLMVITACAMAFAHGSNDVANAIGPLSAVVSTVEHMGEI
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
TAKSTIAWWILPLGGIGIVVGLATLGHKVMATVGTGITELTPSRGFAAQLATASTVVLAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHEEEEEC
GTGLPISTTQTLVGAVLGVGFARGIGALNLGVVRNIVASWVVTLPAGALLAVVFYYGMQA
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LFGIGA
HHHCCC
>Mature Secondary Structure
MDILANYGTTLILIAAFFGFLMAIGIGANDVANAMGTSVGSKALTVKQAIIIAMIFEFAG
CCCHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
AYLAGGEVTDTIRKGVIDTSLFASQPEVLVYGMRSALVAAGTWLLLASYMGWPVSTTHSI
CHHCCCHHHHHHHHCCHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
IGAIIGFACVAVGTEAVDWSSVQGIVGSWLITPLISGIFAYMIFISAQRLIFDTDKPLMN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHH
AKRFVPVYMFITTMVIALVTIKKGLKHVGLHLSNGEAWVASAGISALVMVGGYMYISRKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHH
ANTVNDEKDSRGFTGVESIFSTLMVITACAMAFAHGSNDVANAIGPLSAVVSTVEHMGEI
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
TAKSTIAWWILPLGGIGIVVGLATLGHKVMATVGTGITELTPSRGFAAQLATASTVVLAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHEEEEEC
GTGLPISTTQTLVGAVLGVGFARGIGALNLGVVRNIVASWVVTLPAGALLAVVFYYGMQA
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LFGIGA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]