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The map label for this gene is parE [H]

Identifier: 59712835

GI number: 59712835

Start: 2500279

End: 2502159

Strand: Reverse

Name: parE [H]

Synonym: VF_2228

Alternate gene names: 59712835

Gene position: 2502159-2500279 (Counterclockwise)

Preceding gene: 59712836

Following gene: 59712834

Centisome position: 86.35

GC content: 42.42

Gene sequence:

>1881_bases
ATGACTGAACAATATAATGCAGGAGCCATTGAGGTTCTAAATGGCCTTGAGCCCGTTCGTCGCCGTCCTGGTATGTACAC
CGACACTGTTCGTCCAAACCATTTGGGCCAAGAAGTTATTGATAACAGTGTCGATGAAGCCCTAGCCGGACACGCGAAAA
AAGTTGAAGTAATTTTACATGCCGATCAATCACTTGAAGTTATTGATGATGGGCGTGGTATGCCTGTTGATATCCACCCA
GTAGAAAAAGTTTCTGGTGTTGAACTTATTTTATGTAAGTTGCATGCAGGTGGTAAATTCTCAGGTAAAAACTACGAATT
CTCAGGTGGCTTACACGGGGTTGGTATCTCTGTAGTAAATGCTTTGTCAAAACGAGTTGAAGTAACGGTTCGTCGTGATG
GTCAGGTATATGAAATTGCTTTTGAAAATGGCGACAAAGTATCAGACCTAACGGTAACAGGTACTTGTGGCCGTCGTAAT
CGTGGTACCAGTGTTCACTTTTGGCCTGATGCGAGCTATTTTGATTCAGCAAAATTCTCTGTTACTCGTCTTATTAATAA
TCTAAAAGCTAAAGCAGTACTTTGTCCTGGTTTAGAAATTGTTTTTAAAGATAAAGTTAACGATAAAGAGCATAACTGGT
GTTATGAAGATGGTCTTAAAGATTACCTTCTTGAAGGTGTAACTGGCTTTACATTATTACCAGAAATTCCATTTACGGGT
GCTTTTTCTGGTACTGGTGAAGCGGCTGATTGGGCGGTAACTTGGCTACCTGAAGGCGGTGAGCTAGTTGCTGAAAGTTA
TGTAAACCTTATTCCTACAGCACAAGGTGGTACGCACGTAAATGGCATGCGCCAAGGTCTTCTTGATGCAATGCGTGAGT
TTTGTGAGTTCCGTAACCTTGTTCCTCGTGGTGTTAAGCTAACTGCTGATGATATTTGGGACCGTTGTGCCTACGTATTA
TCGGTGAAAATGCAAGATCCACAATTTGCTGGCCAAACTAAAGAGCGTTTATCTTCACGTCAGTGTGCAGCGTTTGTTTC
TGGTGTGGTAAAAGATGCATTCAGCTTATGGTTGAATGAGAAACCACAAATTGCAGAGCAACTTGCTGAAGCATGTATTG
CGAATGCGCACCGTCGTATGCGTGCAGCTAAAAAAGTGGTTCGTAAGAAAATAGCTTCTGGCCCAGCTCTTCCTGGTAAA
TTGACCGATTGTTCTGTTCAAGATTTGGCTCGTACTGAACTTTTCTTAGTGGAAGGGGACTCGGCGGGTGGTTCTGCAAA
ACAAGCTCGTGATCGTGAGTTCCAAGCTGTTATGCCACTGCGTGGTAAAATTCTGAATACATGGGAAGTGTCGGCTGACC
AAGTATTAGCGTCTCAAGAAGTTCATGATATTTCTGTTGCTTTAGGTATTGACCCTGATTCCGATGATTTATCAGGTTTA
CGTTACGGTAAAGTATGTATTCTTGCCGATGCCGATTCTGATGGTCTTCACATTGCAACCTTGCTATGTGCACTTTATCT
AAAACATTTCAAAGCACTGGTTCAGGCTGGTCACGTTTATGTTGCCATGCCTCCTTTGTATCGTATTGATTGCGGTAAAG
AGGTGTTCTATGCACTTGATGAAGAAGAAAAAGAAGGTGTATTAGACCGTCTAAGCAATAAGCGCGCAAAAATTAACGTA
CAGCGATTTAAAGGATTGGGTGAGATGAACCCACTTCAATTGCGTGAAACGACGATGGATCCAAATACACGACGTCTTGT
TCAGTTAACAATCGATGATGATGAACAAACTGATGAAATGATGGATATGCTTTTAGGCAAGAAACGTGCAGACGATCGTC
GCTCTTGGCTACAATCTAATGGCGATCAGGCAGAGGTATAA

Upstream 100 bases:

>100_bases
TAATACAAAGCCACATATTTAGAGTGGAATAGGGGCGTTTGCTTGACTTTCCCTTCTCCCTTTCAGACTATGTGACAAAT
TTTAAGAAGCCTTCCGAGAT

Downstream 100 bases:

>100_bases
AAATGAGTACAGAAATGTCCTTAGAAGGTGTTGAACAGCTGCCACTGCGCAAGTTTACAGAAGACGCTTATCTAAATTAT
TCCATGTACGTAATCATGGA

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 626; Mature: 625

Protein sequence:

>626_residues
MTEQYNAGAIEVLNGLEPVRRRPGMYTDTVRPNHLGQEVIDNSVDEALAGHAKKVEVILHADQSLEVIDDGRGMPVDIHP
VEKVSGVELILCKLHAGGKFSGKNYEFSGGLHGVGISVVNALSKRVEVTVRRDGQVYEIAFENGDKVSDLTVTGTCGRRN
RGTSVHFWPDASYFDSAKFSVTRLINNLKAKAVLCPGLEIVFKDKVNDKEHNWCYEDGLKDYLLEGVTGFTLLPEIPFTG
AFSGTGEAADWAVTWLPEGGELVAESYVNLIPTAQGGTHVNGMRQGLLDAMREFCEFRNLVPRGVKLTADDIWDRCAYVL
SVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFSLWLNEKPQIAEQLAEACIANAHRRMRAAKKVVRKKIASGPALPGK
LTDCSVQDLARTELFLVEGDSAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHDISVALGIDPDSDDLSGL
RYGKVCILADADSDGLHIATLLCALYLKHFKALVQAGHVYVAMPPLYRIDCGKEVFYALDEEEKEGVLDRLSNKRAKINV
QRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDDEQTDEMMDMLLGKKRADDRRSWLQSNGDQAEV

Sequences:

>Translated_626_residues
MTEQYNAGAIEVLNGLEPVRRRPGMYTDTVRPNHLGQEVIDNSVDEALAGHAKKVEVILHADQSLEVIDDGRGMPVDIHP
VEKVSGVELILCKLHAGGKFSGKNYEFSGGLHGVGISVVNALSKRVEVTVRRDGQVYEIAFENGDKVSDLTVTGTCGRRN
RGTSVHFWPDASYFDSAKFSVTRLINNLKAKAVLCPGLEIVFKDKVNDKEHNWCYEDGLKDYLLEGVTGFTLLPEIPFTG
AFSGTGEAADWAVTWLPEGGELVAESYVNLIPTAQGGTHVNGMRQGLLDAMREFCEFRNLVPRGVKLTADDIWDRCAYVL
SVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFSLWLNEKPQIAEQLAEACIANAHRRMRAAKKVVRKKIASGPALPGK
LTDCSVQDLARTELFLVEGDSAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHDISVALGIDPDSDDLSGL
RYGKVCILADADSDGLHIATLLCALYLKHFKALVQAGHVYVAMPPLYRIDCGKEVFYALDEEEKEGVLDRLSNKRAKINV
QRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDDEQTDEMMDMLLGKKRADDRRSWLQSNGDQAEV
>Mature_625_residues
TEQYNAGAIEVLNGLEPVRRRPGMYTDTVRPNHLGQEVIDNSVDEALAGHAKKVEVILHADQSLEVIDDGRGMPVDIHPV
EKVSGVELILCKLHAGGKFSGKNYEFSGGLHGVGISVVNALSKRVEVTVRRDGQVYEIAFENGDKVSDLTVTGTCGRRNR
GTSVHFWPDASYFDSAKFSVTRLINNLKAKAVLCPGLEIVFKDKVNDKEHNWCYEDGLKDYLLEGVTGFTLLPEIPFTGA
FSGTGEAADWAVTWLPEGGELVAESYVNLIPTAQGGTHVNGMRQGLLDAMREFCEFRNLVPRGVKLTADDIWDRCAYVLS
VKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFSLWLNEKPQIAEQLAEACIANAHRRMRAAKKVVRKKIASGPALPGKL
TDCSVQDLARTELFLVEGDSAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHDISVALGIDPDSDDLSGLR
YGKVCILADADSDGLHIATLLCALYLKHFKALVQAGHVYVAMPPLYRIDCGKEVFYALDEEEKEGVLDRLSNKRAKINVQ
RFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDDEQTDEMMDMLLGKKRADDRRSWLQSNGDQAEV

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=591, Percent_Identity=25.7191201353638, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI19913406, Length=592, Percent_Identity=25.5067567567568, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1789408, Length=628, Percent_Identity=80.7324840764331, Blast_Score=1074, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=556, Percent_Identity=40.4676258992806, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17535065, Length=636, Percent_Identity=24.3710691823899, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI212645845, Length=599, Percent_Identity=24.0400667779633, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=229, Percent_Identity=28.3842794759825, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=622, Percent_Identity=25.4019292604502, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17136538, Length=590, Percent_Identity=24.0677966101695, Blast_Score=123, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 69095; Mature: 68964

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQYNAGAIEVLNGLEPVRRRPGMYTDTVRPNHLGQEVIDNSVDEALAGHAKKVEVILH
CCCCCCCCHHHHHHCCHHHHHCCCCCCCCCCCCCCCHHHHHCHHHHHHHCCCEEEEEEEE
ADQSLEVIDDGRGMPVDIHPVEKVSGVELILCKLHAGGKFSGKNYEFSGGLHGVGISVVN
CCCCCEEEECCCCCCEECCCCCCCCCCEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHH
ALSKRVEVTVRRDGQVYEIAFENGDKVSDLTVTGTCGRRNRGTSVHFWPDASYFDSAKFS
HHHHHEEEEEECCCEEEEEEECCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCCCCHHH
VTRLINNLKAKAVLCPGLEIVFKDKVNDKEHNWCYEDGLKDYLLEGVTGFTLLPEIPFTG
HHHHHHHHCCCEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCC
AFSGTGEAADWAVTWLPEGGELVAESYVNLIPTAQGGTHVNGMRQGLLDAMREFCEFRNL
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
VPRGVKLTADDIWDRCAYVLSVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFSLWLNE
CCCCCEEEHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KPQIAEQLAEACIANAHRRMRAAKKVVRKKIASGPALPGKLTDCSVQDLARTELFLVEGD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEEEEECC
SAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHDISVALGIDPDSDDLSGL
CCCCCHHHHHCCCHHEECCCCCCEEEEECCCHHHHHHHCCCEEEEEEEECCCCCCCCCCC
RYGKVCILADADSDGLHIATLLCALYLKHFKALVQAGHVYVAMPPLYRIDCGKEVFYALD
EECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCCHHHEECC
EEEKEGVLDRLSNKRAKINVQRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDDEQTDEM
CHHHHHHHHHHCCCCEEEEHHHHCCCCCCCCCEEEECCCCCCCEEEEEEEECCCCHHHHH
MDMLLGKKRADDRRSWLQSNGDQAEV
HHHHHCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TEQYNAGAIEVLNGLEPVRRRPGMYTDTVRPNHLGQEVIDNSVDEALAGHAKKVEVILH
CCCCCCCHHHHHHCCHHHHHCCCCCCCCCCCCCCCHHHHHCHHHHHHHCCCEEEEEEEE
ADQSLEVIDDGRGMPVDIHPVEKVSGVELILCKLHAGGKFSGKNYEFSGGLHGVGISVVN
CCCCCEEEECCCCCCEECCCCCCCCCCEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHH
ALSKRVEVTVRRDGQVYEIAFENGDKVSDLTVTGTCGRRNRGTSVHFWPDASYFDSAKFS
HHHHHEEEEEECCCEEEEEEECCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCCCCHHH
VTRLINNLKAKAVLCPGLEIVFKDKVNDKEHNWCYEDGLKDYLLEGVTGFTLLPEIPFTG
HHHHHHHHCCCEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCC
AFSGTGEAADWAVTWLPEGGELVAESYVNLIPTAQGGTHVNGMRQGLLDAMREFCEFRNL
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
VPRGVKLTADDIWDRCAYVLSVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFSLWLNE
CCCCCEEEHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KPQIAEQLAEACIANAHRRMRAAKKVVRKKIASGPALPGKLTDCSVQDLARTELFLVEGD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEEEEECC
SAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHDISVALGIDPDSDDLSGL
CCCCCHHHHHCCCHHEECCCCCCEEEEECCCHHHHHHHCCCEEEEEEEECCCCCCCCCCC
RYGKVCILADADSDGLHIATLLCALYLKHFKALVQAGHVYVAMPPLYRIDCGKEVFYALD
EECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCCHHHEECC
EEEKEGVLDRLSNKRAKINVQRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDDEQTDEM
CHHHHHHHHHHCCCCEEEEHHHHCCCCCCCCCEEEECCCCCCCEEEEEEEECCCCHHHHH
MDMLLGKKRADDRRSWLQSNGDQAEV
HHHHHCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2170028; 8388096; 9278503; 8227000; 8980775 [H]