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The map label for this gene is degS [H]

Identifier: 59712833

GI number: 59712833

Start: 2496873

End: 2497937

Strand: Direct

Name: degS [H]

Synonym: VF_2226

Alternate gene names: 59712833

Gene position: 2496873-2497937 (Clockwise)

Preceding gene: 59712832

Following gene: 59712840

Centisome position: 86.17

GC content: 40.66

Gene sequence:

>1065_bases
ATGCTCTCATTCTTATTGCGTTCAGCTATGATAGGTATCGCAACAGCTGCGATATTGCTGTTTGCATTCCCTAACCTACG
TTCAAACATCACGCTAGATCAAGGTGCGATAGAGCGAACTTCCCAGAACAGTAAGCAAATTTCATTTAATTATGCCGTTC
GTCGAGCCGCTCCTGCTGTTGTCAATATCTATAGCCGTAAATACAACGATAATGACCGTCTACGTTTAGATACAGAAGGC
TTAGGTTCTGGTGTTATCGTGAGTGATAAAGGCTACATCATTACTAACTATCATGTTGTTGCCGATGCTGATCAAGTCAT
TGTAGCTCTTCAAGATGGGCGCTTATTTAACTCTCAATTAATTGGTAAAGATAAACGTACTGACTTAGCTGTATTACAAA
TTAAAGATGCTAACCTCCCTGTTATTCCATTAAACCCAAACTATCAAGCTGATATTGGCGATGTTGTGCTGGCTATTGGT
AACCCATATAACCTAGGACAAACCACAACCTTCGGGATTATTTCTGCTACCGGACGAGCGGCGTTGAGTGCCGACGGTAC
ACAAGGTTTAATTCAAACAGACGCAGCGATCAATCGAGGTAACTCAGGTGGCGCTCTAGTCAATACCAGAGGTGAACTCG
TTGGTATCAATACCGCCTCATTCCAACAAGCCACTGAGCTAGAAACTTATGGTATTTCATTTGCTATTCCTTATGCTCTT
GCGGATAAAATCATGCGTCGATTGATTGCAGATGGACAAGTGATTCGTGGTTATATTGGTATTGATGGAAGAGACATCAA
TCCAGCCATGGCAAGATTAATGAATGCAGATAATATAAACGGTATTTTAGTTTTAGGTGTTTCCCCAAATAGCCCAGCAG
AAAAAGCCGGTATCATTACTCAAGATATAATCACCAGTATCGATGGTAAAAGTGTTACTAATAGACAAAGCGTCATTGAT
ATCGTAACAGAGTTAAGACCCGGTACTGAAGTGGATGTTGATGTAATTCGCAAAGGGAAAACTGAGACGATTAAAGTAAC
TATTGAAGAAGACCCAAACAATTAA

Upstream 100 bases:

>100_bases
CTCATTCTTTGAGTGCTTGCTTCTCCATCCTCTACTTTTTAACTAGATTCAATGTTATCCTAATCAATTATTCTTTTTAT
TCATCAAGGTTAGGAAGCCA

Downstream 100 bases:

>100_bases
TAAAAAAATAGCGCATTAAAAAATGCGCTATTTTGTATCTAAGCAATCGCAATGACTAAAGGTTATTCACTTTCTTCTTC
AGTCGTTGTGCTCTCACCAT

Product: serine endoprotease DegS, periplasmic

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MLSFLLRSAMIGIATAAILLFAFPNLRSNITLDQGAIERTSQNSKQISFNYAVRRAAPAVVNIYSRKYNDNDRLRLDTEG
LGSGVIVSDKGYIITNYHVVADADQVIVALQDGRLFNSQLIGKDKRTDLAVLQIKDANLPVIPLNPNYQADIGDVVLAIG
NPYNLGQTTTFGIISATGRAALSADGTQGLIQTDAAINRGNSGGALVNTRGELVGINTASFQQATELETYGISFAIPYAL
ADKIMRRLIADGQVIRGYIGIDGRDINPAMARLMNADNINGILVLGVSPNSPAEKAGIITQDIITSIDGKSVTNRQSVID
IVTELRPGTEVDVDVIRKGKTETIKVTIEEDPNN

Sequences:

>Translated_354_residues
MLSFLLRSAMIGIATAAILLFAFPNLRSNITLDQGAIERTSQNSKQISFNYAVRRAAPAVVNIYSRKYNDNDRLRLDTEG
LGSGVIVSDKGYIITNYHVVADADQVIVALQDGRLFNSQLIGKDKRTDLAVLQIKDANLPVIPLNPNYQADIGDVVLAIG
NPYNLGQTTTFGIISATGRAALSADGTQGLIQTDAAINRGNSGGALVNTRGELVGINTASFQQATELETYGISFAIPYAL
ADKIMRRLIADGQVIRGYIGIDGRDINPAMARLMNADNINGILVLGVSPNSPAEKAGIITQDIITSIDGKSVTNRQSVID
IVTELRPGTEVDVDVIRKGKTETIKVTIEEDPNN
>Mature_354_residues
MLSFLLRSAMIGIATAAILLFAFPNLRSNITLDQGAIERTSQNSKQISFNYAVRRAAPAVVNIYSRKYNDNDRLRLDTEG
LGSGVIVSDKGYIITNYHVVADADQVIVALQDGRLFNSQLIGKDKRTDLAVLQIKDANLPVIPLNPNYQADIGDVVLAIG
NPYNLGQTTTFGIISATGRAALSADGTQGLIQTDAAINRGNSGGALVNTRGELVGINTASFQQATELETYGISFAIPYAL
ADKIMRRLIADGQVIRGYIGIDGRDINPAMARLMNADNINGILVLGVSPNSPAEKAGIITQDIITSIDGKSVTNRQSVID
IVTELRPGTEVDVDVIRKGKTETIKVTIEEDPNN

Specific function: Senses the OMP signal triggered by the accumulation of unassembled porins in the envelope and then initiates rseA degradation by cleaving it in its periplasmic domain, making it an attractive substrate for subsequent cleavage by rseP [H]

COG id: COG0265

COG function: function code O; Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Homo sapiens, GI4506141, Length=346, Percent_Identity=33.5260115606936, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI22129776, Length=311, Percent_Identity=36.3344051446945, Blast_Score=141, Evalue=7e-34,
Organism=Homo sapiens, GI24308541, Length=288, Percent_Identity=32.2916666666667, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI7019477, Length=288, Percent_Identity=34.7222222222222, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1789630, Length=354, Percent_Identity=54.8022598870056, Blast_Score=345, Evalue=3e-96,
Organism=Escherichia coli, GI1786356, Length=276, Percent_Identity=43.4782608695652, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1789629, Length=290, Percent_Identity=41.3793103448276, Blast_Score=213, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24646839, Length=292, Percent_Identity=33.2191780821918, Blast_Score=125, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR009003
- InterPro:   IPR011783
- InterPro:   IPR001254
- InterPro:   IPR001940 [H]

Pfam domain/function: PF00595 PDZ; PF00089 Trypsin [H]

EC number: 3.4.21.- [C]

Molecular weight: Translated: 37971; Mature: 37971

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSFLLRSAMIGIATAAILLFAFPNLRSNITLDQGAIERTSQNSKQISFNYAVRRAAPAV
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHCCCCCCEEEEEEHHHHCCCCE
VNIYSRKYNDNDRLRLDTEGLGSGVIVSDKGYIITNYHVVADADQVIVALQDGRLFNSQL
EEEEHHCCCCCCCEEEECCCCCCCEEEECCCEEEEEEEEEECCCEEEEEEECCCEECHHH
IGKDKRTDLAVLQIKDANLPVIPLNPNYQADIGDVVLAIGNPYNLGQTTTFGIISATGRA
CCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCEEEEEECCCCCCCCCEEEEEEEECCCE
ALSADGTQGLIQTDAAINRGNSGGALVNTRGELVGINTASFQQATELETYGISFAIPYAL
EECCCCCCCEEEECHHHCCCCCCCEEEECCCCEEECCCHHCHHHHHHHHCCEEEEEHHHH
ADKIMRRLIADGQVIRGYIGIDGRDINPAMARLMNADNINGILVLGVSPNSPAEKAGIIT
HHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCHHHCCCCH
QDIITSIDGKSVTNRQSVIDIVTELRPGTEVDVDVIRKGKTETIKVTIEEDPNN
HHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEEEECCCCC
>Mature Secondary Structure
MLSFLLRSAMIGIATAAILLFAFPNLRSNITLDQGAIERTSQNSKQISFNYAVRRAAPAV
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHCCCCCCEEEEEEHHHHCCCCE
VNIYSRKYNDNDRLRLDTEGLGSGVIVSDKGYIITNYHVVADADQVIVALQDGRLFNSQL
EEEEHHCCCCCCCEEEECCCCCCCEEEECCCEEEEEEEEEECCCEEEEEEECCCEECHHH
IGKDKRTDLAVLQIKDANLPVIPLNPNYQADIGDVVLAIGNPYNLGQTTTFGIISATGRA
CCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCEEEEEECCCCCCCCCEEEEEEEECCCE
ALSADGTQGLIQTDAAINRGNSGGALVNTRGELVGINTASFQQATELETYGISFAIPYAL
EECCCCCCCEEEECHHHCCCCCCCEEEECCCCEEECCCHHCHHHHHHHHCCEEEEEHHHH
ADKIMRRLIADGQVIRGYIGIDGRDINPAMARLMNADNINGILVLGVSPNSPAEKAGIIT
HHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCHHHCCCCH
QDIITSIDGKSVTNRQSVIDIVTELRPGTEVDVDVIRKGKTETIKVTIEEDPNN
HHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]