Click here to switch to the map view.

The map label for this gene is mtn

Identifier: 59712735

GI number: 59712735

Start: 2374365

End: 2375060

Strand: Direct

Name: mtn

Synonym: VF_2128

Alternate gene names: 59712735

Gene position: 2374365-2375060 (Clockwise)

Preceding gene: 59712733

Following gene: 59712736

Centisome position: 81.94

GC content: 40.37

Gene sequence:

>696_bases
ATGAAAATTGGCATTATTGGCGCTATGGAACAAGAAGTTGCCATCCTTAAAGACAAAATTGAAGGTCTATCAACAGTAAC
TAAAGCTGGTTGTACTTTTTATACTGGTACATTAAATGGCGCTGACGTTGTTTTATTACAGTCAGGGATTGGTAAAGTTG
CAGCAGCAGTTGGAACAACTCTTCTTATTGCTGAGCATAATGTTGATGTCGTTCTTAATACAGGTTCTGCGGGTGGCTTT
GATTCATCATTAAATCTTGGTGACGTGGTCATCTCAACAGAAGTTCGCCACCACGATGCTGACGTAACTGCTTTTGGTTA
CGAAATGGGACAAATGGCTCAACAGCCAGCTGCATTTATTGCTGACGAAAAGCTAATCACAACTGCTGAGCAAGCATTAA
CTGAAATGAGTGATAAGCACGCTGTTCGCGGATTAATTTGTACTGGTGATGTTTTTGTTTGTACTCCTGAGCGCCAAGAG
TTTATCCGTACTCACTTCCCTTCAGTAATTGCTGTAGAAATGGAAGCCTCAGCGATTGCTCAAACATGTCACCAATTCAA
TACACCTTTTGTTGTCGTTCGAGCTATTTCAGACGTAGCAGATAAAGAGTCACCAATGAGCTTTGATGAATTCCTTCCTC
TTGCAGCACAAAGCTCATCAGAAATGGTATTAAACATGGTTACTTTATTAAAGTAA

Upstream 100 bases:

>100_bases
ACTTATTTATTTCAGAATAAACATCAAATTCCATATCTCCTAAGTACTATATACCTATGTTACAATCATCCACTACTCTA
ACTTTTATTGAGATATAAAC

Downstream 100 bases:

>100_bases
TCTTATTTAATTGAGTCAGTTTCTCTGACTCAATTTTATCTTCTTTTATATATAAAGAATCACCTCATGCTAGATAAATT
AACTCTCTTTATTTCTGATT

Product: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

Products: NA

Alternate protein names: MTA/SAH nucleosidase; MTAN; 5'-methylthioadenosine nucleosidase; MTA nucleosidase; S-adenosylhomocysteine nucleosidase; AdoHcy nucleosidase; SAH nucleosidase; SRH nucleosidase

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MKIGIIGAMEQEVAILKDKIEGLSTVTKAGCTFYTGTLNGADVVLLQSGIGKVAAAVGTTLLIAEHNVDVVLNTGSAGGF
DSSLNLGDVVISTEVRHHDADVTAFGYEMGQMAQQPAAFIADEKLITTAEQALTEMSDKHAVRGLICTGDVFVCTPERQE
FIRTHFPSVIAVEMEASAIAQTCHQFNTPFVVVRAISDVADKESPMSFDEFLPLAAQSSSEMVLNMVTLLK

Sequences:

>Translated_231_residues
MKIGIIGAMEQEVAILKDKIEGLSTVTKAGCTFYTGTLNGADVVLLQSGIGKVAAAVGTTLLIAEHNVDVVLNTGSAGGF
DSSLNLGDVVISTEVRHHDADVTAFGYEMGQMAQQPAAFIADEKLITTAEQALTEMSDKHAVRGLICTGDVFVCTPERQE
FIRTHFPSVIAVEMEASAIAQTCHQFNTPFVVVRAISDVADKESPMSFDEFLPLAAQSSSEMVLNMVTLLK
>Mature_231_residues
MKIGIIGAMEQEVAILKDKIEGLSTVTKAGCTFYTGTLNGADVVLLQSGIGKVAAAVGTTLLIAEHNVDVVLNTGSAGGF
DSSLNLGDVVISTEVRHHDADVTAFGYEMGQMAQQPAAFIADEKLITTAEQALTEMSDKHAVRGLICTGDVFVCTPERQE
FIRTHFPSVIAVEMEASAIAQTCHQFNTPFVVVRAISDVADKESPMSFDEFLPLAAQSSSEMVLNMVTLLK

Specific function: Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively

COG id: COG0775

COG function: function code F; Nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/UDP phosphorylase family. MtnN subfamily

Homologues:

Organism=Escherichia coli, GI1786354, Length=230, Percent_Identity=58.695652173913, Blast_Score=241, Evalue=3e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTNN_VIBF1 (Q5E2X3)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205511.1
- HSSP:   P0AF12
- ProteinModelPortal:   Q5E2X3
- SMR:   Q5E2X3
- STRING:   Q5E2X3
- GeneID:   3279308
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2128
- NMPDR:   fig|312309.3.peg.2128
- eggNOG:   COG0775
- HOGENOM:   HBG367723
- OMA:   AMEQEVT
- PhylomeDB:   Q5E2X3
- ProtClustDB:   PRK05584
- BioCyc:   VFIS312309:VF2128-MONOMER
- HAMAP:   MF_01684
- InterPro:   IPR010049
- InterPro:   IPR018017
- InterPro:   IPR000845
- PANTHER:   PTHR21234
- TIGRFAMs:   TIGR01704

Pfam domain/function: PF01048 PNP_UDP_1

EC number: =3.2.2.9

Molecular weight: Translated: 24583; Mature: 24583

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: ACT_SITE 12-12 BINDING 78-78 BINDING 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIIGAMEQEVAILKDKIEGLSTVTKAGCTFYTGTLNGADVVLLQSGIGKVAAAVGTT
CEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCHHHHHHHCCE
LLIAEHNVDVVLNTGSAGGFDSSLNLGDVVISTEVRHHDADVTAFGYEMGQMAQQPAAFI
EEEEECCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEHHHHHHHHHCCHHHH
ADEKLITTAEQALTEMSDKHAVRGLICTGDVFVCTPERQEFIRTHFPSVIAVEMEASAIA
CCCHHHHHHHHHHHHHHHHHHHEEEEEECCEEEECCCHHHHHHHCCCCEEEEEEHHHHHH
QTCHQFNTPFVVVRAISDVADKESPMSFDEFLPLAAQSSSEMVLNMVTLLK
HHHHHCCCCEEEEEEHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKIGIIGAMEQEVAILKDKIEGLSTVTKAGCTFYTGTLNGADVVLLQSGIGKVAAAVGTT
CEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCHHHHHHHCCE
LLIAEHNVDVVLNTGSAGGFDSSLNLGDVVISTEVRHHDADVTAFGYEMGQMAQQPAAFI
EEEEECCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEHHHHHHHHHCCHHHH
ADEKLITTAEQALTEMSDKHAVRGLICTGDVFVCTPERQEFIRTHFPSVIAVEMEASAIA
CCCHHHHHHHHHHHHHHHHHHHEEEEEECCEEEECCCHHHHHHHCCCCEEEEEEHHHHHH
QTCHQFNTPFVVVRAISDVADKESPMSFDEFLPLAAQSSSEMVLNMVTLLK
HHHHHCCCCEEEEEEHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA