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The map label for this gene is gltD [H]

Identifier: 59712732

GI number: 59712732

Start: 2366162

End: 2367634

Strand: Direct

Name: gltD [H]

Synonym: VF_2125

Alternate gene names: 59712732

Gene position: 2366162-2367634 (Clockwise)

Preceding gene: 59712731

Following gene: 59712733

Centisome position: 81.66

GC content: 42.43

Gene sequence:

>1473_bases
ATGGGTAAGCCTACTGGTTTTTTAGAGCATGGTCGTGAACTTCCAAAGAAGATCGACCCTAGTGTTCGAATTCAAGACAA
TAAAGAATTCGTCTTAAACGAAGAGTTTGGCGACAAAATTAATACTCAAGCATCTCGCTGTATGGATTGTGGTGTACCGT
TTTGTCACAACGGATGCCCTATCGGTAACATTATTCCTGAATTTAACGATGCCGTTTATCGTGATAGTTGGGAAGAAGCA
TGGAAGATTTTAAGTTCAACAAATAACTTTCCAGAATTTACTGGTCGAGTATGTCCAGCTCCTTGTGAAAGTGCCTGTGT
TCTTGGTATCAACCAAGATCCAATCACAATTTGTAATATCGAGAAAACAATTGTAGAGACGGCATATCGTGAGGGCTACG
CACAACCGAAAAAACCTCGTTCACGAACAGGTAAAACGGTAGCCATTGTTGGTTCAGGTCCTGCTGGCTTAGCAGCAGCA
GAGCAACTTAATAGTGCTGGCCATTCAGTCACCGTATATGAGCGTGACGAAAAAGTAGGTGGACTACTTCGATTTGGCAT
TCCAGATTTTAAATTAGGAATGGACATTATTGATCGTAAAATCAATTTAATGGCTGATGCTGGTATTAAGTTCGAAGTTA
ACGCTCATATTGGTATTGATGTTAATGCACAGCAACTACGCCAAGATTTTGATGTAGTTCTACTTACTGGTGGTTCTACC
GTGCCACGAGACCTGCCTATTCCTGGTCGTGACCTCAATGGGGTTCACTTTGCAATGGAGTTCCTAGGTCAAAATAACCG
CCGTGCTAATAACATGGATTTAAAAACCAAAGAAATCCACGCTAAAGGCAAACACGTTGTAGTTATTGGCGGTGGCGATA
CAGGTTCTGACTGTGTTGGTACTTCTAATCGCCATGGTGCAGCAAGCATTACTCAGGTAGAGATCATGCCGATCCCACCA
GAAAAACGCCCTGCAAATATGCCTTGGCCTCAATATCCGATGATCATGAAAACAACCACTTCTCACGAAGAAGGTTGTGA
ACGTCATTGGAATATTTTAACCAAAGAATTTATCTCTGATGATGCAGGTAATGTAAAAGGCCTTCGTATTGCAGATATCG
TTTGGCAAGATGCGAAGCCAGGTGAACGCCCAGGCTTTGATGAAGTTGCAGGCAGTGAACGAGTTATTCCTTGTGACCAA
GCCTTCTTAGCTATGGGCTTCTTACATCCAGAACCAACGGGTGTATTAGCACAATTAGACATCAAGTTAGACGATCGTGG
TAATGTGGCTACAGATGGCTTTGCGACAAATCAAAAAGGCGTTTTTGCGGCAGGAGATATGCGAACAGGACAATCCCTGG
TTGTTCGTTGTATTAATGAAGGTCGTGAATGTGCTCGTGAAATAGATAGCTATCTAATGGGTGGCTCTAATTTAGAAGCA
AAAGCGGATTCATTAATGCTTTCTGCTTCATAA

Upstream 100 bases:

>100_bases
CCTCTTTGGCTAAAGTAATGCCGCGAGATTACAAAGCGGTACTTCAAAAGCGTAAAGCTGAAGCACAACAAGCACAAACG
GAAGAAGTGGAGGCAGTATA

Downstream 100 bases:

>100_bases
TTTACTAAATTCAGGTTTCCTTCCAAACCTTTGGCCAACATTTCGATGTTGGCCTTTTTTCTGCCTCATAGCTAAAAAGC
GACAATTTATAGCATTTTGG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MGKPTGFLEHGRELPKKIDPSVRIQDNKEFVLNEEFGDKINTQASRCMDCGVPFCHNGCPIGNIIPEFNDAVYRDSWEEA
WKILSSTNNFPEFTGRVCPAPCESACVLGINQDPITICNIEKTIVETAYREGYAQPKKPRSRTGKTVAIVGSGPAGLAAA
EQLNSAGHSVTVYERDEKVGGLLRFGIPDFKLGMDIIDRKINLMADAGIKFEVNAHIGIDVNAQQLRQDFDVVLLTGGST
VPRDLPIPGRDLNGVHFAMEFLGQNNRRANNMDLKTKEIHAKGKHVVVIGGGDTGSDCVGTSNRHGAASITQVEIMPIPP
EKRPANMPWPQYPMIMKTTTSHEEGCERHWNILTKEFISDDAGNVKGLRIADIVWQDAKPGERPGFDEVAGSERVIPCDQ
AFLAMGFLHPEPTGVLAQLDIKLDDRGNVATDGFATNQKGVFAAGDMRTGQSLVVRCINEGRECAREIDSYLMGGSNLEA
KADSLMLSAS

Sequences:

>Translated_490_residues
MGKPTGFLEHGRELPKKIDPSVRIQDNKEFVLNEEFGDKINTQASRCMDCGVPFCHNGCPIGNIIPEFNDAVYRDSWEEA
WKILSSTNNFPEFTGRVCPAPCESACVLGINQDPITICNIEKTIVETAYREGYAQPKKPRSRTGKTVAIVGSGPAGLAAA
EQLNSAGHSVTVYERDEKVGGLLRFGIPDFKLGMDIIDRKINLMADAGIKFEVNAHIGIDVNAQQLRQDFDVVLLTGGST
VPRDLPIPGRDLNGVHFAMEFLGQNNRRANNMDLKTKEIHAKGKHVVVIGGGDTGSDCVGTSNRHGAASITQVEIMPIPP
EKRPANMPWPQYPMIMKTTTSHEEGCERHWNILTKEFISDDAGNVKGLRIADIVWQDAKPGERPGFDEVAGSERVIPCDQ
AFLAMGFLHPEPTGVLAQLDIKLDDRGNVATDGFATNQKGVFAAGDMRTGQSLVVRCINEGRECAREIDSYLMGGSNLEA
KADSLMLSAS
>Mature_489_residues
GKPTGFLEHGRELPKKIDPSVRIQDNKEFVLNEEFGDKINTQASRCMDCGVPFCHNGCPIGNIIPEFNDAVYRDSWEEAW
KILSSTNNFPEFTGRVCPAPCESACVLGINQDPITICNIEKTIVETAYREGYAQPKKPRSRTGKTVAIVGSGPAGLAAAE
QLNSAGHSVTVYERDEKVGGLLRFGIPDFKLGMDIIDRKINLMADAGIKFEVNAHIGIDVNAQQLRQDFDVVLLTGGSTV
PRDLPIPGRDLNGVHFAMEFLGQNNRRANNMDLKTKEIHAKGKHVVVIGGGDTGSDCVGTSNRHGAASITQVEIMPIPPE
KRPANMPWPQYPMIMKTTTSHEEGCERHWNILTKEFISDDAGNVKGLRIADIVWQDAKPGERPGFDEVAGSERVIPCDQA
FLAMGFLHPEPTGVLAQLDIKLDDRGNVATDGFATNQKGVFAAGDMRTGQSLVVRCINEGRECAREIDSYLMGGSNLEAK
ADSLMLSAS

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=496, Percent_Identity=23.991935483871, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI111118983, Length=153, Percent_Identity=32.0261437908497, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI111118981, Length=153, Percent_Identity=32.0261437908497, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1789606, Length=455, Percent_Identity=34.2857142857143, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI1788811, Length=448, Percent_Identity=34.375, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI87082180, Length=442, Percent_Identity=33.0316742081448, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1788468, Length=438, Percent_Identity=26.7123287671233, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1789243, Length=428, Percent_Identity=28.7383177570093, Blast_Score=112, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17570289, Length=493, Percent_Identity=45.0304259634888, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71984108, Length=473, Percent_Identity=23.8900634249471, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17543860, Length=169, Percent_Identity=35.5029585798817, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320030, Length=485, Percent_Identity=44.5360824742268, Blast_Score=431, Evalue=1e-121,
Organism=Drosophila melanogaster, GI28574881, Length=485, Percent_Identity=50.1030927835052, Blast_Score=482, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24665539, Length=485, Percent_Identity=50.1030927835052, Blast_Score=482, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24665547, Length=485, Percent_Identity=50.1030927835052, Blast_Score=480, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24665543, Length=485, Percent_Identity=50.1030927835052, Blast_Score=480, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24640763, Length=288, Percent_Identity=28.4722222222222, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI18858217, Length=288, Percent_Identity=28.4722222222222, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53420; Mature: 53289

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKPTGFLEHGRELPKKIDPSVRIQDNKEFVLNEEFGDKINTQASRCMDCGVPFCHNGCP
CCCCCCHHHHHHHHHHHCCCCEEEECCCEEEEECHHCCHHHHHHHHHHHCCCCCCCCCCC
IGNIIPEFNDAVYRDSWEEAWKILSSTNNFPEFTGRVCPAPCESACVLGINQDPITICNI
CCCCCCCCCCHHHHCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEEECCCCCEEEECC
EKTIVETAYREGYAQPKKPRSRTGKTVAIVGSGPAGLAAAEQLNSAGHSVTVYERDEKVG
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCC
GLLRFGIPDFKLGMDIIDRKINLMADAGIKFEVNAHIGIDVNAQQLRQDFDVVLLTGGST
CEEEECCCCHHHCHHHHHHHHEEEECCCEEEEEEEEEEECCCHHHHHCCCCEEEEECCCC
VPRDLPIPGRDLNGVHFAMEFLGQNNRRANNMDLKTKEIHAKGKHVVVIGGGDTGSDCVG
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCC
TSNRHGAASITQVEIMPIPPEKRPANMPWPQYPMIMKTTTSHEEGCERHWNILTKEFISD
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCC
DAGNVKGLRIADIVWQDAKPGERPGFDEVAGSERVIPCDQAFLAMGFLHPEPTGVLAQLD
CCCCCCCEEEEEEEECCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEE
IKLDDRGNVATDGFATNQKGVFAAGDMRTGQSLVVRCINEGRECAREIDSYLMGGSNLEA
EEECCCCCEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KADSLMLSAS
CCCCEEEECC
>Mature Secondary Structure 
GKPTGFLEHGRELPKKIDPSVRIQDNKEFVLNEEFGDKINTQASRCMDCGVPFCHNGCP
CCCCCHHHHHHHHHHHCCCCEEEECCCEEEEECHHCCHHHHHHHHHHHCCCCCCCCCCC
IGNIIPEFNDAVYRDSWEEAWKILSSTNNFPEFTGRVCPAPCESACVLGINQDPITICNI
CCCCCCCCCCHHHHCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEEECCCCCEEEECC
EKTIVETAYREGYAQPKKPRSRTGKTVAIVGSGPAGLAAAEQLNSAGHSVTVYERDEKVG
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCC
GLLRFGIPDFKLGMDIIDRKINLMADAGIKFEVNAHIGIDVNAQQLRQDFDVVLLTGGST
CEEEECCCCHHHCHHHHHHHHEEEECCCEEEEEEEEEEECCCHHHHHCCCCEEEEECCCC
VPRDLPIPGRDLNGVHFAMEFLGQNNRRANNMDLKTKEIHAKGKHVVVIGGGDTGSDCVG
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCC
TSNRHGAASITQVEIMPIPPEKRPANMPWPQYPMIMKTTTSHEEGCERHWNILTKEFISD
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCC
DAGNVKGLRIADIVWQDAKPGERPGFDEVAGSERVIPCDQAFLAMGFLHPEPTGVLAQLD
CCCCCCCEEEEEEEECCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEE
IKLDDRGNVATDGFATNQKGVFAAGDMRTGQSLVVRCINEGRECAREIDSYLMGGSNLEA
EEECCCCCEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KADSLMLSAS
CCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]