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The map label for this gene is mscS [H]

Identifier: 59712715

GI number: 59712715

Start: 2342969

End: 2343829

Strand: Reverse

Name: mscS [H]

Synonym: VF_2108

Alternate gene names: 59712715

Gene position: 2343829-2342969 (Counterclockwise)

Preceding gene: 59712716

Following gene: 59712714

Centisome position: 80.89

GC content: 38.79

Gene sequence:

>861_bases
ATGGCAGATCAAGGCGCTGTAACTGATTTAGCTACAACTGGCGTAGCTAACTTAGAGCATTGGCTAACAGATAATACTGA
ATTGTTTATTACTTATGGTGTAAACATTATTTCAGCTCTCGCGATTCTTTTTATCGGTAACATTATCGTAAAAAAAGTAG
CGAACAGTGTAAGTAACGTACTTAAAAAGAAAAAGATGGATAAAGCGGTTGTTGACTTTGTTCACACTCTTGTTCGTTAC
CTTTTATTCATCATCGTATTAATTGCTGCATTAGGTAAAGTTGGCGTACAAACAGCATCGGTTGTTGCTGTGATTGGTGC
GGCTGGTTTAGCGGTTGGTTTAGCGCTTCAAGGCTCTCTATCAAACTTTGCAGCTGGTGTATTGATTGTTGCTTTCCGTC
CATTTAAATCTGGCGATTATGTTGAGATTGGTGGTGTTGCGGGGTCAGTTGATTCAATTCAAATTTTCCAAACGATCCTA
ACTACACCTGATAACAAAATGGTTGTAGTACCAAATGGTGGTGTTATTGGTAGCCCAATTACAAACTATTCTCGCCATGC
AACGCGTCGTGTAGATCATGTTATTGGTGTTTCTTATAGTGCGGATCTTAAGAAAACAAAAGAAGTCATTACAAAAGTAC
TTGAGTCAGATCCTCGTGTATTGAAAACACCTGCTCCAACGGTTGGTGTTGTTGCTCTAGCTGATTCATCTGTAAACTTT
GTTGTTCGCCCGTGGTGTAAAACTGCTGAGTATTGGGATGTTTACTTTGATACGCTTCAAGCGATTAAAGAAGGCCTAGA
TAATGAAGGTATTGAGATCCCATTCCCACAAATGGATGTTCATTTAAATAAAGTAGATTAA

Upstream 100 bases:

>100_bases
TTACCCCCAAGTTAACACAGAAAGTGTTAAAATCGGCGTAATGTCAAAATAATGGCAACCCTCTCAATTTTTTGAGAATA
ACTATAAAAGGACAAATCAA

Downstream 100 bases:

>100_bases
TTTTTATTTAAATCAAAATATTAAATGCTACCTTCGGGTAGCATTTTTTTTGCCTTAACATGCATAATGATAAGGAATTA
TTTGCTGTTATTAGAGTCAT

Product: mechanosensitive channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MADQGAVTDLATTGVANLEHWLTDNTELFITYGVNIISALAILFIGNIIVKKVANSVSNVLKKKKMDKAVVDFVHTLVRY
LLFIIVLIAALGKVGVQTASVVAVIGAAGLAVGLALQGSLSNFAAGVLIVAFRPFKSGDYVEIGGVAGSVDSIQIFQTIL
TTPDNKMVVVPNGGVIGSPITNYSRHATRRVDHVIGVSYSADLKKTKEVITKVLESDPRVLKTPAPTVGVVALADSSVNF
VVRPWCKTAEYWDVYFDTLQAIKEGLDNEGIEIPFPQMDVHLNKVD

Sequences:

>Translated_286_residues
MADQGAVTDLATTGVANLEHWLTDNTELFITYGVNIISALAILFIGNIIVKKVANSVSNVLKKKKMDKAVVDFVHTLVRY
LLFIIVLIAALGKVGVQTASVVAVIGAAGLAVGLALQGSLSNFAAGVLIVAFRPFKSGDYVEIGGVAGSVDSIQIFQTIL
TTPDNKMVVVPNGGVIGSPITNYSRHATRRVDHVIGVSYSADLKKTKEVITKVLESDPRVLKTPAPTVGVVALADSSVNF
VVRPWCKTAEYWDVYFDTLQAIKEGLDNEGIEIPFPQMDVHLNKVD
>Mature_285_residues
ADQGAVTDLATTGVANLEHWLTDNTELFITYGVNIISALAILFIGNIIVKKVANSVSNVLKKKKMDKAVVDFVHTLVRYL
LFIIVLIAALGKVGVQTASVVAVIGAAGLAVGLALQGSLSNFAAGVLIVAFRPFKSGDYVEIGGVAGSVDSIQIFQTILT
TPDNKMVVVPNGGVIGSPITNYSRHATRRVDHVIGVSYSADLKKTKEVITKVLESDPRVLKTPAPTVGVVALADSSVNFV
VRPWCKTAEYWDVYFDTLQAIKEGLDNEGIEIPFPQMDVHLNKVD

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=266, Percent_Identity=50.7518796992481, Blast_Score=288, Evalue=2e-79,
Organism=Escherichia coli, GI1786670, Length=226, Percent_Identity=29.2035398230088, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI2367355, Length=259, Percent_Identity=24.3243243243243, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787591, Length=294, Percent_Identity=26.530612244898, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 30606; Mature: 30475

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADQGAVTDLATTGVANLEHWLTDNTELFITYGVNIISALAILFIGNIIVKKVANSVSNV
CCCCCCHHHHHHHCHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKKKKMDKAVVDFVHTLVRYLLFIIVLIAALGKVGVQTASVVAVIGAAGLAVGLALQGSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCH
SNFAAGVLIVAFRPFKSGDYVEIGGVAGSVDSIQIFQTILTTPDNKMVVVPNGGVIGSPI
HHHHHHEEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEECCCEECCCC
TNYSRHATRRVDHVIGVSYSADLKKTKEVITKVLESDPRVLKTPAPTVGVVALADSSVNF
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCE
VVRPWCKTAEYWDVYFDTLQAIKEGLDNEGIEIPFPQMDVHLNKVD
EEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCC
>Mature Secondary Structure 
ADQGAVTDLATTGVANLEHWLTDNTELFITYGVNIISALAILFIGNIIVKKVANSVSNV
CCCCCHHHHHHHCHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKKKKMDKAVVDFVHTLVRYLLFIIVLIAALGKVGVQTASVVAVIGAAGLAVGLALQGSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCH
SNFAAGVLIVAFRPFKSGDYVEIGGVAGSVDSIQIFQTILTTPDNKMVVVPNGGVIGSPI
HHHHHHEEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEECCCEECCCC
TNYSRHATRRVDHVIGVSYSADLKKTKEVITKVLESDPRVLKTPAPTVGVVALADSSVNF
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCE
VVRPWCKTAEYWDVYFDTLQAIKEGLDNEGIEIPFPQMDVHLNKVD
EEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]