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The map label for this gene is ygfA [H]

Identifier: 59712711

GI number: 59712711

Start: 2339283

End: 2339864

Strand: Direct

Name: ygfA [H]

Synonym: VF_2104

Alternate gene names: 59712711

Gene position: 2339283-2339864 (Clockwise)

Preceding gene: 59712710

Following gene: 59712712

Centisome position: 80.73

GC content: 41.58

Gene sequence:

>582_bases
ATGACATCTCGTCGACTCATTCGACAATCTACACGTCAACGTCGTAACGCTTTATCTCCTATTGAACAATCTCAAGCAGC
CAACGCTCTTGTCCAGCAGTGTTCTATCAACCCTGTAATTCAAAAAGCACACTCTATTGCAGTCTATTTATCTTCCGATG
GTGAAATTGATACCAATCCACTCATCACTTGGTTATGGGCACAGGGCAAACAAGTCGCTTTACCTGTAATACACCCATTC
TCAAAGGGTCATTTATTATTCCTTGAGTACACTGAAAACTCACCGCTTGTTCATAATAAATATCAGATCCTAGAACCCAA
ACTAAACAAAACTACAATTATACCGACCTCTGAATTAGATCTAATTTTGACGCCGTTAGTTGCTTTTGATCATACCGGTA
ATCGTTTAGGTATGGGCGGAGGATATTATGATCGAACCCTCTCTAATTGGCACTCCGCTCAGCAAGGTCCAATGCCGATA
GGGTTATCACACACTTGCCAGCAAGTTTCATCGTTACCGATTGAATCTTGGGATGTACCACTTCCTCAAATCATCACACC
TCAAAAAACATGGCACTGGTAA

Upstream 100 bases:

>100_bases
CATGCTCGGCTTGTACTGAGAAGCCTACGGTAAACTTTGCTGATCCGCCTTGAACCTAGGGTTCAAGGGCTACGACCACT
ACGGCACTTTGGGGTATTCC

Downstream 100 bases:

>100_bases
ATAGTTAGCCGAAACCGTTTGCTTTGCTTGTATTTTATTCATTTTTTACGCTTTACTGACTTATTTTTTCTGACGAAGAA
AAAACTCTACGCTATAATCA

Product: ligase

Products: ADP; phosphate; 5,10-methenyltetrahydrofolate

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MTSRRLIRQSTRQRRNALSPIEQSQAANALVQQCSINPVIQKAHSIAVYLSSDGEIDTNPLITWLWAQGKQVALPVIHPF
SKGHLLFLEYTENSPLVHNKYQILEPKLNKTTIIPTSELDLILTPLVAFDHTGNRLGMGGGYYDRTLSNWHSAQQGPMPI
GLSHTCQQVSSLPIESWDVPLPQIITPQKTWHW

Sequences:

>Translated_193_residues
MTSRRLIRQSTRQRRNALSPIEQSQAANALVQQCSINPVIQKAHSIAVYLSSDGEIDTNPLITWLWAQGKQVALPVIHPF
SKGHLLFLEYTENSPLVHNKYQILEPKLNKTTIIPTSELDLILTPLVAFDHTGNRLGMGGGYYDRTLSNWHSAQQGPMPI
GLSHTCQQVSSLPIESWDVPLPQIITPQKTWHW
>Mature_192_residues
TSRRLIRQSTRQRRNALSPIEQSQAANALVQQCSINPVIQKAHSIAVYLSSDGEIDTNPLITWLWAQGKQVALPVIHPFS
KGHLLFLEYTENSPLVHNKYQILEPKLNKTTIIPTSELDLILTPLVAFDHTGNRLGMGGGYYDRTLSNWHSAQQGPMPIG
LSHTCQQVSSLPIESWDVPLPQIITPQKTWHW

Specific function: Unknown

COG id: COG0212

COG function: function code H; 5-formyltetrahydrofolate cyclo-ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5-formyltetrahydrofolate cyclo-ligase family [H]

Homologues:

Organism=Homo sapiens, GI5453746, Length=190, Percent_Identity=27.8947368421053, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1789278, Length=182, Percent_Identity=52.1978021978022, Blast_Score=207, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI17510857, Length=193, Percent_Identity=30.5699481865285, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321031, Length=214, Percent_Identity=28.5046728971963, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI161077430, Length=198, Percent_Identity=30.3030303030303, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002698 [H]

Pfam domain/function: PF01812 5-FTHF_cyc-lig [H]

EC number: 6.3.3.2

Molecular weight: Translated: 21734; Mature: 21603

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRRLIRQSTRQRRNALSPIEQSQAANALVQQCSINPVIQKAHSIAVYLSSDGEIDTNP
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHCCCEEEEEECCCCCCCCCC
LITWLWAQGKQVALPVIHPFSKGHLLFLEYTENSPLVHNKYQILEPKLNKTTIIPTSELD
EEEEEECCCCEEEEEEECCCCCCCEEEEEECCCCCEEECCCEEECCCCCCEEEECCCCCH
LILTPLVAFDHTGNRLGMGGGYYDRTLSNWHSAQQGPMPIGLSHTCQQVSSLPIESWDVP
HHHHHHHHCCCCCCEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
LPQIITPQKTWHW
CCCCCCCCCCCCC
>Mature Secondary Structure 
TSRRLIRQSTRQRRNALSPIEQSQAANALVQQCSINPVIQKAHSIAVYLSSDGEIDTNP
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHCCCEEEEEECCCCCCCCCC
LITWLWAQGKQVALPVIHPFSKGHLLFLEYTENSPLVHNKYQILEPKLNKTTIIPTSELD
EEEEEECCCCEEEEEEECCCCCCCEEEEEECCCCCEEECCCEEECCCCCCEEEECCCCCH
LILTPLVAFDHTGNRLGMGGGYYDRTLSNWHSAQQGPMPIGLSHTCQQVSSLPIESWDVP
HHHHHHHHCCCCCCEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
LPQIITPQKTWHW
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 5-formyltetrahydrofolate

Specific reaction: ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]