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The map label for this gene is rpoE [H]

Identifier: 59712700

GI number: 59712700

Start: 2330081

End: 2330659

Strand: Reverse

Name: rpoE [H]

Synonym: VF_2093

Alternate gene names: 59712700

Gene position: 2330659-2330081 (Counterclockwise)

Preceding gene: 59712702

Following gene: 59712699

Centisome position: 80.44

GC content: 41.62

Gene sequence:

>579_bases
ATGAGTGAGCAGTTAACAGATCAAGTTTTAATTGAGCGTGTTCAACGTGGCGATAAACAAGCATTTAATCATCTTGTTGT
AAAATATCAAAACAAAGTATGTAACCTTGTTGCTCGTTACGTAAAGAACAGTGGTGATGTGCCTGATGTAGCCCAGGAAG
CGTTTATTAAAGCGTATCGTGCGTTACCAACCTTTCGTGGGGACAGTGCATTTTATACTTGGTTGTATCGCATAGCAGTC
AATACAGCTAAGAATTACCTTGTTGCTCAAGGACGCAGGCCGCCAGCATCAGATGTTGATGCAGAAGAGGCGGAATATTA
TGAAGGGGCGAGTGCATTAAAGGAAATCACGAACCCAGAGAATTTATCGTTGTCAGAAGAGTTAAGAGAGATCGTCTTTT
CAACAATTGATGGATTGCCAGAAGATTTAAAAACAGCAATTACATTACGCGAGCTAGAGGGCTTAAGTTATGAAGAAATT
GCTGCTGTAATGGAGTGTCCGGTTGGTACGGTTCGTTCGCGTATTTTCCGAGCTCGTGAAGTTGTTGAGAAAAAAATCCA
ACCGCTAATGCAGCGTTAA

Upstream 100 bases:

>100_bases
GTGCTCAAGTTAATCGTGGTATAGCTGGTTCATTATAAGCTATTGCGTTATGTAGACTTAATGCGCATCTAAGAAGAATA
ACATGGGAGTACCCGCTCCA

Downstream 100 bases:

>100_bases
TTATTTCCAACGGTCAAACGGTGAAGACAATGACTGATAAAGAACAAATATCTGCGTTTATAGATAATGAAGAGATGGAC
AACGCGTTCATTGAATCCTT

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MSEQLTDQVLIERVQRGDKQAFNHLVVKYQNKVCNLVARYVKNSGDVPDVAQEAFIKAYRALPTFRGDSAFYTWLYRIAV
NTAKNYLVAQGRRPPASDVDAEEAEYYEGASALKEITNPENLSLSEELREIVFSTIDGLPEDLKTAITLRELEGLSYEEI
AAVMECPVGTVRSRIFRAREVVEKKIQPLMQR

Sequences:

>Translated_192_residues
MSEQLTDQVLIERVQRGDKQAFNHLVVKYQNKVCNLVARYVKNSGDVPDVAQEAFIKAYRALPTFRGDSAFYTWLYRIAV
NTAKNYLVAQGRRPPASDVDAEEAEYYEGASALKEITNPENLSLSEELREIVFSTIDGLPEDLKTAITLRELEGLSYEEI
AAVMECPVGTVRSRIFRAREVVEKKIQPLMQR
>Mature_191_residues
SEQLTDQVLIERVQRGDKQAFNHLVVKYQNKVCNLVARYVKNSGDVPDVAQEAFIKAYRALPTFRGDSAFYTWLYRIAVN
TAKNYLVAQGRRPPASDVDAEEAEYYEGASALKEITNPENLSLSEELREIVFSTIDGLPEDLKTAITLRELEGLSYEEIA
AVMECPVGTVRSRIFRAREVVEKKIQPLMQR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=192, Percent_Identity=79.1666666666667, Blast_Score=317, Evalue=2e-88,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21806; Mature: 21675

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQLTDQVLIERVQRGDKQAFNHLVVKYQNKVCNLVARYVKNSGDVPDVAQEAFIKAYR
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ALPTFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPASDVDAEEAEYYEGASALKEITNPE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
NLSLSEELREIVFSTIDGLPEDLKTAITLRELEGLSYEEIAAVMECPVGTVRSRIFRARE
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHH
VVEKKIQPLMQR
HHHHHHHHHHCC
>Mature Secondary Structure 
SEQLTDQVLIERVQRGDKQAFNHLVVKYQNKVCNLVARYVKNSGDVPDVAQEAFIKAYR
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ALPTFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPASDVDAEEAEYYEGASALKEITNPE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
NLSLSEELREIVFSTIDGLPEDLKTAITLRELEGLSYEEIAAVMECPVGTVRSRIFRARE
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHH
VVEKKIQPLMQR
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]