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The map label for this gene is pdxJ

Identifier: 59712691

GI number: 59712691

Start: 2321831

End: 2322562

Strand: Reverse

Name: pdxJ

Synonym: VF_2084

Alternate gene names: 59712691

Gene position: 2322562-2321831 (Counterclockwise)

Preceding gene: 59712692

Following gene: 59712690

Centisome position: 80.16

GC content: 43.44

Gene sequence:

>732_bases
ATGAGTTCAATTTTACTTGGCGTTAATATCGATCACGTAGCAACCCTTCGAAATGCGCGAGGTACTAAATACCCAGATCC
TGTACACGCTGCAGAAATTGCTGAGCGTGCTGGTGCTGCCGGTATTACGATTCACCTACGTGAAGATCGCCGTCATATTA
AAGATCGAGATGTACGTATTCTACGTGAAACGCTGCAAACTCGAATGAATTTAGAAATGGCTGTTACAGATGAAATGGTT
GGGATCGCACTAGAAACCAAACCTGAATTTGTTTGCCTTGTTCCTGAAAAACGCGAAGAGTTAACAACAGAAGGCGGCCT
GAATGTGTCAGGTCAGCTTGAAAAAGTGAAAGCTGCAACTCAAAAGCTAACAGAAGCGGGCATTAAGGTATCCTTGTTTA
TTGATGCTGATAAAGAGCAAATTGATGCGGCTGTTGAATGTGGTGCACCTTTCATTGAGCTTCATACCGGCGCTTATGCA
GATGCAGAGACAGAAGAAGCACAGCAAGATGAGCTGAAGAAGATTGCAGCTGGTGCAAGCTATGCAGCAAGTAAAGGGCT
TATTGTAAATGCTGGTCATGGTTTGACTTATCATAATGTAGAAGCCATCGCAGCATTGCCAGAGATCTACGAACTAAACA
TTGGTCACTCAATTATGGGACGAGCGATGTTTGATGGTTTAGAAAAAGCAGTCGCTGATATGCATCGTATTATGTTAGGT
GCTCGCAAGTAA

Upstream 100 bases:

>100_bases
CGGCAAAACGCTTTACACGTATAGCCTTAAAGCCGTATCTTGGCGGTAAGCCCCTAAAAAGTAGGGAACTGTTTCTTCCT
AGAACAAGGAGTATTCTAAA

Downstream 100 bases:

>100_bases
TGGCAATTGTTGGTTTAGGTACTGACATAGCAGAGATTGAAAGGGTTGAAAAAGCCCTTTCACGTTCAGGAGATGCGTTT
GCAGAGCGCATTTTAAGTCA

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSSILLGVNIDHVATLRNARGTKYPDPVHAAEIAERAGAAGITIHLREDRRHIKDRDVRILRETLQTRMNLEMAVTDEMV
GIALETKPEFVCLVPEKREELTTEGGLNVSGQLEKVKAATQKLTEAGIKVSLFIDADKEQIDAAVECGAPFIELHTGAYA
DAETEEAQQDELKKIAAGASYAASKGLIVNAGHGLTYHNVEAIAALPEIYELNIGHSIMGRAMFDGLEKAVADMHRIMLG
ARK

Sequences:

>Translated_243_residues
MSSILLGVNIDHVATLRNARGTKYPDPVHAAEIAERAGAAGITIHLREDRRHIKDRDVRILRETLQTRMNLEMAVTDEMV
GIALETKPEFVCLVPEKREELTTEGGLNVSGQLEKVKAATQKLTEAGIKVSLFIDADKEQIDAAVECGAPFIELHTGAYA
DAETEEAQQDELKKIAAGASYAASKGLIVNAGHGLTYHNVEAIAALPEIYELNIGHSIMGRAMFDGLEKAVADMHRIMLG
ARK
>Mature_242_residues
SSILLGVNIDHVATLRNARGTKYPDPVHAAEIAERAGAAGITIHLREDRRHIKDRDVRILRETLQTRMNLEMAVTDEMVG
IALETKPEFVCLVPEKREELTTEGGLNVSGQLEKVKAATQKLTEAGIKVSLFIDADKEQIDAAVECGAPFIELHTGAYAD
AETEEAQQDELKKIAAGASYAASKGLIVNAGHGLTYHNVEAIAALPEIYELNIGHSIMGRAMFDGLEKAVADMHRIMLGA
RK

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=242, Percent_Identity=71.4876033057851, Blast_Score=360, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_VIBF1 (Q5E317)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205467.1
- ProteinModelPortal:   Q5E317
- SMR:   Q5E317
- STRING:   Q5E317
- GeneID:   3279332
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2084
- NMPDR:   fig|312309.3.peg.2084
- eggNOG:   COG0854
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- PhylomeDB:   Q5E317
- ProtClustDB:   PRK05265
- BioCyc:   VFIS312309:VF2084-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 26421; Mature: 26289

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: ACT_SITE 45-45 ACT_SITE 72-72 ACT_SITE 193-193 BINDING 9-9 BINDING 20-20 BINDING 47-47 BINDING 52-52 BINDING 102-102 BINDING 194-194

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSILLGVNIDHVATLRNARGTKYPDPVHAAEIAERAGAAGITIHLREDRRHIKDRDVRI
CCCEEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHH
LRETLQTRMNLEMAVTDEMVGIALETKPEFVCLVPEKREELTTEGGLNVSGQLEKVKAAT
HHHHHHHHHCCEEEEECHHEEEEEECCCCEEEECCCHHHHHHCCCCCCCCCHHHHHHHHH
QKLTEAGIKVSLFIDADKEQIDAAVECGAPFIELHTGAYADAETEEAQQDELKKIAAGAS
HHHHHCCCEEEEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
YAASKGLIVNAGHGLTYHNVEAIAALPEIYELNIGHSIMGRAMFDGLEKAVADMHRIMLG
HHCCCCEEEECCCCCEECCHHHHHHCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHC
ARK
CCC
>Mature Secondary Structure 
SSILLGVNIDHVATLRNARGTKYPDPVHAAEIAERAGAAGITIHLREDRRHIKDRDVRI
CCEEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHH
LRETLQTRMNLEMAVTDEMVGIALETKPEFVCLVPEKREELTTEGGLNVSGQLEKVKAAT
HHHHHHHHHCCEEEEECHHEEEEEECCCCEEEECCCHHHHHHCCCCCCCCCHHHHHHHHH
QKLTEAGIKVSLFIDADKEQIDAAVECGAPFIELHTGAYADAETEEAQQDELKKIAAGAS
HHHHHCCCEEEEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
YAASKGLIVNAGHGLTYHNVEAIAALPEIYELNIGHSIMGRAMFDGLEKAVADMHRIMLG
HHCCCCEEEECCCCCEECCHHHHHHCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHC
ARK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA