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The map label for this gene is yafJ [H]

Identifier: 59712537

GI number: 59712537

Start: 2159004

End: 2159837

Strand: Reverse

Name: yafJ [H]

Synonym: VF_1930

Alternate gene names: 59712537

Gene position: 2159837-2159004 (Counterclockwise)

Preceding gene: 59712538

Following gene: 172087735

Centisome position: 74.54

GC content: 40.89

Gene sequence:

>834_bases
ATGTGTGAACTGCTCGGAATGAGTGCTAATGTACCAACAGATATTTGTTTTAGTTTTAAAGGATTAGTTCAGCGTGGTGG
TAATACAGGACCACACCGTGATGGTTGGGGAATTACTTTTTATGAAGGGAAAGGATTTCGTACATTTAAAGACCCTACGC
CAAGTTGTCATTCAAAAATAGCTGAACTTGTTCAAGATTACCCTATTAAGAGTAAAGCGGTAGTCAGTCACATTAGGCAA
GCGAACCGAGGTGATGTAAACCTTGAAAATACACACCCTTTTACTCGTGAGTTATGGGGGAAGTATTGGACGTTTGCTCA
CAATGGTCAACTAACGGGGTTCGACCAATTATCTACAGGGCGTTTTCGACCTGTTGGTGAGACAGACAGTGAACGTGCTT
TTTGTTGGTTATTAAATCAATTAGAAGATAAGTATCCTGAACCTCCTCAAGATATGATGCTGATGTTTGAGTTTGTTTCT
GAGTGTAGTGATCATTTAAGAAGCTTAGGGGTATTCAATATGCTATTAAGTGATGGTGAGTATGTGATGACTTACTGTTC
AAATAATTTGCATTGGATAACTCGAAGAGCACCTTTCGGTCAAGCCTCCTTAATTGATGAAGACGTAACGATTGATTTTC
ATAAAGAGACAACGCCTAATGATGTTGTTTCGATTATTGCAACGCAGCCTCTAACGGATAACGAAGAGTGGCGTAAAATG
AAAGTCGGTGAGTACGCGTTGTTTCACTTTGGAGAGCTGAGTGCAGGAAACCACGATACGGTTCCTGACATGGCACCACC
TCCATCTGAGTGTCCAGTAAATAGTGCTCTTTAG

Upstream 100 bases:

>100_bases
ATTGATTGAAAAAGAGATGGAAGCATAATCTATCTTGCAGCCTATCTTGATATTTATATATTGAGATAGGCTATTTTGCG
CATAAAGGAGTAACGCAAGT

Downstream 100 bases:

>100_bases
CATTAAAAGTACATAGAACTTAAAAAGCCGCTTAGTTATTTAAGCGGCTTTTTTGTATCTATTCTGTCTCTAAACCGGAT
AGACCTAATTCGGTACCATC

Product: amidotransfease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MCELLGMSANVPTDICFSFKGLVQRGGNTGPHRDGWGITFYEGKGFRTFKDPTPSCHSKIAELVQDYPIKSKAVVSHIRQ
ANRGDVNLENTHPFTRELWGKYWTFAHNGQLTGFDQLSTGRFRPVGETDSERAFCWLLNQLEDKYPEPPQDMMLMFEFVS
ECSDHLRSLGVFNMLLSDGEYVMTYCSNNLHWITRRAPFGQASLIDEDVTIDFHKETTPNDVVSIIATQPLTDNEEWRKM
KVGEYALFHFGELSAGNHDTVPDMAPPPSECPVNSAL

Sequences:

>Translated_277_residues
MCELLGMSANVPTDICFSFKGLVQRGGNTGPHRDGWGITFYEGKGFRTFKDPTPSCHSKIAELVQDYPIKSKAVVSHIRQ
ANRGDVNLENTHPFTRELWGKYWTFAHNGQLTGFDQLSTGRFRPVGETDSERAFCWLLNQLEDKYPEPPQDMMLMFEFVS
ECSDHLRSLGVFNMLLSDGEYVMTYCSNNLHWITRRAPFGQASLIDEDVTIDFHKETTPNDVVSIIATQPLTDNEEWRKM
KVGEYALFHFGELSAGNHDTVPDMAPPPSECPVNSAL
>Mature_277_residues
MCELLGMSANVPTDICFSFKGLVQRGGNTGPHRDGWGITFYEGKGFRTFKDPTPSCHSKIAELVQDYPIKSKAVVSHIRQ
ANRGDVNLENTHPFTRELWGKYWTFAHNGQLTGFDQLSTGRFRPVGETDSERAFCWLLNQLEDKYPEPPQDMMLMFEFVS
ECSDHLRSLGVFNMLLSDGEYVMTYCSNNLHWITRRAPFGQASLIDEDVTIDFHKETTPNDVVSIIATQPLTDNEEWRKM
KVGEYALFHFGELSAGNHDTVPDMAPPPSECPVNSAL

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=252, Percent_Identity=73.015873015873, Blast_Score=401, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 31336; Mature: 31336

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCELLGMSANVPTDICFSFKGLVQRGGNTGPHRDGWGITFYEGKGFRTFKDPTPSCHSKI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHH
AELVQDYPIKSKAVVSHIRQANRGDVNLENTHPFTRELWGKYWTFAHNGQLTGFDQLSTG
HHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCC
RFRPVGETDSERAFCWLLNQLEDKYPEPPQDMMLMFEFVSECSDHLRSLGVFNMLLSDGE
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YVMTYCSNNLHWITRRAPFGQASLIDEDVTIDFHKETTPNDVVSIIATQPLTDNEEWRKM
EEEEEECCCEEEEEECCCCCCHHHCCCCCEEEECCCCCHHHHEEHHCCCCCCCCHHHHHH
KVGEYALFHFGELSAGNHDTVPDMAPPPSECPVNSAL
HCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCELLGMSANVPTDICFSFKGLVQRGGNTGPHRDGWGITFYEGKGFRTFKDPTPSCHSKI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHH
AELVQDYPIKSKAVVSHIRQANRGDVNLENTHPFTRELWGKYWTFAHNGQLTGFDQLSTG
HHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCC
RFRPVGETDSERAFCWLLNQLEDKYPEPPQDMMLMFEFVSECSDHLRSLGVFNMLLSDGE
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YVMTYCSNNLHWITRRAPFGQASLIDEDVTIDFHKETTPNDVVSIIATQPLTDNEEWRKM
EEEEEECCCEEEEEECCCCCCHHHCCCCCEEEECCCCCHHHHEEHHCCCCCCCCHHHHHH
KVGEYALFHFGELSAGNHDTVPDMAPPPSECPVNSAL
HCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]