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The map label for this gene is dapE

Identifier: 59712521

GI number: 59712521

Start: 2144250

End: 2145383

Strand: Direct

Name: dapE

Synonym: VF_1914

Alternate gene names: 59712521

Gene position: 2144250-2145383 (Clockwise)

Preceding gene: 59712520

Following gene: 59712522

Centisome position: 74.0

GC content: 40.92

Gene sequence:

>1134_bases
ATGTCTGATACACCAACATTAGCTCTTGCTAAAGATTTACTAAGCCGCCAATCCATCACACCTGAAGATGCAGGTTGCCA
AGAGCTAATGATTAAACAACTAGAAGCACTTGGTTTTACCATTGAAATTATGGTGTTTGAAGATACAACCAACTTTTGGG
CTCGTCGAGGCAATAAAGCACCATTATTTACTTTTGCAGGACATACGGATGTTGTTCCTACAGGTGATTTAACGCATTGG
AATACCAATCCATTTGAGCCAACAATCATTGATGGAATGCTCTACGCTCGTGGAGCAGCTGATATGAAGGGCTCTTTAGC
TTGTATGGTGGTTGCCGTTGAGCGTTTCGTCGCAGAGCACCCTAATCACAAAGGTTCAATTTCATTTTTAATCACATCCG
ATGAAGAAGGTCCATTTATTAATGGCACGACTCGTGTAGTGGATACCCTGCAAGAACGTAACGAAATCATTGATATGTGT
ATCGTAGGCGAACCATCAAGTACCGCTCATGTTGGTGATGTGGTTAAAAATGGACGTAGAGGCTCATTAACAGGTAATTT
AACCGTTAAAGGGATTCAAGGGCATGTTGCGTATCCGCATATAGCTCGCAATCCAATTCACCAAGCGATGCCCGCTCTGT
CAGAGCTAGCAACGACCGTTTGGGACAATGGCAATGATTATTTTCCACCAACAAGCTTTCAAATCCCAAATATGAATGGT
GGCACAGGCGCTTCTAACGTTATTCCTGGCACAGTTGATATCATGTTTAACTTTAGATTCAGTACAGAATCAACAGTAGA
TGAGCTACAACAACGTGTCGTCGAAATTTTAGATAAGCATGATTTAGAATACGAACTAGATTGGATTATCAATGGCCTCC
CTTTCCTAACTGATACTGGGGATTTATTAACAGCCGTTGTTAATGCTGTCGATACGGTTAATCAACAAAAACCACAATTA
TTAACGACAGGTGGTACGTCAGATGGGCGTTTTATTGCTCAAATGGGTTCCCAAGTTATAGAGCTTGGCCCTGTGAATGC
GACCATCCATAAAGTAAATGAGTGCGTAAATGTAGAAGATTTAGAAAAGCTAACTGATATGTATCAAGAAGTCCTAAATA
ATCTACTAGCATAA

Upstream 100 bases:

>100_bases
TATTCATAATAATGAATATCACTTAGGCTTTAAGCCAGCTCAATATGAGACCTTGTTTGATATCTAATGCCATTAATAAA
AAGAAGTAAAGGAATACAGA

Downstream 100 bases:

>100_bases
GGATAAATTAGGGTTATGATGAGAGAGCTTTTGTTCTCTCATCTCTAATTGGTGCCCTATGACATATCAAGAACTTACTG
GTCAATCATTTCAACACTTG

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSDTPTLALAKDLLSRQSITPEDAGCQELMIKQLEALGFTIEIMVFEDTTNFWARRGNKAPLFTFAGHTDVVPTGDLTHW
NTNPFEPTIIDGMLYARGAADMKGSLACMVVAVERFVAEHPNHKGSISFLITSDEEGPFINGTTRVVDTLQERNEIIDMC
IVGEPSSTAHVGDVVKNGRRGSLTGNLTVKGIQGHVAYPHIARNPIHQAMPALSELATTVWDNGNDYFPPTSFQIPNMNG
GTGASNVIPGTVDIMFNFRFSTESTVDELQQRVVEILDKHDLEYELDWIINGLPFLTDTGDLLTAVVNAVDTVNQQKPQL
LTTGGTSDGRFIAQMGSQVIELGPVNATIHKVNECVNVEDLEKLTDMYQEVLNNLLA

Sequences:

>Translated_377_residues
MSDTPTLALAKDLLSRQSITPEDAGCQELMIKQLEALGFTIEIMVFEDTTNFWARRGNKAPLFTFAGHTDVVPTGDLTHW
NTNPFEPTIIDGMLYARGAADMKGSLACMVVAVERFVAEHPNHKGSISFLITSDEEGPFINGTTRVVDTLQERNEIIDMC
IVGEPSSTAHVGDVVKNGRRGSLTGNLTVKGIQGHVAYPHIARNPIHQAMPALSELATTVWDNGNDYFPPTSFQIPNMNG
GTGASNVIPGTVDIMFNFRFSTESTVDELQQRVVEILDKHDLEYELDWIINGLPFLTDTGDLLTAVVNAVDTVNQQKPQL
LTTGGTSDGRFIAQMGSQVIELGPVNATIHKVNECVNVEDLEKLTDMYQEVLNNLLA
>Mature_376_residues
SDTPTLALAKDLLSRQSITPEDAGCQELMIKQLEALGFTIEIMVFEDTTNFWARRGNKAPLFTFAGHTDVVPTGDLTHWN
TNPFEPTIIDGMLYARGAADMKGSLACMVVAVERFVAEHPNHKGSISFLITSDEEGPFINGTTRVVDTLQERNEIIDMCI
VGEPSSTAHVGDVVKNGRRGSLTGNLTVKGIQGHVAYPHIARNPIHQAMPALSELATTVWDNGNDYFPPTSFQIPNMNGG
TGASNVIPGTVDIMFNFRFSTESTVDELQQRVVEILDKHDLEYELDWIINGLPFLTDTGDLLTAVVNAVDTVNQQKPQLL
TTGGTSDGRFIAQMGSQVIELGPVNATIHKVNECVNVEDLEKLTDMYQEVLNNLLA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=373, Percent_Identity=62.1983914209115, Blast_Score=492, Evalue=1e-140,
Organism=Escherichia coli, GI1790395, Length=276, Percent_Identity=27.536231884058, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_VIBF1 (Q5E3I7)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205297.1
- ProteinModelPortal:   Q5E3I7
- SMR:   Q5E3I7
- STRING:   Q5E3I7
- MEROPS:   M20.010
- GeneID:   3279061
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1914
- NMPDR:   fig|312309.3.peg.1914
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- PhylomeDB:   Q5E3I7
- ProtClustDB:   PRK13009
- BioCyc:   VFIS312309:VF1914-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41245; Mature: 41114

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 70-70 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTPTLALAKDLLSRQSITPEDAGCQELMIKQLEALGFTIEIMVFEDTTNFWARRGNKA
CCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEEEEEECCHHHHHHCCCCC
PLFTFAGHTDVVPTGDLTHWNTNPFEPTIIDGMLYARGAADMKGSLACMVVAVERFVAEH
CEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHC
PNHKGSISFLITSDEEGPFINGTTRVVDTLQERNEIIDMCIVGEPSSTAHVGDVVKNGRR
CCCCCCEEEEEECCCCCCEECCHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHCCCC
GSLTGNLTVKGIQGHVAYPHIARNPIHQAMPALSELATTVWDNGNDYFPPTSFQIPNMNG
CCEEECEEEEEECCCEECCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCC
GTGASNVIPGTVDIMFNFRFSTESTVDELQQRVVEILDKHDLEYELDWIINGLPFLTDTG
CCCCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCH
DLLTAVVNAVDTVNQQKPQLLTTGGTSDGRFIAQMGSQVIELGPVNATIHKVNECVNVED
HHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHCCHHEECCCCCHHHHHHHHHCCHHH
LEKLTDMYQEVLNNLLA
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDTPTLALAKDLLSRQSITPEDAGCQELMIKQLEALGFTIEIMVFEDTTNFWARRGNKA
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEEEEEECCHHHHHHCCCCC
PLFTFAGHTDVVPTGDLTHWNTNPFEPTIIDGMLYARGAADMKGSLACMVVAVERFVAEH
CEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHC
PNHKGSISFLITSDEEGPFINGTTRVVDTLQERNEIIDMCIVGEPSSTAHVGDVVKNGRR
CCCCCCEEEEEECCCCCCEECCHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHCCCC
GSLTGNLTVKGIQGHVAYPHIARNPIHQAMPALSELATTVWDNGNDYFPPTSFQIPNMNG
CCEEECEEEEEECCCEECCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCC
GTGASNVIPGTVDIMFNFRFSTESTVDELQQRVVEILDKHDLEYELDWIINGLPFLTDTG
CCCCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCH
DLLTAVVNAVDTVNQQKPQLLTTGGTSDGRFIAQMGSQVIELGPVNATIHKVNECVNVED
HHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHCCHHEECCCCCHHHHHHHHHCCHHH
LEKLTDMYQEVLNNLLA
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA