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The map label for this gene is gltX [H]

Identifier: 59712494

GI number: 59712494

Start: 2120518

End: 2121942

Strand: Reverse

Name: gltX [H]

Synonym: VF_1887

Alternate gene names: 59712494

Gene position: 2121942-2120518 (Counterclockwise)

Preceding gene: 59712495

Following gene: 59712491

Centisome position: 73.23

GC content: 41.26

Gene sequence:

>1425_bases
ATGACGGTTAAAACTCGTTTTGCTCCAAGCCCAACAGGCTATCTTCACGTTGGTGGTGCTCGTACAGCACTTTACTCATG
GCTTTTTGCTAAAAACCAAGGTGGCGAATTTGTTCTACGTATCGAAGATACGGATCTAGAGCGTAACTCTCAAGAAGCGG
TAGACGCTATTATTGAAGGTATGCACTGGATGGGTATGGAATGGGATGAAGGTCCATATTACCAATCTAAGCGTTTTGAC
CGTTACAATGAAGTTGTTGACCAACTGCTTGCTGAAGATAAAGCATACAAATGTTATGCATCAAAAGAACTGCTAGATGA
AATTCGTGCAGAGCAAGAAGCAAACAAAGAAATGGCTCGTTACGATGCAAACCATCCTAAAATTGTTGCTGCGAATGCGG
CTGCAAAAGAAGGTGATGCATGTGTAATTCGCTTCCGTAACCCAAAAGAAGGTTCGGTAGTATTTGATGACCAAATTCGT
GGCCGCATCGAAATCTCAAATAGCCAGTTAGATGACTTAATCATTCGTCGTACTGATGGTGCTCCAACATACAACTTCGT
AGTAGTGGTTGATGACTGGGATATGGGTATTACTCAAGTTATTCGTGGTGAAGACCATATCAATAACACGCCTCGTCAAA
TCAATATTTATGAAGCATTAGGTGCTCCTGTACCTATGTTTGCACACTGTGCAATGATCCTTGGCGATGATGGCGCAAAA
CTGTCTAAGCGTCATGGTGCGGTATCTGTAATGCAATACCGTGATGAAGGTTACCTACCAAACGCACTGAATAACTACTT
AGTACGTTTAGGTTGGTCTCATGGTGATCAAGAGATTTTCTCTCAAGAAGAGATGATTAATCTATTTAGTTTAAGCGCAG
TAAGTAAATCAGCATCAGCATTCAACACTGATAAATTGTTATGGTTAAACAACCACTACATTAAATCATCAGAGCCTGAG
TATGTTGCAAAATACCTACAATGGCACTTAGATCAAAAAGAAATCTCTTTAGATAATGGTCCTGCAATTACTGAAGTTAT
TAAGCTAGTGGGTGAGCGTTGCAATACGCTGATCGAATTAGCTGATCAATCTCGTTATTTCTACCAAGATTTCGAAGAGT
TTGAAGCTGGTGCAGCTAAAAAACACTTACGTGGTGTTGCTAAAGGCCCATTAGAGCTTGCTTTAGCTAAAGTGGAAGCG
CTAGAAGAGTGGACTACTGAAAATCTTCACAATGTGATTGAAGAAGTGTGTGCTGAGTTAGAGATCGGCATGGGCAAAAT
TGGTATGCCTCTACGTGTTGCAGTAACTGGCGGTGGTCAATCACCATCAGTAGATGCTGTTATGCAATTAGTTGGTAAAG
AGCGTGTTGTTGCTCGCATCAAGATGGCATTAGCATTTATTGCAGAGCGTGAAGCTAACGCATAA

Upstream 100 bases:

>100_bases
CTCTCTTTCCCATTGTAGAATTACCTTATAGTGCTAAAATAGCGCACAAATTATGTGGTGCTGACAGATTCAGCTCAACC
TCTTCAAAAAGGTAATAACA

Downstream 100 bases:

>100_bases
TCCTGCTTATATTTTGGATAAAAACCGCTGTTATTCAGCGGTTTTTTTATGCTTTGCGTTTTTACTTTTTCAGTAATTAC
TTAGTCATTGCTATTGATTA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTVKTRFAPSPTGYLHVGGARTALYSWLFAKNQGGEFVLRIEDTDLERNSQEAVDAIIEGMHWMGMEWDEGPYYQSKRFD
RYNEVVDQLLAEDKAYKCYASKELLDEIRAEQEANKEMARYDANHPKIVAANAAAKEGDACVIRFRNPKEGSVVFDDQIR
GRIEISNSQLDDLIIRRTDGAPTYNFVVVVDDWDMGITQVIRGEDHINNTPRQINIYEALGAPVPMFAHCAMILGDDGAK
LSKRHGAVSVMQYRDEGYLPNALNNYLVRLGWSHGDQEIFSQEEMINLFSLSAVSKSASAFNTDKLLWLNNHYIKSSEPE
YVAKYLQWHLDQKEISLDNGPAITEVIKLVGERCNTLIELADQSRYFYQDFEEFEAGAAKKHLRGVAKGPLELALAKVEA
LEEWTTENLHNVIEEVCAELEIGMGKIGMPLRVAVTGGGQSPSVDAVMQLVGKERVVARIKMALAFIAEREANA

Sequences:

>Translated_474_residues
MTVKTRFAPSPTGYLHVGGARTALYSWLFAKNQGGEFVLRIEDTDLERNSQEAVDAIIEGMHWMGMEWDEGPYYQSKRFD
RYNEVVDQLLAEDKAYKCYASKELLDEIRAEQEANKEMARYDANHPKIVAANAAAKEGDACVIRFRNPKEGSVVFDDQIR
GRIEISNSQLDDLIIRRTDGAPTYNFVVVVDDWDMGITQVIRGEDHINNTPRQINIYEALGAPVPMFAHCAMILGDDGAK
LSKRHGAVSVMQYRDEGYLPNALNNYLVRLGWSHGDQEIFSQEEMINLFSLSAVSKSASAFNTDKLLWLNNHYIKSSEPE
YVAKYLQWHLDQKEISLDNGPAITEVIKLVGERCNTLIELADQSRYFYQDFEEFEAGAAKKHLRGVAKGPLELALAKVEA
LEEWTTENLHNVIEEVCAELEIGMGKIGMPLRVAVTGGGQSPSVDAVMQLVGKERVVARIKMALAFIAEREANA
>Mature_473_residues
TVKTRFAPSPTGYLHVGGARTALYSWLFAKNQGGEFVLRIEDTDLERNSQEAVDAIIEGMHWMGMEWDEGPYYQSKRFDR
YNEVVDQLLAEDKAYKCYASKELLDEIRAEQEANKEMARYDANHPKIVAANAAAKEGDACVIRFRNPKEGSVVFDDQIRG
RIEISNSQLDDLIIRRTDGAPTYNFVVVVDDWDMGITQVIRGEDHINNTPRQINIYEALGAPVPMFAHCAMILGDDGAKL
SKRHGAVSVMQYRDEGYLPNALNNYLVRLGWSHGDQEIFSQEEMINLFSLSAVSKSASAFNTDKLLWLNNHYIKSSEPEY
VAKYLQWHLDQKEISLDNGPAITEVIKLVGERCNTLIELADQSRYFYQDFEEFEAGAAKKHLRGVAKGPLELALAKVEAL
EEWTTENLHNVIEEVCAELEIGMGKIGMPLRVAVTGGGQSPSVDAVMQLVGKERVVARIKMALAFIAEREANA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=329, Percent_Identity=35.5623100303951, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI62241042, Length=364, Percent_Identity=24.1758241758242, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1788743, Length=471, Percent_Identity=68.1528662420382, Blast_Score=693, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=304, Percent_Identity=32.8947368421053, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1786895, Length=214, Percent_Identity=26.6355140186916, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=483, Percent_Identity=30.0207039337474, Blast_Score=201, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6324540, Length=495, Percent_Identity=31.5151515151515, Blast_Score=235, Evalue=9e-63,
Organism=Saccharomyces cerevisiae, GI6321192, Length=214, Percent_Identity=27.5700934579439, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324742, Length=125, Percent_Identity=30.4, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24665321, Length=492, Percent_Identity=30.6910569105691, Blast_Score=226, Evalue=4e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 53250; Mature: 53119

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKTRFAPSPTGYLHVGGARTALYSWLFAKNQGGEFVLRIEDTDLERNSQEAVDAIIEG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHC
MHWMGMEWDEGPYYQSKRFDRYNEVVDQLLAEDKAYKCYASKELLDEIRAEQEANKEMAR
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YDANHPKIVAANAAAKEGDACVIRFRNPKEGSVVFDDQIRGRIEISNSQLDDLIIRRTDG
HCCCCCEEEEECCCCCCCCEEEEEECCCCCCCEEECCCCCCEEEECCCCCCCEEEEECCC
APTYNFVVVVDDWDMGITQVIRGEDHINNTPRQINIYEALGAPVPMFAHCAMILGDDGAK
CCCEEEEEEEECCCCCHHHHHCCCHHCCCCCCEEEEHHHHCCCCCHHHHHHHHCCCCCCH
LSKRHGAVSVMQYRDEGYLPNALNNYLVRLGWSHGDQEIFSQEEMINLFSLSAVSKSASA
HHHHCCCEEEEEECCCCCCCHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
FNTDKLLWLNNHYIKSSEPEYVAKYLQWHLDQKEISLDNGPAITEVIKLVGERCNTLIEL
CCCCEEEEEECCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
ADQSRYFYQDFEEFEAGAAKKHLRGVAKGPLELALAKVEALEEWTTENLHNVIEEVCAEL
HHCCHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGMGKIGMPLRVAVTGGGQSPSVDAVMQLVGKERVVARIKMALAFIAEREANA
HCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TVKTRFAPSPTGYLHVGGARTALYSWLFAKNQGGEFVLRIEDTDLERNSQEAVDAIIEG
CCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHC
MHWMGMEWDEGPYYQSKRFDRYNEVVDQLLAEDKAYKCYASKELLDEIRAEQEANKEMAR
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YDANHPKIVAANAAAKEGDACVIRFRNPKEGSVVFDDQIRGRIEISNSQLDDLIIRRTDG
HCCCCCEEEEECCCCCCCCEEEEEECCCCCCCEEECCCCCCEEEECCCCCCCEEEEECCC
APTYNFVVVVDDWDMGITQVIRGEDHINNTPRQINIYEALGAPVPMFAHCAMILGDDGAK
CCCEEEEEEEECCCCCHHHHHCCCHHCCCCCCEEEEHHHHCCCCCHHHHHHHHCCCCCCH
LSKRHGAVSVMQYRDEGYLPNALNNYLVRLGWSHGDQEIFSQEEMINLFSLSAVSKSASA
HHHHCCCEEEEEECCCCCCCHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
FNTDKLLWLNNHYIKSSEPEYVAKYLQWHLDQKEISLDNGPAITEVIKLVGERCNTLIEL
CCCCEEEEEECCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
ADQSRYFYQDFEEFEAGAAKKHLRGVAKGPLELALAKVEALEEWTTENLHNVIEEVCAEL
HHCCHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGMGKIGMPLRVAVTGGGQSPSVDAVMQLVGKERVVARIKMALAFIAEREANA
HCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA