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The map label for this gene is flgJ [H]

Identifier: 59712476

GI number: 59712476

Start: 2104165

End: 2105184

Strand: Reverse

Name: flgJ [H]

Synonym: VF_1869

Alternate gene names: 59712476

Gene position: 2105184-2104165 (Counterclockwise)

Preceding gene: 59712477

Following gene: 59712475

Centisome position: 72.65

GC content: 38.04

Gene sequence:

>1020_bases
ATGCAAAGTGTGAAGATGAATAATTCAGTAAATATGACAGATGTGGGATTTATCCACGACGTAGGCGGTCTTGATAAATT
ACGTAAAAGTGCCGTTAGTGGCGATCAAGATAGTCAAGATAAAGCATTGCGTGCAGCAGCAGAGCAGTTTGAATCAATCT
TCACACAAATGCTTTTTAAATCAATGCGTAATGCAAATGACTCTTTTAAATCCGATATGATGAGCAGTGATAATCAGCAG
TTCTTTGAACAAATGCGTGATGATCAAATGGCCACTGAACTATCGAAAAAAGGGTCATTAGGTTTAGCTGATATGATTGT
TGCTCAATTAGGCGCTTCAATGAACCAAGCGGCGCCAGTACAAAGCGGCAATAATTTAGAGCAAGATGCAAGAATGGCAA
TGCGTAAAGCGGCAACCGATGAAACATTACGCTTGCCTGCTGATTACTCATATCCTCATGCTTCTCAAGCGAAACAAGAA
TCTCAAACAGAAGCACCTGAAATTGCACAAATTGATAAAATGTTAGCAGCGAGAAAAGTTCAACCTGCTGATAAATTTGA
AACACCTCAAGACTTTGTTAACACCATGAAGCCTTATGCAGAAAAAGCGGCAAAAGCATTAGGTGTTGATTCTAATCTTC
TTATTGCACAAGCCGCTCTTGAAACGGGTTGGGGTAAAAAGGTCATTAAGAACTCTGTAGATTCAAGTCATAACTTATTT
AATATTAAAGCAGATAGAAGTTGGCAAGGAGATAAGATCTCTAAGAACACACTTGAATATCACGATAATATTGCAGTGAC
AGAAAATGCAGCCTTCCGTTCATACGATAACTATGAAGAGAGTTTCAGTGATTTTGTTCGCTTCTTAAACCAAAATCCAC
GTTACGAACGAGCACTGCAACAAACGGATAGTTCAGAAAGCTTCATTAAAGGCATTCATAGTGCTGGTTATGCTACGGAT
CCAAATTACTCAAATAAAGTAATGAGTGTAATGAGAAAAGTAGAAAGCTTATTAAAGTAA

Upstream 100 bases:

>100_bases
TATCTAATAGCAGCTCGGCTTTACAAAGACAATAAACGCACCTCTTGTAGGTGCGTTTTTTTTGGCATTAAATTTGCTGT
AGTTATATTAGTGTTAGTTA

Downstream 100 bases:

>100_bases
TACGAACGCATTAAATACAAACACTAACATTAAATCCTGTCTCTTGAAAAAGCGGCAGGATTTTTTGTTTTTGCTTGCCG
CTTAAAATGAAAAGAGGCAA

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MQSVKMNNSVNMTDVGFIHDVGGLDKLRKSAVSGDQDSQDKALRAAAEQFESIFTQMLFKSMRNANDSFKSDMMSSDNQQ
FFEQMRDDQMATELSKKGSLGLADMIVAQLGASMNQAAPVQSGNNLEQDARMAMRKAATDETLRLPADYSYPHASQAKQE
SQTEAPEIAQIDKMLAARKVQPADKFETPQDFVNTMKPYAEKAAKALGVDSNLLIAQAALETGWGKKVIKNSVDSSHNLF
NIKADRSWQGDKISKNTLEYHDNIAVTENAAFRSYDNYEESFSDFVRFLNQNPRYERALQQTDSSESFIKGIHSAGYATD
PNYSNKVMSVMRKVESLLK

Sequences:

>Translated_339_residues
MQSVKMNNSVNMTDVGFIHDVGGLDKLRKSAVSGDQDSQDKALRAAAEQFESIFTQMLFKSMRNANDSFKSDMMSSDNQQ
FFEQMRDDQMATELSKKGSLGLADMIVAQLGASMNQAAPVQSGNNLEQDARMAMRKAATDETLRLPADYSYPHASQAKQE
SQTEAPEIAQIDKMLAARKVQPADKFETPQDFVNTMKPYAEKAAKALGVDSNLLIAQAALETGWGKKVIKNSVDSSHNLF
NIKADRSWQGDKISKNTLEYHDNIAVTENAAFRSYDNYEESFSDFVRFLNQNPRYERALQQTDSSESFIKGIHSAGYATD
PNYSNKVMSVMRKVESLLK
>Mature_339_residues
MQSVKMNNSVNMTDVGFIHDVGGLDKLRKSAVSGDQDSQDKALRAAAEQFESIFTQMLFKSMRNANDSFKSDMMSSDNQQ
FFEQMRDDQMATELSKKGSLGLADMIVAQLGASMNQAAPVQSGNNLEQDARMAMRKAATDETLRLPADYSYPHASQAKQE
SQTEAPEIAQIDKMLAARKVQPADKFETPQDFVNTMKPYAEKAAKALGVDSNLLIAQAALETGWGKKVIKNSVDSSHNLF
NIKADRSWQGDKISKNTLEYHDNIAVTENAAFRSYDNYEESFSDFVRFLNQNPRYERALQQTDSSESFIKGIHSAGYATD
PNYSNKVMSVMRKVESLLK

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=297, Percent_Identity=31.6498316498317, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 37830; Mature: 37830

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSVKMNNSVNMTDVGFIHDVGGLDKLRKSAVSGDQDSQDKALRAAAEQFESIFTQMLFK
CCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SMRNANDSFKSDMMSSDNQQFFEQMRDDQMATELSKKGSLGLADMIVAQLGASMNQAAPV
HHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCC
QSGNNLEQDARMAMRKAATDETLRLPADYSYPHASQAKQESQTEAPEIAQIDKMLAARKV
CCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHC
QPADKFETPQDFVNTMKPYAEKAAKALGVDSNLLIAQAALETGWGKKVIKNSVDSSHNLF
CCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCEE
NIKADRSWQGDKISKNTLEYHDNIAVTENAAFRSYDNYEESFSDFVRFLNQNPRYERALQ
EEECCCCCCCCCCCHHHHHHCCCEEEECCCHHHCCCCHHHHHHHHHHHHCCCCHHHHHHH
QTDSSESFIKGIHSAGYATDPNYSNKVMSVMRKVESLLK
HCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MQSVKMNNSVNMTDVGFIHDVGGLDKLRKSAVSGDQDSQDKALRAAAEQFESIFTQMLFK
CCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SMRNANDSFKSDMMSSDNQQFFEQMRDDQMATELSKKGSLGLADMIVAQLGASMNQAAPV
HHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCC
QSGNNLEQDARMAMRKAATDETLRLPADYSYPHASQAKQESQTEAPEIAQIDKMLAARKV
CCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHC
QPADKFETPQDFVNTMKPYAEKAAKALGVDSNLLIAQAALETGWGKKVIKNSVDSSHNLF
CCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCEE
NIKADRSWQGDKISKNTLEYHDNIAVTENAAFRSYDNYEESFSDFVRFLNQNPRYERALQ
EEECCCCCCCCCCCHHHHHHCCCEEEECCCHHHCCCCHHHHHHHHHHHHCCCCHHHHHHH
QTDSSESFIKGIHSAGYATDPNYSNKVMSVMRKVESLLK
HCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]