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The map label for this gene is fliA [H]

Identifier: 59712441

GI number: 59712441

Start: 2066504

End: 2067235

Strand: Reverse

Name: fliA [H]

Synonym: VF_1834

Alternate gene names: 59712441

Gene position: 2067235-2066504 (Counterclockwise)

Preceding gene: 59712442

Following gene: 59712440

Centisome position: 71.34

GC content: 40.03

Gene sequence:

>732_bases
ATGTCTGATGGCTTAACTTATGATCATAAAGGCAATGTAAACAGTCAGAAGGCGTTTTTAGAACGTTATTCTGATCTGGT
TAAACGTATTGCTCATCATTTAATGGCGAGATTGCCAGCGAGTGTATTAGTAGATGATCTGATTCAAGCTGGCATGATTG
GTTTATTAGAAGCCCAGCAAAATTATGATGGCAGTAAAGGCGCAAGTTTTGAAACCTATGCTGGGATTCGAATTAGAGGC
TCAATGTTGGATGAAATTCGACGTGGAGATTGGGTTCCTCGCTCAGTACATAAGAACAGTCGGATGATTAATTCGGCAAT
TAGAGAGCTTGAAGCTAAACTTTCTCGGGATCCGACCGATAAAGAGATAGCAGACTATCTAGAATTACCGTTAGATCAAT
ATTATCAGATACTTAATGATGTAAATTGCGGTAAACTAGTTGGTATGGAAGATTTAGGCGTTTCTGATGATGTGATCGCA
ACTGAAGAACAAGCTGATGATAATCTTCCGTTTCAAGGTGTTGCCGATGAACACTTTCGAGTAGCTCTTGTGGAAGCGAT
TAAAACGCTTCCTGAAAGAGAAGCTTTGGTTTTGTCTCTTTATTACGATGAAGAACTTAACCTAAAAGAGATTGGTGCTG
TAATTGGTGTAAGCGAGTCTCGCGTAAGCCAAATACACAGCCAATCGATGCAACGACTACGGGCCAAGTTATCATCTTGG
ACAGTAGAATAA

Upstream 100 bases:

>100_bases
GTGGTAACTTGGCCTGTACCTAAAGCACCAAGCGGTCACTTAGAGTTTTTCGTAGAAAAGCTATTACGCAATGAAAAAAC
AAGTGAAATTGAGGACGATT

Downstream 100 bases:

>100_bases
TTAAATCCCGTTACTTTGCCCTCACTGGAGGCGATTTTGAATAAAAACATGAAGATCCTTATTGTTGATGACTTTTCAAC
AATGCGCCGTATTGTAAAAA

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSDGLTYDHKGNVNSQKAFLERYSDLVKRIAHHLMARLPASVLVDDLIQAGMIGLLEAQQNYDGSKGASFETYAGIRIRG
SMLDEIRRGDWVPRSVHKNSRMINSAIRELEAKLSRDPTDKEIADYLELPLDQYYQILNDVNCGKLVGMEDLGVSDDVIA
TEEQADDNLPFQGVADEHFRVALVEAIKTLPEREALVLSLYYDEELNLKEIGAVIGVSESRVSQIHSQSMQRLRAKLSSW
TVE

Sequences:

>Translated_243_residues
MSDGLTYDHKGNVNSQKAFLERYSDLVKRIAHHLMARLPASVLVDDLIQAGMIGLLEAQQNYDGSKGASFETYAGIRIRG
SMLDEIRRGDWVPRSVHKNSRMINSAIRELEAKLSRDPTDKEIADYLELPLDQYYQILNDVNCGKLVGMEDLGVSDDVIA
TEEQADDNLPFQGVADEHFRVALVEAIKTLPEREALVLSLYYDEELNLKEIGAVIGVSESRVSQIHSQSMQRLRAKLSSW
TVE
>Mature_242_residues
SDGLTYDHKGNVNSQKAFLERYSDLVKRIAHHLMARLPASVLVDDLIQAGMIGLLEAQQNYDGSKGASFETYAGIRIRGS
MLDEIRRGDWVPRSVHKNSRMINSAIRELEAKLSRDPTDKEIADYLELPLDQYYQILNDVNCGKLVGMEDLGVSDDVIAT
EEQADDNLPFQGVADEHFRVALVEAIKTLPEREALVLSLYYDEELNLKEIGAVIGVSESRVSQIHSQSMQRLRAKLSSWT
VE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=229, Percent_Identity=49.7816593886463, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1789448, Length=200, Percent_Identity=27, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789098, Length=241, Percent_Identity=27.8008298755187, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27309; Mature: 27178

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00018 EF_HAND_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDGLTYDHKGNVNSQKAFLERYSDLVKRIAHHLMARLPASVLVDDLIQAGMIGLLEAQQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
NYDGSKGASFETYAGIRIRGSMLDEIRRGDWVPRSVHKNSRMINSAIRELEAKLSRDPTD
CCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
KEIADYLELPLDQYYQILNDVNCGKLVGMEDLGVSDDVIATEEQADDNLPFQGVADEHFR
HHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
VALVEAIKTLPEREALVLSLYYDEELNLKEIGAVIGVSESRVSQIHSQSMQRLRAKLSSW
HHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
TVE
CCC
>Mature Secondary Structure 
SDGLTYDHKGNVNSQKAFLERYSDLVKRIAHHLMARLPASVLVDDLIQAGMIGLLEAQQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
NYDGSKGASFETYAGIRIRGSMLDEIRRGDWVPRSVHKNSRMINSAIRELEAKLSRDPTD
CCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
KEIADYLELPLDQYYQILNDVNCGKLVGMEDLGVSDDVIATEEQADDNLPFQGVADEHFR
HHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
VALVEAIKTLPEREALVLSLYYDEELNLKEIGAVIGVSESRVSQIHSQSMQRLRAKLSSW
HHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
TVE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]