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The map label for this gene is rluE [H]

Identifier: 59712383

GI number: 59712383

Start: 2005084

End: 2005704

Strand: Direct

Name: rluE [H]

Synonym: VF_1776

Alternate gene names: 59712383

Gene position: 2005084-2005704 (Clockwise)

Preceding gene: 59712381

Following gene: 172087727

Centisome position: 69.2

GC content: 40.42

Gene sequence:

>621_bases
ATGACATTCAAACGCTCCCCTAATTGGAAAAAACCGGCTTTTAAGAAAAAAAACACACAAAAGCCACGCCCTGCACCTAA
AGATCGTAAAGTTATTATCTTTAATAAACCTTTTGATGTATTGAGTCAATTTACTGATGACCAACATCGCCAGACCCTCG
CAGATTTTATTTCTGTTAAAGACGTTTATGCTGCAGGCCGTCTTGATCGCGATAGCGAAGGTTTATTAATTTTAACTAAT
GATGGAGTGTTACAAGCTCGATTAACTCAACCAGAATCTAAATCACCAAAAACGTATTGGGTACAAGTTGAAGGCGCACC
TACTGAAGATGATTTAGATAAATTACGTCAAGGGGTTGAGCTAAAAGATGGCATGACGTTGCCAGCAAAAGTAGAAATCA
TACCGGAACCAAACGTGTGGGAACGCAATCCACCTGTTCGTTTTCGTGCCGCTATTCCAACAACATGGATTTCAATTACG
ATCACTGAAGGTCGTAACCGACAAGTTCGCCGTATGACAGCAAACATTGGTTTCCCTACCTTACGCTTAATACGCTATTC
AATGGGAGATTGGACAATAAAAGATGTGGCTCACGGCACTTGGAAAGAAGTATCACTTTAA

Upstream 100 bases:

>100_bases
TTTTTATTAGTCTCTTGTTTCACGCGCACACTATAGCGAAAAGCGGTGAACTTTAAAACTAAACACCACAAAAGCTTTAC
AAATCTGCAAGGTAGTTAAC

Downstream 100 bases:

>100_bases
AAACAATAAATTAAATTGTAATAAAAACGTTAATTTTACTATCTGTTTACTGCTTTTTAAATTAGACTGACTACATTAAT
TATAAAAAATAAAGGGATCA

Product: 23S rRNA pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTFKRSPNWKKPAFKKKNTQKPRPAPKDRKVIIFNKPFDVLSQFTDDQHRQTLADFISVKDVYAAGRLDRDSEGLLILTN
DGVLQARLTQPESKSPKTYWVQVEGAPTEDDLDKLRQGVELKDGMTLPAKVEIIPEPNVWERNPPVRFRAAIPTTWISIT
ITEGRNRQVRRMTANIGFPTLRLIRYSMGDWTIKDVAHGTWKEVSL

Sequences:

>Translated_206_residues
MTFKRSPNWKKPAFKKKNTQKPRPAPKDRKVIIFNKPFDVLSQFTDDQHRQTLADFISVKDVYAAGRLDRDSEGLLILTN
DGVLQARLTQPESKSPKTYWVQVEGAPTEDDLDKLRQGVELKDGMTLPAKVEIIPEPNVWERNPPVRFRAAIPTTWISIT
ITEGRNRQVRRMTANIGFPTLRLIRYSMGDWTIKDVAHGTWKEVSL
>Mature_205_residues
TFKRSPNWKKPAFKKKNTQKPRPAPKDRKVIIFNKPFDVLSQFTDDQHRQTLADFISVKDVYAAGRLDRDSEGLLILTND
GVLQARLTQPESKSPKTYWVQVEGAPTEDDLDKLRQGVELKDGMTLPAKVEIIPEPNVWERNPPVRFRAAIPTTWISITI
TEGRNRQVRRMTANIGFPTLRLIRYSMGDWTIKDVAHGTWKEVSL

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=193, Percent_Identity=60.1036269430052, Blast_Score=249, Evalue=9e-68,
Organism=Escherichia coli, GI1787524, Length=182, Percent_Identity=33.5164835164835, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788510, Length=179, Percent_Identity=31.2849162011173, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790453, Length=186, Percent_Identity=29.0322580645161, Blast_Score=77, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 23683; Mature: 23552

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFKRSPNWKKPAFKKKNTQKPRPAPKDRKVIIFNKPFDVLSQFTDDQHRQTLADFISVK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHCCHHHHHHHHHHHHHH
DVYAAGRLDRDSEGLLILTNDGVLQARLTQPESKSPKTYWVQVEGAPTEDDLDKLRQGVE
HHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHCCCC
LKDGMTLPAKVEIIPEPNVWERNPPVRFRAAIPTTWISITITEGRNRQVRRMTANIGFPT
CCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCEEEEEEEEECCCCCEEEEEEECCCCCC
LRLIRYSMGDWTIKDVAHGTWKEVSL
EEHEEHHCCCEEEHHHCCCCEEECCC
>Mature Secondary Structure 
TFKRSPNWKKPAFKKKNTQKPRPAPKDRKVIIFNKPFDVLSQFTDDQHRQTLADFISVK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHCCHHHHHHHHHHHHHH
DVYAAGRLDRDSEGLLILTNDGVLQARLTQPESKSPKTYWVQVEGAPTEDDLDKLRQGVE
HHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHCCCC
LKDGMTLPAKVEIIPEPNVWERNPPVRFRAAIPTTWISITITEGRNRQVRRMTANIGFPT
CCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCEEEEEEEEECCCCCEEEEEEECCCCCC
LRLIRYSMGDWTIKDVAHGTWKEVSL
EEHEEHHCCCEEEHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA