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The map label for this gene is folD [H]

Identifier: 59712377

GI number: 59712377

Start: 1996663

End: 1997520

Strand: Direct

Name: folD [H]

Synonym: VF_1770

Alternate gene names: 59712377

Gene position: 1996663-1997520 (Clockwise)

Preceding gene: 59712375

Following gene: 172087726

Centisome position: 68.91

GC content: 42.42

Gene sequence:

>858_bases
ATGACCGCTCAAATTATCGATGGAAAACTCATTTCTCAAACCGTTCGCTCTGAAGTCGGGGCTCGTGTTAAAGCTCGTGT
AGAAGCAGGATTACGTGCTCCAGGCCTTGCAGTTGTATTAGTTGGACAAGACCCTGCATCTCAAGTTTATGTAGGTAGTA
AACGTCGTGCCTGTGAAGAAGTCGGTTTCGTTTCAAAGTCATATGACCTTCCAACGACCACATCTGAATCAGAACTTCTT
AACCTAATTGATGAGTTAAACCAAGATCCTGAAATTGATGGTATTTTGGTTCAATTGCCTCTTCCAGCAGGAATGGATAG
CACTAAAATTTTAGAGCATATCGATCCTGAAAAAGACGTTGATGGTTTCCACCCATATAACGTAGGTCGATTATCACAAC
GTATTCCAAAACTGCGTTCTTGTACACCAAAAGGGATCATCACTCTACTTGACCGCTACAACATTCAAGTTCGTGGCATG
CATGCCGTTGTTGTTGGTGCTTCAAATATTGTTGGTCGACCAATGACACTTGAATTACTTCTTGCTGGTTGTACTACCAC
CACTTGTCATCGCTTTACAAAAGATTTGGAAAGCCATATTCGTCAAGCTGATTTGGTTGTGGTCGCCGTCGGTAAACCAA
ATTTCATTCCAGGTGAATGGATTAAAGAAGGTGCTGTCGTTGTTGATGTAGGTATTAATCGACTGGATTCAGGTAAGTTA
ATTGGCGATGTCGAGTATGACGTAGCCAAAACAAAAGCAAGCTATATTACTCCAGTACCAGGTGGAGTTGGTCCAATGAC
AGTAGCGACGCTTATCGAAAACACCCTACTTGCCTGTGAGCAATACCACAGCAAATAA

Upstream 100 bases:

>100_bases
TAAAAAACCCCCGAACGCAAACGTTTGCTCTATAGCTATTCTGAATTAATTCTGCGACAATACTTAGGTCAACCAGTAAC
TGCCCTTTAGAAGGATCACT

Downstream 100 bases:

>100_bases
CTTTTAATAGTTACAAAACTATCAATTAGCGAGCAATTTTACTGCTCGCTTTTTTATATCTGCGCTTCCCCACACTTCTT
GACCAAATTTCACTCTATTT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTAQIIDGKLISQTVRSEVGARVKARVEAGLRAPGLAVVLVGQDPASQVYVGSKRRACEEVGFVSKSYDLPTTTSESELL
NLIDELNQDPEIDGILVQLPLPAGMDSTKILEHIDPEKDVDGFHPYNVGRLSQRIPKLRSCTPKGIITLLDRYNIQVRGM
HAVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKDLESHIRQADLVVVAVGKPNFIPGEWIKEGAVVVDVGINRLDSGKL
IGDVEYDVAKTKASYITPVPGGVGPMTVATLIENTLLACEQYHSK

Sequences:

>Translated_285_residues
MTAQIIDGKLISQTVRSEVGARVKARVEAGLRAPGLAVVLVGQDPASQVYVGSKRRACEEVGFVSKSYDLPTTTSESELL
NLIDELNQDPEIDGILVQLPLPAGMDSTKILEHIDPEKDVDGFHPYNVGRLSQRIPKLRSCTPKGIITLLDRYNIQVRGM
HAVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKDLESHIRQADLVVVAVGKPNFIPGEWIKEGAVVVDVGINRLDSGKL
IGDVEYDVAKTKASYITPVPGGVGPMTVATLIENTLLACEQYHSK
>Mature_284_residues
TAQIIDGKLISQTVRSEVGARVKARVEAGLRAPGLAVVLVGQDPASQVYVGSKRRACEEVGFVSKSYDLPTTTSESELLN
LIDELNQDPEIDGILVQLPLPAGMDSTKILEHIDPEKDVDGFHPYNVGRLSQRIPKLRSCTPKGIITLLDRYNIQVRGMH
AVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKDLESHIRQADLVVVAVGKPNFIPGEWIKEGAVVVDVGINRLDSGKLI
GDVEYDVAKTKASYITPVPGGVGPMTVATLIENTLLACEQYHSK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=298, Percent_Identity=41.6107382550336, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=43.1972789115646, Blast_Score=222, Evalue=3e-58,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=43.8775510204082, Blast_Score=215, Evalue=4e-56,
Organism=Homo sapiens, GI36796743, Length=209, Percent_Identity=30.1435406698565, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1786741, Length=283, Percent_Identity=73.8515901060071, Blast_Score=446, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17568735, Length=289, Percent_Identity=41.1764705882353, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=43.6241610738255, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=42.5087108013937, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6322933, Length=312, Percent_Identity=26.6025641025641, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=46.5986394557823, Blast_Score=250, Evalue=9e-67,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=46.5986394557823, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI62472483, Length=290, Percent_Identity=41.3793103448276, Blast_Score=213, Evalue=1e-55,
Organism=Drosophila melanogaster, GI45551871, Length=290, Percent_Identity=41.3793103448276, Blast_Score=213, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24645718, Length=290, Percent_Identity=41.3793103448276, Blast_Score=213, Evalue=1e-55,
Organism=Drosophila melanogaster, GI17137370, Length=290, Percent_Identity=41.3793103448276, Blast_Score=213, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30790; Mature: 30659

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQIIDGKLISQTVRSEVGARVKARVEAGLRAPGLAVVLVGQDPASQVYVGSKRRACEE
CCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEECCCCCCCEEECCHHHHHHH
VGFVSKSYDLPTTTSESELLNLIDELNQDPEIDGILVQLPLPAGMDSTKILEHIDPEKDV
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCC
DGFHPYNVGRLSQRIPKLRSCTPKGIITLLDRYNIQVRGMHAVVVGASNIVGRPMTLELL
CCCCCCCHHHHHHHCHHHHCCCCHHHHHHHHHCCEEEEEEEEEEEECHHHCCCCCEEEHH
LAGCTTTTCHRFTKDLESHIRQADLVVVAVGKPNFIPGEWIKEGAVVVDVGINRLDSGKL
HHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHCCCEEEEECCCCCCCCCE
IGDVEYDVAKTKASYITPVPGGVGPMTVATLIENTLLACEQYHSK
EECCCHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAQIIDGKLISQTVRSEVGARVKARVEAGLRAPGLAVVLVGQDPASQVYVGSKRRACEE
CCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEECCCCCCCEEECCHHHHHHH
VGFVSKSYDLPTTTSESELLNLIDELNQDPEIDGILVQLPLPAGMDSTKILEHIDPEKDV
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCC
DGFHPYNVGRLSQRIPKLRSCTPKGIITLLDRYNIQVRGMHAVVVGASNIVGRPMTLELL
CCCCCCCHHHHHHHCHHHHCCCCHHHHHHHHHCCEEEEEEEEEEEECHHHCCCCCEEEHH
LAGCTTTTCHRFTKDLESHIRQADLVVVAVGKPNFIPGEWIKEGAVVVDVGINRLDSGKL
HHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHCCCEEEEECCCCCCCCCE
IGDVEYDVAKTKASYITPVPGGVGPMTVATLIENTLLACEQYHSK
EECCCHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA