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The map label for this gene is cspD [H]

Identifier: 59712374

GI number: 59712374

Start: 1993824

End: 1994045

Strand: Direct

Name: cspD [H]

Synonym: VF_1767

Alternate gene names: 59712374

Gene position: 1993824-1994045 (Clockwise)

Preceding gene: 59712371

Following gene: 59712375

Centisome position: 68.81

GC content: 43.24

Gene sequence:

>222_bases
ATGGCAACAGGTACAGTAAAGTGGTTCAATAACGCCAAAGGGTTTGGCTTCATTTGTCCTGAAGGGGAGGATGGCGATAT
TTTTGCTCATTACTCCACCATTGAAATGGAGGGATATCGAACTCTAAAAGCAGGACAAGCCGTCACTTACGAAATCCACG
AAGGCCCAAAAGGTTTTCACGCAAGTGAAATTAAATTACTCGAAGGTGAAAACGCAAAGTAG

Upstream 100 bases:

>100_bases
TATTTAATAGAGTGTACTTGTAGTGGTTATCAATTGAACAGATAAATGATTAATTCATAACCATTGTGCTAGGTAATTAA
AATGCAGAGGGATGTATAGC

Downstream 100 bases:

>100_bases
CTGCTTTATTTTCAAGTTTATAAAAAAGGTGAGTCAACATGCTCACCTTTTTTGAACCAATCGTTTCAAAACCACTCCAA
TCTTAATTCACTTCTGTTAG

Product: DNA replication inhibitor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 73; Mature: 72

Protein sequence:

>73_residues
MATGTVKWFNNAKGFGFICPEGEDGDIFAHYSTIEMEGYRTLKAGQAVTYEIHEGPKGFHASEIKLLEGENAK

Sequences:

>Translated_73_residues
MATGTVKWFNNAKGFGFICPEGEDGDIFAHYSTIEMEGYRTLKAGQAVTYEIHEGPKGFHASEIKLLEGENAK
>Mature_72_residues
ATGTVKWFNNAKGFGFICPEGEDGDIFAHYSTIEMEGYRTLKAGQAVTYEIHEGPKGFHASEIKLLEGENAK

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=72, Percent_Identity=72.2222222222222, Blast_Score=111, Evalue=1e-26,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=50, Blast_Score=71, Evalue=1e-14,
Organism=Escherichia coli, GI1787839, Length=66, Percent_Identity=48.4848484848485, Blast_Score=70, Evalue=3e-14,
Organism=Escherichia coli, GI1789979, Length=65, Percent_Identity=47.6923076923077, Blast_Score=70, Evalue=4e-14,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=46.875, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI2367114, Length=65, Percent_Identity=43.0769230769231, Blast_Score=64, Evalue=1e-12,
Organism=Escherichia coli, GI1787834, Length=65, Percent_Identity=46.1538461538462, Blast_Score=64, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012751
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 8040; Mature: 7909

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGTVKWFNNAKGFGFICPEGEDGDIFAHYSTIEMEGYRTLKAGQAVTYEIHEGPKGFH
CCCCEEEECCCCCCCEEECCCCCCCCEEEEEEEEEECCCEEECCCCEEEEEECCCCCCCC
ASEIKLLEGENAK
CCEEEEEECCCCC
>Mature Secondary Structure 
ATGTVKWFNNAKGFGFICPEGEDGDIFAHYSTIEMEGYRTLKAGQAVTYEIHEGPKGFH
CCCEEEECCCCCCCEEECCCCCCCCEEEEEEEEEECCCEEECCCCEEEEEECCCCCCCC
ASEIKLLEGENAK
CCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]