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The map label for this gene is holB [H]

Identifier: 59712341

GI number: 59712341

Start: 1958346

End: 1959152

Strand: Reverse

Name: holB [H]

Synonym: VF_1734

Alternate gene names: 59712341

Gene position: 1959152-1958346 (Counterclockwise)

Preceding gene: 59712342

Following gene: 59712340

Centisome position: 67.61

GC content: 36.43

Gene sequence:

>807_bases
GTGGTTTTTGTCATAGTGTGCGAGTTAATTAAATCACGAACGCATCCTGATCTGCATTGGATAAAACCAGAAACTGAAGG
TAAATCTATTTCAGTGGAACAAATACGCCAATGTAATTCATGGGCTTTAGAGTCCTCACAATTCAACGCTAAGCGTGTGA
TCATCATTGATCCTGCAGAAAAAATGACAGAATCTGCAGCTAATGCATTACTTAAGACATTAGAGGCACCACCGAAAAAT
TGTCAATTTGTATTGTTGGCCGATTCACCTCATCAGTTATTACCAACAATACGTAGTCGTTGTCAGGTATGGCATCAATC
GAAAATTGATGCTGAATCTATTACAATCTGGCTTAAAGAAACTGAAAACCTTTCAGTATCAATGCAGTCTATTGCTTTGA
ATGATTCACTCCCATTAGCAGTGAAAAGCTTTTATCAAGATAAGAAACAAGCATTACATAAACAGCTACTTTCAGACTTT
GAATCCTGTGTGAAAAGTGATTTTTGCCAGACTGCTGATGTAGTTAAAACTGTTCTAAAACTTGAAAGTGAAGGCTTAAC
TTGGCTAAGTTACTTACTTGCTGATATTCAGAAGAGACAGATGGGAGTAATGCAGCCTTGGGTTCATTGTGACGAAATAA
AGCTCATAGAAAGCTTAGCGAAAATATTATCGAGTAATGTGGTCTATAAGCAATACATTGCACTGAATTCGTTACAAGAG
CAATTGGATAGTTTCCCTGGTTTAAATAAAGAATTACTACTAACTCAATGGATGTTTGAGTTTACTGGAGAGTTGAGTGT
TAATTGA

Upstream 100 bases:

>100_bases
ATGCCATATTGTGTGCTATGCCTGTAGGGGCAGGACGAGAAGCGTTAGTTCAGTATTTTGCTGATACGCTATTGTGTTTA
ACGCCAAGGTATTGAACCTT

Downstream 100 bases:

>100_bases
TTCACACTGTCATTTAGATAAATTAAATTATGATGACTTACATGTAAATGTTGCTGATGTACTTGATAAAGCGAAGAAAC
AAGGAGTTGAGCAGTTTTTA

Product: DNA polymerase III, delta prime subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MVFVIVCELIKSRTHPDLHWIKPETEGKSISVEQIRQCNSWALESSQFNAKRVIIIDPAEKMTESAANALLKTLEAPPKN
CQFVLLADSPHQLLPTIRSRCQVWHQSKIDAESITIWLKETENLSVSMQSIALNDSLPLAVKSFYQDKKQALHKQLLSDF
ESCVKSDFCQTADVVKTVLKLESEGLTWLSYLLADIQKRQMGVMQPWVHCDEIKLIESLAKILSSNVVYKQYIALNSLQE
QLDSFPGLNKELLLTQWMFEFTGELSVN

Sequences:

>Translated_268_residues
MVFVIVCELIKSRTHPDLHWIKPETEGKSISVEQIRQCNSWALESSQFNAKRVIIIDPAEKMTESAANALLKTLEAPPKN
CQFVLLADSPHQLLPTIRSRCQVWHQSKIDAESITIWLKETENLSVSMQSIALNDSLPLAVKSFYQDKKQALHKQLLSDF
ESCVKSDFCQTADVVKTVLKLESEGLTWLSYLLADIQKRQMGVMQPWVHCDEIKLIESLAKILSSNVVYKQYIALNSLQE
QLDSFPGLNKELLLTQWMFEFTGELSVN
>Mature_268_residues
MVFVIVCELIKSRTHPDLHWIKPETEGKSISVEQIRQCNSWALESSQFNAKRVIIIDPAEKMTESAANALLKTLEAPPKN
CQFVLLADSPHQLLPTIRSRCQVWHQSKIDAESITIWLKETENLSVSMQSIALNDSLPLAVKSFYQDKKQALHKQLLSDF
ESCVKSDFCQTADVVKTVLKLESEGLTWLSYLLADIQKRQMGVMQPWVHCDEIKLIESLAKILSSNVVYKQYIALNSLQE
QLDSFPGLNKELLLTQWMFEFTGELSVN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=261, Percent_Identity=28.735632183908, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 30539; Mature: 30539

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFVIVCELIKSRTHPDLHWIKPETEGKSISVEQIRQCNSWALESSQFNAKRVIIIDPAE
CHHHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHCCHHHHCCCCCCCCEEEEEECCHH
KMTESAANALLKTLEAPPKNCQFVLLADSPHQLLPTIRSRCQVWHQSKIDAESITIWLKE
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
TENLSVSMQSIALNDSLPLAVKSFYQDKKQALHKQLLSDFESCVKSDFCQTADVVKTVLK
CCCCEEEHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LESEGLTWLSYLLADIQKRQMGVMQPWVHCDEIKLIESLAKILSSNVVYKQYIALNSLQE
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
QLDSFPGLNKELLLTQWMFEFTGELSVN
HHHHCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVFVIVCELIKSRTHPDLHWIKPETEGKSISVEQIRQCNSWALESSQFNAKRVIIIDPAE
CHHHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHCCHHHHCCCCCCCCEEEEEECCHH
KMTESAANALLKTLEAPPKNCQFVLLADSPHQLLPTIRSRCQVWHQSKIDAESITIWLKE
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
TENLSVSMQSIALNDSLPLAVKSFYQDKKQALHKQLLSDFESCVKSDFCQTADVVKTVLK
CCCCEEEHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LESEGLTWLSYLLADIQKRQMGVMQPWVHCDEIKLIESLAKILSSNVVYKQYIALNSLQE
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
QLDSFPGLNKELLLTQWMFEFTGELSVN
HHHHCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430; 10491164 [H]