Click here to switch to the map view.

The map label for this gene is metR [H]

Identifier: 59712329

GI number: 59712329

Start: 1941551

End: 1942462

Strand: Direct

Name: metR [H]

Synonym: VF_1722

Alternate gene names: 59712329

Gene position: 1941551-1942462 (Clockwise)

Preceding gene: 172087722

Following gene: 59712331

Centisome position: 67.01

GC content: 39.58

Gene sequence:

>912_bases
ATGATTGAATTAAAACATCTAAAAACCTTAACTACGCTGCGTGATACGGGATCGTTAACCGCAACAGCAAACGCACTTCA
TCTCACTCAATCGGCGTTATCGCATCAACTTAAAGATTTTGAAACACGTTTAGGCAGCAGCGTATTTTTACGAAAAACAC
GCCCAGTAAAGTTCACCTCTGAAGGTGAGATCCTATTAAAACTCGCCGATGATCTTTTACCTAAGTTAGCAAAAGCGGAA
TACGAAATTGCCAGTTTGAAAGAAGATCTACATGGAAGATTACACATGGCGATTGAATGCCACTCCTGCTTTCAATGGCT
AATGCCAGCAATTAAAGAATATCAAATCAGTTGGCCTGAAGTGGAATTAGATTTCTCATCGGGTTTTGGGTTTGAGCCTT
TACCTGCTTTGGTTAATGGTGATTTAGATCTTGTTATTACCTCTGATATTCAGCCACGCAGTGAAGTGCATTATGAACCT
CTCTTTGATTTTGAAATGCGCTTAATCATGGCAACCAATCACCCACTTGCTAATAAAGACAGCATTGAACCAAAAGACTT
ATTAGATCAAACCATGTTGACTTATCCGGTACAAAAGCAACGATTGGATGTCGTTAAGCATTTCTTATCACCGAATCATG
TAGAGCCAGCAAAATGGAAACAAGCCGAAAACACATTGATGTTAGTACAAATGGTATCAGCAGGTTTAGGAGTAGCAGCT
CTACCAAATTGGGCTATCAGTGAGTTTTCACGACAAGGACTAATTGAGAGTAAACCGTTAGGGAAAGGATTGTGGCGAAG
ATTGTTCGCTGCCGTTAGAGACAGCGAACATGATAAAAAATATTTACAAGCATTTTTCTCTACTGCGAAACAGCAGAGCC
AGAGTCATTTAGTTGGGATTAAAATGACTTAA

Upstream 100 bases:

>100_bases
TCGATTAACTTCATTTAGCCGTCTAGATGTTTACTTTGTTGTCTTTTCACTTTACTGTGAAGATAATTAAACTTACTCAA
ACGGAACTTGAGGATCAGTA

Downstream 100 bases:

>100_bases
ATTGATTTGTTAATGGGTCATATTGGTAACCCAAACTACTGAGCTTGTCTTGAACTCCTTGTGTATCAAGCTCATACATG
CTGGATAACTCTTCTAGACT

Product: DNA-binding transcriptional activator, homocysteine-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MIELKHLKTLTTLRDTGSLTATANALHLTQSALSHQLKDFETRLGSSVFLRKTRPVKFTSEGEILLKLADDLLPKLAKAE
YEIASLKEDLHGRLHMAIECHSCFQWLMPAIKEYQISWPEVELDFSSGFGFEPLPALVNGDLDLVITSDIQPRSEVHYEP
LFDFEMRLIMATNHPLANKDSIEPKDLLDQTMLTYPVQKQRLDVVKHFLSPNHVEPAKWKQAENTLMLVQMVSAGLGVAA
LPNWAISEFSRQGLIESKPLGKGLWRRLFAAVRDSEHDKKYLQAFFSTAKQQSQSHLVGIKMT

Sequences:

>Translated_303_residues
MIELKHLKTLTTLRDTGSLTATANALHLTQSALSHQLKDFETRLGSSVFLRKTRPVKFTSEGEILLKLADDLLPKLAKAE
YEIASLKEDLHGRLHMAIECHSCFQWLMPAIKEYQISWPEVELDFSSGFGFEPLPALVNGDLDLVITSDIQPRSEVHYEP
LFDFEMRLIMATNHPLANKDSIEPKDLLDQTMLTYPVQKQRLDVVKHFLSPNHVEPAKWKQAENTLMLVQMVSAGLGVAA
LPNWAISEFSRQGLIESKPLGKGLWRRLFAAVRDSEHDKKYLQAFFSTAKQQSQSHLVGIKMT
>Mature_303_residues
MIELKHLKTLTTLRDTGSLTATANALHLTQSALSHQLKDFETRLGSSVFLRKTRPVKFTSEGEILLKLADDLLPKLAKAE
YEIASLKEDLHGRLHMAIECHSCFQWLMPAIKEYQISWPEVELDFSSGFGFEPLPALVNGDLDLVITSDIQPRSEVHYEP
LFDFEMRLIMATNHPLANKDSIEPKDLLDQTMLTYPVQKQRLDVVKHFLSPNHVEPAKWKQAENTLMLVQMVSAGLGVAA
LPNWAISEFSRQGLIESKPLGKGLWRRLFAAVRDSEHDKKYLQAFFSTAKQQSQSHLVGIKMT

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=301, Percent_Identity=53.4883720930233, Blast_Score=345, Evalue=3e-96,
Organism=Escherichia coli, GI1786401, Length=265, Percent_Identity=27.5471698113208, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI145693105, Length=276, Percent_Identity=24.2753623188406, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788887, Length=245, Percent_Identity=24.0816326530612, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790399, Length=258, Percent_Identity=27.906976744186, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790208, Length=253, Percent_Identity=26.4822134387352, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI157672245, Length=228, Percent_Identity=21.9298245614035, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788297, Length=247, Percent_Identity=24.6963562753036, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787879, Length=265, Percent_Identity=23.3962264150943, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787601, Length=191, Percent_Identity=25.130890052356, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788748, Length=243, Percent_Identity=22.2222222222222, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34318; Mature: 34318

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50931 HTH_LYSR ; PS00300 SRP54

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELKHLKTLTTLRDTGSLTATANALHLTQSALSHQLKDFETRLGSSVFLRKTRPVKFTS
CCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECC
EGEILLKLADDLLPKLAKAEYEIASLKEDLHGRLHMAIECHSCFQWLMPAIKEYQISWPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCCE
VELDFSSGFGFEPLPALVNGDLDLVITSDIQPRSEVHYEPLFDFEMRLIMATNHPLANKD
EEEECCCCCCCCCCHHHHCCCEEEEEECCCCCCHHCCCCCCCCCEEEEEEEECCCCCCCC
SIEPKDLLDQTMLTYPVQKQRLDVVKHFLSPNHVEPAKWKQAENTLMLVQMVSAGLGVAA
CCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCHH
LPNWAISEFSRQGLIESKPLGKGLWRRLFAAVRDSEHDKKYLQAFFSTAKQQSQSHLVGI
CCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHCEEEE
KMT
EEC
>Mature Secondary Structure
MIELKHLKTLTTLRDTGSLTATANALHLTQSALSHQLKDFETRLGSSVFLRKTRPVKFTS
CCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECC
EGEILLKLADDLLPKLAKAEYEIASLKEDLHGRLHMAIECHSCFQWLMPAIKEYQISWPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCCE
VELDFSSGFGFEPLPALVNGDLDLVITSDIQPRSEVHYEPLFDFEMRLIMATNHPLANKD
EEEECCCCCCCCCCHHHHCCCEEEEEECCCCCCHHCCCCCCCCCEEEEEEEECCCCCCCC
SIEPKDLLDQTMLTYPVQKQRLDVVKHFLSPNHVEPAKWKQAENTLMLVQMVSAGLGVAA
CCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCHH
LPNWAISEFSRQGLIESKPLGKGLWRRLFAAVRDSEHDKKYLQAFFSTAKQQSQSHLVGI
CCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHCEEEE
KMT
EEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]