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The map label for this gene is purU [H]

Identifier: 59712321

GI number: 59712321

Start: 1930975

End: 1931670

Strand: Direct

Name: purU [H]

Synonym: VF_1714

Alternate gene names: 59712321

Gene position: 1930975-1931670 (Clockwise)

Preceding gene: 59712313

Following gene: 59712324

Centisome position: 66.64

GC content: 38.79

Gene sequence:

>696_bases
ATGAGAACAGAGCTAGAGGGCATTTTTAATGATGTCACTTTCCTAGCAGATATTGATGAAGCACTCCCATCAGGAAGTCA
TCGTAAATTGGTGACTGAGCCACGAAAAAAAGTGGTTATTCTCGTCACTAAAGAAGCACACTGTATTGGTGATATTCTAA
TTAAAGCCTATTCTGGTGCGATGAATATCGATATTGCTGCAGTTGTGGGAAATCACGATGTATTAGGCGGCCTCATTGAA
AAGTTCGATATCCCTTTTCACTACGTTTCCCATGAAGGGCTATCACGCGAAGAGCATGAAGAGAAAATGCTTGAAGTGAT
TAACTCTTATGAACCAGAATACGTTGTATTAGCAAAATATATGCGTGTATTAACACCAAACTTTGTTGCTCAATTCCCTA
AAAAAATCATTAACATCCACCACAGTTTCTTACCTGCTTTCATTGGAGCAAAACCTTATCAGCAAGCTTATGACCGCGGT
GTAAAAATCATTGGTGCCACCGCACACTTTGTAACGAATGATTTAGATGAAGGTCCAATCATTAAACAAGATGTTATCCC
TGTGGATCACAACTTTAGTGCTGAAGATATGGCAATGGCTGGTCGTGACGTAGAGAAATCAGTATTGAGTAAAGCGCTAA
CAAAAGTATTGAACGATCACGTTTTTGTTTACGGCAATAAAACCGTTATTCTTTAA

Upstream 100 bases:

>100_bases
CGTGGGCTAATAGCAAAAATAACCAACATTTGTTACAAACATCAGCTAAACATTGCTTCATAACAATGAATACGTTGATA
ACGTAACAGGTCAGTTTTTT

Downstream 100 bases:

>100_bases
AGACTGAAATAAGAAACGGCGATCATTATGATCGCCGTTTTTATTTTTAATTCATTGTTATTGAGCCGCAATAAACTCTA
TTGCGTATTTATTAAATTCT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MRTELEGIFNDVTFLADIDEALPSGSHRKLVTEPRKKVVILVTKEAHCIGDILIKAYSGAMNIDIAAVVGNHDVLGGLIE
KFDIPFHYVSHEGLSREEHEEKMLEVINSYEPEYVVLAKYMRVLTPNFVAQFPKKIINIHHSFLPAFIGAKPYQQAYDRG
VKIIGATAHFVTNDLDEGPIIKQDVIPVDHNFSAEDMAMAGRDVEKSVLSKALTKVLNDHVFVYGNKTVIL

Sequences:

>Translated_231_residues
MRTELEGIFNDVTFLADIDEALPSGSHRKLVTEPRKKVVILVTKEAHCIGDILIKAYSGAMNIDIAAVVGNHDVLGGLIE
KFDIPFHYVSHEGLSREEHEEKMLEVINSYEPEYVVLAKYMRVLTPNFVAQFPKKIINIHHSFLPAFIGAKPYQQAYDRG
VKIIGATAHFVTNDLDEGPIIKQDVIPVDHNFSAEDMAMAGRDVEKSVLSKALTKVLNDHVFVYGNKTVIL
>Mature_231_residues
MRTELEGIFNDVTFLADIDEALPSGSHRKLVTEPRKKVVILVTKEAHCIGDILIKAYSGAMNIDIAAVVGNHDVLGGLIE
KFDIPFHYVSHEGLSREEHEEKMLEVINSYEPEYVVLAKYMRVLTPNFVAQFPKKIINIHHSFLPAFIGAKPYQQAYDRG
VKIIGATAHFVTNDLDEGPIIKQDVIPVDHNFSAEDMAMAGRDVEKSVLSKALTKVLNDHVFVYGNKTVIL

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=175, Percent_Identity=30.8571428571429, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI209869995, Length=175, Percent_Identity=30.8571428571429, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI209869993, Length=175, Percent_Identity=30.8571428571429, Blast_Score=88, Evalue=7e-18,
Organism=Escherichia coli, GI1787483, Length=231, Percent_Identity=70.5627705627706, Blast_Score=349, Evalue=7e-98,
Organism=Escherichia coli, GI1788846, Length=103, Percent_Identity=39.8058252427184, Blast_Score=88, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17567511, Length=167, Percent_Identity=37.125748502994, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24582400, Length=196, Percent_Identity=27.0408163265306, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 25811; Mature: 25811

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTELEGIFNDVTFLADIDEALPSGSHRKLVTEPRKKVVILVTKEAHCIGDILIKAYSGA
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCC
MNIDIAAVVGNHDVLGGLIEKFDIPFHYVSHEGLSREEHEEKMLEVINSYEPEYVVLAKY
CCEEEEEEECCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
MRVLTPNFVAQFPKKIINIHHSFLPAFIGAKPYQQAYDRGVKIIGATAHFVTNDLDEGPI
HHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEHHHHHHCCCCCCCC
IKQDVIPVDHNFSAEDMAMAGRDVEKSVLSKALTKVLNDHVFVYGNKTVIL
CCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCEEEC
>Mature Secondary Structure
MRTELEGIFNDVTFLADIDEALPSGSHRKLVTEPRKKVVILVTKEAHCIGDILIKAYSGA
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCC
MNIDIAAVVGNHDVLGGLIEKFDIPFHYVSHEGLSREEHEEKMLEVINSYEPEYVVLAKY
CCEEEEEEECCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
MRVLTPNFVAQFPKKIINIHHSFLPAFIGAKPYQQAYDRGVKIIGATAHFVTNDLDEGPI
HHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEHHHHHHCCCCCCCC
IKQDVIPVDHNFSAEDMAMAGRDVEKSVLSKALTKVLNDHVFVYGNKTVIL
CCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]