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The map label for this gene is pdxB

Identifier: 59712306

GI number: 59712306

Start: 1914874

End: 1916004

Strand: Reverse

Name: pdxB

Synonym: VF_1699

Alternate gene names: 59712306

Gene position: 1916004-1914874 (Counterclockwise)

Preceding gene: 59712307

Following gene: 59712305

Centisome position: 66.13

GC content: 38.73

Gene sequence:

>1131_bases
ATGAAAATACTGATTGATGAAAATATGCCTTATGCTGCTGAACTGTTTAGTGGATTAGGCGAAGTTATTGCTAAATCAGG
CAGAACATTAACCGCTGACGATCTGATTGATGTTGATGCATTAATGATTCGTTCAGTAACGAAAGTGAATGCTGAATTGA
TTGAAAAAGCCAATAAACTTAAATTTGTTGGTACGGCAACAGCGGGTATGGATCATGTTGATCAAGCATTGTTGAAAGAG
AAAGGCATCTTTTTTACCGCGGCTCCGGGTTGTAACAAAGTAGGGGTGGCAGAGTATGTACTAAGCTGTTTAATGGTATT
AGCTCAACAACATGGTTTTTCTATTTTCGATAAAACCTTTGGTATTGTTGGTGCTGGGCAGGTTGGGTCTTATTTAGCTC
AATGTTTAGATGCTTTGAATATTTCTTATCTTTTGAATGACCCAATTAAAGAGCAAGAGGGCGACTCTCGCTCATTCGTA
GCATTGGATGAATTACTAGAAAAATCAGATGTCATCACTCTTCATACACCGATTACTCGTGATGGTGAATATCCTACGCA
TCACCTCATTGGACAAAAGACATTATCAAAATTACGTAATGATCAGATATTAATTAATGCTGCCCGTGGCCCTGTGGTAG
ATAATCAAGCCTTAAAACAACGCTTGCTAAAACAAGACGGCTTTAAAGCGGTACTGGATGTTTTTGAGTTTGAACCAGAC
GTTGATATGGAATTATTGCCGTTATTATCTTTTGCTACTCCGCATATTGCAGGATATGGATTAGAGGGGAAAGCGCGTGG
TACGACAATGATATTTAACTTTTATTGTGAGTTTTTAAAGCGCAATGAAAGAGCCGACCCTCAAACATTATTACCGATAG
CACCCATTCCGAATGTGACATTAAATCAACAATGGGATCATTCAACACTGCACAATTTGATTCAGTTAGTCTATGATGTG
CGAAAAGATGATGCCGTATTTCGAAAAGAAATCGCAACCGTCGGAGCATTTGATAAAATGCGCAAAGACTATTGGGATAG
AAGAGAATACAGTGCTATCACGATCACAGGGACTCAATGCTGTGATCTTACTCCGTTACAACAACTTGGATTTACAATCG
AGGAAGTCTAA

Upstream 100 bases:

>100_bases
TATACCTATTTTCTGTTATGGAAGTGATGATACCAATTCCATTAATTAGTTACATTGGTATAAACTCAAGCCATAAGTCA
AAAGGATAGATGAGTAAGAG

Downstream 100 bases:

>100_bases
TGACTCAAGAATTTGATGTTGCCATTCTTGGCGCAACTGGTGCCGTTGGCGAAGCAATCGTAGAAGTATTACAAGATCGT
AATTTTCCGGTTCGCAATTT

Product: erythronate-4-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKILIDENMPYAAELFSGLGEVIAKSGRTLTADDLIDVDALMIRSVTKVNAELIEKANKLKFVGTATAGMDHVDQALLKE
KGIFFTAAPGCNKVGVAEYVLSCLMVLAQQHGFSIFDKTFGIVGAGQVGSYLAQCLDALNISYLLNDPIKEQEGDSRSFV
ALDELLEKSDVITLHTPITRDGEYPTHHLIGQKTLSKLRNDQILINAARGPVVDNQALKQRLLKQDGFKAVLDVFEFEPD
VDMELLPLLSFATPHIAGYGLEGKARGTTMIFNFYCEFLKRNERADPQTLLPIAPIPNVTLNQQWDHSTLHNLIQLVYDV
RKDDAVFRKEIATVGAFDKMRKDYWDRREYSAITITGTQCCDLTPLQQLGFTIEEV

Sequences:

>Translated_376_residues
MKILIDENMPYAAELFSGLGEVIAKSGRTLTADDLIDVDALMIRSVTKVNAELIEKANKLKFVGTATAGMDHVDQALLKE
KGIFFTAAPGCNKVGVAEYVLSCLMVLAQQHGFSIFDKTFGIVGAGQVGSYLAQCLDALNISYLLNDPIKEQEGDSRSFV
ALDELLEKSDVITLHTPITRDGEYPTHHLIGQKTLSKLRNDQILINAARGPVVDNQALKQRLLKQDGFKAVLDVFEFEPD
VDMELLPLLSFATPHIAGYGLEGKARGTTMIFNFYCEFLKRNERADPQTLLPIAPIPNVTLNQQWDHSTLHNLIQLVYDV
RKDDAVFRKEIATVGAFDKMRKDYWDRREYSAITITGTQCCDLTPLQQLGFTIEEV
>Mature_376_residues
MKILIDENMPYAAELFSGLGEVIAKSGRTLTADDLIDVDALMIRSVTKVNAELIEKANKLKFVGTATAGMDHVDQALLKE
KGIFFTAAPGCNKVGVAEYVLSCLMVLAQQHGFSIFDKTFGIVGAGQVGSYLAQCLDALNISYLLNDPIKEQEGDSRSFV
ALDELLEKSDVITLHTPITRDGEYPTHHLIGQKTLSKLRNDQILINAARGPVVDNQALKQRLLKQDGFKAVLDVFEFEPD
VDMELLPLLSFATPHIAGYGLEGKARGTTMIFNFYCEFLKRNERADPQTLLPIAPIPNVTLNQQWDHSTLHNLIQLVYDV
RKDDAVFRKEIATVGAFDKMRKDYWDRREYSAITITGTQCCDLTPLQQLGFTIEEV

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily

Homologues:

Organism=Homo sapiens, GI23308577, Length=294, Percent_Identity=30.2721088435374, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI145580578, Length=256, Percent_Identity=28.125, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4557499, Length=256, Percent_Identity=28.125, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI145580575, Length=256, Percent_Identity=28.125, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI61743967, Length=256, Percent_Identity=28.125, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI4557497, Length=256, Percent_Identity=28.125, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1788660, Length=372, Percent_Identity=50.2688172043011, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI87082289, Length=250, Percent_Identity=32.8, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1787645, Length=209, Percent_Identity=26.3157894736842, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789279, Length=258, Percent_Identity=30.6201550387597, Blast_Score=85, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17532191, Length=268, Percent_Identity=30.2238805970149, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322116, Length=303, Percent_Identity=30.03300330033, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320925, Length=303, Percent_Identity=29.7029702970297, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6324055, Length=159, Percent_Identity=30.188679245283, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324964, Length=285, Percent_Identity=24.9122807017544, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921140, Length=258, Percent_Identity=27.906976744186, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24646446, Length=232, Percent_Identity=28.0172413793103, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24646448, Length=232, Percent_Identity=28.0172413793103, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24646452, Length=232, Percent_Identity=28.0172413793103, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24646450, Length=232, Percent_Identity=28.0172413793103, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI62472511, Length=232, Percent_Identity=28.0172413793103, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28574286, Length=243, Percent_Identity=27.1604938271605, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24585514, Length=230, Percent_Identity=26.0869565217391, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI28574282, Length=230, Percent_Identity=26.0869565217391, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI28574284, Length=230, Percent_Identity=26.0869565217391, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI45551003, Length=230, Percent_Identity=26.0869565217391, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45552429, Length=230, Percent_Identity=26.0869565217391, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24585516, Length=272, Percent_Identity=27.2058823529412, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXB_VIBF1 (Q5E452)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205082.1
- HSSP:   P0A9T0
- ProteinModelPortal:   Q5E452
- SMR:   Q5E452
- STRING:   Q5E452
- GeneID:   3277671
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1699
- NMPDR:   fig|312309.3.peg.1699
- eggNOG:   COG0111
- HOGENOM:   HBG289972
- OMA:   SAPGCNA
- PhylomeDB:   Q5E452
- ProtClustDB:   CLSK874680
- BioCyc:   VFIS312309:VF1699-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01825
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10996:SF4

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: =1.1.1.290

Molecular weight: Translated: 41845; Mature: 41845

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 209-209 ACT_SITE 238-238 ACT_SITE 255-255 BINDING 45-45 BINDING 67-67 BINDING 147-147 BINDING 176-176 BINDING 233-233 BINDING 258-258 BINDING 259-259

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILIDENMPYAAELFSGLGEVIAKSGRTLTADDLIDVDALMIRSVTKVNAELIEKANKL
CEEEEECCCCHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
KFVGTATAGMDHVDQALLKEKGIFFTAAPGCNKVGVAEYVLSCLMVLAQQHGFSIFDKTF
EEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
GIVGAGQVGSYLAQCLDALNISYLLNDPIKEQEGDSRSFVALDELLEKSDVITLHTPITR
CCCCCCHHHHHHHHHHHHHCHHHHHCCCCHHCCCCCCHHHHHHHHHHHCCEEEEECCCCC
DGEYPTHHLIGQKTLSKLRNDQILINAARGPVVDNQALKQRLLKQDGFKAVLDVFEFEPD
CCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCC
VDMELLPLLSFATPHIAGYGLEGKARGTTMIFNFYCEFLKRNERADPQTLLPIAPIPNVT
CCHHHHHHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCC
LNQQWDHSTLHNLIQLVYDVRKDDAVFRKEIATVGAFDKMRKDYWDRREYSAITITGTQC
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
CDLTPLQQLGFTIEEV
CCCCCHHHHCCCHHCC
>Mature Secondary Structure
MKILIDENMPYAAELFSGLGEVIAKSGRTLTADDLIDVDALMIRSVTKVNAELIEKANKL
CEEEEECCCCHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
KFVGTATAGMDHVDQALLKEKGIFFTAAPGCNKVGVAEYVLSCLMVLAQQHGFSIFDKTF
EEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
GIVGAGQVGSYLAQCLDALNISYLLNDPIKEQEGDSRSFVALDELLEKSDVITLHTPITR
CCCCCCHHHHHHHHHHHHHCHHHHHCCCCHHCCCCCCHHHHHHHHHHHCCEEEEECCCCC
DGEYPTHHLIGQKTLSKLRNDQILINAARGPVVDNQALKQRLLKQDGFKAVLDVFEFEPD
CCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCC
VDMELLPLLSFATPHIAGYGLEGKARGTTMIFNFYCEFLKRNERADPQTLLPIAPIPNVT
CCHHHHHHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCC
LNQQWDHSTLHNLIQLVYDVRKDDAVFRKEIATVGAFDKMRKDYWDRREYSAITITGTQC
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
CDLTPLQQLGFTIEEV
CCCCCHHHHCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA