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The map label for this gene is truA

Identifier: 59712303

GI number: 59712303

Start: 1908674

End: 1909462

Strand: Reverse

Name: truA

Synonym: VF_1696

Alternate gene names: 59712303

Gene position: 1909462-1908674 (Counterclockwise)

Preceding gene: 59712304

Following gene: 172087721

Centisome position: 65.9

GC content: 40.94

Gene sequence:

>789_bases
ATGCGAATAGCTCTATGTATTGAATATGATGGTGCCGAATATTACGGTTGGCAACGTCAAAGAGATGTAAACAGTGTCCA
AGAAGAACTGGAAAAAGCATTAACGATTGTTGCTAATGAGCCTATTGAAGTTCATTGTGCAGGTCGAACGGATGCTGGTG
TTCACGGTACAGGACAAGTCGTGCACTTTGATACAACAAGTTCTCGCAAATTAGCAGCGTGGATGATGGGAGCGAATGCG
AACTTACCAAAGAACATCGCAGTTCGTTGGGCCAAAGAGGTAAATGAGGATTTTCATGCTCGTTTTACCGCTACTGCTCG
TCGTTATCGCTATATCATCTATAATAATCGTTTACGTCCTGCAATTTTAGGGCATGGTGTAAGCCATTATCATGATGCGT
TAGATGCGAATTTAATGCATGAAGCTGGTCAATATTTATTAGGCGAAAATGATTTTACGTCATTTCGTGCCGTTCACTGT
CAATCACGTTCACCATGGCGCAACTTAATGCACTTAAAAGTCACTCGTCATGGTGATTTTATTGTTATTGATATTAAAGC
AAACGCTTTTGTTCACCATATGGTGCGTAATATTACCGGCAGCTTAATCGAAGTAGGCAAAGGAAAGCAAAAACCAGAAT
GGATTAAATGGTTATTAGAAGCTAAAGACCGAAAATTGGCTGGAGCTACGGCTAAAGCCGAAGGTTTGTATCTTGTGGAT
GTAGATTACCCGCTAGAATGGGAATTACCGCGAGTACCACTAGGTCCTTTATTTCTGGATAATGATTGA

Upstream 100 bases:

>100_bases
AACAAATCGGCTAACTTAAGAAACAATATTAAAAAGGCGGTGGCAGTGAGCTCCGCCTTTTTTTATAATGATGTTTAATT
GAATTAAAAGAAAAGAAATC

Downstream 100 bases:

>100_bases
TTTATCACTACGAAAAGTAGAGATGGATTCAATATTGAAAATAGGTACAACCAGTTTTTTATCTACACTGGCGGATATTT
ATGGTTTAATCTATTTAATT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRIALCIEYDGAEYYGWQRQRDVNSVQEELEKALTIVANEPIEVHCAGRTDAGVHGTGQVVHFDTTSSRKLAAWMMGANA
NLPKNIAVRWAKEVNEDFHARFTATARRYRYIIYNNRLRPAILGHGVSHYHDALDANLMHEAGQYLLGENDFTSFRAVHC
QSRSPWRNLMHLKVTRHGDFIVIDIKANAFVHHMVRNITGSLIEVGKGKQKPEWIKWLLEAKDRKLAGATAKAEGLYLVD
VDYPLEWELPRVPLGPLFLDND

Sequences:

>Translated_262_residues
MRIALCIEYDGAEYYGWQRQRDVNSVQEELEKALTIVANEPIEVHCAGRTDAGVHGTGQVVHFDTTSSRKLAAWMMGANA
NLPKNIAVRWAKEVNEDFHARFTATARRYRYIIYNNRLRPAILGHGVSHYHDALDANLMHEAGQYLLGENDFTSFRAVHC
QSRSPWRNLMHLKVTRHGDFIVIDIKANAFVHHMVRNITGSLIEVGKGKQKPEWIKWLLEAKDRKLAGATAKAEGLYLVD
VDYPLEWELPRVPLGPLFLDND
>Mature_262_residues
MRIALCIEYDGAEYYGWQRQRDVNSVQEELEKALTIVANEPIEVHCAGRTDAGVHGTGQVVHFDTTSSRKLAAWMMGANA
NLPKNIAVRWAKEVNEDFHARFTATARRYRYIIYNNRLRPAILGHGVSHYHDALDANLMHEAGQYLLGENDFTSFRAVHC
QSRSPWRNLMHLKVTRHGDFIVIDIKANAFVHHMVRNITGSLIEVGKGKQKPEWIKWLLEAKDRKLAGATAKAEGLYLVD
VDYPLEWELPRVPLGPLFLDND

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=264, Percent_Identity=30.6818181818182, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1788657, Length=258, Percent_Identity=72.8682170542636, Blast_Score=400, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24648455, Length=234, Percent_Identity=25.6410256410256, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281362146, Length=234, Percent_Identity=25.6410256410256, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922728, Length=265, Percent_Identity=28.6792452830189, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_VIBF1 (Q5E455)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205079.1
- ProteinModelPortal:   Q5E455
- SMR:   Q5E455
- STRING:   Q5E455
- GeneID:   3277668
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1696
- NMPDR:   fig|312309.3.peg.1696
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- PhylomeDB:   Q5E455
- ProtClustDB:   PRK00021
- BioCyc:   VFIS312309:VF1696-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29872; Mature: 29872

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: ACT_SITE 51-51

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALCIEYDGAEYYGWQRQRDVNSVQEELEKALTIVANEPIEVHCAGRTDAGVHGTGQV
CEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCEEEEECCCCCCCCCCCCCE
VHFDTTSSRKLAAWMMGANANLPKNIAVRWAKEVNEDFHARFTATARRYRYIIYNNRLRP
EEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHCCHHHHHEEEEEHEEEEEEEECCCCCH
AILGHGVSHYHDALDANLMHEAGQYLLGENDFTSFRAVHCQSRSPWRNLMHLKVTRHGDF
HHHHCCHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCCCCCHHEEEEEEEECCCE
IVIDIKANAFVHHMVRNITGSLIEVGKGKQKPEWIKWLLEAKDRKLAGATAKAEGLYLVD
EEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEECCCCCCCCEEEEE
VDYPLEWELPRVPLGPLFLDND
ECCCCCCCCCCCCCCCEEECCC
>Mature Secondary Structure
MRIALCIEYDGAEYYGWQRQRDVNSVQEELEKALTIVANEPIEVHCAGRTDAGVHGTGQV
CEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCEEEEECCCCCCCCCCCCCE
VHFDTTSSRKLAAWMMGANANLPKNIAVRWAKEVNEDFHARFTATARRYRYIIYNNRLRP
EEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHCCHHHHHEEEEEHEEEEEEEECCCCCH
AILGHGVSHYHDALDANLMHEAGQYLLGENDFTSFRAVHCQSRSPWRNLMHLKVTRHGDF
HHHHCCHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCCCCCHHEEEEEEEECCCE
IVIDIKANAFVHHMVRNITGSLIEVGKGKQKPEWIKWLLEAKDRKLAGATAKAEGLYLVD
EEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEECCCCCCCCEEEEE
VDYPLEWELPRVPLGPLFLDND
ECCCCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA