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The map label for this gene is ybaZ [H]

Identifier: 59712182

GI number: 59712182

Start: 1763981

End: 1764283

Strand: Direct

Name: ybaZ [H]

Synonym: VF_1575

Alternate gene names: 59712182

Gene position: 1763981-1764283 (Clockwise)

Preceding gene: 59712180

Following gene: 59712184

Centisome position: 60.88

GC content: 34.98

Gene sequence:

>303_bases
ATGAATGAATTTACACAAAGTATTTATATGAACTTAGCGCAGATACCTAAAGGCCGAATCATCAGTTATGGACAATTAGC
AAAACTTGCTGGTTACCCTAACCACTCAAGACACGTAGGAAAAACATTAGCAAAGCTACCAAAAGACACAACTCTACCAT
GGCATCGGGTTGTCAATTCTCAGGGGAGGATTTCGTTATCTGGTGACAGTTTCTATAAACAAAAAGAAAGATTAGAAAAG
GAAGGAATTGCTATTTGTGAGAATGGAAAAATAATGAATTTTAAGCAAGTAATAATGCAATAA

Upstream 100 bases:

>100_bases
CCTCCATGGTTCAGAGTTGTTCACTTTGTTACTATTTCAGTGTAGCGTTATTCATCATCTTTATCACTGACCCACTTCAC
TTTTTATTAAGGCCACATTC

Downstream 100 bases:

>100_bases
CTCACATTACTACTTGCTCTACTTATTCAAATAAGTATCACTTACTCGAATTAAGAATAACCGTTAAATTACTGACGAAC
GCCTTTCAGTAATAGGTCTT

Product: O6-methylguanine-DNA methyltransferase

Products: DNA; protein S-methyl-L-cysteine

Alternate protein names: NA

Number of amino acids: Translated: 100; Mature: 100

Protein sequence:

>100_residues
MNEFTQSIYMNLAQIPKGRIISYGQLAKLAGYPNHSRHVGKTLAKLPKDTTLPWHRVVNSQGRISLSGDSFYKQKERLEK
EGIAICENGKIMNFKQVIMQ

Sequences:

>Translated_100_residues
MNEFTQSIYMNLAQIPKGRIISYGQLAKLAGYPNHSRHVGKTLAKLPKDTTLPWHRVVNSQGRISLSGDSFYKQKERLEK
EGIAICENGKIMNFKQVIMQ
>Mature_100_residues
MNEFTQSIYMNLAQIPKGRIISYGQLAKLAGYPNHSRHVGKTLAKLPKDTTLPWHRVVNSQGRISLSGDSFYKQKERLEK
EGIAICENGKIMNFKQVIMQ

Specific function: Unknown

COG id: COG3695

COG function: function code L; Predicted methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1786659, Length=90, Percent_Identity=44.4444444444444, Blast_Score=92, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014048
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1 [H]

EC number: 2.1.1.63

Molecular weight: Translated: 11392; Mature: 11392

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEFTQSIYMNLAQIPKGRIISYGQLAKLAGYPNHSRHVGKTLAKLPKDTTLPWHRVVNS
CCHHHHHHHHHHHHCCCCCEECHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCC
QGRISLSGDSFYKQKERLEKEGIAICENGKIMNFKQVIMQ
CCCEEECCCHHHHHHHHHHHCCEEEECCCCCCCHHHHHCC
>Mature Secondary Structure
MNEFTQSIYMNLAQIPKGRIISYGQLAKLAGYPNHSRHVGKTLAKLPKDTTLPWHRVVNS
CCHHHHHHHHHHHHCCCCCEECHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCC
QGRISLSGDSFYKQKERLEKEGIAICENGKIMNFKQVIMQ
CCCEEECCCHHHHHHHHHHHCCEEEECCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA containing 6-O-methylguanine; protein L-cysteine

Specific reaction: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]