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The map label for this gene is pleD [H]

Identifier: 59712168

GI number: 59712168

Start: 1746343

End: 1747674

Strand: Reverse

Name: pleD [H]

Synonym: VF_1561

Alternate gene names: 59712168

Gene position: 1747674-1746343 (Counterclockwise)

Preceding gene: 59712169

Following gene: 59712164

Centisome position: 60.32

GC content: 35.89

Gene sequence:

>1332_bases
ATGAGCATTTTTCAAAAAATTGCAGTGGTGATGTTAATACTGTCGACAGGCATTCTTGTTTGGTATCACTTTGATGGTGA
CTCAAAAACATTAACCATCAATCCCGAAAAATATCAATATTATGCAACGGATGATCGTAAAGTTGGTGGTATTAGTGATT
CGGTTTTAGTTCAAACTAATAATAAACCGACAATTAAGTGCGATCTTAAACAAGGCAATTATGCTTGGCCATATTGCGAA
GCTGCAATTCATTTATCAGACAGTATGTTTGAGGGTATTGATCTTTCTGATTATTCAAAGCTTGTTTTAGATATTGATTA
TAAAAACTCGGACCCAAATGGCCGTTTACGAGTTTATTTACGTAATAGTAACCCTGCATATACTAAGCGTAATGACGCGG
CATCGTTGAAATTTAATGGGATTGAGTATGAACCAGGTTTTAATACTGGTCCTAAAGAGATTAGCTTATCTGATTTCCAG
GTACTTACTTGGTGGATAGCGGATTACAAAGTTGCGGTAGAACATGCGTCACCTGAGTTTTCGAATGTTTCTATCATTGA
AATAGCTACCGCTTCAGGAGTAAGCGAAGGTCATTTCGAAATGACTATAAACAGTATTGAATTACGAGGTGTTTGGATTA
AAGAAACAACCTTATTAAAAACGATTCTAGGTGCATGGCTTGTTGTTATTATTGCTTATGTTTTATATGAACAGCGTGCA
TTAAATAAGCGATTAAAAGCCAGTACTTATCGTGAAAGTCGTTTACGTAAATTAAATGATACGTTAAAAGAGAAAAATGT
AGAGTTTGCAGAATTGGCTCATCGAGACTCCTTAACGGGTGCTCGTAACCGTAATGCTGTTCGTGATTGGCTAGAACGTA
TGTCGCAACAAGTTCGTTGGGGCAAGCAAACCTTTTCTGTAATTTATATAGATATTGATTTCTTTAAAAAAGTGAATGAT
GACTTAGGGCATCAAACTGGTGATGATATTTTAAGAGAGTTTGTTTTAGTGATTACTGGAGCAATAAAAAGTACTGATTT
TTTGGTTCGTTGGGGAGGGGAAGAATTTATCGTATTTTCTCCAAATAATGATCTTCAACAGGCAGAAGAAAAAGCAGAGC
TTATACGTCGCACTGTAGAGCATCACTTATGGTGCCATAATGAACGCTTAACATGCAGTCTTGGTGTTGCAGAGATGAAG
GATGAACGGATTACTGAGGTGATTGCTCGTGCTGATGATGCTTTATTTAAAGCTAAGAATGCAGGACGGAACAATGTAAA
AACCAGTTCTAATATTGTTAACTCAAGTTCAAATATACATTATATGAATTAA

Upstream 100 bases:

>100_bases
GATATATCGTATTTTTATTTCTTTGCGATGAAAAATAAGTGGGGTATTATTTTTTTGGAATGAAGATCAAAATAATTAAG
GATTTGCGTAAAGGATTACT

Downstream 100 bases:

>100_bases
GTTCAAACCCTAGCTGCTGAAGCTAGGGTTTTTATATCTAATGGTTTCTGGTGAGTTATTCTTTAGGGAGAGTTCTGTGA
TCAATAGGTTCTTTAGGCCC

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSIFQKIAVVMLILSTGILVWYHFDGDSKTLTINPEKYQYYATDDRKVGGISDSVLVQTNNKPTIKCDLKQGNYAWPYCE
AAIHLSDSMFEGIDLSDYSKLVLDIDYKNSDPNGRLRVYLRNSNPAYTKRNDAASLKFNGIEYEPGFNTGPKEISLSDFQ
VLTWWIADYKVAVEHASPEFSNVSIIEIATASGVSEGHFEMTINSIELRGVWIKETTLLKTILGAWLVVIIAYVLYEQRA
LNKRLKASTYRESRLRKLNDTLKEKNVEFAELAHRDSLTGARNRNAVRDWLERMSQQVRWGKQTFSVIYIDIDFFKKVND
DLGHQTGDDILREFVLVITGAIKSTDFLVRWGGEEFIVFSPNNDLQQAEEKAELIRRTVEHHLWCHNERLTCSLGVAEMK
DERITEVIARADDALFKAKNAGRNNVKTSSNIVNSSSNIHYMN

Sequences:

>Translated_443_residues
MSIFQKIAVVMLILSTGILVWYHFDGDSKTLTINPEKYQYYATDDRKVGGISDSVLVQTNNKPTIKCDLKQGNYAWPYCE
AAIHLSDSMFEGIDLSDYSKLVLDIDYKNSDPNGRLRVYLRNSNPAYTKRNDAASLKFNGIEYEPGFNTGPKEISLSDFQ
VLTWWIADYKVAVEHASPEFSNVSIIEIATASGVSEGHFEMTINSIELRGVWIKETTLLKTILGAWLVVIIAYVLYEQRA
LNKRLKASTYRESRLRKLNDTLKEKNVEFAELAHRDSLTGARNRNAVRDWLERMSQQVRWGKQTFSVIYIDIDFFKKVND
DLGHQTGDDILREFVLVITGAIKSTDFLVRWGGEEFIVFSPNNDLQQAEEKAELIRRTVEHHLWCHNERLTCSLGVAEMK
DERITEVIARADDALFKAKNAGRNNVKTSSNIVNSSSNIHYMN
>Mature_442_residues
SIFQKIAVVMLILSTGILVWYHFDGDSKTLTINPEKYQYYATDDRKVGGISDSVLVQTNNKPTIKCDLKQGNYAWPYCEA
AIHLSDSMFEGIDLSDYSKLVLDIDYKNSDPNGRLRVYLRNSNPAYTKRNDAASLKFNGIEYEPGFNTGPKEISLSDFQV
LTWWIADYKVAVEHASPEFSNVSIIEIATASGVSEGHFEMTINSIELRGVWIKETTLLKTILGAWLVVIIAYVLYEQRAL
NKRLKASTYRESRLRKLNDTLKEKNVEFAELAHRDSLTGARNRNAVRDWLERMSQQVRWGKQTFSVIYIDIDFFKKVNDD
LGHQTGDDILREFVLVITGAIKSTDFLVRWGGEEFIVFSPNNDLQQAEEKAELIRRTVEHHLWCHNERLTCSLGVAEMKD
ERITEVIARADDALFKAKNAGRNNVKTSSNIVNSSSNIHYMN

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=36.6279069767442, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787262, Length=162, Percent_Identity=37.037037037037, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082007, Length=166, Percent_Identity=36.144578313253, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1788381, Length=159, Percent_Identity=33.9622641509434, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787541, Length=158, Percent_Identity=34.8101265822785, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI145693134, Length=160, Percent_Identity=33.125, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787802, Length=165, Percent_Identity=30.9090909090909, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787816, Length=166, Percent_Identity=30.7228915662651, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=35.1515151515151, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081974, Length=150, Percent_Identity=27.3333333333333, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=27.1676300578035, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081977, Length=167, Percent_Identity=29.3413173652695, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 50592; Mature: 50461

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIFQKIAVVMLILSTGILVWYHFDGDSKTLTINPEKYQYYATDDRKVGGISDSVLVQTN
CCHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCCEEEEECCCCCCCCCCCCEEEEEC
NKPTIKCDLKQGNYAWPYCEAAIHLSDSMFEGIDLSDYSKLVLDIDYKNSDPNGRLRVYL
CCCEEEEEECCCCCCCCHHHHEEEECHHHHCCCCCCCCCEEEEEEEECCCCCCCEEEEEE
RNSNPAYTKRNDAASLKFNGIEYEPGFNTGPKEISLSDFQVLTWWIADYKVAVEHASPEF
ECCCCCCCCCCCCCEEEECCEEECCCCCCCCCCEECCCCEEEEEEECCEEEEEEECCCCC
SNVSIIEIATASGVSEGHFEMTINSIELRGVWIKETTLLKTILGAWLVVIIAYVLYEQRA
CCEEEEEEEECCCCCCCEEEEEEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
LNKRLKASTYRESRLRKLNDTLKEKNVEFAELAHRDSLTGARNRNAVRDWLERMSQQVRW
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHC
GKQTFSVIYIDIDFFKKVNDDLGHQTGDDILREFVLVITGAIKSTDFLVRWGGEEFIVFS
CCCEEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEC
PNNDLQQAEEKAELIRRTVEHHLWCHNERLTCSLGVAEMKDERITEVIARADDALFKAKN
CCCCHHHHHHHHHHHHHHHHHHHEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCC
AGRNNVKTSSNIVNSSSNIHYMN
CCCCCCCCCCHHCCCCCCEEECC
>Mature Secondary Structure 
SIFQKIAVVMLILSTGILVWYHFDGDSKTLTINPEKYQYYATDDRKVGGISDSVLVQTN
CHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCCEEEEECCCCCCCCCCCCEEEEEC
NKPTIKCDLKQGNYAWPYCEAAIHLSDSMFEGIDLSDYSKLVLDIDYKNSDPNGRLRVYL
CCCEEEEEECCCCCCCCHHHHEEEECHHHHCCCCCCCCCEEEEEEEECCCCCCCEEEEEE
RNSNPAYTKRNDAASLKFNGIEYEPGFNTGPKEISLSDFQVLTWWIADYKVAVEHASPEF
ECCCCCCCCCCCCCEEEECCEEECCCCCCCCCCEECCCCEEEEEEECCEEEEEEECCCCC
SNVSIIEIATASGVSEGHFEMTINSIELRGVWIKETTLLKTILGAWLVVIIAYVLYEQRA
CCEEEEEEEECCCCCCCEEEEEEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
LNKRLKASTYRESRLRKLNDTLKEKNVEFAELAHRDSLTGARNRNAVRDWLERMSQQVRW
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHC
GKQTFSVIYIDIDFFKKVNDDLGHQTGDDILREFVLVITGAIKSTDFLVRWGGEEFIVFS
CCCEEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEC
PNNDLQQAEEKAELIRRTVEHHLWCHNERLTCSLGVAEMKDERITEVIARADDALFKAKN
CCCCHHHHHHHHHHHHHHHHHHHEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCC
AGRNNVKTSSNIVNSSSNIHYMN
CCCCCCCCCCHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA