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The map label for this gene is ansB [H]

Identifier: 59712134

GI number: 59712134

Start: 1709434

End: 1710495

Strand: Reverse

Name: ansB [H]

Synonym: VF_1527

Alternate gene names: 59712134

Gene position: 1710495-1709434 (Counterclockwise)

Preceding gene: 59712137

Following gene: 59712132

Centisome position: 59.03

GC content: 39.55

Gene sequence:

>1062_bases
ATGAAAAAGAATGCCATTGCAGTAGGTATGTTGCTTTCTGGTCTTTGCTTTAGCTCAATCAGTCTTGCTGATGATTTACC
AAATATTAAAATCTTAGCGACTGGCGGTACTATTGCTGGCGCAGGTCAATCCGCAACAGGTTCTAGCTATACATCTGGTA
AAGTTGGAGTCGATTCTTTGATTCATGCTGTACCTCAAATGACAGAAATTGCGGATATTAGTGGTGAACAAGTCGTAAGT
ATCGGTTCTCAAGATATGAATGATGAGGTATGGCTAACATTATCAAAGCGTGTAAATGAATTATTAGCTCAAGATGATGT
TGATGGTGTTGTGATTACTCATGGCACAGATACGATGGAAGAAACTGCTTATTTCCTAGACCTTACTGTTAAAAGTGATA
AACCTGTAATTTTGGTTGGTGCAATGCGACCATCAACAGCAATGAGTGCCGATGGTCCGGTCAATTTATATAATGCGGTT
GTTGCGGCAACGGATGAAGATTCTGTAGGGCGTGGTGTATTGGTTGCAATGAATGATACGGTATTTGATGCTCGTGATGT
AACCAAAACCAATACAACGTCTGTAAATACGTTTCAGTCACCAAATTTTGGGCCTCTAGGCTATATTCACAATGGTGATG
TTCAATATCAACGTAGCCCTGAACGTAAGCATACAACTGAAGCTGAATTTGATGTATCAAAGTTAAAAACCTTACCTAAG
GTTGGTATTGTGTACAACTATGCGAATGCATCAGCATTACCTGTAACAGCAATGGTTGATGCAAAATATGATGGTATCGT
GAGTGCGGGTGTAGGTAATGGTAATATTTATCACTCGGTATTTGATGAGTTAGCTAAAGCAAGCAAAGAAGGGGTGGTGG
TTGTACGTAGTGCTCGCACTGGTTCAGGTTCAACAACGCTTGATGCTGAAATAGATGATGCGAAATACGGTTTTGTTGCG
TCGGGTACATTAAACCCTCAAAAAGCTCGAATTTTATTGATGTTGTCGTTAACTGAAACGGAAAACTACAAAGAAATTCA
AAAGATGTTTCAGTATTACTAG

Upstream 100 bases:

>100_bases
GTTATCACGGTACATTAATTAAAAGCAAATATGATGAATTAAATCAAAATATGTAATTTTATTTTCTCGTAATTAATTGT
TTTTATAATGGAGTACTAAC

Downstream 100 bases:

>100_bases
AAGCATATAGTTTCGTATTTTAAATAAAAAAACAGCCAATATTAATTATTGGCTGTTTTTTTATTTGGAAGGATGAAATG
TTTATGTAGGTTGGTATTAA

Product: L-asparaginase II

Products: NA

Alternate protein names: L-asparaginase II; L-ASNase II; L-asparagine amidohydrolase II; Colaspase [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKKNAIAVGMLLSGLCFSSISLADDLPNIKILATGGTIAGAGQSATGSSYTSGKVGVDSLIHAVPQMTEIADISGEQVVS
IGSQDMNDEVWLTLSKRVNELLAQDDVDGVVITHGTDTMEETAYFLDLTVKSDKPVILVGAMRPSTAMSADGPVNLYNAV
VAATDEDSVGRGVLVAMNDTVFDARDVTKTNTTSVNTFQSPNFGPLGYIHNGDVQYQRSPERKHTTEAEFDVSKLKTLPK
VGIVYNYANASALPVTAMVDAKYDGIVSAGVGNGNIYHSVFDELAKASKEGVVVVRSARTGSGSTTLDAEIDDAKYGFVA
SGTLNPQKARILLMLSLTETENYKEIQKMFQYY

Sequences:

>Translated_353_residues
MKKNAIAVGMLLSGLCFSSISLADDLPNIKILATGGTIAGAGQSATGSSYTSGKVGVDSLIHAVPQMTEIADISGEQVVS
IGSQDMNDEVWLTLSKRVNELLAQDDVDGVVITHGTDTMEETAYFLDLTVKSDKPVILVGAMRPSTAMSADGPVNLYNAV
VAATDEDSVGRGVLVAMNDTVFDARDVTKTNTTSVNTFQSPNFGPLGYIHNGDVQYQRSPERKHTTEAEFDVSKLKTLPK
VGIVYNYANASALPVTAMVDAKYDGIVSAGVGNGNIYHSVFDELAKASKEGVVVVRSARTGSGSTTLDAEIDDAKYGFVA
SGTLNPQKARILLMLSLTETENYKEIQKMFQYY
>Mature_353_residues
MKKNAIAVGMLLSGLCFSSISLADDLPNIKILATGGTIAGAGQSATGSSYTSGKVGVDSLIHAVPQMTEIADISGEQVVS
IGSQDMNDEVWLTLSKRVNELLAQDDVDGVVITHGTDTMEETAYFLDLTVKSDKPVILVGAMRPSTAMSADGPVNLYNAV
VAATDEDSVGRGVLVAMNDTVFDARDVTKTNTTSVNTFQSPNFGPLGYIHNGDVQYQRSPERKHTTEAEFDVSKLKTLPK
VGIVYNYANASALPVTAMVDAKYDGIVSAGVGNGNIYHSVFDELAKASKEGVVVVRSARTGSGSTTLDAEIDDAKYGFVA
SGTLNPQKARILLMLSLTETENYKEIQKMFQYY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=230, Percent_Identity=30, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1789327, Length=353, Percent_Identity=69.6883852691218, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI1788065, Length=292, Percent_Identity=30.1369863013699, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI193207372, Length=229, Percent_Identity=26.2008733624454, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17558280, Length=228, Percent_Identity=26.3157894736842, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17558278, Length=228, Percent_Identity=26.3157894736842, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI193207374, Length=228, Percent_Identity=26.3157894736842, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6323189, Length=333, Percent_Identity=42.9429429429429, Blast_Score=229, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6323187, Length=333, Percent_Identity=42.9429429429429, Blast_Score=229, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6323186, Length=333, Percent_Identity=42.9429429429429, Blast_Score=229, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6323184, Length=333, Percent_Identity=42.9429429429429, Blast_Score=229, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6320527, Length=336, Percent_Identity=38.0952380952381, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24645530, Length=208, Percent_Identity=31.25, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37561; Mature: 37561

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00445 FGGY_KINASES_2 ; PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNAIAVGMLLSGLCFSSISLADDLPNIKILATGGTIAGAGQSATGSSYTSGKVGVDSL
CCCCCEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEECCCCCCCCCCCCCCCCCHHHH
IHAVPQMTEIADISGEQVVSIGSQDMNDEVWLTLSKRVNELLAQDDVDGVVITHGTDTME
HHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEHHHHHHHHHHCCCCCEEEEECCCCCHH
ETAYFLDLTVKSDKPVILVGAMRPSTAMSADGPVNLYNAVVAATDEDSVGRGVLVAMNDT
HCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEEEECCC
VFDARDVTKTNTTSVNTFQSPNFGPLGYIHNGDVQYQRSPERKHTTEAEFDVSKLKTLPK
EECCCCCCCCCCCEEEECCCCCCCCCCEEECCCEEECCCCCCCCCCCCCCCHHHHHCCCC
VGIVYNYANASALPVTAMVDAKYDGIVSAGVGNGNIYHSVFDELAKASKEGVVVVRSART
EEEEEEECCCCCCEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCC
GSGSTTLDAEIDDAKYGFVASGTLNPQKARILLMLSLTETENYKEIQKMFQYY
CCCCEEEEEECCCCCCCEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKKNAIAVGMLLSGLCFSSISLADDLPNIKILATGGTIAGAGQSATGSSYTSGKVGVDSL
CCCCCEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEECCCCCCCCCCCCCCCCCHHHH
IHAVPQMTEIADISGEQVVSIGSQDMNDEVWLTLSKRVNELLAQDDVDGVVITHGTDTME
HHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEHHHHHHHHHHCCCCCEEEEECCCCCHH
ETAYFLDLTVKSDKPVILVGAMRPSTAMSADGPVNLYNAVVAATDEDSVGRGVLVAMNDT
HCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEEEECCC
VFDARDVTKTNTTSVNTFQSPNFGPLGYIHNGDVQYQRSPERKHTTEAEFDVSKLKTLPK
EECCCCCCCCCCCEEEECCCCCCCCCCEEECCCEEECCCCCCCCCCCCCCCHHHHHCCCC
VGIVYNYANASALPVTAMVDAKYDGIVSAGVGNGNIYHSVFDELAKASKEGVVVVRSART
EEEEEEECCCCCCEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCC
GSGSTTLDAEIDDAKYGFVASGTLNPQKARILLMLSLTETENYKEIQKMFQYY
CCCCEEEEEECCCCCCCEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2407723; 2144836; 9278503; 6766894; 387570; 321449; 4561256; 1906013; 1521538; 1287659; 8434007; 8706862 [H]