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The map label for this gene is gatA [H]

Identifier: 59712093

GI number: 59712093

Start: 1644266

End: 1645429

Strand: Reverse

Name: gatA [H]

Synonym: VF_1486

Alternate gene names: 59712093

Gene position: 1645429-1644266 (Counterclockwise)

Preceding gene: 59712096

Following gene: 59712087

Centisome position: 56.79

GC content: 39.6

Gene sequence:

>1164_bases
ATGGATGAGCAAACCATGTATGTCTCTCATGAAGTTGAGTCACAACAATGGGCGTTTTATGGCCTTTTAGAAAATAAAAA
ATTTGTCGTAAGTGATTGCATTGATATAAAAAACACGCCAAATGGCCAAGGTAATCCAACGTGGTTATTAGAGCAAAGAA
AAGCGAGAGAGAACGCACCTGCCGTTGATCTTATATTAAAAAAAGGCGCTATATTCGTTGGTAAAACTCAAATGGACGAA
TTTGGGTTTGGTCTACATGGACACAATCCACACTACGGCCCAATACCAAATAAAAACAGCGAAGGGCGCTTTATTGGCGG
CTCATCGAGTGGTGCAGTAGCAGCAGTGGTTAATGGCGATGCAGATATTGGTTTAGGTATTGATTTTGGTGGTGGTATCC
GTGTTCCTGCTTTATATGCTGGTTTATATGGTTTTAAAGCATCAGCCAGTGCAGTAAACATGCAAGGTATCGATTGTATT
GCAAAAGAAGATACTTCATTGGGGTGGGTCGCAAAAAATTTATCAGATATACGAAAAGTGGCAACCGTGCTTACCCCGAT
GTCTCCATTGGTGACGGTGGAACGTATTGTCGTACTTGATAGCTTATTTGCAGATATTCCAGAAGAAGCGAAAACAAAGT
TAGAGACATTAATTAAATCTTCTCCTTATGAAGTGATACGCAGCAAATCGATCAGTAAAATTATCACCACTAAGGCGGCA
GAGAGCTTCAAAGTGCTTTCAGCAACGCGAGGTTTGCGTAATTTAGAACTGTGGTATGAAAAGAACAAAGCGGCAGTAGG
TAATGAAATAAAGATGCAGATGAAGTGGCTTGCATCTTTAAAATATAAAGATGAAAGGATCGCTTTAGAGCAAAGAGAGT
TAGTGATCTCTGTTTTAGAATCCATCTTAGATGAAAAAACACTGCTACTTATGCCAACAACAGCCAATATTGCACCACCA
ATCACGGCAACGGATGAGCAATTATATAAATTGAATGCTCAAATTTTAAAATACACCTCGTTAGCAAGTTTGGCGGATTT
ACCTCAGTTGCATTTACCTTGGTTTACGGTAAATGATTCTCCTTGGGGGATTTCATTAGTTGGGCAAAAAGGAATGGATA
GACAACTGATTGATGTGGCTATTCGTTGGAAAGGCAATCAATAG

Upstream 100 bases:

>100_bases
CACGTAACTGATAGTTTTCTTGATAATGAGAATGAGTGAAATTGTATTCATTTATGTGACTGGTATGCTGCATTTTTGAA
ATTAAACAGTACGCTGAATT

Downstream 100 bases:

>100_bases
ACATTAAAAATGTTAATCAAATAAAAAGGAGGCCAATAAAGCCTCCTTTTAAGTCATCTATTTAAAGAGTAAACCCTGAT
TCTTATAAAGAAGCGTATTC

Product: glutamyl-tRNA(Gln) amidotransferase, subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MDEQTMYVSHEVESQQWAFYGLLENKKFVVSDCIDIKNTPNGQGNPTWLLEQRKARENAPAVDLILKKGAIFVGKTQMDE
FGFGLHGHNPHYGPIPNKNSEGRFIGGSSSGAVAAVVNGDADIGLGIDFGGGIRVPALYAGLYGFKASASAVNMQGIDCI
AKEDTSLGWVAKNLSDIRKVATVLTPMSPLVTVERIVVLDSLFADIPEEAKTKLETLIKSSPYEVIRSKSISKIITTKAA
ESFKVLSATRGLRNLELWYEKNKAAVGNEIKMQMKWLASLKYKDERIALEQRELVISVLESILDEKTLLLMPTTANIAPP
ITATDEQLYKLNAQILKYTSLASLADLPQLHLPWFTVNDSPWGISLVGQKGMDRQLIDVAIRWKGNQ

Sequences:

>Translated_387_residues
MDEQTMYVSHEVESQQWAFYGLLENKKFVVSDCIDIKNTPNGQGNPTWLLEQRKARENAPAVDLILKKGAIFVGKTQMDE
FGFGLHGHNPHYGPIPNKNSEGRFIGGSSSGAVAAVVNGDADIGLGIDFGGGIRVPALYAGLYGFKASASAVNMQGIDCI
AKEDTSLGWVAKNLSDIRKVATVLTPMSPLVTVERIVVLDSLFADIPEEAKTKLETLIKSSPYEVIRSKSISKIITTKAA
ESFKVLSATRGLRNLELWYEKNKAAVGNEIKMQMKWLASLKYKDERIALEQRELVISVLESILDEKTLLLMPTTANIAPP
ITATDEQLYKLNAQILKYTSLASLADLPQLHLPWFTVNDSPWGISLVGQKGMDRQLIDVAIRWKGNQ
>Mature_387_residues
MDEQTMYVSHEVESQQWAFYGLLENKKFVVSDCIDIKNTPNGQGNPTWLLEQRKARENAPAVDLILKKGAIFVGKTQMDE
FGFGLHGHNPHYGPIPNKNSEGRFIGGSSSGAVAAVVNGDADIGLGIDFGGGIRVPALYAGLYGFKASASAVNMQGIDCI
AKEDTSLGWVAKNLSDIRKVATVLTPMSPLVTVERIVVLDSLFADIPEEAKTKLETLIKSSPYEVIRSKSISKIITTKAA
ESFKVLSATRGLRNLELWYEKNKAAVGNEIKMQMKWLASLKYKDERIALEQRELVISVLESILDEKTLLLMPTTANIAPP
ITATDEQLYKLNAQILKYTSLASLADLPQLHLPWFTVNDSPWGISLVGQKGMDRQLIDVAIRWKGNQ

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI17543272, Length=134, Percent_Identity=33.5820895522388, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=389, Percent_Identity=27.5064267352185, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323950, Length=412, Percent_Identity=26.9417475728155, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 42607; Mature: 42607

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEQTMYVSHEVESQQWAFYGLLENKKFVVSDCIDIKNTPNGQGNPTWLLEQRKARENAP
CCCCEEEEEECCCCCCEEEEEEECCCEEEEEEEECCCCCCCCCCCCEEEEEHHHHCCCCC
AVDLILKKGAIFVGKTQMDEFGFGLHGHNPHYGPIPNKNSEGRFIGGSSSGAVAAVVNGD
CEEEEEECCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEEEECC
ADIGLGIDFGGGIRVPALYAGLYGFKASASAVNMQGIDCIAKEDTSLGWVAKNLSDIRKV
CCEEEEEECCCCCCHHHHHHHHHCCCCCCCEECCCCCEEEECCCCCHHHHHHHHHHHHHH
ATVLTPMSPLVTVERIVVLDSLFADIPEEAKTKLETLIKSSPYEVIRSKSISKIITTKAA
HHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ESFKVLSATRGLRNLELWYEKNKAAVGNEIKMQMKWLASLKYKDERIALEQRELVISVLE
HHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
SILDEKTLLLMPTTANIAPPITATDEQLYKLNAQILKYTSLASLADLPQLHLPWFTVNDS
HHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCC
PWGISLVGQKGMDRQLIDVAIRWKGNQ
CCCEEEECCCCCCCEEEEEEEEECCCC
>Mature Secondary Structure
MDEQTMYVSHEVESQQWAFYGLLENKKFVVSDCIDIKNTPNGQGNPTWLLEQRKARENAP
CCCCEEEEEECCCCCCEEEEEEECCCEEEEEEEECCCCCCCCCCCCEEEEEHHHHCCCCC
AVDLILKKGAIFVGKTQMDEFGFGLHGHNPHYGPIPNKNSEGRFIGGSSSGAVAAVVNGD
CEEEEEECCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEEEECC
ADIGLGIDFGGGIRVPALYAGLYGFKASASAVNMQGIDCIAKEDTSLGWVAKNLSDIRKV
CCEEEEEECCCCCCHHHHHHHHHCCCCCCCEECCCCCEEEECCCCCHHHHHHHHHHHHHH
ATVLTPMSPLVTVERIVVLDSLFADIPEEAKTKLETLIKSSPYEVIRSKSISKIITTKAA
HHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ESFKVLSATRGLRNLELWYEKNKAAVGNEIKMQMKWLASLKYKDERIALEQRELVISVLE
HHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
SILDEKTLLLMPTTANIAPPITATDEQLYKLNAQILKYTSLASLADLPQLHLPWFTVNDS
HHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCC
PWGISLVGQKGMDRQLIDVAIRWKGNQ
CCCEEEECCCCCCCEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]