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The map label for this gene is leuO [H]

Identifier: 59712026

GI number: 59712026

Start: 1570372

End: 1571286

Strand: Reverse

Name: leuO [H]

Synonym: VF_1419

Alternate gene names: 59712026

Gene position: 1571286-1570372 (Counterclockwise)

Preceding gene: 59712027

Following gene: 59712024

Centisome position: 54.23

GC content: 38.03

Gene sequence:

>915_bases
GTGTCGGTTATTATGAAAAGCACAGAACTAAACTTGATCCCTATTTTTGTCGCCATCTATGAAGAGAAAAACCTCTCAAA
GGCGGCAATAAGAATGAATATTAGCCAACCTGCGGTGAGTAAAGCATTAAAACGTCTACGAGAAATCTATGATGATGCAC
TTTTTCATCGAAATACGACGGGAGTAGAACCCACCGTTTTTGCTTCAGATATTTACCCCGCAATGTCTGCAGCACTTAAG
AATTTTACTTCTACGTTGTCAGCATCAAGAGAATTTGATCCTAAGACATCCAATCGAATTTTCTCAATTGCGGTTGTGTC
TACGGTGAATTATTCATTAATACCAAAATTGGTGAAACAGCTTCGTTCATCTGCACCTAATATTTCGTTAGAAATACATC
CTCAATTTACTGAAGATCTAGAGTCAGACTTACGTTTACAGCGTTACGATCTGGTGATTGATATGGCTATTCGTGGACGC
ACTATTTTAAAATCTGAGGTAGTTTATACTGAAAGATTAAGAGTCGTATGCAGTAAAGATCACCCAAGAATAGGGGATTC
AATTAGCCTTGAACAATTCTTAAGTGAAGAGCATGTTGCCGCGTCTCGTTGGCATAGCCGAAGCAGCTTATTGAATGCTG
AAGATATTGGAGAATTAGAAGCTCGTAATATTGTTATTCGAGCAGCTGGTGCCTTTGAAATGATGCCAATTATTGGGTCT
ACTGAGATGATTGGGATGTTACCTCAGTCATCTATTGATGATTTAGGCGATTACTACAATTTGAAATCTTTAGATTTACC
TTTGAATCGTCAACAACACGATTTATGTAGCATTTGGCATCCAAGTCGAAGTAATGATAGTGGCCATCGTTGGTTGCGAC
AACAAATTCAAAAAGCAGCTAAAAATGTCTTTTAA

Upstream 100 bases:

>100_bases
AATAAGCAAAACGAAAAAGTAAGATAATCAACACAGAACTGATGGACTGCACTTGTTTCATCTGCTCAGTTATATAAAGT
GAAATCAGGATGAATCAGCC

Downstream 100 bases:

>100_bases
ACAAACAGCGCTGAATTTTCGGCGCTGTTTTTATTTGTGAAGAATTAATTTTGAATGAAGACTTTTAGTTAGTTTAGTTT
TTATCTCTTACTAAACGTAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSVIMKSTELNLIPIFVAIYEEKNLSKAAIRMNISQPAVSKALKRLREIYDDALFHRNTTGVEPTVFASDIYPAMSAALK
NFTSTLSASREFDPKTSNRIFSIAVVSTVNYSLIPKLVKQLRSSAPNISLEIHPQFTEDLESDLRLQRYDLVIDMAIRGR
TILKSEVVYTERLRVVCSKDHPRIGDSISLEQFLSEEHVAASRWHSRSSLLNAEDIGELEARNIVIRAAGAFEMMPIIGS
TEMIGMLPQSSIDDLGDYYNLKSLDLPLNRQQHDLCSIWHPSRSNDSGHRWLRQQIQKAAKNVF

Sequences:

>Translated_304_residues
MSVIMKSTELNLIPIFVAIYEEKNLSKAAIRMNISQPAVSKALKRLREIYDDALFHRNTTGVEPTVFASDIYPAMSAALK
NFTSTLSASREFDPKTSNRIFSIAVVSTVNYSLIPKLVKQLRSSAPNISLEIHPQFTEDLESDLRLQRYDLVIDMAIRGR
TILKSEVVYTERLRVVCSKDHPRIGDSISLEQFLSEEHVAASRWHSRSSLLNAEDIGELEARNIVIRAAGAFEMMPIIGS
TEMIGMLPQSSIDDLGDYYNLKSLDLPLNRQQHDLCSIWHPSRSNDSGHRWLRQQIQKAAKNVF
>Mature_303_residues
SVIMKSTELNLIPIFVAIYEEKNLSKAAIRMNISQPAVSKALKRLREIYDDALFHRNTTGVEPTVFASDIYPAMSAALKN
FTSTLSASREFDPKTSNRIFSIAVVSTVNYSLIPKLVKQLRSSAPNISLEIHPQFTEDLESDLRLQRYDLVIDMAIRGRT
ILKSEVVYTERLRVVCSKDHPRIGDSISLEQFLSEEHVAASRWHSRSSLLNAEDIGELEARNIVIRAAGAFEMMPIIGST
EMIGMLPQSSIDDLGDYYNLKSLDLPLNRQQHDLCSIWHPSRSNDSGHRWLRQQIQKAAKNVF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=299, Percent_Identity=27.0903010033445, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1786820, Length=265, Percent_Identity=27.5471698113208, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI87081766, Length=273, Percent_Identity=24.9084249084249, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790147, Length=307, Percent_Identity=23.1270358306189, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789204, Length=214, Percent_Identity=28.0373831775701, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI87081904, Length=188, Percent_Identity=23.4042553191489, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693105, Length=174, Percent_Identity=24.7126436781609, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34406; Mature: 34275

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIMKSTELNLIPIFVAIYEEKNLSKAAIRMNISQPAVSKALKRLREIYDDALFHRNTT
CCEEEECCCCCEEEEEEEEECCCCCCEEHEEECCCCHHHHHHHHHHHHHHHHHHHCCCCC
GVEPTVFASDIYPAMSAALKNFTSTLSASREFDPKTSNRIFSIAVVSTVNYSLIPKLVKQ
CCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHCCHHHHHHHHHH
LRSSAPNISLEIHPQFTEDLESDLRLQRYDLVIDMAIRGRTILKSEVVYTERLRVVCSKD
HHHCCCCEEEEECCCHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
HPRIGDSISLEQFLSEEHVAASRWHSRSSLLNAEDIGELEARNIVIRAAGAFEMMPIIGS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHCEEEEEECCCEEEEECCCC
TEMIGMLPQSSIDDLGDYYNLKSLDLPLNRQQHDLCSIWHPSRSNDSGHRWLRQQIQKAA
HHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHH
KNVF
HCCC
>Mature Secondary Structure 
SVIMKSTELNLIPIFVAIYEEKNLSKAAIRMNISQPAVSKALKRLREIYDDALFHRNTT
CEEEECCCCCEEEEEEEEECCCCCCEEHEEECCCCHHHHHHHHHHHHHHHHHHHCCCCC
GVEPTVFASDIYPAMSAALKNFTSTLSASREFDPKTSNRIFSIAVVSTVNYSLIPKLVKQ
CCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHCCHHHHHHHHHH
LRSSAPNISLEIHPQFTEDLESDLRLQRYDLVIDMAIRGRTILKSEVVYTERLRVVCSKD
HHHCCCCEEEEECCCHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
HPRIGDSISLEQFLSEEHVAASRWHSRSSLLNAEDIGELEARNIVIRAAGAFEMMPIIGS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHCEEEEEECCCEEEEECCCC
TEMIGMLPQSSIDDLGDYYNLKSLDLPLNRQQHDLCSIWHPSRSNDSGHRWLRQQIQKAA
HHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHH
KNVF
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3413113; 1630901; 9278503; 3519576 [H]