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The map label for this gene is mdtK

Identifier: 59712022

GI number: 59712022

Start: 1565462

End: 1566844

Strand: Direct

Name: mdtK

Synonym: VF_1415

Alternate gene names: 59712022

Gene position: 1565462-1566844 (Clockwise)

Preceding gene: 172087708

Following gene: 59712025

Centisome position: 54.03

GC content: 41.0

Gene sequence:

>1383_bases
GTGCACAGATACCAAAAAGAAATCGTTCGTTTAATTAAACTCTCTACACCTGTATTAATTGCAAGTGTGGCACAAACAGG
CATGGGCTTTGTTGATACAGTAATGGCTGGTGGCGTAAGCGCAACTGACATGGCTGCTGTAGCCATTGCTGCAAGTGTGT
GGCTACCTTCTGTATTGTTTGGTGTTGGTCTACTTATGGCCCTTGTGCCTGTTGTAGCTCAACTAAACGGTTCAGGAAAA
TCAAAAAAAGTACCATTTGAGATCCACCAAGGTGTTTACCTTGCTTTATTAACCTCAATTCCAATTATGCTGGTGTTATA
TAATGCTGGCTTTATTATTGCCGCAATGGATGTAGAACCTGAGCTATACGAAAAAACTCAAGGCTATTTACATGCTGTAT
TGTGGGCTGCGCCAGCATTTTTATTATTCCAAACTCTGCGTAGTTTTTGTGAAGGTTTATCTCTAACCACTCCAGCAATG
ATTATCGGCTTCATTGGTCTTGCTGCTAACGTTCCATTAAACTGGATGTTTGTTTACGGTGAGCTTGGCGCTCCTGCTCT
TGGCGGTGTTGGTTGTGGTGTTGCTACTGCTATCGTTTATTGGTTGATGTTCTTAACCATGACTTTGTATACGTTTATCG
CTCCGAAACTGCGTCGCGTAAACCTATATGAAAACTGGAATAAACCACAAAGAAAAGAGATCTATCGCCTTTTCAAACTG
GGCCTTCCTGTTGCTTTGTCTATTTTCTTTGAGGTGACCCTATTTGCAGCTGTTGCATTACTGGTTTCTCCGTTAGGCTC
TACGGTAGTTGCCGCTCACCAAGTTGCGATTAACTTTTCATCATTAATTTTTATGATCCCGATGAGTATCGCTGTTGCGG
TAAGTATTCGAGTTGGTCATAAATTAGGTGAGAAAGATCTCGATGGAGCAAAAGTAGCAAGTTATTGCGGTCTAGCTTTT
GGTTTGTTGATGGCATGTTGTACAGCTATTCTAACTCTTATTTTCAGAGAACAAATTGCTTATTTGTATTCAGATAACCA
AGAAGTAATTACCTTAGCGGTAAGTTTAATGCTATTAGCTGCTATTTATCAATGTACTGATGCCGTGCAAGTTGTTGCAG
CTGGTGCCCTACGTGGTTATAAAGACATGAATGCCATCTTTAAGTGTACTTTTGTCTCTTACTGGATAGTTGGTCTGCCT
TCAGGTTATGTTCTTGGCATGACAGATTGGATTAGAGAACCAATGGGCGTTTATGGTTTTTGGTTTGGCTTTATTGGTGG
CTTAACGACATCTGCAATCCTACTAACTTGTCGCCTACTGTGGCTTCAACGTAATCCTACTCGTATCGATATGGAAGTAG
ATGAACTGCAAATCATGCACTAA

Upstream 100 bases:

>100_bases
AAAGCACTCAATCAGACCTTTTTTCTTCATTACATCCTTATGTGATGATGAATCGATAAATTAAACTCCTTATTTACAAA
TATATTGATACGGTGACGCA

Downstream 100 bases:

>100_bases
CTTTCCATTCTAAAATAATAAAAAAGCCGAACATATTAGATATGTTCGACTTTTTTTTAATCAAATACTTACATTAATTG
TTATTACTTACGTTTCTCTT

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter; Na(+)/drug antiporter

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MHRYQKEIVRLIKLSTPVLIASVAQTGMGFVDTVMAGGVSATDMAAVAIAASVWLPSVLFGVGLLMALVPVVAQLNGSGK
SKKVPFEIHQGVYLALLTSIPIMLVLYNAGFIIAAMDVEPELYEKTQGYLHAVLWAAPAFLLFQTLRSFCEGLSLTTPAM
IIGFIGLAANVPLNWMFVYGELGAPALGGVGCGVATAIVYWLMFLTMTLYTFIAPKLRRVNLYENWNKPQRKEIYRLFKL
GLPVALSIFFEVTLFAAVALLVSPLGSTVVAAHQVAINFSSLIFMIPMSIAVAVSIRVGHKLGEKDLDGAKVASYCGLAF
GLLMACCTAILTLIFREQIAYLYSDNQEVITLAVSLMLLAAIYQCTDAVQVVAAGALRGYKDMNAIFKCTFVSYWIVGLP
SGYVLGMTDWIREPMGVYGFWFGFIGGLTTSAILLTCRLLWLQRNPTRIDMEVDELQIMH

Sequences:

>Translated_460_residues
MHRYQKEIVRLIKLSTPVLIASVAQTGMGFVDTVMAGGVSATDMAAVAIAASVWLPSVLFGVGLLMALVPVVAQLNGSGK
SKKVPFEIHQGVYLALLTSIPIMLVLYNAGFIIAAMDVEPELYEKTQGYLHAVLWAAPAFLLFQTLRSFCEGLSLTTPAM
IIGFIGLAANVPLNWMFVYGELGAPALGGVGCGVATAIVYWLMFLTMTLYTFIAPKLRRVNLYENWNKPQRKEIYRLFKL
GLPVALSIFFEVTLFAAVALLVSPLGSTVVAAHQVAINFSSLIFMIPMSIAVAVSIRVGHKLGEKDLDGAKVASYCGLAF
GLLMACCTAILTLIFREQIAYLYSDNQEVITLAVSLMLLAAIYQCTDAVQVVAAGALRGYKDMNAIFKCTFVSYWIVGLP
SGYVLGMTDWIREPMGVYGFWFGFIGGLTTSAILLTCRLLWLQRNPTRIDMEVDELQIMH
>Mature_460_residues
MHRYQKEIVRLIKLSTPVLIASVAQTGMGFVDTVMAGGVSATDMAAVAIAASVWLPSVLFGVGLLMALVPVVAQLNGSGK
SKKVPFEIHQGVYLALLTSIPIMLVLYNAGFIIAAMDVEPELYEKTQGYLHAVLWAAPAFLLFQTLRSFCEGLSLTTPAM
IIGFIGLAANVPLNWMFVYGELGAPALGGVGCGVATAIVYWLMFLTMTLYTFIAPKLRRVNLYENWNKPQRKEIYRLFKL
GLPVALSIFFEVTLFAAVALLVSPLGSTVVAAHQVAINFSSLIFMIPMSIAVAVSIRVGHKLGEKDLDGAKVASYCGLAF
GLLMACCTAILTLIFREQIAYLYSDNQEVITLAVSLMLLAAIYQCTDAVQVVAAGALRGYKDMNAIFKCTFVSYWIVGLP
SGYVLGMTDWIREPMGVYGFWFGFIGGLTTSAILLTCRLLWLQRNPTRIDMEVDELQIMH

Specific function: Multidrug efflux pump that functions as a Na(+)/drug antiporter

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Homologues:

Organism=Homo sapiens, GI22907060, Length=423, Percent_Identity=22.4586288416076, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI153792564, Length=442, Percent_Identity=24.6606334841629, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI38261960, Length=478, Percent_Identity=23.4309623430962, Blast_Score=93, Evalue=6e-19,
Organism=Escherichia coli, GI48994890, Length=453, Percent_Identity=56.0706401766004, Blast_Score=506, Evalue=1e-144,
Organism=Escherichia coli, GI226510954, Length=457, Percent_Identity=22.7571115973742, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321821, Length=323, Percent_Identity=25.3869969040248, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6320545, Length=423, Percent_Identity=22.9314420803783, Blast_Score=98, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NORM_VIBF1 (Q5E4Y6)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_204798.1
- STRING:   Q5E4Y6
- GeneID:   3278730
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1415
- NMPDR:   fig|312309.3.peg.1415
- eggNOG:   COG0534
- HOGENOM:   HBG479615
- OMA:   DSIQVVA
- PhylomeDB:   Q5E4Y6
- ProtClustDB:   PRK01766
- BioCyc:   VFIS312309:VF1415-MONOMER
- InterPro:   IPR002528
- PANTHER:   PTHR11206
- TIGRFAMs:   TIGR00797

Pfam domain/function: PF01554 MatE

EC number: NA

Molecular weight: Translated: 50151; Mature: 50151

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x10d8ac80)-; HASH(0xcd20444)-; HASH(0x1170e7a8)-; HASH(0x114094ac)-; HASH(0x10ce98b4)-; HASH(0x11505578)-; HASH(0x115fa09c)-; HASH(0x10116f78)-; HASH(0xe82b230)-; HASH(0x10ea9f14)-; HASH(0x107d11cc)-; HASH(0x112443e8)-;

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHEECH
>Mature Secondary Structure
MHRYQKEIVRLIKLSTPVLIASVAQTGMGFVDTVMAGGVSATDMAAVAIAASVWLPSVLF
CCHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GVGLLMALVPVVAQLNGSGKSKKVPFEIHQGVYLALLTSIPIMLVLYNAGFIIAAMDVEP
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCH
ELYEKTQGYLHAVLWAAPAFLLFQTLRSFCEGLSLTTPAMIIGFIGLAANVPLNWMFVYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEEEE
ELGAPALGGVGCGVATAIVYWLMFLTMTLYTFIAPKLRRVNLYENWNKPQRKEIYRLFKL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHCCCCCHHHHHHHHHHH
GLPVALSIFFEVTLFAAVALLVSPLGSTVVAAHQVAINFSSLIFMIPMSIAVAVSIRVGH
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KLGEKDLDGAKVASYCGLAFGLLMACCTAILTLIFREQIAYLYSDNQEVITLAVSLMLLA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AIYQCTDAVQVVAAGALRGYKDMNAIFKCTFVSYWIVGLPSGYVLGMTDWIREPMGVYGF
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHCCHHHHHH
WFGFIGGLTTSAILLTCRLLWLQRNPTRIDMEVDELQIMH
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHEECH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA