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The map label for this gene is opuD [H]

Identifier: 59712001

GI number: 59712001

Start: 1541440

End: 1542996

Strand: Reverse

Name: opuD [H]

Synonym: VF_1394

Alternate gene names: 59712001

Gene position: 1542996-1541440 (Counterclockwise)

Preceding gene: 59712005

Following gene: 59712000

Centisome position: 53.25

GC content: 42.26

Gene sequence:

>1557_bases
ATGTCTACAAATAGTAAGCAAACTAGCGACAAACTTGTTCCAACCCTCACAATAGGATTTATCCTTCTATTTCTTTTAGC
TGCCTTTGTTGATCTCTCAGCATTTACTGCAACTATTCAATCTTTGTTCATGTCGGTTTCGACCATGTTCGGCTATTGGT
GGCAGTGGCTAATGATCATCACTTTTGTTTTAGCTTTTATCATTGCGATGAGCAGTTATGGCAAAATTAAGATGGGCAGT
GAATCAAAACCCACGATAGGTACCTTTCGTTGGGTGGCAATGATCATGTGTACTCTGCTTGCAGGCGGAGGCGTGTTTTG
GTCAGCAGCTGAACCTATTTTCCATTTCATTACACCATCACCAAGCTTTTCTGATGTTAAAGGTGGCACGTTGGCAGCAG
TTGCTCCAGCTTTAAGCCAAAGCTTTTTACATTGGGGATTTTTAGCATGGGCTGTGCTTGGCACATTAAGCACGATTGTT
CTTATGTATGCCCACTATAACTGCGGTGTAAAACTTCGTCCTCGTGCATTGTTATATCCTATTTTGGGTGACAAATTAGA
GAACCATTGGATTGGTTCGGTCATTGATGCTTGTTCTATTATTGCTGTAGCAGCAGGAACCATTGGACCTATTGGATTTT
TAGCAACACAGTTAAGTTACAGTCTGACAGTCCTGACAGGGCTTGAAAGCTCGCTGATGAGCCAAATCATCATTTTGACC
GTGATTGTTAGCATTTATTCTGTTTCGGCCTTCTCTGGCATGGACAAGGGCTTACAGTGGTTGAGTCGTATGAATGTTAT
TGGTGCTGTTGCATTATTAGTTGCGATCTTAGTTCTTGGTCCTACCGGTTTTATCTTTAAAGAGTTCGGTATGGCTTTTG
GTACTTATATTCAGCACTTACCTGAAATGAGCTTGAGTACAGCGGATCCTTCTTGGAATTCGTGGTGGACGTGGTTCTTC
TGGGGATGGTTTATTGGTTTTGCTCCAATGATGGCTATTTTCATTGCACGCATCTCTCAAGGCCGAAGCATTCGTGAGTT
GATTATTACGGTAGCCATTATTGCTCCAATTGCGACCAACTTTTGGTTTTCATCATTGGGTGGAACAGGGATTTTCCTTG
AGCTACAAACTCCGGGTAGCATTTCTCAACCATTGCAAGAAGCGGGCTTACCTGCGGTGTTATTAGCATCATTAGCTCAA
TTACCTTTTAGTTTCTTATTGATACCTGCATTCCTTGTACTTACAACGACGTTTGTAGCAACAACAGGTGATTCAATGGC
TTATTCTATTGCAATGGCAGTAGAGGGCACTGATACACCGTCAAGATATCAGCGTCTATTTTGGGCGGTTATGATGGGTG
TGGTTGCTGGCGTTTTATTGATTGCGGGTGATGGTGGATTAAATGCACTTCAATCCTTTATCGTAATTACAGCTGTACCG
GTTTCTTTCTTGGTTGGTGTTACTTTTATTACTGGACCGATTGCGGTTCTACGTATGAAGAGTGCAGAGCTTGAAAGTAA
ATCATCGGTAGATTGCACAAAAAGCGTTACGGTGTAG

Upstream 100 bases:

>100_bases
TTTTTTTCACTAGAAAATCGGAAAGTTGAAAAATATTTCACTGGTTTTACGTTTTTTAAGCTGCTAGAGTTCCATCCCTC
ACGCATCAAGTGAGATATTT

Downstream 100 bases:

>100_bases
TTATTGATAACGACCCAATAAAACGTTTTGATTGAATGAGAGGCTTATCGCAGAAACTGCGGTAAGCCTTTTTTATTATG
GATAAACGACTATTTCAGGA

Product: glycine betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MSTNSKQTSDKLVPTLTIGFILLFLLAAFVDLSAFTATIQSLFMSVSTMFGYWWQWLMIITFVLAFIIAMSSYGKIKMGS
ESKPTIGTFRWVAMIMCTLLAGGGVFWSAAEPIFHFITPSPSFSDVKGGTLAAVAPALSQSFLHWGFLAWAVLGTLSTIV
LMYAHYNCGVKLRPRALLYPILGDKLENHWIGSVIDACSIIAVAAGTIGPIGFLATQLSYSLTVLTGLESSLMSQIIILT
VIVSIYSVSAFSGMDKGLQWLSRMNVIGAVALLVAILVLGPTGFIFKEFGMAFGTYIQHLPEMSLSTADPSWNSWWTWFF
WGWFIGFAPMMAIFIARISQGRSIRELIITVAIIAPIATNFWFSSLGGTGIFLELQTPGSISQPLQEAGLPAVLLASLAQ
LPFSFLLIPAFLVLTTTFVATTGDSMAYSIAMAVEGTDTPSRYQRLFWAVMMGVVAGVLLIAGDGGLNALQSFIVITAVP
VSFLVGVTFITGPIAVLRMKSAELESKSSVDCTKSVTV

Sequences:

>Translated_518_residues
MSTNSKQTSDKLVPTLTIGFILLFLLAAFVDLSAFTATIQSLFMSVSTMFGYWWQWLMIITFVLAFIIAMSSYGKIKMGS
ESKPTIGTFRWVAMIMCTLLAGGGVFWSAAEPIFHFITPSPSFSDVKGGTLAAVAPALSQSFLHWGFLAWAVLGTLSTIV
LMYAHYNCGVKLRPRALLYPILGDKLENHWIGSVIDACSIIAVAAGTIGPIGFLATQLSYSLTVLTGLESSLMSQIIILT
VIVSIYSVSAFSGMDKGLQWLSRMNVIGAVALLVAILVLGPTGFIFKEFGMAFGTYIQHLPEMSLSTADPSWNSWWTWFF
WGWFIGFAPMMAIFIARISQGRSIRELIITVAIIAPIATNFWFSSLGGTGIFLELQTPGSISQPLQEAGLPAVLLASLAQ
LPFSFLLIPAFLVLTTTFVATTGDSMAYSIAMAVEGTDTPSRYQRLFWAVMMGVVAGVLLIAGDGGLNALQSFIVITAVP
VSFLVGVTFITGPIAVLRMKSAELESKSSVDCTKSVTV
>Mature_517_residues
STNSKQTSDKLVPTLTIGFILLFLLAAFVDLSAFTATIQSLFMSVSTMFGYWWQWLMIITFVLAFIIAMSSYGKIKMGSE
SKPTIGTFRWVAMIMCTLLAGGGVFWSAAEPIFHFITPSPSFSDVKGGTLAAVAPALSQSFLHWGFLAWAVLGTLSTIVL
MYAHYNCGVKLRPRALLYPILGDKLENHWIGSVIDACSIIAVAAGTIGPIGFLATQLSYSLTVLTGLESSLMSQIIILTV
IVSIYSVSAFSGMDKGLQWLSRMNVIGAVALLVAILVLGPTGFIFKEFGMAFGTYIQHLPEMSLSTADPSWNSWWTWFFW
GWFIGFAPMMAIFIARISQGRSIRELIITVAIIAPIATNFWFSSLGGTGIFLELQTPGSISQPLQEAGLPAVLLASLAQL
PFSFLLIPAFLVLTTTFVATTGDSMAYSIAMAVEGTDTPSRYQRLFWAVMMGVVAGVLLIAGDGGLNALQSFIVITAVPV
SFLVGVTFITGPIAVLRMKSAELESKSSVDCTKSVTV

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=527, Percent_Identity=29.0322580645161, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788102, Length=436, Percent_Identity=29.1284403669725, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI1786224, Length=504, Percent_Identity=25.3968253968254, Blast_Score=154, Evalue=9e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 55867; Mature: 55735

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNSKQTSDKLVPTLTIGFILLFLLAAFVDLSAFTATIQSLFMSVSTMFGYWWQWLMII
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFVLAFIIAMSSYGKIKMGSESKPTIGTFRWVAMIMCTLLAGGGVFWSAAEPIFHFITPS
HHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCHHEEECCC
PSFSDVKGGTLAAVAPALSQSFLHWGFLAWAVLGTLSTIVLMYAHYNCGVKLRPRALLYP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHH
ILGDKLENHWIGSVIDACSIIAVAAGTIGPIGFLATQLSYSLTVLTGLESSLMSQIIILT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIVSIYSVSAFSGMDKGLQWLSRMNVIGAVALLVAILVLGPTGFIFKEFGMAFGTYIQHL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
PEMSLSTADPSWNSWWTWFFWGWFIGFAPMMAIFIARISQGRSIRELIITVAIIAPIATN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
FWFSSLGGTGIFLELQTPGSISQPLQEAGLPAVLLASLAQLPFSFLLIPAFLVLTTTFVA
HHHHCCCCCEEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTGDSMAYSIAMAVEGTDTPSRYQRLFWAVMMGVVAGVLLIAGDGGLNALQSFIVITAVP
CCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHH
VSFLVGVTFITGPIAVLRMKSAELESKSSVDCTKSVTV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STNSKQTSDKLVPTLTIGFILLFLLAAFVDLSAFTATIQSLFMSVSTMFGYWWQWLMII
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]