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The map label for this gene is sseA [H]

Identifier: 59711994

GI number: 59711994

Start: 1534676

End: 1535521

Strand: Reverse

Name: sseA [H]

Synonym: VF_1387

Alternate gene names: 59711994

Gene position: 1535521-1534676 (Counterclockwise)

Preceding gene: 59711995

Following gene: 172087703

Centisome position: 52.99

GC content: 38.42

Gene sequence:

>846_bases
ATGAATCAATTATTAGCTGATAACCCATTAGTTACTACCGAGTGGCTAGCAGAACATATTAATGATACTAATCTTGTCAT
TGTTGATGCCTATATGGCGAATATTGTTGGCAAAGAACCGATTGTTTATGACGAGTTTTGTTGTTTACCGAAGTCGCGTA
AGCTTGATGTTGAGTTGGATTTTTGCGATCACGCATCTTCTCAAATTCATGCATTACCAACAGAATCACAGTTTACAAAA
GCCGTTCAAGAGCTTGGCATTAATTCAGATTCCATCGTTGTGATATACGATAACCAAGGGATCTACTCATCTCCACGTGC
TTGGTGGACATTTAAAGTAATGGGGTTTGAGCACGTTTATGTATTAGATGGCGGCTTACCAAAATGGCAAGAAGAAAAGA
GGGAAGTCGTTACTTCTTACCAACAAATACCTTCTTTAGGTAATGCGGTCGCTAAGCCGAATAGGCATTTAGCTTGTGAT
TCAGATGAGATCCTTCACGTTATCGATACAGATGCCGTCATTTTAGATGCACGCGGTGAGATGCGATTTTTAGGTAAAGC
AGCTGAGCCTCGTGAAGGGGTTCGTAGCGGTCATATTCCAAGTTCTAAAAATCTCCCATTTGCTATGTTGTTAGATAATA
CGACCTTTAAAAATAAAGAGATTTTGAACTCGATTTTTCAAGAAAGAGTACCATCAAATATAACAAAGGTATTTTTCAGT
TGTGGTTCAGGCATAACGGCTTGTATTTTAATTTTGGCAGCGGTTTCAATTGGCTATAAAAATGTCGTTTTATACGATGG
TTCATGGGCTGATTGGGGAAGTAATCACAATTTACCGATTGAATAA

Upstream 100 bases:

>100_bases
GTAATCATAAAATAAAAGAAGCGTTAGGTTATGAATTTATTCACCCTGATGTGATGAATATTACGTATGATAGCTAACAG
AGACCAAATAGGGAGTCATC

Downstream 100 bases:

>100_bases
TGAGTTTGTTCTTTGAAGAGTCAAGGGTTAGATAATGCGACAAAGCCTAAGAGATAAAGTAATTGCGGTTTGTGATAAAA
AAATAGAACAAAAGGGAGAT

Product: 3-mercaptopyruvate sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MNQLLADNPLVTTEWLAEHINDTNLVIVDAYMANIVGKEPIVYDEFCCLPKSRKLDVELDFCDHASSQIHALPTESQFTK
AVQELGINSDSIVVIYDNQGIYSSPRAWWTFKVMGFEHVYVLDGGLPKWQEEKREVVTSYQQIPSLGNAVAKPNRHLACD
SDEILHVIDTDAVILDARGEMRFLGKAAEPREGVRSGHIPSSKNLPFAMLLDNTTFKNKEILNSIFQERVPSNITKVFFS
CGSGITACILILAAVSIGYKNVVLYDGSWADWGSNHNLPIE

Sequences:

>Translated_281_residues
MNQLLADNPLVTTEWLAEHINDTNLVIVDAYMANIVGKEPIVYDEFCCLPKSRKLDVELDFCDHASSQIHALPTESQFTK
AVQELGINSDSIVVIYDNQGIYSSPRAWWTFKVMGFEHVYVLDGGLPKWQEEKREVVTSYQQIPSLGNAVAKPNRHLACD
SDEILHVIDTDAVILDARGEMRFLGKAAEPREGVRSGHIPSSKNLPFAMLLDNTTFKNKEILNSIFQERVPSNITKVFFS
CGSGITACILILAAVSIGYKNVVLYDGSWADWGSNHNLPIE
>Mature_281_residues
MNQLLADNPLVTTEWLAEHINDTNLVIVDAYMANIVGKEPIVYDEFCCLPKSRKLDVELDFCDHASSQIHALPTESQFTK
AVQELGINSDSIVVIYDNQGIYSSPRAWWTFKVMGFEHVYVLDGGLPKWQEEKREVVTSYQQIPSLGNAVAKPNRHLACD
SDEILHVIDTDAVILDARGEMRFLGKAAEPREGVRSGHIPSSKNLPFAMLLDNTTFKNKEILNSIFQERVPSNITKVFFS
CGSGITACILILAAVSIGYKNVVLYDGSWADWGSNHNLPIE

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI61835204, Length=274, Percent_Identity=32.1167883211679, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI194473681, Length=274, Percent_Identity=32.1167883211679, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI194473668, Length=274, Percent_Identity=32.1167883211679, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI17402865, Length=272, Percent_Identity=33.8235294117647, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI87082121, Length=275, Percent_Identity=36.3636363636364, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI87081967, Length=221, Percent_Identity=23.9819004524887, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17561888, Length=295, Percent_Identity=27.1186440677966, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71997283, Length=306, Percent_Identity=24.8366013071895, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI115534702, Length=223, Percent_Identity=27.8026905829596, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324825, Length=250, Percent_Identity=30.8, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 31358; Mature: 31358

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQLLADNPLVTTEWLAEHINDTNLVIVDAYMANIVGKEPIVYDEFCCLPKSRKLDVELD
CCCCCCCCCCCHHHHHHHHCCCCCEEEEEHHHHHHCCCCCEEEHHHHCCCCCCCEEEEEE
FCDHASSQIHALPTESQFTKAVQELGINSDSIVVIYDNQGIYSSPRAWWTFKVMGFEHVY
CCCCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEEECCEEEE
VLDGGLPKWQEEKREVVTSYQQIPSLGNAVAKPNRHLACDSDEILHVIDTDAVILDARGE
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEEEEECCCEEEEECCCC
MRFLGKAAEPREGVRSGHIPSSKNLPFAMLLDNTTFKNKEILNSIFQERVPSNITKVFFS
EEECCCCCCCHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHH
CGSGITACILILAAVSIGYKNVVLYDGSWADWGSNHNLPIE
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNQLLADNPLVTTEWLAEHINDTNLVIVDAYMANIVGKEPIVYDEFCCLPKSRKLDVELD
CCCCCCCCCCCHHHHHHHHCCCCCEEEEEHHHHHHCCCCCEEEHHHHCCCCCCCEEEEEE
FCDHASSQIHALPTESQFTKAVQELGINSDSIVVIYDNQGIYSSPRAWWTFKVMGFEHVY
CCCCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEEECCEEEE
VLDGGLPKWQEEKREVVTSYQQIPSLGNAVAKPNRHLACDSDEILHVIDTDAVILDARGE
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEEEEECCCEEEEECCCC
MRFLGKAAEPREGVRSGHIPSSKNLPFAMLLDNTTFKNKEILNSIFQERVPSNITKVFFS
EEECCCCCCCHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHH
CGSGITACILILAAVSIGYKNVVLYDGSWADWGSNHNLPIE
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]