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The map label for this gene is apr [H]

Identifier: 59711956

GI number: 59711956

Start: 1491324

End: 1493066

Strand: Reverse

Name: apr [H]

Synonym: VF_1349

Alternate gene names: 59711956

Gene position: 1493066-1491324 (Counterclockwise)

Preceding gene: 59711958

Following gene: 59711955

Centisome position: 51.53

GC content: 40.79

Gene sequence:

>1743_bases
ATGAATTGTATAACAGCTAAATTAAATAGTTTATTTTCATTGTCTATCATTGTAGTAGCTATGATTTTTTCAATGCAAGT
TAATGCGGTTACTTCCTTAAGGGAAGTCGACGAATCTAACTTCCCGAAAAAATATATTGTTAAATTTAAAGAAGATGCGT
CTAGTTTTTCAGAGTTGAGTGAAATGAGTGAAAACCAGCATGTAACGGAAGAGTTAGTTTTAGAAAAGCTTAAAGCACGT
GAAGTGAAAAGGTTAGGAAACAGTACTATTTACAGTGTGGAGATGGGAGACTTGAAGTTGGAGCAATTGCGTAATCGTAG
CGATGTGGAATACGTAGAAATTGACCAACCCCGTTTTTTATTAAGAAGAGCTTCTCCTGATATATGGAGTGGTGAAACTA
CACCTTGGGGCTATGAGGTGGTTGGGGCTCAGGTTGTTGATGATTTTAATGCAGGTAACCGTACAGTGTGTATCATTGAT
ACAGGTTACGATATAAAGCACAACGATCTGAGTGGTAACCGCGTTTCAGGGCCTAATGTCTCCGATTCTAATTCTGACTT
TCCTTGGAGTGTTCCAGATCCTGGGGGAGATAACAGCCACGGAACTCACGTAGCAGGTACGATCTCTGCGATTGCTAACT
CGGAAGGTATCAAAGGGATCATGCCAAATCAAAAAGTGAATTTGTACATCGTGAAAGCGTTGAATGAAAATGGTTGGGTC
TACTCATCAGATTTGGTGAAAGCCGTACAAACTTGTGTAGACAATGGTGCAAATGTTATCAACATGAGTTTAGGTGGTTC
TAAATCGACAATGACCGAAAAAAACGGGCTACAATCTATCTATGAGAAAGGCGTATTATTAATTGCAGCTGCTGGTAATG
ATGGTGATACTAGTTACTCTTTCCCGGCTTCTTATGATTCCGTAATGTCGGTTGCCGCAGTGGATAGTAATCTTGAACAC
GCAGCTTTTTCTCAAATAACAGACCAAGTTGAAATAGCTGCTCCGGGTGTGGCAATTTTGTCTACCGTTACAGTTGGTGA
AGGTGTGCTTTCAGACATTAGTTTAAACGGTCTTAGTCAGTTTAAGCGTGGTATCGTGCCATACAAGCGTAGGATAATGA
AGGATGAACAATACATTTCAGACCTAGTTACAGGTTCAATCACGGGTATTTTAAAACGTTGTGATTTGTTGTTTGACGGC
ACTTACAAATGTGGTGATATGTCGAACAGTATCTGTTTGATTGAACGCCAGCATGTTAAAAAACAGGATAAAATGTCATT
AGATAATCGCTTGATTAAAACGTGTTCTGATGCTGGGGCAATCGCAGCGATTGTGTATAGTAACGCTAAACTACCAGGCT
TACAGAATCCTTCTATTGTAGATGAAAAAAATGTAGCACGTATTGTCTCAGTAACGGTTGATCATCCCTTTGGTCAGACG
TTATTGGGCTACATAGGTAAAGAGGTCACAGTTTCGACTAGTGAGGGGAAAGACTATGAGTATTACAGTGGTACTTCAAT
GGCGACGCCGCATGTAGCGGGTGTTGCTAGCTTAGTGTGGAGTTATCACCCAACGTGTACCGCAGCACAAGTACGTAGAG
CGCTAACCGAAACCTCAATTGATATTGCCGAAGTAGGCCGTGATAACAGGACTGGTTATGGTATGGTAAATGCTTATGCC
GCAGAACGTTATTTAGATACACTCTGTGGTGAATTGGATTGGGGGCCTTTACCTACGTTATAA

Upstream 100 bases:

>100_bases
TTGCTTTATTGTCACAAGTGTATTTAAGGAGTTAGATTTGAGCTGCTTTGATGCAATAGGAATCAGCTTTCGAATCAGCT
TTCAAAGAGGAATAAATAAA

Downstream 100 bases:

>100_bases
TGTTTTTGATAAATTGAAATTATATACCCTTACATCCTCGGCACAAATCACGAGGATGTAAGCTATTGTTTTATTTTTGT
GTATCAACTGTTTATTAAAA

Product: subtilisin-like serine protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MNCITAKLNSLFSLSIIVVAMIFSMQVNAVTSLREVDESNFPKKYIVKFKEDASSFSELSEMSENQHVTEELVLEKLKAR
EVKRLGNSTIYSVEMGDLKLEQLRNRSDVEYVEIDQPRFLLRRASPDIWSGETTPWGYEVVGAQVVDDFNAGNRTVCIID
TGYDIKHNDLSGNRVSGPNVSDSNSDFPWSVPDPGGDNSHGTHVAGTISAIANSEGIKGIMPNQKVNLYIVKALNENGWV
YSSDLVKAVQTCVDNGANVINMSLGGSKSTMTEKNGLQSIYEKGVLLIAAAGNDGDTSYSFPASYDSVMSVAAVDSNLEH
AAFSQITDQVEIAAPGVAILSTVTVGEGVLSDISLNGLSQFKRGIVPYKRRIMKDEQYISDLVTGSITGILKRCDLLFDG
TYKCGDMSNSICLIERQHVKKQDKMSLDNRLIKTCSDAGAIAAIVYSNAKLPGLQNPSIVDEKNVARIVSVTVDHPFGQT
LLGYIGKEVTVSTSEGKDYEYYSGTSMATPHVAGVASLVWSYHPTCTAAQVRRALTETSIDIAEVGRDNRTGYGMVNAYA
AERYLDTLCGELDWGPLPTL

Sequences:

>Translated_580_residues
MNCITAKLNSLFSLSIIVVAMIFSMQVNAVTSLREVDESNFPKKYIVKFKEDASSFSELSEMSENQHVTEELVLEKLKAR
EVKRLGNSTIYSVEMGDLKLEQLRNRSDVEYVEIDQPRFLLRRASPDIWSGETTPWGYEVVGAQVVDDFNAGNRTVCIID
TGYDIKHNDLSGNRVSGPNVSDSNSDFPWSVPDPGGDNSHGTHVAGTISAIANSEGIKGIMPNQKVNLYIVKALNENGWV
YSSDLVKAVQTCVDNGANVINMSLGGSKSTMTEKNGLQSIYEKGVLLIAAAGNDGDTSYSFPASYDSVMSVAAVDSNLEH
AAFSQITDQVEIAAPGVAILSTVTVGEGVLSDISLNGLSQFKRGIVPYKRRIMKDEQYISDLVTGSITGILKRCDLLFDG
TYKCGDMSNSICLIERQHVKKQDKMSLDNRLIKTCSDAGAIAAIVYSNAKLPGLQNPSIVDEKNVARIVSVTVDHPFGQT
LLGYIGKEVTVSTSEGKDYEYYSGTSMATPHVAGVASLVWSYHPTCTAAQVRRALTETSIDIAEVGRDNRTGYGMVNAYA
AERYLDTLCGELDWGPLPTL
>Mature_580_residues
MNCITAKLNSLFSLSIIVVAMIFSMQVNAVTSLREVDESNFPKKYIVKFKEDASSFSELSEMSENQHVTEELVLEKLKAR
EVKRLGNSTIYSVEMGDLKLEQLRNRSDVEYVEIDQPRFLLRRASPDIWSGETTPWGYEVVGAQVVDDFNAGNRTVCIID
TGYDIKHNDLSGNRVSGPNVSDSNSDFPWSVPDPGGDNSHGTHVAGTISAIANSEGIKGIMPNQKVNLYIVKALNENGWV
YSSDLVKAVQTCVDNGANVINMSLGGSKSTMTEKNGLQSIYEKGVLLIAAAGNDGDTSYSFPASYDSVMSVAAVDSNLEH
AAFSQITDQVEIAAPGVAILSTVTVGEGVLSDISLNGLSQFKRGIVPYKRRIMKDEQYISDLVTGSITGILKRCDLLFDG
TYKCGDMSNSICLIERQHVKKQDKMSLDNRLIKTCSDAGAIAAIVYSNAKLPGLQNPSIVDEKNVARIVSVTVDHPFGQT
LLGYIGKEVTVSTSEGKDYEYYSGTSMATPHVAGVASLVWSYHPTCTAAQVRRALTETSIDIAEVGRDNRTGYGMVNAYA
AERYLDTLCGELDWGPLPTL

Specific function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320775, Length=347, Percent_Identity=26.2247838616715, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319893, Length=205, Percent_Identity=28.780487804878, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324576, Length=237, Percent_Identity=28.6919831223629, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500 [H]

Pfam domain/function: PF00082 Peptidase_S8 [H]

EC number: =3.4.21.62 [H]

Molecular weight: Translated: 63058; Mature: 63058

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNCITAKLNSLFSLSIIVVAMIFSMQVNAVTSLREVDESNFPKKYIVKFKEDASSFSELS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEHHHHHHHHHHH
EMSENQHVTEELVLEKLKAREVKRLGNSTIYSVEMGDLKLEQLRNRSDVEYVEIDQPRFL
HHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHCCCCCCEEEEECCCHHH
LRRASPDIWSGETTPWGYEVVGAQVVDDFNAGNRTVCIIDTGYDIKHNDLSGNRVSGPNV
HHHCCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCEEEEEECCCCEECCCCCCCCCCCCCC
SDSNSDFPWSVPDPGGDNSHGTHVAGTISAIANSEGIKGIMPNQKVNLYIVKALNENGWV
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCE
YSSDLVKAVQTCVDNGANVINMSLGGSKSTMTEKNGLQSIYEKGVLLIAAAGNDGDTSYS
EHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCC
FPASYDSVMSVAAVDSNLEHAAFSQITDQVEIAAPGVAILSTVTVGEGVLSDISLNGLSQ
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHCCCHHHHCCHHHHHH
FKRGIVPYKRRIMKDEQYISDLVTGSITGILKRCDLLFDGTYKCGDMSNSICLIERQHVK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHCC
KQDKMSLDNRLIKTCSDAGAIAAIVYSNAKLPGLQNPSIVDEKNVARIVSVTVDHPFGQT
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHH
LLGYIGKEVTVSTSEGKDYEYYSGTSMATPHVAGVASLVWSYHPTCTAAQVRRALTETSI
HHHHCCCCEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
DIAEVGRDNRTGYGMVNAYAAERYLDTLCGELDWGPLPTL
CHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNCITAKLNSLFSLSIIVVAMIFSMQVNAVTSLREVDESNFPKKYIVKFKEDASSFSELS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEHHHHHHHHHHH
EMSENQHVTEELVLEKLKAREVKRLGNSTIYSVEMGDLKLEQLRNRSDVEYVEIDQPRFL
HHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHCCCCCCEEEEECCCHHH
LRRASPDIWSGETTPWGYEVVGAQVVDDFNAGNRTVCIIDTGYDIKHNDLSGNRVSGPNV
HHHCCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCEEEEEECCCCEECCCCCCCCCCCCCC
SDSNSDFPWSVPDPGGDNSHGTHVAGTISAIANSEGIKGIMPNQKVNLYIVKALNENGWV
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCE
YSSDLVKAVQTCVDNGANVINMSLGGSKSTMTEKNGLQSIYEKGVLLIAAAGNDGDTSYS
EHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCC
FPASYDSVMSVAAVDSNLEHAAFSQITDQVEIAAPGVAILSTVTVGEGVLSDISLNGLSQ
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHCCCHHHHCCHHHHHH
FKRGIVPYKRRIMKDEQYISDLVTGSITGILKRCDLLFDGTYKCGDMSNSICLIERQHVK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHCC
KQDKMSLDNRLIKTCSDAGAIAAIVYSNAKLPGLQNPSIVDEKNVARIVSVTVDHPFGQT
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHH
LLGYIGKEVTVSTSEGKDYEYYSGTSMATPHVAGVASLVWSYHPTCTAAQVRRALTETSI
HHHHCCCCEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
DIAEVGRDNRTGYGMVNAYAAERYLDTLCGELDWGPLPTL
CHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6420308; 9826175 [H]