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The map label for this gene is slt [H]

Identifier: 59711936

GI number: 59711936

Start: 1475382

End: 1477304

Strand: Reverse

Name: slt [H]

Synonym: VF_1329

Alternate gene names: 59711936

Gene position: 1477304-1475382 (Counterclockwise)

Preceding gene: 59711938

Following gene: 59711935

Centisome position: 50.98

GC content: 37.08

Gene sequence:

>1923_bases
ATGCGACTAATGAAAAAAGGATTTGTCGGGTTAATACTCGGGTGTGTTTCAAACAGTGCTTTTGCGATTTCAGAGTTATG
GCTTGATCGTTACGCTTATGATGCGATTCAACAAGGGTTTAATAAAAAAGACATTTCTGTTTATCATAAGAAGCGTGGAG
ATTTAGATAATTATCCATTATCTGGGTATGCTGATTATCGTGCCTTTCTTTTTGATTTAGAGAATAAAACACCAGAGGAA
GTAAATCAGTTTTCAAAAAAGTTTGCTGATTATCCATTCTCCGTTTCCATTCGTGCTGACTACCTTAATGCGTTGATTGC
TACCAAAAATTGGCCATATTTAATCGCTTATCAAACAGAGTTACCTCGTGATCAAGATTACCAATGTTGGTATTACACTG
CTTTTTATCATGAGAATGAATTGGACACCGCCTTTGATGGGGCAAAGCTACTCTGGTTAGATGGTGAGAGTGTCTCTAGC
GCTTGTAATTCCCTTTTTTCATATTGGGATAAGGCCGGTGAACGAACCGATAACATGATACTTGAGCGTGCGTTATACGC
TGTTGATGCAAAGAACCCCAGATTGATTACCTATTTGCGAAAATTAGCCTCTACGGTTGATAGCAAAGACACTTTAACGG
AAATGCTTCGATTGTATCGTCAGCCTGAAAAGTTTACCGTGGTTTTTGAAACCAAAGAGGACACGCAATTTAATCGTAAT
TATGCTCAAACAGCGCTAAAACATTTGGCAAAACATAATATAAATTCCGCTTATGAAAAGGTGGACTTTGTACAGAGTCA
TTTTTCATTTGAAGTTAATGAACTTCAAGCAACACTTGAGTTTCTGGCCGTTAGATTACTGGATACAAAAGATCCTGAAA
TAATCCAATGGAGAGACGATGTTTTTGAACGCTCAAACGATAGTGTCGTTCTAGAGCAACGAATTCGTTTAGCGATTAGA
AAAACGGAATGGAACGAAATTATATTTTGGATTAATAAGCTTACCAGTGAAGAACAAGCATCATATCGATGGCAGTATTG
GTTAGCTCGTAGTGAGATGAAATTAGGGGATACCGAATCTGGTGAAACACGCTTAAAGCGTATCGCTGGTAATAGGCACT
TTTATAGTATTGCAGCATCTATTGCACTAAAAAGAGAACCTCGATTCCCTATTGCTCGTGTTGAGTGTAATAAAGAACAA
CTAGAACCATATCAAGATGATATTGATCGTGTAGAGGAATTACTAGCATTAGGTAAAACGTATCCTGCAAGAAAACATTG
GCGTCATGTAGTCGCAAAAGAGAATCAAGCCAAGAGTGCGGTTTTAGCTTGTTATGCTAAAAAGAAGCATTGGCATTTGT
TTACACATGAAGCCACTATTCGAGGAGAACTATGGGATTATATTTATCTACGTTTTCCTGTTCGCTATTGGAAAGAATTT
ACACGCTATGGCACTAAATATAATGTGGATCCTGTGACATTATTAGCCCTTTCAAGACAAGAAAGTGGTTTAGAGCTAAC
CACTAAATCACCTGTTGGTGCACGTGGATTGATGCAGGTGTTACCAAGTACTGCTGCATTTATGGCACAGAAGTATAATT
TAAATTATCGACATTCGAGTGACTTGTTTGATTTAGATAAAAATGTTGCCGTAGGTAGTCGTTACTTAAGTCATTTGCTT
GCTCGCTATAAGAACAATAGAGCTTACGCATTTTCTGCTTACAATGCAGGACCAACAATGGTGATGGTATGGCGAAGAGC
GGCAAGTAAAGAGATTGATATATTTGGTTATATAGAATCTATTTCATTTAAAGAAACTCGAGTATACGTAAAGAATATTT
TTATGTTTGAAATGTATTATCGAGAAATTTTAAAACTAGAAGGTGATTTCTTAAGACCTTCCGAATTGACCAATTCGATT
TAA

Upstream 100 bases:

>100_bases
AACAAAGTATGCATAGTCGAATTTGTGTGATTTTTTTACGTTATATGGACGTTTTAAACATTCTATACCACACTCATTAA
ATGCAGAAAAAGGATGATTG

Downstream 100 bases:

>100_bases
CCAACAATAGGAATCATTATAGGTATGCACCATCAAGATCAGTTGGCTGAACTGAGTTATAAAACATCACGCCTTATGAT
TGAACATCTAGATTCTGTGA

Product: soluble lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 640; Mature: 640

Protein sequence:

>640_residues
MRLMKKGFVGLILGCVSNSAFAISELWLDRYAYDAIQQGFNKKDISVYHKKRGDLDNYPLSGYADYRAFLFDLENKTPEE
VNQFSKKFADYPFSVSIRADYLNALIATKNWPYLIAYQTELPRDQDYQCWYYTAFYHENELDTAFDGAKLLWLDGESVSS
ACNSLFSYWDKAGERTDNMILERALYAVDAKNPRLITYLRKLASTVDSKDTLTEMLRLYRQPEKFTVVFETKEDTQFNRN
YAQTALKHLAKHNINSAYEKVDFVQSHFSFEVNELQATLEFLAVRLLDTKDPEIIQWRDDVFERSNDSVVLEQRIRLAIR
KTEWNEIIFWINKLTSEEQASYRWQYWLARSEMKLGDTESGETRLKRIAGNRHFYSIAASIALKREPRFPIARVECNKEQ
LEPYQDDIDRVEELLALGKTYPARKHWRHVVAKENQAKSAVLACYAKKKHWHLFTHEATIRGELWDYIYLRFPVRYWKEF
TRYGTKYNVDPVTLLALSRQESGLELTTKSPVGARGLMQVLPSTAAFMAQKYNLNYRHSSDLFDLDKNVAVGSRYLSHLL
ARYKNNRAYAFSAYNAGPTMVMVWRRAASKEIDIFGYIESISFKETRVYVKNIFMFEMYYREILKLEGDFLRPSELTNSI

Sequences:

>Translated_640_residues
MRLMKKGFVGLILGCVSNSAFAISELWLDRYAYDAIQQGFNKKDISVYHKKRGDLDNYPLSGYADYRAFLFDLENKTPEE
VNQFSKKFADYPFSVSIRADYLNALIATKNWPYLIAYQTELPRDQDYQCWYYTAFYHENELDTAFDGAKLLWLDGESVSS
ACNSLFSYWDKAGERTDNMILERALYAVDAKNPRLITYLRKLASTVDSKDTLTEMLRLYRQPEKFTVVFETKEDTQFNRN
YAQTALKHLAKHNINSAYEKVDFVQSHFSFEVNELQATLEFLAVRLLDTKDPEIIQWRDDVFERSNDSVVLEQRIRLAIR
KTEWNEIIFWINKLTSEEQASYRWQYWLARSEMKLGDTESGETRLKRIAGNRHFYSIAASIALKREPRFPIARVECNKEQ
LEPYQDDIDRVEELLALGKTYPARKHWRHVVAKENQAKSAVLACYAKKKHWHLFTHEATIRGELWDYIYLRFPVRYWKEF
TRYGTKYNVDPVTLLALSRQESGLELTTKSPVGARGLMQVLPSTAAFMAQKYNLNYRHSSDLFDLDKNVAVGSRYLSHLL
ARYKNNRAYAFSAYNAGPTMVMVWRRAASKEIDIFGYIESISFKETRVYVKNIFMFEMYYREILKLEGDFLRPSELTNSI
>Mature_640_residues
MRLMKKGFVGLILGCVSNSAFAISELWLDRYAYDAIQQGFNKKDISVYHKKRGDLDNYPLSGYADYRAFLFDLENKTPEE
VNQFSKKFADYPFSVSIRADYLNALIATKNWPYLIAYQTELPRDQDYQCWYYTAFYHENELDTAFDGAKLLWLDGESVSS
ACNSLFSYWDKAGERTDNMILERALYAVDAKNPRLITYLRKLASTVDSKDTLTEMLRLYRQPEKFTVVFETKEDTQFNRN
YAQTALKHLAKHNINSAYEKVDFVQSHFSFEVNELQATLEFLAVRLLDTKDPEIIQWRDDVFERSNDSVVLEQRIRLAIR
KTEWNEIIFWINKLTSEEQASYRWQYWLARSEMKLGDTESGETRLKRIAGNRHFYSIAASIALKREPRFPIARVECNKEQ
LEPYQDDIDRVEELLALGKTYPARKHWRHVVAKENQAKSAVLACYAKKKHWHLFTHEATIRGELWDYIYLRFPVRYWKEF
TRYGTKYNVDPVTLLALSRQESGLELTTKSPVGARGLMQVLPSTAAFMAQKYNLNYRHSSDLFDLDKNVAVGSRYLSHLL
ARYKNNRAYAFSAYNAGPTMVMVWRRAASKEIDIFGYIESISFKETRVYVKNIFMFEMYYREILKLEGDFLRPSELTNSI

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=618, Percent_Identity=30.42071197411, Blast_Score=305, Evalue=4e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 75176; Mature: 75176

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLMKKGFVGLILGCVSNSAFAISELWLDRYAYDAIQQGFNKKDISVYHKKRGDLDNYPL
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCC
SGYADYRAFLFDLENKTPEEVNQFSKKFADYPFSVSIRADYLNALIATKNWPYLIAYQTE
CCCHHHHHEEEECCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHCCCCCCEEEEEECC
LPRDQDYQCWYYTAFYHENELDTAFDGAKLLWLDGESVSSACNSLFSYWDKAGERTDNMI
CCCCCCCEEEEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHH
LERALYAVDAKNPRLITYLRKLASTVDSKDTLTEMLRLYRQPEKFTVVFETKEDTQFNRN
HHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHH
YAQTALKHLAKHNINSAYEKVDFVQSHFSFEVNELQATLEFLAVRLLDTKDPEIIQWRDD
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECHH
VFERSNDSVVLEQRIRLAIRKTEWNEIIFWINKLTSEEQASYRWQYWLARSEMKLGDTES
HHCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCEEEEEEEECCCCCCCCCCC
GETRLKRIAGNRHFYSIAASIALKREPRFPIARVECNKEQLEPYQDDIDRVEELLALGKT
HHHHHHHHHCCCHHEEHHHHHHCCCCCCCCEEEEECCHHHCCCHHHHHHHHHHHHHHCCC
YPARKHWRHVVAKENQAKSAVLACYAKKKHWHLFTHEATIRGELWDYIYLRFPVRYWKEF
CCCHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCEECHHHHEEEHEEHHHHHHHHH
TRYGTKYNVDPVTLLALSRQESGLELTTKSPVGARGLMQVLPSTAAFMAQKYNLNYRHSS
HHCCCCCCCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
DLFDLDKNVAVGSRYLSHLLARYKNNRAYAFSAYNAGPTMVMVWRRAASKEIDIFGYIES
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEHHCCCCCEEEEEEEC
ISFKETRVYVKNIFMFEMYYREILKLEGDFLRPSELTNSI
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
>Mature Secondary Structure
MRLMKKGFVGLILGCVSNSAFAISELWLDRYAYDAIQQGFNKKDISVYHKKRGDLDNYPL
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCC
SGYADYRAFLFDLENKTPEEVNQFSKKFADYPFSVSIRADYLNALIATKNWPYLIAYQTE
CCCHHHHHEEEECCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHCCCCCCEEEEEECC
LPRDQDYQCWYYTAFYHENELDTAFDGAKLLWLDGESVSSACNSLFSYWDKAGERTDNMI
CCCCCCCEEEEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHH
LERALYAVDAKNPRLITYLRKLASTVDSKDTLTEMLRLYRQPEKFTVVFETKEDTQFNRN
HHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHH
YAQTALKHLAKHNINSAYEKVDFVQSHFSFEVNELQATLEFLAVRLLDTKDPEIIQWRDD
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECHH
VFERSNDSVVLEQRIRLAIRKTEWNEIIFWINKLTSEEQASYRWQYWLARSEMKLGDTES
HHCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCEEEEEEEECCCCCCCCCCC
GETRLKRIAGNRHFYSIAASIALKREPRFPIARVECNKEQLEPYQDDIDRVEELLALGKT
HHHHHHHHHCCCHHEEHHHHHHCCCCCCCCEEEEECCHHHCCCHHHHHHHHHHHHHHCCC
YPARKHWRHVVAKENQAKSAVLACYAKKKHWHLFTHEATIRGELWDYIYLRFPVRYWKEF
CCCHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCEECHHHHEEEHEEHHHHHHHHH
TRYGTKYNVDPVTLLALSRQESGLELTTKSPVGARGLMQVLPSTAAFMAQKYNLNYRHSS
HHCCCCCCCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
DLFDLDKNVAVGSRYLSHLLARYKNNRAYAFSAYNAGPTMVMVWRRAASKEIDIFGYIES
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEHHCCCCCEEEEEEEC
ISFKETRVYVKNIFMFEMYYREILKLEGDFLRPSELTNSI
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]