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The map label for this gene is potB [H]

Identifier: 59711923

GI number: 59711923

Start: 1464516

End: 1465373

Strand: Direct

Name: potB [H]

Synonym: VF_1316

Alternate gene names: 59711923

Gene position: 1464516-1465373 (Clockwise)

Preceding gene: 59711922

Following gene: 59711924

Centisome position: 50.54

GC content: 36.83

Gene sequence:

>858_bases
ATGAACAAGAAGTTTAATCTCCAAAATATCATCATCACAATTATTGTTAGTTGGTTACTGCTGTTCGTTTTCATTCCAAA
TGTAATGATCATCGGTACTAGCTTCTTAACTCGTGATGAAGCAAATTTAATCGAAATGACGTTTACGATGGATAACTATC
TACGTCTTTTTGATCCACTATATGCAAAAGTGCTGTGGCATTCATTTTATATGGCCATTGTTGCAACGTTAATCTGTTTG
GTTGTGGGTTACCCATTTGCCTACATCGTTGCAAAAATGCCTGAAAGATGGCGCCCATTTATGCTGTTTTTAATTATTGT
TCCTTTTTGGACTAACTCATTAATCCGTACTTATGGGTTAAAAATTGTGTTAGGTACTCAAGGTATTTTAAATAAAAGCC
TAATTGCAATGGATATCATCGATAAACCATTACGTATTATGTACACAGAAACAGCAGTAATGATTGGTTTAGTTTACATT
CTATTACCTTTCATGATTTTGCCACTGTATTCAGCAATTGAAAAACTTGACGGTACTTACTTAGAAGCGGCTCGTGATCT
AGGTGCAAACAAACTTCAAACTTTGTGGAAGATTGTATTGCCACTAACAATGCCAGGTATTATTGGCGGTTGTTTATTAG
TGTTACTTCCAGCTCTTGGAATGTTCTATATTTCTGACCTATTAGGTGGTGCGAAAAACCTACTAATTGGTAACGTAATT
AAGAGCCAAGTATTGAACGCACGTGATTGGCCATTTGGTGCAGCGACCAGTATCGCCCTCACCTTGGCGATGGCATTAAT
GCTTTATGCTTACTACCGTGCAGGCAAGTTATTAAACAAAAAGGCGGATCTAGACTAA

Upstream 100 bases:

>100_bases
ATCAGTGAGTTCTTTAATGAAGATGATCCAGATGTTGATCACTCTTTAGGTCAAAAAGTAGCAATTACTTGGGTTGAAAG
CTGGGAAGTGGTGTTAGCAG

Downstream 100 bases:

>100_bases
TGGGACGCACAATTAGATTTAGTTTTATGAGTTTGGTATATCTTTTCTTATACCTACCAATCATCGTTCTGATTGCGAAC
TCATTCAACGCAAGTAAATT

Product: spermidine/putrescine ABC transporter membrane protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MNKKFNLQNIIITIIVSWLLLFVFIPNVMIIGTSFLTRDEANLIEMTFTMDNYLRLFDPLYAKVLWHSFYMAIVATLICL
VVGYPFAYIVAKMPERWRPFMLFLIIVPFWTNSLIRTYGLKIVLGTQGILNKSLIAMDIIDKPLRIMYTETAVMIGLVYI
LLPFMILPLYSAIEKLDGTYLEAARDLGANKLQTLWKIVLPLTMPGIIGGCLLVLLPALGMFYISDLLGGAKNLLIGNVI
KSQVLNARDWPFGAATSIALTLAMALMLYAYYRAGKLLNKKADLD

Sequences:

>Translated_285_residues
MNKKFNLQNIIITIIVSWLLLFVFIPNVMIIGTSFLTRDEANLIEMTFTMDNYLRLFDPLYAKVLWHSFYMAIVATLICL
VVGYPFAYIVAKMPERWRPFMLFLIIVPFWTNSLIRTYGLKIVLGTQGILNKSLIAMDIIDKPLRIMYTETAVMIGLVYI
LLPFMILPLYSAIEKLDGTYLEAARDLGANKLQTLWKIVLPLTMPGIIGGCLLVLLPALGMFYISDLLGGAKNLLIGNVI
KSQVLNARDWPFGAATSIALTLAMALMLYAYYRAGKLLNKKADLD
>Mature_285_residues
MNKKFNLQNIIITIIVSWLLLFVFIPNVMIIGTSFLTRDEANLIEMTFTMDNYLRLFDPLYAKVLWHSFYMAIVATLICL
VVGYPFAYIVAKMPERWRPFMLFLIIVPFWTNSLIRTYGLKIVLGTQGILNKSLIAMDIIDKPLRIMYTETAVMIGLVYI
LLPFMILPLYSAIEKLDGTYLEAARDLGANKLQTLWKIVLPLTMPGIIGGCLLVLLPALGMFYISDLLGGAKNLLIGNVI
KSQVLNARDWPFGAATSIALTLAMALMLYAYYRAGKLLNKKADLD

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=275, Percent_Identity=68.7272727272727, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1787080, Length=234, Percent_Identity=39.7435897435897, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1787713, Length=259, Percent_Identity=27.027027027027, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32178; Mature: 32178

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS00589 PTS_HPR_SER ; PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKFNLQNIIITIIVSWLLLFVFIPNVMIIGTSFLTRDEANLIEMTFTMDNYLRLFDPL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEECHHHHHHHHHHH
YAKVLWHSFYMAIVATLICLVVGYPFAYIVAKMPERWRPFMLFLIIVPFWTNSLIRTYGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
KIVLGTQGILNKSLIAMDIIDKPLRIMYTETAVMIGLVYILLPFMILPLYSAIEKLDGTY
EEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LEAARDLGANKLQTLWKIVLPLTMPGIIGGCLLVLLPALGMFYISDLLGGAKNLLIGNVI
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KSQVLNARDWPFGAATSIALTLAMALMLYAYYRAGKLLNKKADLD
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNKKFNLQNIIITIIVSWLLLFVFIPNVMIIGTSFLTRDEANLIEMTFTMDNYLRLFDPL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEECHHHHHHHHHHH
YAKVLWHSFYMAIVATLICLVVGYPFAYIVAKMPERWRPFMLFLIIVPFWTNSLIRTYGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
KIVLGTQGILNKSLIAMDIIDKPLRIMYTETAVMIGLVYILLPFMILPLYSAIEKLDGTY
EEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LEAARDLGANKLQTLWKIVLPLTMPGIIGGCLLVLLPALGMFYISDLLGGAKNLLIGNVI
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KSQVLNARDWPFGAATSIALTLAMALMLYAYYRAGKLLNKKADLD
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]