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The map label for this gene is ycbL [H]

Identifier: 59711801

GI number: 59711801

Start: 1327060

End: 1327713

Strand: Direct

Name: ycbL [H]

Synonym: VF_1194

Alternate gene names: 59711801

Gene position: 1327060-1327713 (Clockwise)

Preceding gene: 59711800

Following gene: 59711802

Centisome position: 45.8

GC content: 40.21

Gene sequence:

>654_bases
ATGAGCTTACAATATCAAATTATTCCTGTGACTTCATACGAGCAAAATTGTTCAATCGTATGGTGTGATGAAACCATGAA
AGGCGTTGTGATCGATCCTGGTGGTGATGTTCAATTGCTGAAACAAGCAATTGAGGCATTAAATGTTGATGTTGTGCATA
TGGTATTAACTCATGGTCATTTAGATCATGTAGGTGGTACAGAAGATCTGGCTGCTATTACTAATGCAACGATTGTTGGT
CCGCATAAAGAAGATAATTTCTGGTTGCAAGGTCTTCCAAACCAAAGTGAGCGATTTGGTTTCCCTCACACAGAGGCATT
TGAGCCAAGCCAATGGTTAAATGAAGGTGATGTGATTGAGTTTGGTAATCAAAAATTAGACGTTCTGCATACGCCGGGTC
ATACTCCAGGTCACGTAGTACTGTTTAATCAAGAAGCAAAAATGGCATTCGTTGGCGATGTACTGTTTAAAGGTGCTATT
GGTCGTTCAGATTTCCCTAAAGGGGACTTTGATACGCTTATTTCTTCTATCAAAACAAAACTGTGGCCATTAGGTAATGA
CATGAGATTTGTACCAGGTCATGGTCCAGAGTCAACATTTGGTCACGAGCGTCAAACTAACCCATTTGTCGCTGATAAAA
TGCCACTTTGGTAA

Upstream 100 bases:

>100_bases
GTCATCAAACGAATGTGATGTGGGATAACCATTGGAATCGAATTCCCCTAAAATAATAGCCGTGCTATTCTTAGTGTAAT
TATTTTAGGAAGAGACAAAT

Downstream 100 bases:

>100_bases
GAAATTTTGTAAAAGTGACGGACTTTTCACCATACAGTGTCGTCATGTCTATAAATATGGCGCGTTTTTTGGTATAAAAC
GCGCTTCGCCAATTCCAATA

Product: metal-binding protein

Products: glutathione; 2-hydroxy carboxylate

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSLQYQIIPVTSYEQNCSIVWCDETMKGVVIDPGGDVQLLKQAIEALNVDVVHMVLTHGHLDHVGGTEDLAAITNATIVG
PHKEDNFWLQGLPNQSERFGFPHTEAFEPSQWLNEGDVIEFGNQKLDVLHTPGHTPGHVVLFNQEAKMAFVGDVLFKGAI
GRSDFPKGDFDTLISSIKTKLWPLGNDMRFVPGHGPESTFGHERQTNPFVADKMPLW

Sequences:

>Translated_217_residues
MSLQYQIIPVTSYEQNCSIVWCDETMKGVVIDPGGDVQLLKQAIEALNVDVVHMVLTHGHLDHVGGTEDLAAITNATIVG
PHKEDNFWLQGLPNQSERFGFPHTEAFEPSQWLNEGDVIEFGNQKLDVLHTPGHTPGHVVLFNQEAKMAFVGDVLFKGAI
GRSDFPKGDFDTLISSIKTKLWPLGNDMRFVPGHGPESTFGHERQTNPFVADKMPLW
>Mature_216_residues
SLQYQIIPVTSYEQNCSIVWCDETMKGVVIDPGGDVQLLKQAIEALNVDVVHMVLTHGHLDHVGGTEDLAAITNATIVGP
HKEDNFWLQGLPNQSERFGFPHTEAFEPSQWLNEGDVIEFGNQKLDVLHTPGHTPGHVVLFNQEAKMAFVGDVLFKGAIG
RSDFPKGDFDTLISSIKTKLWPLGNDMRFVPGHGPESTFGHERQTNPFVADKMPLW

Specific function: Unknown

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI41327741, Length=194, Percent_Identity=30.4123711340206, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI42822880, Length=167, Percent_Identity=31.7365269461078, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI7705793, Length=160, Percent_Identity=30, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1787158, Length=215, Percent_Identity=54.8837209302326, Blast_Score=266, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI17538952, Length=195, Percent_Identity=30.7692307692308, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI221330176, Length=191, Percent_Identity=28.2722513089005, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: 3.1.2.6

Molecular weight: Translated: 24084; Mature: 23953

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQYQIIPVTSYEQNCSIVWCDETMKGVVIDPGGDVQLLKQAIEALNVDVVHMVLTHGH
CCCEEEEEEECCCCCCCCEEEECCCCCEEEECCCCCHHHHHHHHHHHCHHHEEHHHHCCC
LDHVGGTEDLAAITNATIVGPHKEDNFWLQGLPNQSERFGFPHTEAFEPSQWLNEGDVIE
CCCCCCCHHHHHHHCCEEECCCCCCCEEEECCCCCHHHCCCCCCCCCCHHHHCCCCCEEE
FGNQKLDVLHTPGHTPGHVVLFNQEAKMAFVGDVLFKGAIGRSDFPKGDFDTLISSIKTK
ECCCEEEEEECCCCCCCEEEEECCCCCEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LWPLGNDMRFVPGHGPESTFGHERQTNPFVADKMPLW
HCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLQYQIIPVTSYEQNCSIVWCDETMKGVVIDPGGDVQLLKQAIEALNVDVVHMVLTHGH
CCEEEEEEECCCCCCCCEEEECCCCCEEEECCCCCHHHHHHHHHHHCHHHEEHHHHCCC
LDHVGGTEDLAAITNATIVGPHKEDNFWLQGLPNQSERFGFPHTEAFEPSQWLNEGDVIE
CCCCCCCHHHHHHHCCEEECCCCCCCEEEECCCCCHHHCCCCCCCCCCHHHHCCCCCEEE
FGNQKLDVLHTPGHTPGHVVLFNQEAKMAFVGDVLFKGAIGRSDFPKGDFDTLISSIKTK
ECCCEEEEEECCCCCCCEEEEECCCCCEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LWPLGNDMRFVPGHGPESTFGHERQTNPFVADKMPLW
HCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-(2-hydroxyacyl)glutathione; H2O

Specific reaction: S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]