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The map label for this gene is macA [H]

Identifier: 59711789

GI number: 59711789

Start: 1312257

End: 1313291

Strand: Direct

Name: macA [H]

Synonym: VF_1182

Alternate gene names: 59711789

Gene position: 1312257-1313291 (Clockwise)

Preceding gene: 59711788

Following gene: 59711790

Centisome position: 45.29

GC content: 40.0

Gene sequence:

>1035_bases
ATGTTCAAAAAGACTGCAATAGCCGCAGTGCTTGGCTCCATTTTACTCGCAGGCTGTGACAGTAAAGAGCCTAGTATTGC
AGAGCCTGACTCTCGTCCAGCAAAGATCTTAACCGTTTCTGTTGGTGACCACGAAACATCACGCTTGTTTCCAGCTAAAG
CCGAAGCGGGCGATAAAGCCGTTTTGGCTTTTAGAGTTCCTGGACAATTAAATACATTGAATGTACATGCTGGCCAATCG
GTTAGTAAAGGTGAGCTTCTAGCTACAATCAATCCAGATGAATACCGTTTAATCCAGAAACAAGCACAAGCACAATATCG
ATTGATTGATGTGCAATATCAAAGGATTAAAAAGTTAAGAAAAGATAAAGTCGTATCAGAACAAGATTATGATACAGCGG
TTGCCAATAGAAAAACTGCGAAAGCTTCTCTTGACCAAGCCTCTGCTAATTTAAGCTACACTAAGCTTGTCGCACCATAT
GACGGCACGATTTCACTTCTTCCGACAGAAAACTTTGAATATGTAAATGCGAAGCAATCGATTATGCATATTCAAACTAA
CGATATTCTATATGTTGTTTTTCAACTTCCGGATTATTTGCTTCAACGTTTCAGTTTTACAGATGTATCAGCAACAGTTT
CATTTGATTCGTTTCCAAACACTACTTTTCCTCTTGAATTTGAAGAAATTGATACTGAAGCCGATCGTAAAACATCAAGT
TACACGGTAAAAATGAGTATGCCGCGTCCTAAAGATTTAGGGGTTCTTCCGGGGATGTCTGGACAAGTGAAAGTAACTAT
TCCTAAGGGCGGAAGTGAAAAACTGCCACTTTCAGCTATCGATCAAGATGGTGATACCACGTATGTATGGCGTGTGAAAG
AAGATGGAATTGTTGAAAAAGTGGCAGTGAAATTAAATGAGAAACGTCAGGTAGTCTCTGGGATTGAGGATTCAGATCAA
ATTGTATCTTCAGGCATCACAGGGCTAGAAGAGGGAATGAAAGTTCGCAAGTGGGTTAAGGAAAGAGGATTATAA

Upstream 100 bases:

>100_bases
CATTGTTTTTTATAACTAATTTTATTTGTTGACTGGATGAACAAATAAATATAGGCAATAATTCTTTATGTAATCAATTA
ATAATGACAAGGAACGGCAT

Downstream 100 bases:

>100_bases
TAATGCAAAGATTAATGAAACTATCTTTACTTTCCAGCACGTTACTTCTTACTGCTTGTAATCCTGCGCCGACGGAAACT
GTCGTAGAAATTCCGAATGT

Product: acriflavin resistance periplasmic protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MFKKTAIAAVLGSILLAGCDSKEPSIAEPDSRPAKILTVSVGDHETSRLFPAKAEAGDKAVLAFRVPGQLNTLNVHAGQS
VSKGELLATINPDEYRLIQKQAQAQYRLIDVQYQRIKKLRKDKVVSEQDYDTAVANRKTAKASLDQASANLSYTKLVAPY
DGTISLLPTENFEYVNAKQSIMHIQTNDILYVVFQLPDYLLQRFSFTDVSATVSFDSFPNTTFPLEFEEIDTEADRKTSS
YTVKMSMPRPKDLGVLPGMSGQVKVTIPKGGSEKLPLSAIDQDGDTTYVWRVKEDGIVEKVAVKLNEKRQVVSGIEDSDQ
IVSSGITGLEEGMKVRKWVKERGL

Sequences:

>Translated_344_residues
MFKKTAIAAVLGSILLAGCDSKEPSIAEPDSRPAKILTVSVGDHETSRLFPAKAEAGDKAVLAFRVPGQLNTLNVHAGQS
VSKGELLATINPDEYRLIQKQAQAQYRLIDVQYQRIKKLRKDKVVSEQDYDTAVANRKTAKASLDQASANLSYTKLVAPY
DGTISLLPTENFEYVNAKQSIMHIQTNDILYVVFQLPDYLLQRFSFTDVSATVSFDSFPNTTFPLEFEEIDTEADRKTSS
YTVKMSMPRPKDLGVLPGMSGQVKVTIPKGGSEKLPLSAIDQDGDTTYVWRVKEDGIVEKVAVKLNEKRQVVSGIEDSDQ
IVSSGITGLEEGMKVRKWVKERGL
>Mature_344_residues
MFKKTAIAAVLGSILLAGCDSKEPSIAEPDSRPAKILTVSVGDHETSRLFPAKAEAGDKAVLAFRVPGQLNTLNVHAGQS
VSKGELLATINPDEYRLIQKQAQAQYRLIDVQYQRIKKLRKDKVVSEQDYDTAVANRKTAKASLDQASANLSYTKLVAPY
DGTISLLPTENFEYVNAKQSIMHIQTNDILYVVFQLPDYLLQRFSFTDVSATVSFDSFPNTTFPLEFEEIDTEADRKTSS
YTVKMSMPRPKDLGVLPGMSGQVKVTIPKGGSEKLPLSAIDQDGDTTYVWRVKEDGIVEKVAVKLNEKRQVVSGIEDSDQ
IVSSGITGLEEGMKVRKWVKERGL

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=329, Percent_Identity=27.9635258358663, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786668, Length=385, Percent_Identity=25.1948051948052, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082036, Length=315, Percent_Identity=24.4444444444444, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789665, Length=367, Percent_Identity=23.7057220708447, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1789929, Length=374, Percent_Identity=24.0641711229947, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 37982; Mature: 37982

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKTAIAAVLGSILLAGCDSKEPSIAEPDSRPAKILTVSVGDHETSRLFPAKAEAGDKA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCEE
VLAFRVPGQLNTLNVHAGQSVSKGELLATINPDEYRLIQKQAQAQYRLIDVQYQRIKKLR
EEEEECCCCCEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHEEEEEHHHHHHHHHH
KDKVVSEQDYDTAVANRKTAKASLDQASANLSYTKLVAPYDGTISLLPTENFEYVNAKQS
HHCCCCCCCCHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCEECCCCE
IMHIQTNDILYVVFQLPDYLLQRFSFTDVSATVSFDSFPNTTFPLEFEEIDTEADRKTSS
EEEEECCCEEEEEEHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEHHHCCCCCCCCCCE
YTVKMSMPRPKDLGVLPGMSGQVKVTIPKGGSEKLPLSAIDQDGDTTYVWRVKEDGIVEK
EEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEECCCCCEEEEEEECCCCCHHH
VAVKLNEKRQVVSGIEDSDQIVSSGITGLEEGMKVRKWVKERGL
HHHHHCHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFKKTAIAAVLGSILLAGCDSKEPSIAEPDSRPAKILTVSVGDHETSRLFPAKAEAGDKA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCEE
VLAFRVPGQLNTLNVHAGQSVSKGELLATINPDEYRLIQKQAQAQYRLIDVQYQRIKKLR
EEEEECCCCCEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHEEEEEHHHHHHHHHH
KDKVVSEQDYDTAVANRKTAKASLDQASANLSYTKLVAPYDGTISLLPTENFEYVNAKQS
HHCCCCCCCCHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCEECCCCE
IMHIQTNDILYVVFQLPDYLLQRFSFTDVSATVSFDSFPNTTFPLEFEEIDTEADRKTSS
EEEEECCCEEEEEEHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEHHHCCCCCCCCCCE
YTVKMSMPRPKDLGVLPGMSGQVKVTIPKGGSEKLPLSAIDQDGDTTYVWRVKEDGIVEK
EEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEECCCCCEEEEEEECCCCCHHH
VAVKLNEKRQVVSGIEDSDQIVSSGITGLEEGMKVRKWVKERGL
HHHHHCHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]